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InvisibleAyePlusS
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Registered: 12/18/14
Posts: 3,393
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Re: Cultivation General Discussion [Re: Just_A_Noob] * 1
    #24935020 - 01/22/18 09:34 PM (6 years, 8 days ago)

Quote:

Just_A_Noob said:
Quote:

Germs said:
Quote:

hamloaf said:
I'm sober as a jay bird.  :awesomenod:



That’s a new one, gonna write that down

Do any birds eat mushrooms?







I shoulda never gave em a taste.  They rip up the cyan patch any chance they get



RIP my backyard allenii patch. Asshole chickens.


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Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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OfflineMarmie
Peter peter portion eater
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Re: Cultivation General Discussion [Re: AyePlus] * 1
    #24935032 - 01/22/18 09:40 PM (6 years, 8 days ago)

The mycelium will just take what it needs, gypsum is said to help with fruit formation,  but gypsum is a pH buffer so it also helps with stopping pH swings in later flushes as the myceliom consumes the substrate.


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Offlinepacmanbreed
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Re: Cultivation General Discussion [Re: Marmie]
    #24935176 - 01/22/18 11:26 PM (6 years, 8 days ago)

Agreed. :cookiemonster:

Yes gypsum is a good slow acting buffer for some grains @ 3% if grains have unbalnce amount of calcium and sulfate. But too much it will just be wasted..

Gypsum is ph stable Just like ferather have said some grains have short lived ph for a-amylase (6.7-7.00 ph)
Usefull for buffering @3%. Thats why its also used in  brewing water.

In terms of grain agar ive been noticing a good growth buffering the ph to 7-7.5ph by adding a pinch of powdered eggshell about 1 eggshell or 10-15g per 700ml water for a shortterm fast acting buffer specially if tea is added.

This is what i do for agar almost mimicking a ruminant digestive path.
- boil water.
- steep tea
- add brf
- let the nute hydrolyze & react to the acidic water
- bottle while hot
- add caco3
- sit for aday and let the caco3 slowly react to the 4.5-5.5 ph
- measure increased ph next day.
- cook agar if it reached ph near 7.

Thank for posting the enzyme optimal ph brother.
https://www.shroomery.org/forums/showflat.php/Number/24847183#24847183.

I think as for protien they produce peptidase with an optimal ph of 4.7 to 6.25


Edited by pacmanbreed (01/22/18 11:33 PM)


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OfflineMarmie
Peter peter portion eater
I'm a teapot


Registered: 10/21/15
Posts: 918
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Re: Cultivation General Discussion [Re: pacmanbreed]
    #24935236 - 01/23/18 12:38 AM (6 years, 7 days ago)

I just made 50 mea slants and they all have some caramelization in them, will they still be fine to use or should i scrap em n try again tomarrow?


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
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Re: Cultivation General Discussion [Re: Marmie]
    #24935358 - 01/23/18 03:43 AM (6 years, 7 days ago)

Use em, I doubt it's actual carmelization


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinepacmanbreed
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Re: Cultivation General Discussion [Re: Marmie]
    #24935414 - 01/23/18 05:06 AM (6 years, 7 days ago)

:whathesaid: use em.


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InvisibleLizardWizard
GnomeGrower
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Folding@home Statistics
Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
Re: Cultivation General Discussion [Re: hamloaf]
    #24935442 - 01/23/18 05:43 AM (6 years, 7 days ago)

Do you guys start counting those 3 hours
Quote:

hamloaf said:
Consider going with 3 hours especially at 15 psi, and saying the bags are full of 3quarts of grain or more.




When do you guys start counting those 3 hours? Or 2,5 at 20? From the moment you reach pressure, or from the moment you the substrate is at temp?


--------------------
The best things in life
can be smelled on one's fingers.


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OfflineFerather
Mycological
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Registered: 03/19/15
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Re: Cultivation General Discussion [Re: pacmanbreed] * 1
    #24935450 - 01/23/18 05:49 AM (6 years, 7 days ago)

@pacmanbreed, Good stuff dude.

----

Extract from my pocket guide, with added Amylase cleavage:

----

Enzymes

Disclaimer: Optimal pH varies with the organism, and it's enzymes.


Cellulase (cellulose): 4.5-8.5.
Laccase (phenol's): 5.5-6.0.
Amylase (starch): 3.0-7.0.

Protease (protein): 2.5-11.


For cellulase there are four types:

Alkaline cellulase: 7.2-8.5.
Neutral cellulase: 6.0-8.0.
Hybrid cellulase: 4.5-7.0.
Acid cellulase: 4.5-5.0.


For amylase there are three types:

Ξ±-Amylase: 6.7-7.0. < Any amount of glucose.
Ξ²-Amylase: 4.0-5.0. < Cleaves two units.
Ξ³-Amylase: 3.0. < Cleaves one unit.


Laccase remains stable at pH 5.0-10.0.


For protease there are three types:

Alkaline protease: 7.0-11.0.
Neutral protease: 6.0-9.0.
Acid protease: 2.5-4.0.

----

There are also other nitrogen sources other than protein.
Some nitrogen sources don't need enzymes at all.

Calcium bicarbonate also needs no enzymes.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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Offlinehamloaf
Loaf of Fam.
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Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
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Re: Cultivation General Discussion [Re: LizardWizard]
    #24935461 - 01/23/18 06:01 AM (6 years, 7 days ago)

Quote:

LizardWizard said:
Do you guys start counting those 3 hours
Quote:

hamloaf said:
Consider going with 3 hours especially at 15 psi, and saying the bags are full of 3quarts of grain or more.




