Home | Community | Message Board

MagicBag Grow Bags
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder

Jump to first unread post Pages: < First | < Back | 739 | 740 | 741 | 742 | 743 | 744 | 745 | 746 | 747 | 748 | 749 | 750 | 751 | 752 | 753 | 754 | 755 | 756 | 757 | 758 | 759 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Cultivation General Discussion [Re: van hatton]
    #24987909 - 02/12/18 12:59 AM (5 years, 11 months ago)

Looks good, man! I noticed you put your fingers over the lid right before unscrewing. After that, you unscrewed it the way I do it. I avoid putting my hands over the lid until I have to. Also when I grab bottles, I try and wipe my hand off on the dangling towels if I can. Overall, very good. I'm about to pour some LI into a few jars right now, maybe I'll record it.


Extras: Filter Print Post Top
Invisiblevan hattonFacebookDiscord
Still a noob
 User Gallery


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
Trusted Cultivator
Re: Cultivation General Discussion [Re: stareatclouds]
    #24987918 - 02/12/18 01:06 AM (5 years, 11 months ago)

Do it.  Well see how these jars turn out. They have some agar in the bottom.  The results from the last were..  Pretty bad I can only imagine  if that got into an lc.

Yeah with the first jar I was going to take it off like usual but I caught myself :lol: I honestly probably did it with the "master" as well. 

I'll probably  make up some more agar jars.  It's a little difficult to grab the lids like you with polyfil while keeping them sterile side down.

:cheers:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Cultivation General Discussion [Re: van hatton]
    #24987958 - 02/12/18 01:43 AM (5 years, 11 months ago)

It's just a bit of practice to be able to grab them like that. I used a lid like yours recently and it turned out fine. As long as your master of air is wiped down and was sterilized with the agar, I think these will turn out well.


Extras: Filter Print Post Top
OfflineJeebo
Stranger
Registered: 06/06/17
Posts: 71
Last seen: 5 years, 7 months
Re: Cultivation General Discussion [Re: stareatclouds]
    #24988699 - 02/12/18 12:40 PM (5 years, 11 months ago)

Hello all, need some advice, What do you think of these Filter's are they correct type I would need for a Flow hood ?


Extras: Filter Print Post Top
OfflineDoc9151M
Mycologist
Male User Gallery


Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA Flag
Last seen: 1 year, 6 months
Trusted Cultivator
Re: Cultivation General Discussion [Re: Jeebo]
    #24988972 - 02/12/18 02:49 PM (5 years, 11 months ago)

That sounds like an excellent experiment for you TBC, let us know your results, It would be cool as fk to do everything at once.


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


Extras: Filter Print Post Top
Offlinepacmanbreed
 User Gallery

Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
Re: Cultivation General Discussion [Re: Doc9151]
    #24989114 - 02/12/18 03:37 PM (5 years, 11 months ago)

Have this pinning puck inoculated 21 days ago.
Is it advisable to store it i fridge for future clonin while im out of agar?
And is it possible that bacteria can enmeshed within the pin body?
Hoping to narrow it down for a clean cultuture.


Edited by pacmanbreed (02/12/18 03:40 PM)


Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery

Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: Cultivation General Discussion [Re: pacmanbreed]
    #24989123 - 02/12/18 03:41 PM (5 years, 11 months ago)

Pins will be clean from bacteria unless the substrate is really compromised beyond mercy


--------------------
Cakes inside Water Tub


Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 14 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: pacmanbreed] * 1
    #24989127 - 02/12/18 03:43 PM (5 years, 11 months ago)

Any bacteria in your clone will likely be from the cloning process. I wouldn't store it in the fridge if you plan on cloning. Fridges are filthy, full of mold spores. I would sterilize a peice of cardboard in a pasty plate and clone to that, it'll colonize and then you can clean it up when you get more agar


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
Offlinepacmanbreed
 User Gallery

Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
Re: Cultivation General Discussion [Re: tryptkaloids]
    #24989200 - 02/12/18 04:00 PM (5 years, 11 months ago)

Thanks for the help guys. My spores are bacterial AF having a hard time cleaning from piggy backs.
Glad 1 out of ten pucks did pin.

