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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: AyePlus]
    #24923526 - 01/18/18 03:36 PM (6 years, 12 days ago)

Quote:

AyePlus said:
Wow you must think I’m a fucking moron. thumbs down to you too, dick.





i didnt say that. i know you think wiping down a petri is solving a problem but it isnt.
so his plates were out for two months. so what. so are mine. i dont wipe them. the practice is foolish:shrug:

having 'contams' or whatever on the outside of the dish doesnt matter if you have proper technique. as madhatter said,
his sab results were bad. and hes 'ok' with a hood? you spend all that money on a hood to label yourself with 'fairly good sterile tek'?

:confused:

in fact? just about nothing matters if your technique is down. so im a dick because i know how to do sterile work inside a SAB?

:archiebunker:


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Offlinepacmanbreed
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Re: Cultivation General Discussion [Re: van hatton]
    #24923576 - 01/18/18 03:57 PM (6 years, 12 days ago)

Quote:

van hatton said:
Quote:

van hatton said:


Hmm... Wonder if they'll tam out





Update..

Both have small spots of bacteria all over. The camera doesn't pick them up but there's a good 10-15 spots per plate. :shrug:




You work is smooth. Except for the few  sliding,
Its a possibility that bacterial spores came from the lower rims of the lid?

Its magical. Make me also wanna play on my blanks to test my movements.


Edited by pacmanbreed (01/18/18 04:49 PM)


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: mushboy] * 1
    #24923581 - 01/18/18 03:59 PM (6 years, 12 days ago)

Musgboy, No matter how comfortable you are with your technique you can't deny that iso kills bacteria


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24923591 - 01/18/18 04:03 PM (6 years, 12 days ago)

Quote:

tryptkaloids said:
you can't deny that iso kills bacteria





i didnt say that either.  wtf.

why do people put words in my mouth(text) just to prove a minimal point that i never brought up???
iso or not, shit on the outside of a dish isnt causing a problem. the technique is, the grower is. you are.


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: mushboy] * 1
    #24923605 - 01/18/18 04:07 PM (6 years, 12 days ago)

The grower is outside the dish... It's coming from outside. Taking steps to aid that can help. That's all I'm saying.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleMateja
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24923623 - 01/18/18 04:15 PM (6 years, 12 days ago)

He had a bunch of bacteria even on the receiving plate that had minimal exposure, his technique on the receiving plate was good enough and should have produced a clean plate if the tools above the media were sterile and if the air inside the SAB was still enough. It has to be either one of these factors :shrug:


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InvisibleJosex
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Re: Cultivation General Discussion [Re: mushboy] * 1
    #24923633 - 01/18/18 04:16 PM (6 years, 12 days ago)

I don't think we're gomna kill a lot of stuff if we wipe the plates down with iso, certainly not mold spores.
I do wipe my plates though, but only because I use no pours (so there's no cling wrap to protect them) and I leave the plates in open air while they colonize. Wiping them down will remove some nasties from the more problematic  areas (like under the lid) or at least make them nasties stick to the wet surface.
The lids of no-pours don't open as smoothly as the lids of petris, so chances are some shit under the lid may land on the agar if you don't wipe them.


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24923634 - 01/18/18 04:17 PM (6 years, 12 days ago)

i know that dude. my point is that 'aid' is a superstition once you get your technique down. you guys have seen my SAB pouring video yet not a damn one contaminated. i made michael j fox look like a statue i shake so bad. but i dont wipe down petris. at all.

dont spray my box.
dont close vents.
dont do any of that shit.

thats all im saying. maybe all these steps are not solutions. but just pointless steps. thats what my experience tells me and im sharing that.


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InvisibleAyePlusS
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24923637 - 01/18/18 04:19 PM (6 years, 12 days ago)

I think everyone should start with an SAB first because they are so much cheaper and if you can make something that is 8-10$ work instead of spending hundreds then more power to you, it is also a great introduction to sterile work, and a cheap way to wet your feet in the agar game.

I considered buying/building a bigger SAB but I said fuckit and made the next step up because my dayjob pays well enough and now that I’m not boozing and partying every weekend I can afford to splurge and buy fun toys/tools every once in a while, cost me less than a new ipad tv or video game system would and IMO infinitely more valuable. Takes up a little more space and weighs more, but I already had a desk dedicated to mycology.

Everyones situation is different and whatever works best for you is what you should use, a flow hood is no replacement for good technique but it can be a valuable tool in the right situations.

