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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
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Re: Cultivation General Discussion [Re: Ferather]
#24963977 - 02/03/18 01:06 PM (5 years, 11 months ago) |
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Question for anyone that's used a culture after refrigeration. Since the cultures growth was inhibited during refrigeration would there be a period of time it would take to gain it's vigor again if it were used to inoculate grains?
-------------------- Keep your feet on the ground and your head in the clouds
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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I can put mycelium into the fridge, take a sample cold, put it into room temp whatever and get a response after 8-12 hours.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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I would let it grow out on a new plate first but then it should not be set back
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24963992 - 02/03/18 01:12 PM (5 years, 11 months ago) |
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Might depend on the mycelium and the media also, try both, no harm will be done.
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24964002 - 02/03/18 01:16 PM (5 years, 11 months ago) |
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Cool thanks guys. Was just a random thought, but good to know for the future. If a plate was grown out uniform and kept in a fridge dedicate to myco work do you think it would be fine to use without taking a transfer and growing that out?
-------------------- Keep your feet on the ground and your head in the clouds
Edited by Shineonyoucrazy (02/03/18 01:19 PM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Well, I have never heard of death due to: low temps to rooms temps, even when cut. I know you can kill some mycelium with lower temps, such as Pink oyster.
Pink oyster mycelium will die if the temp goes below 10-12°C.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
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Re: Cultivation General Discussion [Re: Ferather] 1
#24964012 - 02/03/18 01:22 PM (5 years, 11 months ago) |
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I've noticed no significant differences with leap off from refrigerated cultures and ones still sitting in my incubation area. I usually let it set out a few hours while I make agar or while grain jars cool.
Sometimes I take a transfer and let it grow out for use, sometimes I just use the culture. Just depends on what I've got sittin around. I have enough atl7 cultures to just use one straight from cold storage, but most people only store masters.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: 00Burnout]
#24964026 - 02/03/18 01:29 PM (5 years, 11 months ago) |
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I agree, I don't think it's a cause for concern for mycelium that can handle the temperatures, just be clean.
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Xerbia
Stranger

Registered: 02/24/10
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Re: Cultivation General Discussion [Re: Ferather]
#24964226 - 02/03/18 03:30 PM (5 years, 11 months ago) |
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New Oyster fruits already starting to pop up. They're all looking much healthier this time, especially the blues!
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
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Re: Cultivation General Discussion [Re: Xerbia]
#24964232 - 02/03/18 03:34 PM (5 years, 11 months ago) |
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Those are beautiful! I wonder how well they will keep their color as they mature. Mine usually start out like that but most of the color is lost as they mature.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: 00Burnout]
#24964851 - 02/03/18 04:55 PM (5 years, 11 months ago) |
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Wow gorgeous.
Could you provide details of the environment and substrate.?
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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madhatter2
StrangerThings

Registered: 04/22/13
Posts: 78
Last seen: 3 years, 5 months
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Re: Cultivation General Discussion [Re: 00Burnout]
#24964955 - 02/03/18 05:18 PM (5 years, 11 months ago) |
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Dilemma! Clear pictures through mono aren't very clear, so hopefully a description will suffice. I'm using images from Spitball's "Spawing to Bulk and Fruiting" as a reference. It's day 11 and have not removed the lid of my mono. I'm running a 1:4 rye on cvg in a 66g mono. The grain at the surface of the substrate is nice and white, but that is where the fluffy white mycelium stops, everywhere else is a thin wisp of mycelium. So thin you can still see the cvg beneath. Has anyone fruited with a monotub of this description with success? https://www.shroomery.org/forums/showflat.php/Number/20470129/fpart/1/vc/1 Mine looks like Spit's day 3, kind of, but fully covered and thinner.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: madhatter2]
#24965002 - 02/03/18 05:40 PM (5 years, 11 months ago) |
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Where have you read you can't take the lid off to take a pic? You can write a wall of text to describe your tub and you won't be able to convey 1% of what an image can tell us.
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madhatter2
StrangerThings

