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OfflineAsuraS
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Registered: 08/01/11
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Last seen: 12 days, 15 minutes
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Re: Cultivation General Discussion [Re: dormroomdevient]
    #25027043 - 02/27/18 06:39 PM (5 years, 10 months ago)

Quote:

dormroomdevient said:
the bag is a 3:1 verm brf mix TBH it does look a bit compacted now that I look at it. So this is my idea, roast me if I'm wrong. Would I be able to break up this bag, pull out the substrate, chop it up real good so it isn't compacted, and then follow PF tek with .5 pint jars? I would obviously PC again cause I'm sure it would be full of contams. This seems to make sense in my mind but I also have 1/1000 the understanding of growing shrooms as you guys and gals have lol.

As my handle suggests I'm a student and a bio major. I can't help but hypothesize left and right so rather than run experiments and waste my time and money I'll c if you guys have ever tested the same ideas.

1) When making LC (ik beginners not advised)first I wipe down the whole inside of the jar and the cap with a alcohol wipe then I've been filling a jar with honey (proper weight according multiple LC teks) then adding as much BOILING water as I will need for the LC then putting a cap on it, flipping it upside down while covering the holes on the lid with a pencil eraser (dorm lyfe) and then letting it cool naturally on my desk. The boiling water dissolves the honey real quick and once its cooled down I knock it up. Now b4 any1 skins me alive, this has worked on 3 different LC for me and I even tested all the LC in brf pf tek and it worked no contams just nice myc. So what I'm asking is will this consistently work or did I just get lucky.

2) Tin foil under the cakes or cakes on a raised wire rack? Which is best?

3) I poked an undone wire hanger through the perlite via the holes on the side and bottom of the SGFC which made a bunch of little tunnels in the perlite into which I sprayed a shit ton of water. My misting bottle also squirts a single stream if I turn the nose. Its like a direct injection of water into the perlite, doesn't evap as quick as misting the surface so I would be using it in addition to regular misting but I think it would serve to increase airflow, the 'tunnels' would facilitate more airflow and the water injections would increase perlite saturation so it doesn't go dry too quick. Good idea or bad?

Thanks for bearing with me guys.




Well, I am just practicing here because I am back from a 3 year break, but...

1) No don't do that. PC your jars with whatever media your are going to use for about 20 min
2) Tin foil under the cakes
3) I have no fucking idea what you are talking about. Just put the perlite in as per directed.


Edited by Asura (02/27/18 06:45 PM)


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OfflineMooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 8 days, 4 hours
Re: Cultivation General Discussion [Re: RomeoPapa]
    #25027047 - 02/27/18 06:41 PM (5 years, 10 months ago)

Got some jars with pretty clear signs of bacteria. They smell right but definitely don't look it. Recovered from a shake test in ~60 hours. Top fruit or make shoeboxes?

Example jar:



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OfflineAsuraS
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Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 days, 15 minutes
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Re: Cultivation General Discussion [Re: MooseShroom] * 1
    #25027056 - 02/27/18 06:47 PM (5 years, 10 months ago)

I mostly go by smell. I'm no contam expert, but if I had those jars I would spawn 'em and see
how it played out.


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Offlinetryptkaloids
Learner
I'm a teapot


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Re: Cultivation General Discussion [Re: Asura] * 1
    #25027251 - 02/27/18 08:02 PM (5 years, 10 months ago)

If you are desperate for fruits top fruit them. If you have a good stash then I'd say roll dice with a spawn


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineFishLevelMidnight
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Registered: 09/01/17
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Re: Cultivation General Discussion [Re: cronicr]
    #25027412 - 02/27/18 09:04 PM (5 years, 10 months ago)

Quote:

cronicr said:
Quote:

Asura said:
Quote:

cronicr said:
Nothing luecistic about ape whatsoever snd yes swabs are bruised that isnt spires you are seeing




Please elucidate. This is very interesting.



Ape is a true albino so its spores are clear due to loss of pigmentation unlike luecism where it is a reduction in pigmentation so your spores still stay the proper color, this is why if you want to get a white fruit with gold spores you need to go the luecistic route because any albino will indeed have a loss of pigment and have clear spores.

Also ape and gh are pretty notorious for being on the low end of yield so that novelty wouldnt fly far IMO




Good to know, I will try and make a PE GH cross that produces high potency, decent yield, yellow spores, then try UV irriadiation to see if I can get them to lose fruitbody pigmentation (or see anything else that pops up haha)

I got at least a few years in the hobby where I can be pretty active, might have to give it a shot :smile:

Just spawned my first PE and GH tubs. GH is from MS and the PE is (sadly) from a clones blob culture. Hopefully I will get some spores from each to mix-a-roonie!