Will score some agar today.
But will  it be better to clone from cardboard 1st before agar for cleaner mycelium?

Just curious since Ive once red from a fellow that he puts a pin in zip lock 1st then scrap that clean mycelium for agar.


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Cultivation General Discussion [Re: Mateja] * 1
    #24989291 - 02/12/18 04:31 PM (5 years, 11 months ago)

Quote:

Mateah said:
Pins will be clean from bacteria unless the substrate is really compromised beyond mercy




I don't think all bacteria is visible in a substrate. Either way, you should be transferring after cloning anything.

If you're dropping a pin to agar, you're almost certainly getting contaminants. All fruits are inherently dirty unless grown in-vitro, and even then it's not guaranteed. Pins aren't sterile. Their advantage is their rapid growth which can outrun contaminants on the agar. It's always best to transfer early on to isolate away from potential molds/bacteria. I believe RR suggested using an agar recipe lower in nutrients to give the mycelium an extra advantage over competing molds.


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 9 days
Trusted Cultivator
Re: Cultivation General Discussion [Re: pacmanbreed]
    #24989295 - 02/12/18 04:32 PM (5 years, 11 months ago)

Quote:

pacmanbreed said:
Have this pinning puck inoculated 21 days ago.
Is it advisable to store it i fridge for future clonin while im out of agar?
And is it possible that bacteria can enmeshed within the pin body?
Hoping to narrow it down for a clean cultuture.



A pin should outrun bacteria pretty easy on agar


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 14 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: pacmanbreed]
    #24989297 - 02/12/18 04:33 PM (5 years, 11 months ago)

Not sure what you're trying to ask but if you can clone to agar first. Cardboard should be last resort or for mailing cultures


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
Invisiblevan hattonFacebookDiscord
Still a noob
 User Gallery


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
Trusted Cultivator
Re: Cultivation General Discussion [Re: tryptkaloids]
    #24989460 - 02/12/18 05:47 PM (5 years, 11 months ago)

Results from the video before last?.

Not the stare style ones.



No wonder.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Cultivation General Discussion [Re: van hatton]
    #24989508 - 02/12/18 06:05 PM (5 years, 11 months ago)

Do you have any lids with SFDs on them? Or any jars as controls where you don't open them at all?


Extras: Filter Print Post Top
Invisiblevan hattonFacebookDiscord
Still a noob
 User Gallery


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
Trusted Cultivator
Re: Cultivation General Discussion [Re: stareatclouds]
    #24989560 - 02/12/18 06:28 PM (5 years, 11 months ago)

No sfds.  But I do have controls.  They are all good.  :thumbup:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery

Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: Cultivation General Discussion [Re: stareatclouds]
    #24989619 - 02/12/18 06:52 PM (5 years, 11 months ago)

Quote:

stareatclouds said:
Quote:

Mateah said:
Pins will be clean from bacteria unless the substrate is really compromised beyond mercy




I don't think all bacteria is visible in a substrate. Either way, you should be transferring after cloning anything.

If you're dropping a pin to agar, you're almost certainly getting contaminants. All fruits are inherently dirty unless grown in-vitro, and even then it's not guaranteed. Pins aren't sterile. Their advantage is their rapid growth which can outrun contaminants on the agar. It's always best to transfer early on to isolate away from potential molds/bacteria. I believe RR suggested using an agar recipe lower in nutrients to give the mycelium an extra advantage over competing molds.



I´m aware that there is always a possibility of bacteria or mold with spore solution,
but if there is nothing obvious in the jar then that invitro pin should be clean as hell, right?


--------------------
Cakes inside Water Tub


Extras: Filter Print Post Top
Offlinemadhatter2
StrangerThings

Registered: 04/22/13
Posts: 78
Last seen: 3 years, 5 months
Re: Cultivation General Discussion [Re: Mateja]
    #24989739 - 02/12/18 07:35 PM (5 years, 11 months ago)

Been reading Comebackkid's "proper surface moister" and I'm fairly confident in my monotub.  Pins are popping in all different corners.  I'm excited for that.  No real even pin set, but any feedback would be appreciated.


