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TheBlackCat
Possibly Human



Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: 00Burnout]
#24814291 - 11/29/17 11:39 AM (6 years, 2 months ago) |
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To spawn or not to spawn. I'm making sawdust bags for my oysters and lions mane finally. The damn oysters have been in the jar since the 11th. Hurt my back and they went a bit crazy and are really consolidated. To spawn or not to spawn. Looks like a little pink bacteria but no mold. This is a good strong commerial strain. It should be fine right.
Also I want to make jars from a really nice cube plate I have that grew off of a cloned mushroom that grew on a plate. Should I transfer it again or just use the plate. It's so nice and a mono culture as really clean.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24814477 - 11/29/17 12:50 PM (6 years, 2 months ago) |
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I doubt it's a mono-culture. Your wording is confusing though. I would make a few transfers and keep a few plates as masters. I would spawn the consolidated oysters as well.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Cubensis on lignicolous materials + trace sugar test, some drying out where the paper is disconnected. However very uniform radial from multiple spores, cyan in speed, but visibly normal cubensis.
Needs further investigation, however I got the genetics I was looking for.
Open air assembly, with trace sugar, no mold-other infection.
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TheBlackCat
Possibly Human



Registered: 10/05/16
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Well it's fairly close. It all grew together. I've been transferring this KSSS for awhile now. This is like the 4th or 5th transfer already and I have some other jars that look as nice from the same jar of pins. Yes. I can see how that's confusing. I have a jar 3rd or 4th transfer maybe that grew cute little pins which I snatched up and made 4 jars from. They all look excellent. I need clean plates now to make jars.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24814599 - 11/29/17 01:58 PM (6 years, 2 months ago) |
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My current notes, as I see it.
Cubensis does not appear to expel laccase, however it does oxidize laccase requiring materials, during growth.
Alternative non-laccase requiring carbon sources are starch and sugar, glucose. Cellulose is mostly inert, non-reactive, but also composed of glucose.
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Normally cellulose is met with lignin, and other phenol's, these phenol's require laccase (wood loving enzyme). The same materials are usually acidic, or tolerant, and can be inhibitory depending on the plant.
Therefor under normal circumstances cubensis would have no chance to decay the cellulose. The acidic materials also attract germination of wood loving (laccase) spores.
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Gourmet growers don't even attempt to grow from 100% grain or grain + verm setups. They don't yield the same, fruits don't taste as rich, generally it's not ideal.
The most potent mushrooms also grow from cellulose rich materials.
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Some notes:
We know wood is out of the question without modification, and time-money spent, but both coir and paper are in. There is also the fact that starch becomes less effective over time, due to pH changes in the substrate.
It's also a good idea to germinate on the end media to produce desired genetics. Germinating on starch or sugar may produce media specific growth.
You practically guarantee end media "fresh" genetics.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Ferather]
#24814641 - 11/29/17 02:21 PM (6 years, 2 months ago) |
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Paper vs coir, not much really, however coir is slightly acidic, the paper more neutral, according to TC's "pet bedding" is ideal. Coir is slightly better than paper for enzymes, if the end pH is 6.0-6.5, which is ideal for hybrid cellulase enzymes.
The pH is high enough to reduce germination of laccase expelling spores in general, across the board. There is a small allowance for trace, 1%, sugar-starch, if exposed, or 10% if being aseptic.
The cellulose (coir in this case) should be enriched, depending on your methods.
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Leeching may be more ideal, alternatively add CaCO3, or do both.
Edited by Ferather (11/29/17 03:28 PM)
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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Re: Cultivation General Discussion [Re: Ferather] 1
#24814654 - 11/29/17 02:30 PM (6 years, 2 months ago) |
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If you find some universal primers for laccase I’ll order them and test to see if the P. cubensis genome harbors the enzyme.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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T-Gel, Recipe A, with 2-4g 240B gelatin not 2g agar, no added sugar. Not ideal for anything but laccase oxidization testing. However I can make quick transfers using wood pegs, it's not ideal as gelatin "can" germinate things.
Here are some images of Tarragon oyster on T-Gel (gelatin based) agar, with oxidization. A sweet sugar smell was being produced with the oxidization, due to conversion.
Refer to my T-Gel antibacterial agar link, and swap to 240 bloom gelatin. You will need to keep it at about 20°C, it will be slow to grow.

