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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: O0ZE]
#25089760 - 03/25/18 02:28 PM (5 years, 10 months ago) |
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Quote:
O0ZE said: don't mean to interrupt this in depth discussion. I do like what i'm reading and i'm learning all about cloning. seems like hard work. before i go into my update, i might as well clone this jar mushroom i got if it's worth it. what can be said about Jar mushrooms? this one is a beast of a shroom for a jar mushroom. one of the first. growing from WBS. does growing in a jar and being one of the first to grow mean much? does it mean it has harty DNA? i find the little jar mushrooms to be pretty potent, but then again i about lost my mind after making tea with 3.9 grams of dried shroom tea. i couldn't even finish the tea. anyways change of subject...
I increased the temperature of my grow area to about 83F and not the mycelium is responding with growth. i also created some pin holes in my duct tape to allow some GE. feeling more satisfied.
Everything means nothing until you test it
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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anyone spawn too still steaming hot coco?? i completely spaced out while dumping a tote worth of spawn directly into the bucket after like 2hrs
fuckin tard alert
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: cronicr]
#25089772 - 03/25/18 02:34 PM (5 years, 10 months ago) |
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Brilliantly put chron. All I would add is there's a difference between cloning, isolating and stabilizing. This convo is a little all over, cloning isn't hard, isolation and stabilization can be. When I'm underwhelmed with ms potency I look for a better clone
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: mushboy]
#25089774 - 03/25/18 02:35 PM (5 years, 10 months ago) |
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Only once lol...i then dumped some cold water in to cool and added verm n squeezed some out, worked out ok
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Cultivation General Discussion [Re: mushboy]
#25089816 - 03/25/18 02:56 PM (5 years, 10 months ago) |
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Quote:
mushboy said: anyone spawn too still steaming hot coco?? i completely spaced out while dumping a tote worth of spawn directly into the bucket after like 2hrs
fuckin tard alert
It's not ideal, but I've seen grains from used jars survive being washed & dunked in 140+ water, so...
-------------------- Stop cold shocking your mycelium! Hot Spawn - Get It On 
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: grainbrain]
#25089829 - 03/25/18 03:03 PM (5 years, 10 months ago) |
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TheBlackCat
Possibly Human



Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: mushboy]
#25090683 - 03/25/18 10:24 PM (5 years, 10 months ago) |
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So this cluster of pins that came from this mushie that came from this pin. So basically at this point it's just all the same genetic material and probably was after the first clone. It really doesn't matter if I grab one of these and clone it at this point if I already have cultures of this. I'm pretty sure I knw the answer to this. I just wanted to show off how I get better pinsets on agar than I do in my shoe boxes. I still can't believe all those mushies came off of one tiny leftover agar chunk. This is what I have in jars now.
1st

