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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Changa Alchemist]
    #24959859 - 02/01/18 02:50 PM (5 years, 11 months ago)

I wouldn't say the area you're talking about is necesarily bacterial but it isn't worth a transfer for sure


--------------------
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InvisibleChanga Alchemist
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24959918 - 02/01/18 03:15 PM (5 years, 11 months ago)

No. Most definitely not. That was never in question. Just the clear film


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Offline00Burnout
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24960019 - 02/01/18 04:00 PM (5 years, 11 months ago)

Quote:

tryptkaloids said:
I wouldn't say the area you're talking about is necesarily bacterial but it isn't worth a transfer for sure




Probably would've known if it was left longer. When my plates look like that, and I believe in my case bacteria, it jumps back up further out. I've also seen mycelium "jump" over bacteria.

There's been a few cases when it gets thin like that and just keeps getting thinner, which I assume in that case it's genetics.

I'm definitely no expert at spotting bacteria, I just kinda know how to distinguish good growth from bad growth. With my oysters I can't really tell if it's bacteria or just the mycelium.


--------------------
Peace, pot and microdot!:mushroom2:
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Invisiblevan hattonFacebookDiscord
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24960049 - 02/01/18 04:19 PM (5 years, 11 months ago)

Oysters don't give a fuck about bacteria.

Don't trouble shoot bacteria problems with oysters they don't care.

Bacteria jar





This guy is 36 hours post shake.  I got high hopes for him.



I think my problem ATM KS technique.

Need to take a video of me pouring lc into jars.

Filled a 4oz jelly fish with agar and used a grain lid.



Just from lifting like I do with grain some bacteria.

So I'll work on that.  Just felt like sharing.


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
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Offline00Burnout
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Re: Cultivation General Discussion [Re: van hatton]
    #24960071 - 02/01/18 04:33 PM (5 years, 11 months ago)

The only reason I worry is for trade purposes. I've stated in my trade list that I'm not certain of oyster culture cleanliness, but I'm still not completely comfortable with sending out cultures that aren't 100% clean. That's why I have prints for trade too (because we all love hunting through ms for that perfect clone)


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Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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OfflineFerather
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Re: Cultivation General Discussion [Re: cronicr]
    #24960076 - 02/01/18 04:37 PM (5 years, 11 months ago)

Quote:

lewp said:
Are there any good posts/guides that discuss substrate and spawn pH? I'm working with some different species and want to start monitoring and adjusting pH of my mediums.




Quote:

cronicr said:
Probably in the gourmet forum, not something most pay attention to.




:whathesaid:

Are you talking Cubensis, or Gourmet? If Cubensis, mostly ignore it unless you want optimum.
Grains should be good as they are, unless the water added is acidic, coir the same.

Coir should be pre-treated to grow things, such as coir bedding (TC data).

Actual answer: pH 7 for spawn, pH 7 for the substrate.

----

Else:

You will need to know what enzymes your mycelium uses-going to use, and what pH they work best at.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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Edited by Ferather (02/01/18 04:52 PM)


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OfflineThufir_Hawat
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Re: Cultivation General Discussion [Re: Ferather]
    #24960266 - 02/01/18 06:25 PM (5 years, 11 months ago)

Excited for cultivation work this weekend! Just got an LGT print from a wonderful poster, and should have an AA+ syringe arriving in the next couple of days.

The LGT spores are going straight to MEA plates, but I'm thinking of preparing a couple of preparing a couple of half full quart jars (WBS+coir? Straight WBS?) to squirt the AA+ spores on and try for some in-vitro fruits to clone from.

Also going to use the last of the reishi LC I've had in the fridge on a single quart of WBS which hopefully will colonize quickly enough that I can break it up to do a tub grow. Both of my jars of straight WBS predictably failed to get beyond the beginning stages of fruit formation, it wants wood to eat!


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OfflineShineonyoucrazy
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Re: Cultivation General Discussion [Re: Thufir_Hawat]
    #24960404 - 02/01/18 07:15 PM (5 years, 11 months ago)

So when do you say fuck it on a tub that still hasn't knotted or fruited? I have a 32 qt unmoded tub that got started the beginning of January, and still no sign of fruiting.  It doesn't smell bad, no metabolites, no other red flags besides it just not wanting to do anything.  Any suggestions or should it just get trashed. ?


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Keep your feet on the ground and your head in the clouds :mushroom2:


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InvisibleChanga Alchemist
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Re: Cultivation General Discussion [Re: Shineonyoucrazy]
    #24960412 - 02/01/18 07:18 PM (5 years, 11 months ago)

What are you growing? Can you show us a picture? It started colonizing at the beginning of january, or you put into fruiting then?


