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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: TheBlackCat]
#25087439 - 03/24/18 03:21 PM (5 years, 10 months ago) |
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Quote:
TheBlackCat said: What is exactly meant by recovery. Some people say 48 hours and some 24 hours.
to me, recovery means when the network has re-established itself. so when i shake, within 24hrs, i want the grains to be sticking back together. after 48hrs it should look like i didnt even shake it. thats after 100% colonization..
wbs 24hrs after a shake test

shaking at 10 or 30% is just breaking up the myc mass to get more noc points and then, quicker colonizing.
not technically 'recovery'. its just shaking the grains
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: mushboy]
#25087452 - 03/24/18 03:28 PM (5 years, 10 months ago) |
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Quote:
mushboy said:
Quote:
TheBlackCat said: What is exactly meant by recovery. Some people say 48 hours and some 24 hours.
to me, recovery means when the network has re-established itself. so when i shake, within 24hrs, i want the grains to be sticking back together. after 48hrs it should look like i didnt even shake it. thats after 100% colonization..
wbs 24hrs after a shake test

shaking at 10 or 30% is just breaking up the myc mass to get more noc points and then, quicker colonizing. not technically 'recovery'. just shaking the grains up.
That’s crazy. If my wbs jars could do that after 24 hrs i’d think there was something wrong. I don’t usually get that until 72-96 hours. I always see 48 hrs said as standard for recovery but I’ve never been able to get that. Could it be dry grains after prep/PC? Many other here say that g2g should be done in 6-7 days but it’s more like 12-14 for me. But, I still get quick colonization of subs and badass pinsets and the canopies so what gives? Could you have grains that are too dry so that any culture would colonize slowly until expanded to substrate?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
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Re: Cultivation General Discussion [Re: Germs]
#25087457 - 03/24/18 03:31 PM (5 years, 10 months ago) |
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if i tried that with the oats ive been using lately it would take at least 2-3days. i got more seed colonizing now.
ill post pics after 24hrs. ill be excited to have spawn that doesnt blow donkey dick
EDIT-ALSO.. let me verify that pic is 24hr and not 48
Quote:
mushboy said:
 (no clue why i flipped the jar. must of been high)

hahah thats the original post... whelp. i got wbs colonizing now....
Edited by mushboy (03/24/18 03:38 PM)
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TheBlackCat
Possibly Human



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Re: Cultivation General Discussion [Re: mushboy]
#25088310 - 03/24/18 09:59 PM (5 years, 10 months ago) |
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Well that clears up a lot. Looks more than fine to me. I shook it Thursday at maybe 20-30% around 11pm. This one had the most bits of agar so it colonized the fasted. Everyone else is going to need a shake tomorrow. These jars are from material from that TOC agar pin so I have high hopes for them. I got a few sparse pins in those last 2 KSSS bins and the mycelium just kind of grew over the mold. Still not putting it in my new grow chamber. Just going to harvest what I can get and dump them.
Edited by TheBlackCat (03/24/18 10:00 PM)
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Kyshroomer19xx
Est 19xx soldier