2nd

3rd
Edited by TheBlackCat (03/25/18 10:26 PM)
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Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: TheBlackCat]
#25090950 - 03/26/18 01:27 AM (5 years, 10 months ago) |
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Ive just gotten into agar and isolating a strain, but so far my attempts have been fruitless. I mean, ive done everything by the book and still have this damn trich that is apparently hiding in my otherwise healthy looking isolates.
I isolated one disk through at least 3 transfers and they look great and void of anything weird looking. In fact, everything works like a well oiled machine from inoculation with MS syringe till i put to a coir bulk substrate.
As soon as my cultures go from the agar dish to the bulk trays, trich appears within a couple days. Ive lost 13 tubs in the last 4 months with not one success.
So heres what i did to at least try to get a clone specimen. I took each individual spawn jar and instead of spawnibg to bulk, ive mixed each jar with equal parts of jiffy seed starting mix peat/verm/perlite/ lime and then put about a half inch of the mix on top as a casing.
So far, there is no contamination, only mycelium. This is starting to make me think my contamination is coming from my bulk coir/coffee/verm/ gypsum mix, instead of my agar cultures.
Either way im starting from scratch with fresh materials. The MS syringes i used were 4 years old, but was in the fridge that entire 4 years and i didnt think it would be a problem.
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Cultivation General Discussion [Re: Ghostveil25]
#25091125 - 03/26/18 04:26 AM (5 years, 10 months ago) |
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Quote:
Ghostveil25 said: This is starting to make me think my contamination is coming from my bulk coir/coffee/verm/ gypsum mix, instead of my agar cultures.
Either way im starting from scratch with fresh materials. The MS syringes i used were 4 years old, but was in the fridge that entire 4 years and i didnt think it would be a problem.
Stop putting coffee in your substrate & get some new syringes.
-------------------- Stop cold shocking your mycelium! Hot Spawn - Get It On 
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Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: grainbrain]
#25091141 - 03/26/18 04:47 AM (5 years, 10 months ago) |
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Quote:
grainbrain said:
Quote:
Ghostveil25 said: This is starting to make me think my contamination is coming from my bulk coir/coffee/verm/ gypsum mix, instead of my agar cultures.
Either way im starting from scratch with fresh materials. The MS syringes i used were 4 years old, but was in the fridge that entire 4 years and i didnt think it would be a problem.
Yes, you are correct sir. I just hear so many trusted cultivators like RR saying to add coffee so i guess i was just taking bad advice or outdated advice.
I mean, the grains of coffee in the substrate are pasteurized at 155 degrees in mason jars for 2 hours. So, i thought it would be fine
The syringes definitly must be replaced with fresh ones. This i believe is the source of contamination. Ive gone over and over my sterile and pasteurization techniques and it baffles me as to how anything would be surviving pressure cooking and pasteurization.
Stop putting coffee in your substrate & get some new syringes.
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MiracleMike




Registered: 02/15/16
Posts: 176
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Re: Cultivation General Discussion [Re: Ghostveil25]
#25091175 - 03/26/18 05:40 AM (5 years, 10 months ago) |
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Get new syringes is a good advice, but you can try to get something of your old ones on agar just for fun and practice.
-------------------- Technically alcohol is a solution
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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Re: Cultivation General Discussion [Re: MiracleMike]
#25091445 - 03/26/18 08:48 AM (5 years, 10 months ago) |
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How I feel right before breakthrough...
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 Trade List
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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nice apes
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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Re: Cultivation General Discussion [Re: mushboy]
#25091462 - 03/26/18 08:53 AM (5 years, 10 months ago) |
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This culture has been doing me so well I am considering setting up my 90 or 105 qt mono........ but alas, who doth need that much APE 
I am trying the same clone plate in a shoebox (4 days in). I also cloned a pin from that clone plate (which already was a pin clone) that had a sexy blue head and that culture just went to 2 qt of grains to replace this culture in the bootbox.
I'm loving this so much, what a beautiful variety! I also put MS to agar. Between these babies and trying for golden halo, I'm having fun.
--------------------
 
 Trade List
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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I need that much ape
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: van hatton]
#25091539 - 03/26/18 09:34 AM (5 years, 10 months ago) |
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 gimme all those dicks. gotcha catch em all
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: mushboy]
#25091542 - 03/26/18 09:35 AM (5 years, 10 months ago) |
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Whatchu no about "catching them all"
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: van hatton] 1
#25091545 - 03/26/18 09:37 AM (5 years, 10 months ago) |
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: mushboy]
#25091927 - 03/26/18 01:00 PM (5 years, 10 months ago) |
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When you're a catcher, you know about catchin.
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AtomHeart
MK Ultra Operative



Registered: 05/05/06
Posts: 397
Last seen: 3 years, 1 month
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Re: Cultivation General Discussion [Re: Inocuole]
#25092636 - 03/26/18 05:52 PM (5 years, 10 months ago) |
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Clone question:



So strange...all three are from the same fruit. C1 and C2 appear to be monocultures, with uniform linear mycelium...then C3 is faster growing and rhizomorphic, growing in a slight spiral, and may be sectoring. I figured they would all be the same at least. Anyone know why C3 would be so different? Bacteria?
-------------------- You once ripped a man's jaw off...I seent it!
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