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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: 00Burnout]
#24990279 - 02/12/18 11:42 PM (5 years, 11 months ago) |
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Burnout is right. If you don't shake the liquid it just pools at the bottom. But with an agar wedge, it's apparently best to wait because the myc has to crawl off the wedge onto grain before you can shake and distribute it. Otherwise you're just beating up myc before it has a chance to do anything.
Edited by stareatclouds (02/12/18 11:50 PM)
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Why don't we use iodine 's ISO for our hands?(gloved) Besides it turning our wrist a weird color 
Just a thought
Edit Nvm ISO is better than iodine thanks google.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (02/12/18 11:54 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Cultivation General Discussion [Re: 00Burnout]
#24990290 - 02/12/18 11:51 PM (5 years, 11 months ago) |
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Quote:
00Burnout said:
Quote:
Shomann said: Is it LI or LC you guys aren't shaking after inoculation?
LI right? Because we've already blended it up and shaking it is just beating it up more?
I'm pretty sure you shake after inoc with both, atleast I do, to further distribute inoculation points. I've always heard you don't shake with wedges.
You can shake with all 3...the only reason not to is if ya wanna judge your 30% to shake again
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Changa Alchemist
Shwift sauce lifeguard


Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Re: Cultivation General Discussion [Re: cronicr]
#24990339 - 02/13/18 12:12 AM (5 years, 11 months ago) |
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Right on guys.
I was just dropping a few wedges and using LI via blender tek and I remember mushboy talking about how he got yelled at for shaking after one of the liquid inoculation methods.
Thanks for the input guys.
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pacmanbreed


Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
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Mateh, stripe, stare, cron thanks brother.

 Man they are fast. doubled in size in 8hrs Wipping my agar now. hope still have time for transfer. Ill make sure nexttime ill have a ready made plates..
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TheBlackCat
Possibly Human



Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: pacmanbreed]
#24990476 - 02/13/18 01:54 AM (5 years, 11 months ago) |
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I actually made a video of me working for the first time. But youtube is stupid and won't let me post it because of the music in the background. I could put a song over it but then you can't hear what I'm saying. I'll try again another time.
Also does tall skinny mushies indicate an FAE issue? I do have some meat ones but also getting a lot of tall skinny ones. I'll have to rig up a way for it to get some better air exchange. Anyone have any suggestions for good programmable timers? Something where I could turn the air on for a few minutes at a time.


Edited by TheBlackCat (02/13/18 03:19 AM)
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24990710 - 02/13/18 06:53 AM (5 years, 11 months ago) |
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Quote:
TheBlackCat said: I actually made a video of me working for the first time. But youtube is stupid and won't let me post it because of the music in the background. I could put a song over it but then you can't hear what I'm saying. I'll try again another time.
Same shit happened to me last week, made a 15 min video of a dry SAB sesh and had parks and rec playing in the background, not even music
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Steevo
Just a cog in the death wheel



Registered: 03/02/17
Posts: 2,702
Loc: Here and There
Last seen: 1 year, 9 months
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Re: Cultivation General Discussion [Re: Germs]
#24990780 - 02/13/18 08:00 AM (5 years, 11 months ago) |
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Quote:
TheBlackCat said: I actually made a video of me working for the first time. But youtube is stupid and won't let me post it because of the music in the background. I could put a song over it but then you can't hear what I'm saying. I'll try again another time.
Also does tall skinny mushies indicate an FAE issue? I do have some meat ones but also getting a lot of tall skinny ones. I'll have to rig up a way for it to get some better air exchange. Anyone have any suggestions for good programmable timers? Something where I could turn the air on for a few minutes at a time.


