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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Yup yup
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: van hatton]
#24917682 - 01/16/18 07:58 AM (6 years, 14 days ago) |
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your sab sessions lack heavy metal
do you do a 'ccoling' dip with your flamed blade?
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: mushboy]
#24917705 - 01/16/18 08:13 AM (6 years, 14 days ago) |
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When mycelium is involved yes. Needs more 
This time I didn't give a shit. I'm doing a plate then posting a picture. Then the day that plate tams I'll do another one. Hopefully can really see what the difference is.
The video looks fine to me except I move the right plate over to the paper towel I have chillin.
Just seeing if I'm missing something one of y'all would point it out
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: van hatton]
#24917751 - 01/16/18 08:45 AM (6 years, 14 days ago) |
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That looks good to me. I can't think of a suggestion. I honestly didnt even cool my blade till I got a spear scalpel, they stay too hot for myc to handle. When I do cool I do it on the plate with myc on it. I dont,know why people to it,on the new plate
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24917755 - 01/16/18 08:49 AM (6 years, 14 days ago) |
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i stopped cooling my blade ages ago. flamed till hot, moved into the sab.. open culture... cut culture. if you work juuuuust slow enough the blade will be cool enough to cut into the myc without fuckin with it.
something about opening the receiving dish before 'game time' just always seemed wacky to me.
like, why open the blank media 2 times. thats twice the room for error i guess with a hood it doesnt matter.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: van hatton]
#24917756 - 01/16/18 08:50 AM (6 years, 14 days ago) |
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I 2nd the metal.
You seem to, nervous or unsure of your movements. Maybe clean out some old petri, and pour some agar in them just to practice in open air. The idea being getting yourself more comfortable with your movements and actions. It has taken me a while to feel comfortable and I do like 40-60 transfers a week. The more you practice the more comfortable you will be and in turn should make less drafts via sporadic movement, as well mistakes in general.
Trypt I think that is because they do not want to risk cooling it in anything that could contaminate the blade. I could see your way after the 1st few transfers, but the early ones could have bacteria where you cool?
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RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24917761 - 01/16/18 08:53 AM (6 years, 14 days ago) |
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I never cool my blade either tryp. the first slice into the colonized plate takes care of it. ive never not had growth from doing it this way. I must have bought the shittiest blades ever though. when I stick it in red hot all of this carbon soot shit comes blowing off the blade. still doesn't hurt its just kind of ugly for a while.
edit. my thought exactly mushboy! double the pleasure, double the fun of...contaminants.
Edited by RomeoPapa (01/16/18 08:57 AM)
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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I know everyone says antibiotics don't have a place in this hobby but I disagree for reasons like that.
I use a mix of kanamycin and ampicillin in my plates and I recently put 5 different prints (from god knows who and how clean they were done) onto agar and had all 5 with clean germination plates, free of both bacteria and other fungi.
I use the same plates for transfers and growth to help the possibility of taming out (from bacteria).
Works well for me and due to my lab accessibility, it is easy to add and use. I think someone with an SAB would benefit from antibiotic plates, maybe it is overkill for those of us using a flowhood.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: RomeoPapa]
#24917776 - 01/16/18 08:59 AM (6 years, 14 days ago) |
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I get that to. After a bit I will heat and cool a couple times in the alcohol and it helps get it off. You may also notice we are changing the molecular structure of the blades by heating them glowing red. They will snap when new but once glowing red and cooled they will bend. The more you do it, the easier it is to bend. I think we are actually removing zinc or carbon, not really sure, from the surgical steel when we heat and cool it. I am not really sure what goes into surgical grade steel, and there are varying steel recipes. I just know we are altering the metal and that's hit is something we are removing.
I used antibiotics for a bit but have gotten my skills to where I do not need them. IF I ever use them again, it would only be germination plates. I have noticed wild prints tend to only give me mold, some bacteria. While indoor produced prints give me more bacteria than mold.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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I haven't done a regular transfer in like 2-3 months so that could be part of it. I also need to check my G2G because bacteria.
If these grains I pced for 3 hours go bacterial I can assume it's my procedure.
Edit: I should do blank lc pours (pouring blank lc into blank lc)
They are unforgiving so I'd know quickly if I fucked up.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (01/16/18 09:24 AM)
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Mateja]
#24917839 - 01/16/18 09:26 AM (6 years, 14 days ago) |
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Quote:
Mateah said: Ahh it´s those lids that snap down and lock in place after a really short turn, and open as easy. And clamp down like a madrfakr, more hermetically than any other lids I´ve seen. I bought 24 of them but for 3dl jars, just gotta find me some qt jars that match the lids 
Josex are your lids also coated on the inside with some rubbery plastic all over?
Sactly dude, that's the description right there, could not have put it better myself. Love the 'short turn'. They're also durable as hell.
How is it you found lids separately? It's always jar and lid for me. Whatever, you need to get a fleet of jars for those lids cos they kick fucking ass.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: van hatton]
#24917949 - 01/16/18 10:25 AM (6 years, 14 days ago) |
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Quote:
van hatton said:
Hmm... Wonder if they'll tam out
I would have something on the rack so they don't slide around, keep the receiving jar a little farther away, and make an actual triangle cut into the agar as it looked like you just scraped after a single slice. Looks good, though.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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It does look like I only slice once 
It is a triangle i slice 2 times and on the third slice I scoop as well.
I was thinking about putting some silicone on the rack itself too try to keep them in place.
As good as it looks with my history their will be mold on the plates. But we'll see.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: van hatton]
#24917974 - 01/16/18 10:37 AM (6 years, 14 days ago) |
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I simple paper towel soaked in alcohold will prevent the plates from sliding around.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Josex]
#24917979 - 01/16/18 10:40 AM (6 years, 14 days ago) |
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Anyway, you guys still using SAB's and hoods? That's outdated mang.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Josex]
#24917992 - 01/16/18 10:46 AM (6 years, 14 days ago) |
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-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: van hatton]
#24918004 - 01/16/18 10:51 AM (6 years, 14 days ago) |
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my next thought process for sterile work is working in zero g.. maybe the X in josex is like the X is spaceX.
are you an ancient astronaut?
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Germs
Space Force



Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: mushboy]
#24918070 - 01/16/18 11:20 AM (6 years, 14 days ago) |
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“SAB’s and hoods are crutches used by noobs stuck in the 2010’s of growing. Use my easyAF zero gravity aerobatics open air inoculation TEK. It’s easy as fuck.”
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Germs]
#24918085 - 01/16/18 11:26 AM (6 years, 14 days ago) |
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Im just working on the protection from cosmic ionizing radiation
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: bodhisatta]
#24918105 - 01/16/18 11:37 AM (6 years, 14 days ago) |
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You could build a sterilzer that the jars inside are all connected via hose to a manifold then to a hose that goes external and can be closed via a valve during sterilization.
This would accomplish numerous advantages theoretically.
When coming to pressure you could pull a small vacuum on the line this would help remove air from inside the spawn jars or bags aiding the venting step. Close the valve and bring to pressure.
Once the cycle is done you could put positive pressure on the line of sterile filtered air. This would speed cool down and your jars would never experience vacuum.
Once cooled to room temperature you could switch from keeping pos pressure on the cooling jars to inoculation. A central LC bioreactor would be fed to all the jars.
Then the jars could be taken out of the steriliser already inoculated as aseptically as possible.
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