When do you guys start counting those 3 hours? Or 2,5 at 20? From the moment you reach pressure, or from the moment you the substrate is at temp?



The moment the desired psi's are reached.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleLizardWizard
GnomeGrower
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Re: Cultivation General Discussion [Re: hamloaf] * 1
    #24935463 - 01/23/18 06:02 AM (6 years, 7 days ago)

Thanks!

:asianofapproval:


--------------------
The best things in life
can be smelled on one's fingers.


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OfflineFerather
Mycological
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Registered: 03/19/15
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Re: Cultivation General Discussion [Re: LizardWizard] * 1
    #24935474 - 01/23/18 06:13 AM (6 years, 7 days ago)

These images you have seen before, but I will now explain further.

This is Cambodian, brown rice spawn, pH 7.5 paper, enriched for more yield. You can see visibly it is highly populated, aerial and very fast.
The added soluble macro-micro nutrients do not require enzymes, also when it switched to cellulose, it had good nutrients.

The nitrogen sources where ammoniacal and ureic nitrogen, natively present in dung, does not require protease.

This was 6 years ago, before I knew how to fruit anything correctly.

   

   

Practically gourmet style, adding nutrients.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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Offlinepacmanbreed
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Registered: 10/12/16
Posts: 3,659
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Re: Cultivation General Discussion [Re: Ferather]
    #24935716 - 01/23/18 09:23 AM (6 years, 7 days ago)

Interesting stuff ferather. Dung like papers.  :rockon:
Really dug deep on their hydrolitic process. :thumbup:

Getting better @ cubes.
they are specialized in a-amylase for carbon
In utilizing the ureic and ammonical nitrogen
To build glycogen etc
Then switch to cellulase in later stages specially when fruiting and c:n has reached its peak.

Im still diggin deeper understanding why a tea-agar or wl-substrate is so resistant even mold spore present in air if c & n is present. I guess due to the minimal amount?..
Is that asceptically inoculated or open air?

Is wl-enrichment used?
Code:
Optional: 12g Black Tea Leaves + 4-8g Calcium Carbonate (CaCO3).
Beneficial Bacteria: 2-4g Liquid Seaweed (powder may work).

4-8g, (5.00-10ml) > Miracle-Gro Soluble Nutrients.
0.5-1g, (0.62-1.24ml) > Yeast Nutrients.
100g > Dry Pellets (any type).



If they knotup in that syntetic dung. Its a nice alternative substrate to pf-pucks for further bacterial isolation.


Edited by pacmanbreed (01/23/18 09:36 AM)


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OfflineMahdi
Temporal
I'm a teapot


Registered: 12/17/16
Posts: 257
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Re: Cultivation General Discussion [Re: pacmanbreed]
    #24935957 - 01/23/18 11:28 AM (6 years, 7 days ago)

has anyone tried using sterilized qtips to swipe a print to agar instead of an inoculation loop?


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Offline00Burnout
That one guy
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Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
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Re: Cultivation General Discussion [Re: Mahdi]
    #24935971 - 01/23/18 11:33 AM (6 years, 7 days ago)

I'm sure someone has. Most of the time people swioe the gills with a qtip then a plate so I don't doubt it would work for a print. Penis varieties pretty much necessitate swiping the gills.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Invisibleeatyualive
Eat's You Alive :)
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Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24935979 - 01/23/18 11:35 AM (6 years, 7 days ago)

Quote:


has anyone tried using sterilized qtips to swipe a print to agar instead of an inoculation loop?





yes. they call it swabbing. its easy and i actually prefer it better than using a loop.

https://www.shroomery.org/forums/showflat.php/Number/22556356


--------------------
EAT GETS SHIT DONE


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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: Mahdi]
    #24936087 - 01/23/18 12:09 PM (6 years, 7 days ago)

Quote:

Mahdi said:
has anyone tried using sterilized qtips to swipe a print to agar instead of an inoculation loop?



That’s the only way I do it. I bought 100 2-packs of pre-sterilized swabs, been almost a year and I’m barely halfway into it


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InvisibleChanga Alchemist
Shwift sauce lifeguard
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Registered: 03/16/16
Posts: 2,707
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Re: Cultivation General Discussion [Re: Germs]
    #24936119 - 01/23/18 12:23 PM (6 years, 7 days ago)

Swabbing :rockon:

I cut a piece of a swab and dropped it on an agar puck with josex's tek. I really like germinating this way and transfering to agar later.


--------------------
:ganja::mushroom::mushroom::flyhigh:
:sporedrop:~~~:peyote::sanpedro:~~~:sporedrop:

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Offline00Burnout
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Re: Cultivation General Discussion [Re: Changa Alchemist]
    #24936170 - 01/23/18 12:43 PM (6 years, 7 days ago)

I thought that's what it's called but it just didn't sound right in my head...I blame lack of sleep and sobriety lol


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offlinehamloaf
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24936216 - 01/23/18 12:57 PM (6 years, 7 days ago)

Inoculation loop!  :highfive:


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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OfflineFishLevelMidnight
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Re: Cultivation General Discussion [Re: hamloaf]
    #24936229 - 01/23/18 01:02 PM (6 years, 7 days ago)

I’m in the swab game- the spores stick easily but get transferred well to the plate.
I individually wrapped swabs in foil and autoclave 15 psi for 30 min then throw in a bag.


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