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Offlinehamloaf
Loaf of Fam.
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Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Canharo]
    #25063361 - 03/14/18 03:56 PM (5 years, 10 months ago)

Quote:

Canharo said:
Quote:

hamloaf said:
Canharo, are you using a permeable plastic to wrap the edges of your Petri dishes with?




I used glad wrap and double wrapped like I saw on bods agar thread, maybe I should only wrap once or maybe wrapped a little more then I thought?  Im using pyrex petris instead of the plastic ones if that matters



Noticed the glass.  That's cool. 

Myce in 2nd & 3rd picture look healthy enough to transfer from.  There are satellite colonies on that plate so be advised.  Transfer from myce opposite side of satellite colony.  That plate would also be a good candadite for a "josex poke".  Other than that, keep trying.  You're doing alright.

Glad and Saran Wrap both work but are not as pearmeable as plastic polymers such as parafilm.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: hamloaf]
    #25063380 - 03/14/18 04:10 PM (5 years, 10 months ago)

Quote:

hamloaf said:
Glad and Saran Wrap both work but are not as pearmeable as plastic polymers such as parafilm.




What makes you think that?

They're probably more periable lol, at least from what I can find

Parafilm
Gas permeability in 24 hours at 23 °C and 50% relative humidity:
O2 (oxygen): ≤ 350cm³/m²
N2 (nitrogen): ≤ 105cm³/m²
CO2 (carbon dioxide): ≤ 1100cm³/m

I find a lot of different #s for saran and glad films


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OfflineCanharo
Glassblowing Extraordinaire
I'm a teapot


Registered: 10/27/14
Posts: 327
Last seen: 7 months, 29 days
Re: Cultivation General Discussion [Re: Humble Newcomer]
    #25063458 - 03/14/18 04:45 PM (5 years, 10 months ago)

Quote:

Humble Newcomer said:
I've heard the plastic petris have those little steps on them to keep the lid raised and allow for a little GE and glass petris don't have them

I use glass petris and haven't had a problem yet. I wouldn't think that would be an issue for at least two weeks




Yeah I'm not sure either, its been about 8 days since inoculation.  I'm thinking of transfering some over but now I'm worried to open them


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OfflineKyshroomer19xx
Est 19xx soldier
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Registered: 04/22/17
Posts: 1,308
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Last seen: 4 years, 6 months
Re: Cultivation General Discussion [Re: Canharo]
    #25063462 - 03/14/18 04:47 PM (5 years, 10 months ago)

Does anyone wrap pasty plates? Never heard of it but just curious


--------------------
RIP tom petty

Of course it is happening inside your head, but why on earth should that mean it is not real?
Albus  Dumbledore


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Invisiblenatedawgnow
Rocky mountain hood rat
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Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25063464 - 03/14/18 04:48 PM (5 years, 10 months ago)

In the lab i work in, we cover the openings of any glassware like beakers and
flasks with plastic wrap as soon as they come out of the dryer to keep dust
out of them.

As the glass cools it forms a vacuum on the inside that sucks the plastic
wrap into the opening creating a concave bowl. No lie the plastic stays like
that for weeks, sometimes longer, before finally equalizing pressure.

When you remove the plastic wrap you even hear a distinct suction sound.

I prefer plastic wrap but there is no way it's as permeable as parafilm.


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OfflineCanharo
Glassblowing Extraordinaire
I'm a teapot


Registered: 10/27/14
Posts: 327
Last seen: 7 months, 29 days
Re: Cultivation General Discussion [Re: hamloaf]
    #25063476 - 03/14/18 04:53 PM (5 years, 10 months ago)

Quote:

hamloaf said:
Quote:

Canharo said:
Quote:

hamloaf said:
Canharo, are you using a permeable plastic to wrap the edges of your Petri dishes with?




I used glad wrap and double wrapped like I saw on bods agar thread, maybe I should only wrap once or maybe wrapped a little more then I thought?  Im using pyrex petris instead of the plastic ones if that matters



Noticed the glass.  That's cool. 

Myce in 2nd & 3rd picture look healthy enough to transfer from.  There are satellite colonies on that plate so be advised.  Transfer from myce opposite side of satellite colony.  That plate would also be a good candadite for a "josex poke".  Other than that, keep trying.  You're doing alright.

Glad and Saran Wrap both work but are not as pearmeable as plastic polymers such as parafilm.




Thank you Hamloaf I will go threw with the tranfers and see how it goes and let you guys know :heart: You and Bod have really helped me threw my begings in mycology and Ive been hooked since


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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 9 hours, 9 minutes
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Re: Cultivation General Discussion [Re: Canharo]
    #25063516 - 03/14/18 05:17 PM (5 years, 10 months ago)

I have this pint of GT on WBR and I have 6 quarts and a pint of oats ready to inoculate. I'm gonna try to greak it up in the jar so I can just pour it in for G2G. If it doesn't want to crumbe like that, should I use a spoon or maybe cruble by hand with gloves like spawining? I've had so much failure with LC and LI recently, not to mention my rookie Agar adventures, that I feel this is the best uae of my pint instead of spawining a shoebox.

