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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: cronicr]
#24933327 - 01/22/18 09:29 AM (6 years, 8 days ago) |
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Are you sure? Because i disagree.
Im not going behind anyone. Wtf
Im about mushrooms not that shit.
Edited by mushboy (01/22/18 09:30 AM)
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: mushboy]
#24933328 - 01/22/18 09:29 AM (6 years, 8 days ago) |
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Anyone ever throw spent coffee grains in the water they are boiling for grain prep? I am bringing some water up to a boil for oats, and there was left over coffee grounds from the wife. I said fuck it and dumped it in the water. I don't think it can hurt a thing, I PC for 2.5hrs at about 20PSI. I want to see if it might happen to speed colonization of the grains any.
-------------------- JOIN THE POW WOW
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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It won't hurt anything most just use liquid coffee though.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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I can assemble T-Gel, plain, without pressure cooking, and open to air, and only get mold around 1/10 times. Would I suggest doing it that way, no, I would suggest PC and at least a SAB, it's better overall.
Currently I have an open hole in my lid, filter removed, wouldn't suggest that either. You could argue I don't need the PC or a SAB, based one only 1 loss.
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Quote:
TheMadHatter420 said: Anyone ever throw spent coffee grains in the water they are boiling for grain prep? I am bringing some water up to a boil for oats, and there was left over coffee grounds from the wife. I said fuck it and dumped it in the water. I don't think it can hurt a thing, I PC for 2.5hrs at about 20PSI. I want to see if it might happen to speed colonization of the grains any.
Coffee is acidic, adding it will attract mold, and reduce effectiveness of α-Amylase (pH 6.7-7.0). Try coffee + lime to get the end pH to 7, or slightly above, for better overall decay.
Coffee bean, roasted, ground, nutrient composition (contains starch).
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: Ferather]
#24933394 - 01/22/18 09:59 AM (6 years, 8 days ago) |
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How much could it effect a sterile media? Coffee and ph that is? I have never tested or entertained the thought of liming grain spawn the only tests i have seen were colonizing Times with and without liquid coffee. Adding anything pre pc will not attract mold...mold attracts mold lol
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: cronicr]
#24933407 - 01/22/18 10:04 AM (6 years, 8 days ago) |
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If you are being 100% aseptic, no real issues adding it other than pH, it's soluble like tea, so it's quick to decay. It should increase speed and decay, but lets say you made the grains pH 5.5, it would slow down.
The acidic pH would counter and destabilize the starch enzyme α-Amylase. β-Amylase (pH 4.0-5.0) is far less effective than α-Amylase.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: Ferather]
#24933414 - 01/22/18 10:06 AM (6 years, 8 days ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: cronicr]
#24933438 - 01/22/18 10:16 AM (6 years, 8 days ago) |
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You are welcome, here is some extra shiz:
Dung has a pH of 7 or more, the cellulase expelled by Cubensis I found is hybrid (pH 4.5-7.0). Ideally you end substrate should be pH 7, this also helps α-Amylase enzymes.
I'm not 100% sure about the protease, but they seem fine at pH 7.
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Advanced: It will utilize ammoniacal and ureic nitrogen.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Ferather]
#24933556 - 01/22/18 11:06 AM (6 years, 8 days ago) |
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Quote:
Ferather said: I can assemble T-Gel, plain, without pressure cooking, and open to air, and only get mold around 1/10 times. Would I suggest doing it that way, no, I would suggest PC and at least a SAB, it's better overall.
Currently I have an open hole in my lid, filter removed, wouldn't suggest that either. You could argue I don't need the PC or a SAB, based one only 1 loss.
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Quote:
TheMadHatter420 said: Anyone ever throw spent coffee grains in the water they are boiling for grain prep? I am bringing some water up to a boil for oats, and there was left over coffee grounds from the wife. I said fuck it and dumped it in the water. I don't think it can hurt a thing, I PC for 2.5hrs at about 20PSI. I want to see if it might happen to speed colonization of the grains any.
Coffee is acidic, adding it will attract mold, and reduce effectiveness of α-Amylase (pH 6.7-7.0). Try coffee + lime to get the end pH to 7, or slightly above, for better overall decay.
Coffee bean, roasted, ground, nutrient composition (contains starch).
Well I didn't see this, but did throw some gypsum in the water. It was not much spent coffe and it went into about 2 gal water, so hopefully my ph isn't off to much, specially since my tap water is 8+ph
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tealseal
spice of life ( o )( o )



