|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
|
Re: Cultivation General Discussion [Re: van hatton]
#24987909 - 02/12/18 12:59 AM (5 years, 11 months ago) |
|
|
Looks good, man! I noticed you put your fingers over the lid right before unscrewing. After that, you unscrewed it the way I do it. I avoid putting my hands over the lid until I have to. Also when I grab bottles, I try and wipe my hand off on the dangling towels if I can. Overall, very good. I'm about to pour some LI into a few jars right now, maybe I'll record it.
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
|
|
Do it. Well see how these jars turn out. They have some agar in the bottom. The results from the last were.. Pretty bad I can only imagine if that got into an lc.
Yeah with the first jar I was going to take it off like usual but I caught myself I honestly probably did it with the "master" as well.
I'll probably make up some more agar jars. It's a little difficult to grab the lids like you with polyfil while keeping them sterile side down.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
|
Re: Cultivation General Discussion [Re: van hatton]
#24987958 - 02/12/18 01:43 AM (5 years, 11 months ago) |
|
|
It's just a bit of practice to be able to grab them like that. I used a lid like yours recently and it turned out fine. As long as your master of air is wiped down and was sterilized with the agar, I think these will turn out well.
|
Jeebo
Stranger
Registered: 06/06/17
Posts: 71
Last seen: 5 years, 7 months
|
|
Hello all, need some advice, What do you think of these Filter's are they correct type I would need for a Flow hood ?
|
Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 6 months
|
Re: Cultivation General Discussion [Re: Jeebo]
#24988972 - 02/12/18 02:49 PM (5 years, 11 months ago) |
|
|
That sounds like an excellent experiment for you TBC, let us know your results, It would be cool as fk to do everything at once.
--------------------
  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
|
pacmanbreed


Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
|
Re: Cultivation General Discussion [Re: Doc9151]
#24989114 - 02/12/18 03:37 PM (5 years, 11 months ago) |
|
|
Have this pinning puck inoculated 21 days ago. Is it advisable to store it i fridge for future clonin while im out of agar? And is it possible that bacteria can enmeshed within the pin body? Hoping to narrow it down for a clean cultuture.
Edited by pacmanbreed (02/12/18 03:40 PM)
|
Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
|
Re: Cultivation General Discussion [Re: pacmanbreed]
#24989123 - 02/12/18 03:41 PM (5 years, 11 months ago) |
|
|
Pins will be clean from bacteria unless the substrate is really compromised beyond mercy
-------------------- Cakes inside Water Tub
|
tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 14 hours
|
Re: Cultivation General Discussion [Re: pacmanbreed] 1
#24989127 - 02/12/18 03:43 PM (5 years, 11 months ago) |
|
|
Any bacteria in your clone will likely be from the cloning process. I wouldn't store it in the fridge if you plan on cloning. Fridges are filthy, full of mold spores. I would sterilize a peice of cardboard in a pasty plate and clone to that, it'll colonize and then you can clean it up when you get more agar
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
|
pacmanbreed


Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
|
Re: Cultivation General Discussion [Re: tryptkaloids]
#24989200 - 02/12/18 04:00 PM (5 years, 11 months ago) |
|
|
Thanks for the help guys. My spores are bacterial AF having a hard time cleaning from piggy backs. Glad 1 out of ten pucks did pin.
Will score some agar today. But will it be better to clone from cardboard 1st before agar for cleaner mycelium?
Just curious since Ive once red from a fellow that he puts a pin in zip lock 1st then scrap that clean mycelium for agar.
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
|
Re: Cultivation General Discussion [Re: Mateja] 1
#24989291 - 02/12/18 04:31 PM (5 years, 11 months ago) |
|
|
Quote:
Mateah said: Pins will be clean from bacteria unless the substrate is really compromised beyond mercy
I don't think all bacteria is visible in a substrate. Either way, you should be transferring after cloning anything.
If you're dropping a pin to agar, you're almost certainly getting contaminants. All fruits are inherently dirty unless grown in-vitro, and even then it's not guaranteed. Pins aren't sterile. Their advantage is their rapid growth which can outrun contaminants on the agar. It's always best to transfer early on to isolate away from potential molds/bacteria. I believe RR suggested using an agar recipe lower in nutrients to give the mycelium an extra advantage over competing molds.
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Cultivation General Discussion [Re: pacmanbreed]
#24989295 - 02/12/18 04:32 PM (5 years, 11 months ago) |
|
|
Quote:
pacmanbreed said: Have this pinning puck inoculated 21 days ago. Is it advisable to store it i fridge for future clonin while im out of agar? And is it possible that bacteria can enmeshed within the pin body? Hoping to narrow it down for a clean cultuture.
A pin should outrun bacteria pretty easy on agar
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 14 hours
|
Re: Cultivation General Discussion [Re: pacmanbreed]
#24989297 - 02/12/18 04:33 PM (5 years, 11 months ago) |
|
|
Not sure what you're trying to ask but if you can clone to agar first. Cardboard should be last resort or for mailing cultures
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
|
Re: Cultivation General Discussion [Re: tryptkaloids]
#24989460 - 02/12/18 05:47 PM (5 years, 11 months ago) |
|
|
Results from the video before last?.
Not the stare style ones.