Smacked it with a roll o gorilla tape and it worked quite well enough. I like G2G way better that LC right now hah


Edited by Failboat (03/14/18 05:48 PM)


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InvisibleZero Nowhere
Beer Drinker
Male


Registered: 01/29/18 Happy 6th Shroomiversary!
Posts: 1,471
Loc: Standing on the moon
Re: Cultivation General Discussion [Re: Failboat]
    #25063592 - 03/14/18 05:57 PM (5 years, 10 months ago)

Way off topic!  How do I get my avatar on here?


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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 9 hours, 9 minutes
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Re: Cultivation General Discussion [Re: Zero Nowhere]
    #25063606 - 03/14/18 06:03 PM (5 years, 10 months ago)

Upload a pic and it should give you the option.


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InvisibleZero Nowhere
Beer Drinker
Male


Registered: 01/29/18 Happy 6th Shroomiversary!
Posts: 1,471
Loc: Standing on the moon
Re: Cultivation General Discussion [Re: Failboat]
    #25063614 - 03/14/18 06:06 PM (5 years, 10 months ago)

Thanks bro


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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: Zero Nowhere]
    #25063752 - 03/14/18 07:11 PM (5 years, 10 months ago)

Just spawned 5 shoeboxes and shook up 14 jars. Start to think about switching to bags after that good wrist exercise


--------------------


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Re: Cultivation General Discussion [Re: Germs] * 2
    #25063756 - 03/14/18 07:13 PM (5 years, 10 months ago)

:stonedjerk:


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Invisiblevan hattonFacebookDiscord
Still a noob
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Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Germs]
    #25063759 - 03/14/18 07:13 PM (5 years, 10 months ago)

:feelsbadman:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Offlinehamloaf
Loaf of Fam.
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Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Cultivation General Discussion [Re: Germs] * 1
    #25063786 - 03/14/18 07:22 PM (5 years, 10 months ago)

Quote:

Germs said:
Just spawned 5 shoeboxes and shook up 14 jars. Start to think about switching to bags after that good wrist exercise



Bags are the way to go, brother.
https://www.shroomery.org/forums/showflat.php/Number/20986896#20986896


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleHumble Newcomer
Diddler de niños
I'm a teapot


Registered: 03/12/17
Posts: 1,483
Re: Cultivation General Discussion [Re: hamloaf]
    #25063932 - 03/14/18 08:11 PM (5 years, 10 months ago)

Is it true that psilocybe mexicana does not bruise blue? 

i'm watching "mycology of psilocybin mushrooms - paul stamets - 1998 on reddit while searching the shroomery, i guess that says something about me after work, but anyway at 00:20:45 he's talking about maria sabina and Gordon Wasson and he says " curiously the most popular specimen in that area was psilocybe mexicana, you can jump on this mushroom, it does not bruise blue whatsoever, its very high in psilocybin, very low in psilocin....."

I'm well aware that psilocin is what oxidizes and turns blue, and that not all mushrooms that bruise are active, but i thought however that all actives did bruise??


--------------------
How I made my mush GH


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Humble Newcomer]
    #25063936 - 03/14/18 08:12 PM (5 years, 10 months ago)

It bruises blue. Not only that but paul is the guy who called tampanensis its own unique species but its actually mexicana


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InvisibleHumble Newcomer
Diddler de niños
I'm a teapot


Registered: 03/12/17
Posts: 1,483
Re: Cultivation General Discussion [Re: bodhisatta]
    #25063955 - 03/14/18 08:20 PM (5 years, 10 months ago)

I'm not a big fan and am aware of his book and all its misinformation, but i try to learn where i can.

That just seemed way wrong. In video you can kind of get to see peoples personalities. Many people you wouldn't see much, but Paul loves to play to the crowd, i think thats where that statement stemmed from. This "talk" is a lot of telling stories and playing to a crowd it seems.


--------------------
How I made my mush GH


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OfflineRomeoPapa
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Registered: 05/14/17
Posts: 1,583
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Re: Cultivation General Discussion [Re: hamloaf]
    #25063977 - 03/14/18 08:28 PM (5 years, 10 months ago)

Even with a SAB?


--------------------
It's better to have it and not need it
Than it is to need it and not have it.



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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 9 hours, 9 minutes
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Re: Cultivation General Discussion [Re: RomeoPapa]
    #25064172 - 03/14/18 09:42 PM (5 years, 10 months ago)

Quote:

RomeoPapa said:
Even with a SAB?



I've just started experimenting with bags and I've had no clean results due to various reasons. Next I'll try som G2G in bags done in my SAB.


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InvisibleLemurLemur
Pray for Boog
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Registered: 01/30/17
Posts: 6,004
Loc: Drinking on the roof Flag
Re: Cultivation General Discussion [Re: RomeoPapa]
    #25064411 - 03/15/18 12:08 AM (5 years, 10 months ago)

Check out Frank's write up on it, looks pretty solid.


--------------------
(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]


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