Registered: 01/19/16
Posts: 678
Last seen: 6 months, 20 days
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MAD ur doing 2.5 hours for oats? i got wbs going right now in my pc and i forgot how long to let em go for, i set it for 2 hours, hope thats not too long
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: tealseal]
#24933921 - 01/22/18 01:54 PM (6 years, 8 days ago) |
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Perfect
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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tealseal
spice of life ( o )( o )



Registered: 01/19/16
Posts: 678
Last seen: 6 months, 20 days
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Re: Cultivation General Discussion [Re: cronicr]
#24933925 - 01/22/18 01:55 PM (6 years, 8 days ago) |
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shweet
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: tealseal]
#24934047 - 01/22/18 02:53 PM (6 years, 8 days ago) |
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Consider going with 3 hours especially at 15 psi, and saying the bags are full of 3quarts of grain or more.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: cronicr]
#24934065 - 01/22/18 03:01 PM (6 years, 8 days ago) |
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Quote:
cronicr said: I lift from the bottom but I got a bit of room in my sab so life's easy
My main issue is I can only get decently priced petris in packs of 25 which is too tall to stand up in my SAB. It's kind of a PITA to cut open the bag and remove them without bumping them around. I've probably only poured maybe 100-125 plates total and wound up with maybe 20 of them with green before inoculating. I blame it on maneuvering the bag around in the SAB to get the plates out.
Going to buy some 20 packs soon.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Consider splitting the stack in half, then lifting from the bottom.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: hamloaf]
#24934070 - 01/22/18 03:03 PM (6 years, 8 days ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: hamloaf]
#24934151 - 01/22/18 03:36 PM (6 years, 8 days ago) |
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Quote:
hamloaf said: Consider splitting the stack in half, then lifting from the bottom.
How I do it
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Germs] 1
#24934323 - 01/22/18 04:47 PM (6 years, 8 days ago) |
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@TheMadHatter420
Gypsum: (CaSO4), adds calcium, sulfur and oxygen, has a near neural pH, does not neutralize acids. Lime: (CaCO3), adds calcium, carbon and oxygen, has a high pH, neutralizes acids.
CaCO3 reacts with CO2 (and acids) to form soluble calcium bicarbonate.
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Forgot to mention, both Oyster and Cubensis utilize the "soluble" calcium bicarbonate.
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pH and materials:
Lets take wood as an example, for an overall better picture, lets say the wood has a pH of 5, and contains inhibitory phenol's. Cubensis is not lignicolous, it does not have laccase and cannot target the phenol's with enzymes, so its screwed.
If I added starch spawn, most of this would be overcome, and secondary decay of the phenol's can occur. However as mentioned earlier, α-Amylase is stable at pH 6.7-7.0, so again back to screwed.
Now lets change the pH via CaCO3, which will decay the acids and increase the pH. It's no longer inhibitory (or as much), and α-Amylase is now stable.
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pH and spawn:
Now lets think about fruitable life span, pH 6 vs pH 7, it would produce less yield at pH 6.
This is because mycelium make substrates more acidic over time. Thus, over time starch falls off as a carbon source.
If enzymes are unable to effectively decay the grain, it's level of carbon and nutrients drops. Coir is nutritionally weak, and the mycelium must now relay on it for nutrients.
Grain unfortunately has a short window in terms of pH, unlike cellulose.
Edited by Ferather (01/22/18 05:35 PM)
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tealseal
spice of life ( o )( o )



Registered: 01/19/16
Posts: 678
Last seen: 6 months, 20 days
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Re: Cultivation General Discussion [Re: cronicr]
#24934632 - 01/22/18 06:50 PM (6 years, 8 days ago) |
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Quote:
cronicr said: Perfect

came out looking like this, still a lil warm to the touch, hows it look?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: tealseal]
#24934636 - 01/22/18 06:51 PM (6 years, 8 days ago) |
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Nailed it
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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