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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Crispykoot]
#24996434 - 02/15/18 12:47 PM (5 years, 11 months ago) |
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Really? Fuck I actually gave up on my search for real petris long ago because I could not find any vendor that sold them near me at a reasonable price. I'm very happy with my current no-pour containers and wouldn't change them for real petris even if I had them for free, but just out of curiosity, got a link?
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 16 hours, 58 minutes
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Re: Cultivation General Discussion [Re: Josex]
#24996445 - 02/15/18 12:50 PM (5 years, 11 months ago) |
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--------------------

Shadowboxing the apocalypse and wandering the land
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Crispykoot]
#24996448 - 02/15/18 12:54 PM (5 years, 11 months ago) |
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I see they have distributors all around the world, but couldn't check if it's really a Spanish manufacturer. Thanks for the link though, may give those a whirl on a whim some time in the future.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Josex]
#24996454 - 02/15/18 12:55 PM (5 years, 11 months ago) |
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Quote:
Josex said: That bag in the pic was opened 2 years ago. Expiration date long due. LME doesn't go bad, it can clump together, but you could in theory still use it, only that the moisture content in clumped-up LME can fuck with the measurements when you weigh it out.
I stand corrected. I remember reading that if it's clumped, enough moisture has been absorbed that it shouldn't be used. After searching again now, I can't find it or anything supporting it. Thanks for the tip on closing the bag. I'll probably move mine from small jar to double bagged and back into big jar.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Quote:
van hatton said: Dude. I've been doing just dry and my tan problems are slowly disappearing.
While practicing is getting me better the basics pretty much stayed the same.
Stare... Star... The jars from the video are looking great except for one. But it's apparent it's from filter failure.
I'm surprised the "pouring" jar didn't tam at all. That was originally why I didn't do it that way (I've watched your video a few times. ) fukkit.
Yes, your filters were one of the culprits I suspected early on, along with how fast you close your jars. You might want to make some SFD lids. If you're still having trouble with inoculating, whether liquids or G2G, let me know and I can post better videos.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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I only feel like a couple of my filters aren't up too par. They are rock hard once I get it in there tightly. I did like 70 lids in a couple hours so some probably aren't the best.
I have sfds. I've had polyfil outlast sfds on average. I still have a quart jar with agar in it sfd probably 7 months old or so. The other sfds failed that one just doesn't give up at the end of that "test" I saw sfds not working as well as polyfil. I know science say I'm Full of shit but that's just what I saw. All jars were uninnoculated.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24996537 - 02/15/18 01:42 PM (5 years, 11 months ago) |
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i keep my lme in a tupperware in my panty with the flour and other shit.
Quote:
tryptkaloids said: I've read that malt is the clearest option.

 the lines are from the LED work light. i guess it fucks with the cameras sensor but the agar is clear as glass.
cold water hydration. just shake and pc 20min
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: van hatton]
#24996556 - 02/15/18 01:56 PM (5 years, 11 months ago) |
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Quote:
van hatton said: I only feel like a couple of my filters aren't up too par. They are rock hard once I get it in there tightly. I did like 70 lids in a couple hours so some probably aren't the best.
I have sfds. I've had polyfil outlast sfds on average. I still have a quart jar with agar in it sfd probably 7 months old or so. The other sfds failed that one just doesn't give up at the end of that "test" I saw sfds not working as well as polyfil. I know science say I'm Full of shit but that's just what I saw. All jars were uninnoculated.
Well at the very least, stop pulling your lids and ring off by the SFD. That can't be optimal, both from agitating the poly barrier and clunking around in your SAB.
How were you putting your SFDs on the jars? Early on, I just put a small bit on the underside edges and stuck it down. They come off easy, aren't locked down, and likely allow shit to sneak under. Now I use a solid amount of RTV on the whole outer edge, including the top, so it's firmly locked into place and there's no space between SFD and lid.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 11 hours
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I do both of those things. I circle the hole slap the add on and give it a generous bead around the outside making,sure it has a lip over the top. I think the #1 reason for add failure is really just silicone failure
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24996574 - 02/15/18 02:05 PM (5 years, 11 months ago) |
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When I noc up jars I stack my rings on a piece of foil, pop the seal with the dull side of my flamed exacto and use the poly to lift the lid. I haven't had any problems so far.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: 00Burnout]
#24996598 - 02/15/18 02:20 PM (5 years, 11 months ago) |
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What you're describing is much different than what I saw in his vid, for sure. IIRC, he was unscrewing the ring, but leaving it on, and then using the Poly to pull both the lid and dangling ring off the jar.
Using the blade is interesting. I don't love using my fingers, but I'd be afraid using an instrument would plop them up too forcefully sometimes. Even with SFDs, I get a little vacuum once in a while. But it clearly can work if you're doing it.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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I saw someone (can't find video) do that think it was munch.
When I have a vacuum I use flamed hemos. With them flipped upside down there no vacuum.
The whole point of that was too be fast lol. That's why it was picked up and put down so quickly. I tried it slowly same results.
Just poured some king oyster lc well see how it goes
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24996612 - 02/15/18 02:29 PM (5 years, 11 months ago) |
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Quote:
tryptkaloids said: I've read that malt is the clearest option. I dont mind sediment but,it would,be nice to view my cultures from underneath
Why not use grain soak agar?
I never saw the point in buying nutrients for agar when I'm producing it all the time anyways. I've been doing about half strength from a batch of like 4 qts of grain. I've used as low as 1/5 GW to have much clearer plates and still get growth.
Considering how OCD I am I am surprised I don't care about knowing the nute content exactly; butfor my purposes agar is simply for storage and viewing cultures. I don't need to mess around with the dogma surrounding agar and transfers and nutes and all that.
--------------------
 
