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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: AyePlus]
#24913496 - 01/14/18 12:55 PM (6 years, 16 days ago) |
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Spore solution to grain is ms. Spore solution cleaned up on agar and transferred to grain is limited ms.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 11 hours
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Re: Cultivation General Discussion [Re: natedawgnow]
#24913499 - 01/14/18 12:57 PM (6 years, 16 days ago) |
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The we should see that more
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24913503 - 01/14/18 12:59 PM (6 years, 16 days ago) |
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limited ms.. lms. ... ack.
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: mushboy]
#24913513 - 01/14/18 01:05 PM (6 years, 16 days ago) |
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Quote:
mushboy said: limited ms.. lms. ... ack.
If it aint broke don’t fix it
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Mateja]
#24913515 - 01/14/18 01:06 PM (6 years, 16 days ago) |
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Quote:
Mateah said: Can we start saying “inoculated with spore solution” instead of MS to grains? I’m still confused when people mean ms culture on grains and when they mean spore solution twas grains  Or have I missed something?
That's fine.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 3 hours
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Re: Cultivation General Discussion [Re: Josex]
#24913548 - 01/14/18 01:27 PM (6 years, 16 days ago) |
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Quote:
Josex said: Foil won't do anything to keep mold spores from reaching the lid, that's absurd. It's serves as a dust cover, that's about it.
actually the foil is very counter productive as it can easily cause your filters to get wet from the moisture in the jar and then the filter will fail when wet.
On crispys case I have had some issues with failed filters(sfd) in a room with a air purifier fan blowing into the room. Unopened and uninoculated they would mold on the top of the grains.
I think it may have been actually going through the threads as the problem slowed majorly after wrapping in saran wrap. but I still ended up with some failed test jars so I thought it must also be a filter issue. So I then tested just sfds against stiff felt and then tested both of those again with a couple layers of micropore.
Well I found that the sfd's and stiff felt were failing some percent of the time and the micropore fixed that.And the reason those were failing was probobly the fan and possibly worn out sfds.
I have had my jars with sfd/stiff felt and two layers of micropore last for 6 months and no signs of contam. still have the fan (or a massive flowhood) running most of the time and there is not a single failed test jar since adding micropore.
I still haven't tried any without saran since adding the addition of micropore so I'm not totally sure if they were in fact also failing through the seal in threads in the lid.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 3 hours
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Re: Cultivation General Discussion [Re: Mateja]
#24913574 - 01/14/18 01:38 PM (6 years, 16 days ago) |
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Quote:
Mateah said: Can we start saying “inoculated with spore solution” instead of MS to grains? I’m still confused when people mean ms culture on grains and when they mean spore solution twas grains  Or have I missed something?
I totally agree with this.
I really don't understand how that is the term for spores to grain.
MS is just that multispore. Multigenetics and no isolating.
Of course there is slight isolation/narrowing going on when cleaning up a culture on agar but not enough to call it any less than ms.
MS to grains should not entail dirty spores or a syringe. IT is just multispore in any form to grains. not specifically a dirty syringe.
If referring to a ms syringe to grain maybe we should call it something more along those lines.
like "ms syringe to grain" is it really that hard to type syringe?
or I like what mateah said: Spore solution!
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Edited by tombosley8 (01/14/18 01:46 PM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tombosley8]
#24913575 - 01/14/18 01:38 PM (6 years, 16 days ago) |
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a single post office large tyvek envelope can make you hundreds of little squares to cover your jar holes. use tape like bosley said and thats a near full proof filter that you can run over and over yet, was free in the states
i change the tiny sqaure and tape every 4 or 5 runs. takes like 3 seconds. MP tape gets expensive i guess but 1 roll last me well over a year.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 3 hours
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Re: Cultivation General Discussion [Re: mushboy]
#24913586 - 01/14/18 01:43 PM (6 years, 16 days ago) |
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Except I use stiff felt(or sfds) in place of your tyvek and it works great.
I melted my filter to the inside of the lid so on the topside I only have a small hole to cover with micropore.
I just lay out a strip on a peice of wood, double it up and then use a razor blade to cut out little squares.
is so easy and the micropore really stands up to multiple cycles and wet conditions very well.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: mushboy]
#24913589 - 01/14/18 01:43 PM (6 years, 16 days ago) |
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SFDs are once and done. They last a lifetime.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tombosley8]
#24913590 - 01/14/18 01:44 PM (6 years, 16 days ago) |
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word. id use sfds/felt if i couldnt get free tyvek literally a block away. thanks usps!
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: hamloaf]
#24913592 - 01/14/18 01:45 PM (6 years, 16 days ago) |
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Quote:
hamloaf said: SFDs are once and done. They last a lifetime.
isnt that kinda naive to think? thats like saying a light bulb should last forever. how do sfd companies make money if they last a lifetime?
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 3 hours
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Re: Cultivation General Discussion [Re: hamloaf]
#24913598 - 01/14/18 01:48 PM (6 years, 16 days ago) |
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Quote:
hamloaf said: SFDs are once and done. They last a lifetime.
I have had many failures with sfd's as I explained and the solution was adding a couple layers of micropore. Maybe it is the fan that is causing the issue then.
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The Sloth
Stranger



Registered: 12/29/16
Posts: 12
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Re: Cultivation General Discussion [Re: Crispykoot]
#24913609 - 01/14/18 01:52 PM (6 years, 16 days ago) |
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Not to really jump in on the airflow/environment/spore load vs. filter debate, but what I've done with my agar and grain jars is store them in shoeboxes and totes to minimize the airflow as a factor. Everyone probably has extra on hand anyway. I haven't ever had an issue with contams that I suspected from the environment (just sloppy technique), but this just seemed to make sense to keep cat hair/bacteria/etc off the outside of the jar in a dirty apartment. It has given me a bit of extra peace of mind when doing the wipe down before doing inoculations and transfers. Just a thought.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: tombosley8]
#24913614 - 01/14/18 01:53 PM (6 years, 16 days ago) |
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SFDs and all American pressure cookers will out last you.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: The Sloth]
#24913618 - 01/14/18 01:54 PM (6 years, 16 days ago) |
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You guys should quit being cat people, that's all.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 3 hours
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Re: Cultivation General Discussion [Re: Josex]
#24913624 - 01/14/18 01:55 PM (6 years, 16 days ago) |
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?
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: hamloaf] 1
#24913631 - 01/14/18 01:58 PM (6 years, 16 days ago) |
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Quote:
hamloaf said: SFDs and all American pressure cookers will out last you.
I've been using whole SFDs for 2 years now. I don't bleach them or clean them in any way and they're taking a lot of rust from the metal lids of my mason jars. Still they work as well as the first day.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: tombosley8]
#24913633 - 01/14/18 01:59 PM (6 years, 16 days ago) |
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Quote:
tombosley8 said: ?
Quote:
...this just seemed to make sense to keep cat hair...
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: mushboy]
#24913643 - 01/14/18 02:02 PM (6 years, 16 days ago) |
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Quote:
mushboy said:
Quote:
hamloaf said: SFDs are once and done. They last a lifetime.
isnt that kinda naive to think? thats like saying a light bulb should last forever. how do sfd companies make money if they last a lifetime?
Think it's naive to say something is naive if no experiance is had with something. That's also by definition ignorant. Comparing SFDs to light bulbs is like comparing apples to oranges. New people entering this hobby is one way how they keep selling. Also, people tend to make mistakes with things and are quick to blame the tool rather than their own procedures.
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