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Re: Cultivation General Discussion [Re: TheBlackCat]
#25089388 - 03/25/18 11:46 AM (5 years, 10 months ago) |
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I finally got a tub of fruits from my first clone i ever did but i have a ? When you grow out a clone and print them does that print carry different characteristics then a regular print of the same variety?
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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tryptkaloids
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Spores are spores. It takes awhile to change the genetics of a print
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Kyshroomer19xx
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25089402 - 03/25/18 11:56 AM (5 years, 10 months ago) |
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But will i see different characteristics and if not whats the reason for cloning if the spores are the same?
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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Explicitdetail
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Clones carry specific traits that we want, but still have 100’s of different genetics running through them. When you go back to MS after a clone you’ve cut down that genetic pool significantly from the pool the clone originally came from. Doing this process over and over allows you to cut the genetic pool every generation selecting the specific traits your looking for.
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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tryptkaloids
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The purpose is for a consistent culture that you can go back to. You are cloning for good live material, not to narrow down the ms crapshoot. Spores will always show random traits. It takes at least 7 generations from spore to clone in order to 'stabilize' phenotypes. If you want a trait to keep showing up you gotta clone for that pheno. Then find a clone with the same pheno within the spores from that clone and so on and so forth. If you want to continually get consistent phenotypes with ms, you will have to do a lot of sifting through ms genetics. Otherwise, you can just grow out a clone indefinately
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Kyshroomer19xx
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So after growing out 1 clone i should look for the characteristics i want and clone again and do that until i get the desired characteristics i am looking for?
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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tryptkaloids
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Quote:
Explicitdetail said: Clones carry specific traits that we want, but still have 100’s of different genetics running through them. When you go back to MS after a clone you’ve cut down that genetic pool significantly from the pool the clone originally came from. Doing this process over and over allows you to cut the genetic pool every generation selecting the specific traits your looking for.
I'm not sure as it's significant as we like to think
Quote:
Kyshroomer19xx said: So after growing out 1 clone i should look for the characteristics i want and clone again and do that until i get the desired characteristics i am looking for?
After each clone (if you want to stabilize a variety) you'll want to go back to spores to narrow down the genetics each generation. I wouldn't do all that work unless I was trying to combine varieties like pasty is whith rustywhyte
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Explicitdetail
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25089477 - 03/25/18 12:31 PM (5 years, 10 months ago) |
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Quote:
tryptkaloids said:
Quote:
Explicitdetail said: Clones carry specific traits that we want, but still have 100’s of different genetics running through them. When you go back to MS after a clone you’ve cut down that genetic pool significantly from the pool the clone originally came from. Doing this process over and over allows you to cut the genetic pool every generation selecting the specific traits your looking for.
I'm not sure as it's significant as we like to think
Yea your probably right I’ll quote from here and say “all that matters is clean spawn”
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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cronicr



Registered: 08/07/11
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Quote:
Explicitdetail said:
Quote:
tryptkaloids said:
Quote:
Explicitdetail said: Clones carry specific traits that we want, but still have 100’s of different genetics running through them. When you go back to MS after a clone you’ve cut down that genetic pool significantly from the pool the clone originally came from. Doing this process over and over allows you to cut the genetic pool every generation selecting the specific traits your looking for.
I'm not sure as it's significant as we like to think
Yea your probably right I’ll quote from here and say “all that matters is clean spawn”
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Explicitdetail
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Re: Cultivation General Discussion [Re: cronicr]
#25089493 - 03/25/18 12:41 PM (5 years, 10 months ago) |
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Damn sorry cron meant to hyperlink that
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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tryptkaloids
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To an extent, I mean environmental factors such as bacterial spawn don't really do anything for potency ime
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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cronicr



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Re: Cultivation General Discussion [Re: tryptkaloids] 1
#25089569 - 03/25/18 01:09 PM (5 years, 10 months ago) |
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I dont know of any proven environmentsl factors that play a role in potency.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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tryptkaloids
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Re: Cultivation General Discussion [Re: cronicr]
#25089632 - 03/25/18 01:36 PM (5 years, 10 months ago) |
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I'm just saying genetics is important too. It just doesn't matter without clean spawn
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Explicitdetail
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25089698 - 03/25/18 02:07 PM (5 years, 10 months ago) |
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I agree that genetics are important but that doesn’t mean someone hasn’t done the work before hand. Unless your dealing with a wild specimen or crossing varieties the pool has been narrowed many times before hand. I agree clones are a great way to get consistent results but it also not a guarantee.
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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O0ZE
Ascended Drone for the hive


Registered: 02/28/18
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don't mean to interrupt this in depth discussion. I do like what i'm reading and i'm learning all about cloning. seems like hard work. before i go into my update, i might as well clone this jar mushroom i got if it's worth it. what can be said about Jar mushrooms? this one is a beast of a shroom for a jar mushroom. one of the first. growing from WBS. does growing in a jar and being one of the first to grow mean much? does it mean it has harty DNA? i find the little jar mushrooms to be pretty potent, but then again i about lost my mind after making tea with 3.9 grams of dried shroom tea. i couldn't even finish the tea. anyways change of subject...
I increased the temperature of my grow area to about 83F and not the mycelium is responding with growth. i also created some pin holes in my duct tape to allow some GE. feeling more satisfied.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Quote:
Explicitdetail said: I agree that genetics are important but that doesn’t mean someone hasn’t done the work before hand. Unless your dealing with a wild specimen or crossing varieties the pool has been narrowed many times before hand. I agree clones are a great way to get consistent results but it also not a guarantee.
Completely agree, most varieties are very stable and the need to isolate is overstated and often not needed, to date my highest producing culture is nowhere near an isolate.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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