One more question, I've read MS may not flush.  I hear a lot of people using the term MS, meaning multispore, right?  So how would you describe what I did which is to go from spore syringe, make 6 transfers, then to grain, to bulk.  Does MS still describe what I have?  I'm sure I didn't isolate.

Edit: transfers on agar


Edited by madhatter2 (02/12/18 07:42 PM)


Extras: Filter Print Post Top
OfflineFishLevelMidnight
Aquaman
Male User Gallery


Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
Re: Cultivation General Discussion [Re: madhatter2]
    #24989758 - 02/12/18 07:42 PM (5 years, 11 months ago)

You still have MS it’s just reduced genetics.


--------------------





Trade List





Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Cultivation General Discussion [Re: van hatton]
    #24989762 - 02/12/18 07:43 PM (5 years, 11 months ago)

Quote:

van hatton said:
No sfds.  But I do have controls.  They are all good.  :thumbup:




You should lift the jar up with the Poly and see if fucking with that is letting spores through. If it's not that, I definitely think it's the force you removed and set your lids back on.

Mateah,

An in-vitro pin should be clean, but bacteria in a jar isn't always obvious. And taking the pin from in-vitro to agar is exposing it regardless meaning it's not 100% guaranteed clean. Plenty of people drop in-vitro pins to grain or LC with good results, though, so it's not like it can't be done successfully.

Quote:

One more question, I've read MS may not flush.  I hear a lot of people using the term MS, meaning multispore, right?  So how would you describe what I did which is to go from spore syringe, make 6 transfers, then to grain, to bulk.  Does MS still describe what I have?  I'm sure I didn't isolate.




You're still MS, but less strains than you initially started. And who says MS may not flush? That's like one of the pluses of MS, there's so many strains present from stable varieties that you're basically guaranteed fruits.

An unproven isolate may not fruit.


Extras: Filter Print Post Top
Offlinemadhatter2
StrangerThings

Registered: 04/22/13
Posts: 78
Last seen: 3 years, 5 months
Re: Cultivation General Discussion [Re: stareatclouds]
    #24989803 - 02/12/18 08:00 PM (5 years, 11 months ago)

Thanks fishermansjc.  This is my first monotub, first grow actually... I'm excited to see how this goes.  I guess I should be happy with this. 

I know their are so many variables, so answering hypotheticals is difficult, but...

If conditions like clean spawn, clean substrate are good:  Can a perfectly dialed in mono, or having great surface conditions allow for a even pin set using MS?


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 739 | 740 | 741 | 742 | 743 | 744 | 745 | 746 | 747 | 748 | 749 | 750 | 751 | 752 | 753 | 754 | 755 | 756 | 757 | 758 | 759 | Next > | Last >

Shop: Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder


Similar ThreadsPosterViewsRepliesLast post
* alt.drugs.mushrooms.cultivation (good/bad idea) BikeCourier 6,440 9 10/26/21 06:04 PM
by LogicaL Chaos
* Vegetative growth VS generative growth? LoveLightPeace 1,542 1 06/04/18 02:15 PM
by jason9086
* casing from "The mushroom Cultivator" upupup 17,893 8 11/13/20 10:41 AM
by tiptrippy
* YoshiTrainer's guide to low-prep grains
( 1 2 3 4 ... 12 13 all )
YoshiTrainer 8,238 249 01/07/24 06:48 PM
by MoJim
* Important - All Cultivators please read.
( 1 2 3 4 5 6 all )
ThorA 41,587 103 05/31/05 03:35 AM
by Anno
* Post deleted by users_request
( 1 2 3 4 5 all )
McMan 25,295 93 05/06/01 01:56 PM
by 3DSHROOM
* Post deleted by users_request
( 1 2 3 4 5 all )
NuShroomPharmerII 26,676 85 06/04/01 04:53 PM
by MNmyc
* VOTE!! Outdoor Cultivation Forum w/Moe For Mod!!
( 1 2 3 4 all )
TM 23,449 73 04/13/10 01:30 AM
by jingus

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,194,949 topic views. 19 members, 188 guests and 55 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.037 seconds spending 0.012 seconds on 13 queries.