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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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A random question. If I cool the inoculation loop in nutrient rich agar media before scraping off some prints, can I potentially contaminate the print if a speck of agar smears off on the print?
-------------------- Cakes inside Water Tub
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Mateja]
#24924129 - 01/18/18 07:42 PM (6 years, 12 days ago) |
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The potential for concern is there. Have yet to have that happen though.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: hamloaf]
#24924135 - 01/18/18 07:47 PM (6 years, 12 days ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: cronicr]
#24924145 - 01/18/18 07:50 PM (6 years, 12 days ago) |
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Thanks that sounds reassuring
-------------------- Cakes inside Water Tub
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Re: Cultivation General Discussion [Re: cronicr]
#24924149 - 01/18/18 07:51 PM (6 years, 12 days ago) |
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I've thought about running through the house with an open plate just to see what kind of funky shit grows, I just forget to any time I pour some plates.
Of course I'd throw it in a zip lock, maybe 2 lol.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Mateja]
#24924156 - 01/18/18 07:54 PM (6 years, 12 days ago) |
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While we are at it I have another question I´ve been pondering about..
It´s reasonable to assume that a bulk substrate that has been dunked will have a few pockets of nutrient rich droplets of standing water, deep inside the substrate where the water will not evaporate off quickly. Wouldnt this mean that every bulk sub should get contaminated some time after the second flush?
I see sometimes the dark nutrient rich water collect in places in the tub, and it´s reasonable to assume that there exists many places in the sub that can hold in place nutrient rich water. We know that water can not be completely colonized by the myc so that nothing else can set in.
So is this the reason why bulk substrates contaminate after a while?
-------------------- Cakes inside Water Tub
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: Mateja]
#24924165 - 01/18/18 07:58 PM (6 years, 12 days ago) |
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Not the main reason...mycelium networks are very genius at moving water around to where it needs to be
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: grainbrain] 1
#24924193 - 01/18/18 08:09 PM (6 years, 12 days ago) |
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Quote:
grainbrain said:
Quote:
mushboy said:
its not about that. by standard definition i have awful tek. grainbrain just said that. but yet i dont have any of these problems you guys encounter and bitch about so much. im a fuckin tard sometimes but yet.... i dont have these issues.. its not boasting dude. its trying to share a lesson i learned.
a lesson a lot of people on here seem to ignore.
First of all, I didn't say anything about your technique, but since you brought it up... I've seen your grows over the last couple years here and you really should not be taking lead in giving anyone advice.
Furthermore, this isn't the first time I've called you out here for shitting all over someone else's technique and being belligerent in your own ignorance. The most recent case being Morelman's GH grow where you spent plenty of time telling him that his bacterial substrate was not going to fruit & that he should trash it. You were wrong, this happens a lot.
You spend all day, every day here and have a lot of ego invested in your online persona. This can be detrimental to the collective knowledge base, especially when you're shouting down others with your "lessons learned".
Good luck in your future endeavors.
No one cares what you think of some personality you never met or never will. Drop out of the debate or keep it to the topic. Someone saying your technique sucks over the internet shouldn't bother anyone. Either you're confident in your technique and you're hear to teach or you're not and your are hear to learn
No one gives a fuck if you think he is ignorant or egotistical. If he is he will become ignored or you can ignore him and then it's not your problem. Don't be a seed for people to go back and forth with insulting each other
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: cronicr] 1
#24924200 - 01/18/18 08:12 PM (6 years, 12 days ago) |
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Quote:
So is this the reason why bulk substrates contaminate after a while?
I think the main reason is just that the entire network gets weaker after flushing, making it more susceptible to contaminants. That's the very basic explanation.
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pacmanbreed


Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
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Re: Cultivation General Discussion [Re: mushboy]
#24924203 - 01/18/18 08:15 PM (6 years, 12 days ago) |
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Quote:
mushboy said: you can tell i dont spray my box cause my dishes would have that odd glare shit(water drop stains??). that happens when you 'mist' petris

