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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Mateja]
#24923917 - 01/18/18 06:12 PM (6 years, 12 days ago) |
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Also wiping with iso is also helpful for physical removal of contamination. Including things that may not be killed.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Mateja]
#24923920 - 01/18/18 06:14 PM (6 years, 12 days ago) |
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Quote:
Mateah said: I saw a documentarie yesterday about how micro chips are made. Anyway the production rooms need to have around 100 particles per cubit foot, thats very little, I believe there are 30.000 per cubic foot in the home. I believe that the particles in our operational SAB are also some thing like a few houndred particles per cubic foot. Or do you think itΒ΄s less bod?
Particles per cubic foot is astronomical. Its not a sterile air box. Technique makes the problem go away.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: bodhisatta] 1
#24923931 - 01/18/18 06:17 PM (6 years, 12 days ago) |
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I will now go and crack a bunch of plates inside my SAB and leave them on the rack with the lid off for. 15sec, 30sec, 60sec, 2min, 5min and 10min and 20min. IΒ΄ll post updates in a week or so.
I already have a bunch of 1% MEA plates that I wasnt gonna use anyway, I almost felt sry for the mycelium when I saw it on the 1% plates compared to the 2% plates. Now the plats donΒ΄t go to waste haha
-------------------- Cakes inside Water Tub
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Mateja]
#24923937 - 01/18/18 06:18 PM (6 years, 12 days ago) |
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I would imagine the contamination to be prevalent in the dishes open for 15+ seconds.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: bodhisatta]
#24923972 - 01/18/18 06:31 PM (6 years, 12 days ago) |
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Mushboy did one for 45 minutes. Stayed clean.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: van hatton]
#24923988 - 01/18/18 06:39 PM (6 years, 12 days ago) |
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you can tell i dont spray my box cause my dishes would have that odd glare shit(water drop stains??). that happens when you 'mist' petris
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Cultivation General Discussion [Re: mushboy]
#24923997 - 01/18/18 06:42 PM (6 years, 12 days ago) |
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Quote:
mushboy said: over thinking is good. overthinking keeps me from introducing a flaming red hot object into a confined space with a flammable vapor.
If you really think about the dynamics here, it's not an issue. I worked that way for three years before the flowhood.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: grainbrain]
#24924004 - 01/18/18 06:44 PM (6 years, 12 days ago) |
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spraying iso into a box and sticking a flamed blade inside is ok?
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: mushboy]
#24924008 - 01/18/18 06:46 PM (6 years, 12 days ago) |
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Quote:
mushboy said:
Quote:
AyePlus said: Wow you must think Iβm a fucking moron. thumbs down to you too, dick.
i didnt say that. i know you think wiping down a petri is solving a problem but it isnt. so his plates were out for two months. so what. so are mine. i dont wipe them. the practice is foolish
having 'contams' or whatever on the outside of the dish doesnt matter if you have proper technique. as madhatter said, his sab results were bad. and hes 'ok' with a hood? you spend all that money on a hood to label yourself with 'fairly good sterile tek'?

in fact? just about nothing matters if your technique is down. so im a dick because i know how to do sterile work inside a SAB?

I call it fairly good because I am able to admit I am not going to be 100% perfect. I am trying to keep my ego in check instead of boasting I have perfect or really good sterile tek. It is humbling ones self or some shit like that. I know I do not move dirty shit over clean sterile agar. I know that with a hood I hardly ever see a contamination without having transferred from an all ready contaminated plate, in pursuit of cleaning the culture. Honestly I guess I should of said I do have really good sterile tek as I do, just didn't want to sound like an ass saying it.
-------------------- JOIN THE POW WOW
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Cultivation General Discussion [Re: mushboy] 2
#24924021 - 01/18/18 06:52 PM (6 years, 12 days ago) |
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Quote:
mushboy said: spraying iso into a box and sticking a flamed blade inside is ok?
Yes. The blade is cool enough by the time it gets inside the box.
Standard procedure was to spray down the SAB, let it settle for 10-15min and then begin work. I don't get why this is so difficult to comprehend.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: grainbrain]
#24924026 - 01/18/18 06:53 PM (6 years, 12 days ago) |
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because that standard practice you laid out is pointless! why is that so hard to comprehend?
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Quote:
TheMadHatter420 said: Honestly I guess I should of said I do have really good sterile tek as I do, just didn't want to sound like an ass saying it.
False modesty is a sin my child
-------------------- Cakes inside Water Tub
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Quote:
TheMadHatter420 said: I am trying to keep my ego in check instead of boasting I have perfect or really good sterile tek.
its not about that. by standard definition i have awful tek. grainbrain just said that. but yet i dont have any of these problems you guys encounter and bitch about so much. im a fuckin tard sometimes but yet.... i dont have these issues.. its not boasting dude. its trying to share a lesson i learned.
a lesson a lot of people on here seem to ignore.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Re: Cultivation General Discussion [Re: mushboy]
#24924035 - 01/18/18 06:58 PM (6 years, 12 days ago) |
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Hmm spraying is pointless? I'll try not spraying next time, I hate those little water stains.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: 00Burnout]
#24924039 - 01/18/18 06:59 PM (6 years, 12 days ago) |
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Quote:
00Burnout said: Hmm spraying is pointless? I'll try not spraying next time, I hate those little water stains.
make sure your technique and procedure is tight. and youll be good bro ..trust the fungus.
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: Mateja]
#24924042 - 01/18/18 07:00 PM (6 years, 12 days ago) |
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How can we say pour lc without a lid at all or LI as tekβs prescribe and then say that 15+ seconds will contam open plates?
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: mushboy]
#24924045 - 01/18/18 07:01 PM (6 years, 12 days ago) |
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How do you attain your still air if you don't give it time to settle? I could never keep still air having to move plates in and out. I would even set there holding my arms still for 60+ sec, then make my transfer. I got mixed results. SADLY best results I had, in the SAB, was working in the cramped ass bathroom closet spraying the shit out of my SAB with alcohol and working semi slow. When I went to working really slow and using soapy water shit went south.
Are you able to explain how you get some of these things to work? I don't know how some get still air and some of us don't. I guess I moved around to much for a SAB. I guess to me it took more work to get good with a SAB than it did a flow hood. I took the short cut by spending $. Fuck it I will say that at least. I couldn't use a SAB correctly but I damn sure can use a hood correctly. Hell mines not even laminar flow. It is just a 12 inch Nuclear Grade HEPA that's 99.99% down to .3 micron.
-------------------- JOIN THE POW WOW
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: JHOVA]
#24924046 - 01/18/18 07:01 PM (6 years, 12 days ago) |
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or how can i pour LCs in my sab all shaky and shit when i dont spray? nnigga plz
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: mushboy]
#24924047 - 01/18/18 07:02 PM (6 years, 12 days ago) |
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I just don't understand how the fuck to get still air. No matter how much I tried and how slow I worked the results varied.
-------------------- JOIN THE POW WOW
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: grainbrain]
#24924048 - 01/18/18 07:03 PM (6 years, 12 days ago) |
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Quote:
grainbrain said:
Quote:
mushboy said: spraying iso into a box and sticking a flamed blade inside is ok?
Yes. The blade is cool enough by the time it gets inside the box.
Standard procedure was to spray down the SAB, let it settle for 10-15min and then begin work. I don't get why this is so difficult to comprehend.
Spraying your sab with iso is idiotic. The potential bomb is not worth it especially with a flame sterilized xacto.
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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