Registered: 04/22/13
Posts: 78
Last seen: 3 years, 5 months
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Re: Cultivation General Discussion [Re: Josex]
#24965027 - 02/03/18 05:56 PM (5 years, 11 months ago) |
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Quote:
Josex said: Where have you read you can't take the lid off to take a pic? You can write a wall of text to describe your tub and you won't be able to convey 1% of what an image can tell us.
I appreciate the idea that a description is basically pointless.
Opening the lid and allowing the Co2 to escape was my worry.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: madhatter2] 1
#24965037 - 02/03/18 06:00 PM (5 years, 11 months ago) |
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i can pull canopies without a lid. your concern is unjustified imo
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RuxBux
WhiskeyTimeMachine


Registered: 01/04/18
Posts: 260
Loc: Tundra Wasteland
Last seen: 2 years, 27 days
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Re: Cultivation General Discussion [Re: madhatter2]
#24965044 - 02/03/18 06:04 PM (5 years, 11 months ago) |
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Quote:
madhatter2 said:
Quote:
Josex said: Where have you read you can't take the lid off to take a pic? You can write a wall of text to describe your tub and you won't be able to convey 1% of what an image can tell us.
I appreciate the idea that a description is basically pointless.
Opening the lid and allowing the Co2 to escape was my worry.
Edited by RuxBux (02/03/18 06:05 PM)
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madhatter2
StrangerThings

Registered: 04/22/13
Posts: 78
Last seen: 3 years, 5 months
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Re: Cultivation General Discussion [Re: RuxBux]
#24965054 - 02/03/18 06:08 PM (5 years, 11 months ago) |
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Quote:
RuxBux said:
Quote:
madhatter2 said:
Quote:
Josex said: Where have you read you can't take the lid off to take a pic? You can write a wall of text to describe your tub and you won't be able to convey 1% of what an image can tell us.
I appreciate the idea that a description is basically pointless.
Opening the lid and allowing the Co2 to escape was my worry.

And by that I mean, a picture is better. MUCH better
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: madhatter2]
#24965072 - 02/03/18 06:13 PM (5 years, 11 months ago) |
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An image is not only much better, it's what you have to do if you want people to give you any kind of answer. A description is just useless no matter how detailed it is. I don't know what you're reading but that whole CO2 theory is ridiculous and from the stone age, you need to read current info. Go ahead and take a pic, open the lid for as long as you need, no rush, everything will be fine. I open the lids of my tubs lots of times while they colonize to see the surface.
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madhatter2
StrangerThings

Registered: 04/22/13
Posts: 78
Last seen: 3 years, 5 months
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Re: Cultivation General Discussion [Re: Josex]
#24965080 - 02/03/18 06:15 PM (5 years, 11 months ago) |
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Quote:
Josex said: An image is not only much better, it's what you have to do if you want people to give you any kind of answer. A description is just useless no matter how detailed it is. I don't know what you're reading but that whole CO2 theory is ridiculous and from the stone age, you need to read current info. Go ahead and take a pic, open the lid for as long as you need, no rush, everything will be fine. I open the lids of my tubs lots of times while they colonize to see the surface.
Got it!
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pacmanbreed


Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
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Re: Cultivation General Discussion [Re: Ferather]
#24965185 - 02/03/18 06:59 PM (5 years, 11 months ago) |
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Quote:
Ferather said: Here is a light explanation:
Lignicolous mycelium use laccase enzymes, which require O2 to properly decay phenol's-other. Cubensis does not use laccase, and it's requirements for oxygen to feed is lower.
In short, Cubensis does not oxidize as much as Oysters do to feed.
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Some of my active materials theories here, I believe there may be an overlooked oxidization factor.
It may be possible that the external mushroom (what we see) looks normal in low O2. However, internally (we cannot see), it may want more oxygen for materials.
Has anyone managed to side-by-side high O2 vs low O2 potency?
 Good explanation ferather. They even use this bio mimicry in paper industries to degrade lingin then alakali-acid hydrolisis to purify cellulose. O2 & CO2 has a part in mycelial building blocks. Incase of cubes, Case & fruit at spawning. Let them do their thing.
Edited by pacmanbreed (02/03/18 07:16 PM)
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