I’ll finish with this quote from Abraham Maslow:
Quote:

  "If all you have is a hammer, everything looks like a nail"




Edit:

@ mushboy if the edge of the plate is dirty couldnt something fall from it onto the plate since it is passing over the agar when you open it? I wasnt sure where his petris had been and He is clearly getting contams from somewhere I’m just trying to troubleshoot. I called you a dick because you made the assumption that I would suggest doing something as silly as opening sterile petris in open air only to wipe them down with non sterile alcohol. Thats just some noob shit, who would do that? Only a moron so it seems you were calling me a noob/moron. Kinda dickish imo. Not tryna start any petty squabbles here just trying to help and don’t appreciate your condescending tone. It works for you we get it.m, bod works without gloves, and azur does his SAB work in the nude. Doesn’t change that I’m gonna wear clothes and gloves and probably take a shower before my agar work, works for thembut doesnt mean that it will work in every situation. See my quote above.

I built/got the hood because of volume, ease of use and comfort. I have 20+ different species am constantly adding more and want to make alot more spawn than I can in a SAB comfortably. I didnt spend much considering the same sized hood I built for ~350 goes for over 1400 on the FP website. Most people probably spend 4-5 times that on fast food in a year. I used to spend more than that on booze and coke every month now I have different priorities...


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: AyePlus]
    #24923644 - 01/18/18 04:22 PM (6 years, 12 days ago)

Quote:

opening sterile petris in open air only to wipe them down with non sterile alcohol




i didnt say that at all. nor did i accuse anyone of that. i knew you were referring to a used, already inoculated dish.

flow hoods do slove problems that was never my point. again, why are people putting words in my mouth.
if anything i said fhs wont solve bad technique. which they wont.


Edited by mushboy (01/18/18 04:23 PM)


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OfflineLeftfield420
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Re: Cultivation General Discussion [Re: mushboy]
    #24923710 - 01/18/18 04:52 PM (6 years, 12 days ago)

I had a similar problem...found out I was spraying nasties all over my sab when spraying soapy water...inside of my spray nozzle had a clear film built up..cleaned it out with bleach...started washing my bottle out with hot water and making sure to spray hot water through it before each sab session


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Leftfield420]
    #24923785 - 01/18/18 05:26 PM (6 years, 12 days ago)

Or, just don't spray :shrug:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineLeftfield420
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24923796 - 01/18/18 05:29 PM (6 years, 12 days ago)

Yeah or that...hell I haven't broke out the sab in a couple weeks...


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Invisiblegrainbrain
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24923798 - 01/18/18 05:30 PM (6 years, 12 days ago)

I used to spray my SAB with ISO & flame sterilize just outside the box.  I think people overcomplicate and overthink these things.  Be the spores, be the air.


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: grainbrain] * 1
    #24923806 - 01/18/18 05:32 PM (6 years, 12 days ago)

over thinking is good. overthinking keeps me from introducing a flaming red hot object into a confined space with a flammable vapor.


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InvisibleJHOVA
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Re: Cultivation General Discussion [Re: grainbrain] * 1
    #24923814 - 01/18/18 05:35 PM (6 years, 12 days ago)

Quote:

grainbrain said:
I used to spray my SAB with ISO & flame sterilize just outside the box.  I think people overcomplicate and overthink these things.  Be the spores, be the air.



Bomb.com


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Offlinepacmanbreed
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Re: Cultivation General Discussion [Re: Leftfield420]
    #24923892 - 01/18/18 06:02 PM (6 years, 12 days ago)

Quote:

Leftfield420 said:
I had a similar problem...found out I was spraying nasties all over my sab when spraying soapy water...inside of my spray nozzle had a clear film built up..cleaned it out with bleach...started washing my bottle out with hot water and making sure to spray hot water through it before each sab session




Good observation. I had similar problems too from stagnant spray bottles.
I only now wipe with soapy water using fresh hanky for short sab sessions. But will defenitely use clean spray bottle for long sessions.


Edited by pacmanbreed (01/18/18 06:04 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Crispykoot] * 1
    #24923895 - 01/18/18 06:04 PM (6 years, 12 days ago)

Quote:

Crispykoot said:
Quote:

tryptkaloids said:
My opinion is that hoods should be used as a means of ramping/speeding up production, not as a means to avoid contams




I guess that's why they are in just about every lab? They are a great tool for avoiding contams and make life easier.

Still air boxes have their place too.



Most labs still use the Bunsen burner sterile field and not even a sab or FH.

Our myco tissue culture work is among some of the hardest sterile work to accomplish. And the requirements are stringent for succeeding.


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: bodhisatta]
    #24923906 - 01/18/18 06:08 PM (6 years, 12 days ago)

Quote:

bodhisatta said:
Most labs still use the Bunsen burner sterile field and not even a sab or FH.





:wtfsonic:


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InvisibleMateja
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Re: Cultivation General Discussion [Re: bodhisatta]
    #24923909 - 01/18/18 06:09 PM (6 years, 12 days ago)

I saw a documentarie yesterday about how micro chips are made. Anyway the production rooms need to have around 100 particles per cubit foot, thats very little, I believe there are 30.000 per cubic foot in the home. I believe that the particles in our operational SAB are also some thing like a few houndred particles per cubic foot. Or do you think itΒ΄s less bod?


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