No wonder.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
|
Re: Cultivation General Discussion [Re: van hatton]
#24989508 - 02/12/18 06:05 PM (5 years, 11 months ago) |
|
|
Do you have any lids with SFDs on them? Or any jars as controls where you don't open them at all?
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
|
|
No sfds. But I do have controls. They are all good.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
|
|
Quote:
stareatclouds said:
Quote:
Mateah said: Pins will be clean from bacteria unless the substrate is really compromised beyond mercy
I don't think all bacteria is visible in a substrate. Either way, you should be transferring after cloning anything.
If you're dropping a pin to agar, you're almost certainly getting contaminants. All fruits are inherently dirty unless grown in-vitro, and even then it's not guaranteed. Pins aren't sterile. Their advantage is their rapid growth which can outrun contaminants on the agar. It's always best to transfer early on to isolate away from potential molds/bacteria. I believe RR suggested using an agar recipe lower in nutrients to give the mycelium an extra advantage over competing molds.
I´m aware that there is always a possibility of bacteria or mold with spore solution, but if there is nothing obvious in the jar then that invitro pin should be clean as hell, right?
-------------------- Cakes inside Water Tub
|
madhatter2
StrangerThings

Registered: 04/22/13
Posts: 78
Last seen: 3 years, 5 months
|
Re: Cultivation General Discussion [Re: Mateja]
#24989739 - 02/12/18 07:35 PM (5 years, 11 months ago) |
|
|
Been reading Comebackkid's "proper surface moister" and I'm fairly confident in my monotub. Pins are popping in all different corners. I'm excited for that. No real even pin set, but any feedback would be appreciated.

One more question, I've read MS may not flush. I hear a lot of people using the term MS, meaning multispore, right? So how would you describe what I did which is to go from spore syringe, make 6 transfers, then to grain, to bulk. Does MS still describe what I have? I'm sure I didn't isolate.
Edit: transfers on agar
Edited by madhatter2 (02/12/18 07:42 PM)
|
FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
|
Re: Cultivation General Discussion [Re: madhatter2]
#24989758 - 02/12/18 07:42 PM (5 years, 11 months ago) |
|
|
You still have MS it’s just reduced genetics.
--------------------
 
 Trade List
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
|
Re: Cultivation General Discussion [Re: van hatton]
#24989762 - 02/12/18 07:43 PM (5 years, 11 months ago) |
|
|
Quote:
van hatton said: No sfds. But I do have controls. They are all good. 
You should lift the jar up with the Poly and see if fucking with that is letting spores through. If it's not that, I definitely think it's the force you removed and set your lids back on.
Mateah,
An in-vitro pin should be clean, but bacteria in a jar isn't always obvious. And taking the pin from in-vitro to agar is exposing it regardless meaning it's not 100% guaranteed clean. Plenty of people drop in-vitro pins to grain or LC with good results, though, so it's not like it can't be done successfully.
Quote:
One more question, I've read MS may not flush. I hear a lot of people using the term MS, meaning multispore, right? So how would you describe what I did which is to go from spore syringe, make 6 transfers, then to grain, to bulk. Does MS still describe what I have? I'm sure I didn't isolate.
You're still MS, but less strains than you initially started. And who says MS may not flush? That's like one of the pluses of MS, there's so many strains present from stable varieties that you're basically guaranteed fruits.
An unproven isolate may not fruit.
|
madhatter2
StrangerThings

Registered: 04/22/13
Posts: 78
Last seen: 3 years, 5 months
|
|
Thanks fishermansjc. This is my first monotub, first grow actually... I'm excited to see how this goes. I guess I should be happy with this.
I know their are so many variables, so answering hypotheticals is difficult, but...
If conditions like clean spawn, clean substrate are good: Can a perfectly dialed in mono, or having great surface conditions allow for a even pin set using MS?
|
|