 Trade List
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: van hatton]
#24996651 - 02/15/18 02:45 PM (5 years, 11 months ago) |
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Quote:
van hatton said: The whole point of that was too be fast lol. That's why it was picked up and put down so quickly. I tried it slowly same results.
The speed at which you move isn't the best metric to focus on. A better goal is minimizing substrate exposure, but not at the expense of kicking shit up or making mistakes. You can also optimize your technique further, e.g., only open receiving jars the minimum width needed to inoculate, position the master (jar, syringe, scalpel) closer to the recipient before opening to minimize exposure time, using jars/inoculant quickly after sterilizing/sanitizing, etc. It's obviously not always just one thing.
I'm definitely no expert, just notice that I put my lids on in a more controlled manner than you and seem to have a much lower contam rate. Definitely doesn't mean this is the vector fucking your shit up or that it's definitely an issue, of course. But if your agar is sterilized properly and your filters aren't failing, you're obviously knocking shit in there somehow.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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I almost used my fingers to pop the seal on 2 piece lids, but I got to thinking how easily it would be to have even the tiniest piece of glove get under the lid.
There are definitely many ways to get shit done in this hobby, and not everything will work for everyone, that's for sure.
I'm thinkin I might try my hand at g2g soon, other than slurries it's the only method of inoculation I haven't tried.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: 00Burnout]
#24996727 - 02/15/18 03:21 PM (5 years, 11 months ago) |
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G2G is absolutely a must-have in your arsenal. It's the most efficient way to rapidly expand your spawn. Here's a few things to consider.
- A regular mouth master jar is optimal. I prefer RM master to WM recipient.
- Make sure you'll have enough room to maneuver in there, specifically WRT height. For most SABs, quart to quart transfers aren't an easy fit, so people use pint jars very often. Obviously a hood or big SAB could change this.
- It's worth leaving a clump of grains in the jar to smell after. My first attempts at G2G were all fucked because I was treating my master jars like spawn jars and smelling before using. Obviously don't do that.
- There IS a point where you've expanded to the point of noticeable senescence. This point isn't set in stone and varies depending on a plethora of factors, but it will happen.
- I prefer to use smaller grain as a master due to more inoculation points, e.g., straight millet or WBS with corn/seeds filtered out.
- I'll often expand the initial pint master into more pints before moving to quarts for spawn, or I'll go 1 pint > 5 quarts & 1 pint > 5 quarts & 1 pint, etc. I believe Pasty told me about this early on.
You probably know all of this, but others might not so it's worth typing up. G2G is amazing and will make more spawn with less effort than you know what to do with.
Slurries are fun, too. LI and slurries were the very first inoculants I learned and your spawn output will be unreal when used in tandem with G2G. I'm going to make some more BRF cakes soon just to slurry again.
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: 00Burnout]
#24996743 - 02/15/18 03:30 PM (5 years, 11 months ago) |
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Stare did you see Mateahβs experimet on plates left open for varying amounts of time?
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: JHOVA]
#24996755 - 02/15/18 03:36 PM (5 years, 11 months ago) |
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Yep, I commented. I'd planned on doing a similar test with G2G; wanted to leave jars open for different intervals before dumping grains in. I've successfully G2G'd a master with the lid off the entire time for 5 jars before. It wasn't going to really be a test, so to speak, but more so (hopefully) a visual confirmation that your technique needs to be solid + your SAB needs to be still, not sterile.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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I've been loving lc lately. I've got an atl7 lc and 2 columbinus lc's, one is a clone.
I make and pour my lc from wm qt jars with no problem, 6 jars at a time in my 66qt sab. It's definitely crowded, but working in a crowded sab has helped me tremendously with my sterile tek as I have to visualize every single jar placement and movement, it's made me so much more aware of my work.
I'll definitely get some regular mouth pints for g2g. Since I primarily do stones it's gonna be a little tricky, I already get 100% in 5-7 days with my lc and I know I'll miss my best opportunity to g2g.
I might leave g2g to my other species. Right now my print and culture library consists of ps cubensis var "gt" and "purple mystic", ps alutaceae, ps mexicana var "tampenensis" and "galindoi (atl7), gymnopilus thiersii, pl columbinus, pl citrinopileatus, and coprinus comatus. I also might be getting ps azurescens, and ps caerulescens soon.
Fuck idk how I'm going to coordinate all this, especially since all my active species except mexicana is going outdoors and I plan on moving this fall. I might try sneaking a few trays of gyms, caerulescens, and alutaceae in my gh, although I think it's gonna be too warm for alutaceae in my gh before it's ready to fruit.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: 00Burnout] 1
#24996795 - 02/15/18 03:54 PM (5 years, 11 months ago) |
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100% in 5-7 days is great. You can G2G myc with stones forming, btw. But yeah man, like you know, everyone's situation is different and it's all about finding what's optimal for you. Definitely agreed re: visualizing moves in a SAB. That's something that I focused early on. Even now when I'm doing different shit in there, I'll take a bit ahead of time to map out in my head how I want things to work in there.
SAB work is like shooting pool, your moves should be made in mind with setting up the next. That's I yell out, "SCALPEL BLADE, CORNER POCKET!" before transferring to new plates. And then I'll make bets with myself. "Whadd'ya say? Let's make it interesting. $20 per wedge?" One time during a real big session my myc slipped off the blade onto the towel below. I couldn't come up with the money so I broke my own thumbs. Couldn't do myco-related stuff for 2 months. Sometimes I keep it too real.
Anyway, cool library.
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