--------------------
:ganja::mushroom::mushroom::flyhigh:
:sporedrop:~~~:peyote::sanpedro:~~~:sporedrop:

:chugbeer:


Edited by Shomann (02/01/18 07:19 PM)


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: Shineonyoucrazy]
    #24960414 - 02/01/18 07:18 PM (5 years, 11 months ago)

Move on.  From multispore sometimes and unfruiting strain will culminate.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleChanga Alchemist
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Re: Cultivation General Discussion [Re: hamloaf]
    #24960421 - 02/01/18 07:20 PM (5 years, 11 months ago)

Ham, wouldn't the odds of isolating too much and ending up with a culture that doesn't fruit be much higher than MS? Does this actually happen with MS? I personally haven't experienced it.


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:ganja::mushroom::mushroom::flyhigh:
:sporedrop:~~~:peyote::sanpedro:~~~:sporedrop:

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OfflineShineonyoucrazy
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Re: Cultivation General Discussion [Re: Changa Alchemist]
    #24960455 - 02/01/18 07:28 PM (5 years, 11 months ago)

it's ms>agar>rye>coir verm it pretty much seems like it's shit the bed. I just started some new jars last night from agar to blenderless li, but didn't know if I should just let this ride till I need the tub.
Edit this was the pasty plate dropped in for blenderless li


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


Edited by Shineonyoucrazy (02/01/18 07:48 PM)


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Invisiblestareatclouds
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Re: Cultivation General Discussion [Re: Shineonyoucrazy]
    #24961074 - 02/02/18 02:22 AM (5 years, 11 months ago)

What's the coldest fridge temp a plate can survive? I finally got a little cooler with ice packs in it and my plate was at like 31F when I checked last. Would a little warmer would be optimal?


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Offline00Burnout
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Re: Cultivation General Discussion [Re: stareatclouds]
    #24961078 - 02/02/18 02:25 AM (5 years, 11 months ago)

I'd shoot for a little warmer, you don't want it to freeze. I froze a plate once and somehow it survived.


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Peace, pot and microdot!:mushroom2:
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Invisiblestareatclouds
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24961079 - 02/02/18 02:27 AM (5 years, 11 months ago)

Yeah, that's what I always figured, something like 40F. But Googling showed me a mix of results so I figure I'll ask here for something currently acceptable.


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Offline00Burnout
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Re: Cultivation General Discussion [Re: stareatclouds]
    #24961197 - 02/02/18 05:15 AM (5 years, 11 months ago)

Sounds about right. Were your ice packs frozen? I just use ice packs as a thermal barrier so they're never colder than the coldest my fridge gets.

I really need a mini fridge, my little lunchbox is nearly full and I still have a dozen cultures that will need to go in cold storage soon.

A thought just occured to me. Do we need to worry about ecological impact when starting beds for species not native to our area? I know some plants can and have become problems due to negligent gardeners and foolish introduction. Can growing foreign species have negative impacts on our ecosystem?

Considering that microorganisms are the reason the rest of life can exist, I could see introducing a new species being detrimental. I could be worrying about nothing, and maybe our microbiotic ecosystems are more stable than I think.


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Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24961333 - 02/02/18 07:17 AM (5 years, 11 months ago)

Quote:

and maybe our microbiotic ecosystems are more stable than I think




This part. Anywhere fungus grows there is a whole plethora of bugs to eat fungus, not to mention competing and parasitic microorganisms.
I would only worry about plants and animals when it comes to invasive species.. Microorganisms have insanely similar genetics from the deepest valley to the highest mountain the soil microbes don't get enough radiation to mutate very fast so the microbial kingdom in the soil is insanely stable


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Invisibleforagedfungus
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24961358 - 02/02/18 07:42 AM (5 years, 11 months ago)

Non-native fungi can and will escape cultivation.
I've read about shiitake and yellow oysters being found in the woods in the USA. Neither one is native to north america.
The spores are spread from mushroom farms and they become naturalized.

I'm not sure what impact this has on native ecosystems. Some non-native species destroy ecosystems, others blend right in/even help (apples aren't native to N. america, they are everywhere now, and I've never heard anyone complain they are invasive)


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Offline00Burnout
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24961363 - 02/02/18 07:48 AM (5 years, 11 months ago)

Sweet.

Well I'm going to start a cube patch this spring and hopefully a ps. alutaceae patch this fall. I know the cubes won't survive next winter but the alutaceae patch could, I doubt they will make much of an impact. I've got wonderful spots picked out. My girl is cool with outdoor grows, she's slowly coming around :awedance:


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Invisiblestareatclouds
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24961557 - 02/02/18 10:20 AM (5 years, 11 months ago)

Quote:

00Burnout said:
Sounds about right. Were your ice packs frozen? I just use ice packs as a thermal barrier so they're never colder than the coldest my fridge gets.




Nah, not frozen. Just for insulation and to keep the cultures stable from the freezer cycles.


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