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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Cultivation General Discussion [Re: FishLevelMidnight]
    #25027422 - 02/27/18 09:08 PM (5 years, 10 months ago)

The beauty of cubes is that they are cubes...it doesnt only have to be 2 varieties


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineFishLevelMidnight
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Re: Cultivation General Discussion [Re: cronicr]
    #25027441 - 02/27/18 09:19 PM (5 years, 10 months ago)

What do you mean?

If I were to mix 3 varieties (lets say AA+, GH, PE) wouldn't I have a really low chance of getting my desired phenotype in the F2?

I'm actually not entirely sure how that all would work in terms of spores being incorporated into the mycelium network ad donating their genetics.


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: FishLevelMidnight]
    #25027452 - 02/27/18 09:26 PM (5 years, 10 months ago)

Maybe maybe not...you dont nprmally see much in your f1 and f2


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineFishLevelMidnight
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Re: Cultivation General Discussion [Re: cronicr]
    #25027513 - 02/27/18 10:08 PM (5 years, 10 months ago)

good to know. In the few posts I've really read (workman mostly) I thought some phenos were visible by the F2 (isn't that the APE story?)


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: FishLevelMidnight]
    #25027534 - 02/27/18 10:19 PM (5 years, 10 months ago)

Yep often will be but by far not stable, i still get black spores often in my f2


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinePsilosopherr
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Re: Cultivation General Discussion [Re: cronicr]
    #25027651 - 02/27/18 11:39 PM (5 years, 10 months ago)

alright i give up, whats f2/f1?


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: Psilosopherr] * 2
    #25027659 - 02/27/18 11:45 PM (5 years, 10 months ago)

Filial generation.  F2 would be the offspring of f1 generation.  F3 would be the offspring of f2 generation, etc.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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Offlineleafygreens
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Re: Cultivation General Discussion [Re: hamloaf]
    #25027684 - 02/28/18 12:04 AM (5 years, 10 months ago)

So I made some transfers from pe6 prints I made last year, I keep getting tomemtose growth, on 3rd transfer from original print I just took the best plates and made 6 quart jars.

Also kept some plates I first started on and made a few pin transfers of that pe6 prints.

I used 7g agar bar with 1.5 liter wbs grain water for 4th transfer while I soaked those jars I made I siad why not a 4th with grain water but not sure if I added honey cus the other 3 transfers were PDA pasty plates. May I have been using too much nutes?


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Edited by leafygreens (02/28/18 12:14 AM)


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: leafygreens]
    #25027686 - 02/28/18 12:07 AM (5 years, 10 months ago)

Genetics are a factor.  High nutritional content encourages tomemtose mycelial growth, too.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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Offlineleafygreens
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Re: Cultivation General Discussion [Re: hamloaf]
    #25027700 - 02/28/18 12:20 AM (5 years, 10 months ago)

Hopefully they colonize well, I cant seem to upload pics but the agar I threw into my wbs are already colonizing what seeds are around it looking clean so far 4 days


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Offline00Burnout
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Re: Cultivation General Discussion [Re: leafygreens]
    #25028674 - 02/28/18 12:36 PM (5 years, 10 months ago)

Got some agar, li, and lc in the pc. Gonna noc up some qts with coprinus comatus and azurescens and take some transfers.


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Offlinedormroomdevient
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Re: Cultivation General Discussion [Re: 00Burnout]
    #25029153 - 02/28/18 02:14 PM (5 years, 10 months ago)

I asked this yesterday but I didn't get an answer and now I'm afraid this big thread swallowed it up haha. So I've got a bunch of brf substrate made up in a bag and I'm realizing it probably won't colonize, too compacted. So in an attempt to rescue some of that substrate and not just toss it all can I break it up and the put it in jars and PC again? I just don't wanna waste all the substrate you know.

If this is not gonna work I wanna know why nothing is more annoying than when some1 just says 'No you noob' I don't learn then.

Thanks:thumbup::mushroom2:


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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: dormroomdevient]
    #25029191 - 02/28/18 02:22 PM (5 years, 10 months ago)

If its in a bag just crumble it up :shrug: pcing agaun may fuck up the moisture content


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Offline00Burnout
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Re: Cultivation General Discussion [Re: natedawgnow]
    #25029208 - 02/28/18 02:28 PM (5 years, 10 months ago)

You could try to lay the bag on it's side and spread it out


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No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: 00Burnout]
    #25029479 - 02/28/18 04:22 PM (5 years, 10 months ago)

I would just let it do it's thing until it stalls then message the bag. To loosen shit up. Hopefully its not bacterial


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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