Mush hope you dont mind. Where to you use the clorox wipes? Ive heard good about them. Im planning to buy them to lessen my papertowels.
Edited by pacmanbreed (01/18/18 08:18 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: TheDarkHeart]
#24924209 - 01/18/18 08:18 PM (6 years, 12 days ago) |
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Quote:
TheDarkHeart said: if you plan on answering all my questions then you are fuking awesome. you would be the first to do that. i'm asking so many questions. i'm brand new to this.
Can polyfil touch substrate? -yes but people try to avoid shrooms stuck in poly
Can a liner be cut high if the holes with polyfil are too low for a good spawn to coir ratio when in a FC? -my tubs don't even use holes, or liners. But if you're using a traditional mono just make the liner the right way if you're doing the liner thing.
is 86 degrees Fahrenheit to hot for a grow room with a FC that has a light over it? -yes aim for 68-72 above 75 i put the AC on
what does too much spraying look like. tiny droplets on stuff, or puddles? -youll learn. Puddles. Judge your substrate by weight too.
during FC colonization of sub it should not be aired out and it should get 2 sprays in the morning and 2 at night? -lol no, there's no schedule. During colonization leave it completely alone. For fruiting phase Do things as needed. Chances are you don't need to do a damn thing most days if at all. Airing it out is also pretty pointless it gets fresh air exchange 24/7
I'm using bucket tek for coir. -using 4 sprays a day during colonization is probably the maximum you should spray? -no spray during colonization...
what is the best coir to spawn ratio? 1:1 to 1:4 works. Best doesn't make sense everyone has their own idea of best.
on the first grow i got complacent and didn't realize my neglect and not following common practices to the t was a big deal. whoops. the whole thing dried out and i think it's irrecoverable. -post pics along the way
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: pacmanbreed] 1
#24924210 - 01/18/18 08:18 PM (6 years, 12 days ago) |
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Haha the clorox thing is full of clean gloves. Haha. A remaining artifact from my newbdom.

dont use clorox wipes
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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It seems also reasonable to assume that some of the nutrient rich water will get trapped inside the coir as well and the parts that are not colonized by mycelium will be perfect germination spots for other spores or for live bacteria as well. Idk I´m mostly speculating and reading the responses
-------------------- Cakes inside Water Tub
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Mateja]
#24924234 - 01/18/18 08:34 PM (6 years, 12 days ago) |
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Not if there's enough air exchange. If the water stands/pools/stagnates the potential for said problems occur.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: hamloaf]
#24924256 - 01/18/18 08:41 PM (6 years, 12 days ago) |
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But even with enough FAE, coir that´s been hydrated with nutrient rich water will still remain hydrated, it wont dry out. And thus will serve as a point for germination. Mycelium is lazy about recolonizing every part of the substrate after the first dunk. It leaves spots of uncolonized bulk substrate in a few places.
-------------------- Cakes inside Water Tub
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: Mateja]
#24924265 - 01/18/18 08:45 PM (6 years, 12 days ago) |
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It may look uncolonized but it isn't...also the water would be full of enzymes leached? I do not something I have ever cared to think about lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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pacmanbreed


Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
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Re: Cultivation General Discussion [Re: mushboy]
#24924316 - 01/18/18 09:16 PM (6 years, 12 days ago) |
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Quote:
mushboy said: Haha the clorox thing is full of clean gloves. Haha. A remaining artifact from my newbdom.

dont use clorox wipes
artifact has found its purpose. Thanks for the tip.
Anyways this is posted on q&a Planing to clean my plates while have free time. This are all 2nd transfers from cubensis filty spores. Ive already made 3rd transfer waiting for growth. Still practicing identifying growth patterns. But Seems im facing two kinds of fungus growing from the center.
 - are there any usefull cubensis growth from this?
Im planning to ditch some of the 3rd 4x plates to keep me from wasting dishes and time. Thanks.
Edited by pacmanbreed (01/18/18 09:17 PM)
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: pacmanbreed]
#24924324 - 01/18/18 09:19 PM (6 years, 12 days ago) |
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Those rings are scary something anit right.
Do you have temperature swings?
Oh I forgot to respond earlier.
always enjoy the discussion guys.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Re: Cultivation General Discussion [Re: van hatton] 1
#24924336 - 01/18/18 09:27 PM (6 years, 12 days ago) |
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Will temperature swings cause rings like this?
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pacmanbreed


Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
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Re: Cultivation General Discussion [Re: van hatton]
#24924391 - 01/18/18 09:46 PM (6 years, 12 days ago) |
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Yes shomman temp swings also cause rings.
Quote:
mushboy said: this one has temp rings.

this one also has 'rings' but its from hidden nasties and whatnot.

Quote:
Explicitdetail said: Yea I thought spawn might have been the issue but seemed like it was more related to casing than spawn. All my projects are on hold till after the holidays so maybe I’ll go back to spores instead of the current plates I have. Here is the plate I used for the tub in question

Quote:
van hatton said: Those rings are scary something anit right.
Do you have temperature swings?
Oh I forgot to respond earlier.
always enjoy the discussion guys. 
Thanks for the reply van. Yes they are receiving multiple temp swings. They are kept in the unstable ac bedroom. since Its hot here. 80f ac on. Then 88f ac off multiple times a day. Damn need to invest for some stable cool portable storage for my plates.
Edited by pacmanbreed (01/18/18 09:57 PM)
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