Check out Hurricane Axial fan on Amazon. Cut a hole and mount it to the plastic blowing out. Set it on a timer to circulate the air out. Consider spores and venting a pipe outside so you’re not filling the rest of your place up with them
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flowstate
Stranger
Registered: 01/03/18
Posts: 12
Last seen: 5 years, 9 months
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Re: Cultivation General Discussion [Re: Steevo]
#24990806 - 02/13/18 08:19 AM (5 years, 11 months ago) |
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I accidentally ordered mini-petri dishes.
Anyone have any experience with these little guys? I'm sitting here looking at them like I may be on to something.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: flowstate]
#24990818 - 02/13/18 08:31 AM (5 years, 11 months ago) |
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They're great for mailing cultures on a plate.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Germs]
#24990823 - 02/13/18 08:35 AM (5 years, 11 months ago) |
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Quote:
Germs said:
Quote:
TheBlackCat said: I actually made a video of me working for the first time. But youtube is stupid and won't let me post it because of the music in the background. I could put a song over it but then you can't hear what I'm saying. I'll try again another time.
Same shit happened to me last week, made a 15 min video of a dry SAB sesh and had parks and rec playing in the background, not even music
i turned my camera mic off for that reason i dont want dio's estate sending me a cease and desist
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Mycolorado]
#24990824 - 02/13/18 08:36 AM (5 years, 11 months ago) |
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Quote:
Mycolorado said: They're great for mailing cultures on a plate.
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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Re: Cultivation General Discussion [Re: flowstate]
#24990831 - 02/13/18 08:42 AM (5 years, 11 months ago) |
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Quote:
flowstate said: I accidentally ordered mini-petri dishes.
Anyone have any experience with these little guys? I'm sitting here looking at them like I may be on to something.
They can be great if you use in vitro pins/fruit for cloning.
My grow plan is to clean up any variety on agar and then let it fruit, clone the largest and first forming pin, then use half of that cloned pin culture for growing (the other half gets put in the fridge for future storage).
If you use these small plates and transfer a clean piece of myc to them, they will colonize and pin in much less time than a regular sized plate...
--------------------
 
 Trade List
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: cronicr]
#24990874 - 02/13/18 09:18 AM (5 years, 11 months ago) |
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Quote:
cronicr said: You can shake with all 3...the only reason not to is if ya wanna judge your 30% to shake again
So it's bullshit that shaking a wedge immediately is detrimental because it means the myc still has to recolonize the agar wedge before it leaps off?
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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If it's healthy mycelium it shouldn't matter. It recovers and starts colonizing stuff within 24 hours. Just because you can't see it doesn't mean it's happening.
One reason I see is to see if tams pop up. Shouldn't be a problem if your technique is on point.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Quote:
stareatclouds said:
Quote:
cronicr said: You can shake with all 3...the only reason not to is if ya wanna judge your 30% to shake again
So it's bullshit that shaking a wedge immediately is detrimental because it means the myc still has to recolonize the agar wedge before it leaps off?
Yep i tested this a couple years ago, dropped about 1/4 plate and shook for a few jars...i mean all your doing is dropping something clean in a jar of grain
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Quote:
stareatclouds said:
Quote:
cronicr said: You can shake with all 3...the only reason not to is if ya wanna judge your 30% to shake again
So it's bullshit that shaking a wedge immediately is detrimental because it means the myc still has to recolonize the agar wedge before it leaps off?
Yea, it spreads inoculation points. Stamets even recommends it in the GGMM, but I never do it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: cronicr]
#24990982 - 02/13/18 10:18 AM (5 years, 11 months ago) |
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i only hold off on shaking so i can see where possible contams originate.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Cultivation General Discussion [Re: mushboy]
#24990989 - 02/13/18 10:23 AM (5 years, 11 months ago) |
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Quote:
mushboy said: i only hold off on shaking so i can see where possible contams originate.
This is why we use agar ...knock that paranoid shit off Jk i dont bother to shake so i can judge the 30 % better and to boot the more inoculation points thing never really added up to faster colonizing with shaking a wedge
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: van hatton]
#24991024 - 02/13/18 10:39 AM (5 years, 11 months ago) |
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Quote:
van hatton said: If it's healthy mycelium it shouldn't matter. It recovers and starts colonizing stuff within 24 hours. Just because you can't see it doesn't mean it's happening.
One reason I see is to see if tams pop up. Shouldn't be a problem if your technique is on point.
There's really no benefit. Takes just as much time to colonize either way.
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