It will eventually turn to a liquid over time.
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You can also just do normal agar, Recipe A, no ring, but you do get extremely slow growth.
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Pleurotus euosmus (Tarragon oyster) is a wooding loving, laccase expelling mycelium. Here is a side-by-side:
Cubensis var Burma, tea bag, spent in 250g boiling hot pH 8.4 water. Then added to 100g boiling hot pH 8.4 water water + 4g sucrose.

Pleurotus euosmus, tea bag, spent in 400g boiling hot pH 8.4 water. Then added to 100g boiling hot pH 8.4 water water + 4g sucrose.

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250g soak wins.
Edited by Ferather (11/29/17 03:35 PM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Ferather]
#24814773 - 11/29/17 03:47 PM (6 years, 2 months ago) |
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I wonder if some mycelium are built to "tolerate" inhibitory phenol's, rather than directly use them. Then at a later stage, consume them alternatively, and appear to grow on, say wood.
Funny part is the grounds is never 100% wood or the same wood, it varies.
As far as I can tell cyan's don't act well on some materials. They also appear to fail at penetrating solid wood.
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I think they like decayed, leeched, weathered wood, plus leaves and other nutrient rich sources.
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Edited by Ferather (11/29/17 04:05 PM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24814846 - 11/29/17 04:28 PM (6 years, 2 months ago) |
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Quote:
TheBlackCat said: Well it's fairly close. It all grew together. I've been transferring this KSSS for awhile now. This is like the 4th or 5th transfer already and I have some other jars that look as nice from the same jar of pins. Yes. I can see how that's confusing. I have a jar 3rd or 4th transfer maybe that grew cute little pins which I snatched up and made 4 jars from. They all look excellent. I need clean plates now to make jars.
Oh, sorry, didn't see you'd already been transferring. So wait, it's 4th or 5th transfer from a clone? And you've put those plates to grains, which grew in-vitro pins, and you cloned those as well? Sorry, still having a hard time following what is going on.
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TheBlackCat
Possibly Human



Registered: 10/05/16
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Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: stareatclouds] 1
#24814859 - 11/29/17 04:32 PM (6 years, 2 months ago) |
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Hahaha. The agar jar grew pins. There was nothing involved in this scenario but agar. These jars are growing from pins taken from agar. So all sterile.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24814868 - 11/29/17 04:39 PM (6 years, 2 months ago) |
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What agar was that? Also was there any potency, or bruising if not eaten?
Edited by Ferather (11/29/17 04:51 PM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: TheBlackCat] 1
#24814902 - 11/29/17 04:47 PM (6 years, 2 months ago) |
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Quote:
TheBlackCat said: Hahaha. The agar jar grew pins. There was nothing involved in this scenario but agar. These jars are growing from pins taken from agar. So all sterile.
Oh, okay. The word "jars" referring to agar growth is confusing since jars are.. jars. Even if your agar is in jars, might want to call them plates to avoid confusion.
Ok, so an MS plate grew pins and you took transfers. Yeah, could be a monoculture, but tough to say without pictures.
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TheBlackCat
Possibly Human



Registered: 10/05/16
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Last seen: 1 year, 5 months
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Pretty sure it is. Completely solid.
As for what agar I use MYPA. I accidentally bought bacteriological animal peptone. Mycelium seems to like it so fuck it. I use malt syrup.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24814925 - 11/29/17 04:57 PM (6 years, 2 months ago) |
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Thanks, is this the recipe? It's quite and interesting one.
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TheBlackCat
Possibly Human



Registered: 10/05/16
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Re: Cultivation General Discussion [Re: Ferather]
#24814958 - 11/29/17 05:16 PM (6 years, 2 months ago) |
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Edited by TheBlackCat (11/29/17 05:17 PM)
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mushroom_therapy
Apprentice


Registered: 03/23/17
Posts: 1,526
Last seen: 9 months, 24 days
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24815744 - 11/30/17 12:33 AM (6 years, 2 months ago) |
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Any1 ever try extracting psilocin/psilocybin from colonized grain jars using ever clear?
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LemurLemur
Pray for Boog



Registered: 01/30/17 
Posts: 6,004
Loc: Drinking on the roof
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Why? The amount in it is negligible compared to fruits. Thats walking backwards
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
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Re: Cultivation General Discussion [Re: LemurLemur]
#24815867 - 11/30/17 03:51 AM (6 years, 1 month ago) |
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MT could grow stones...Stealthy...
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Shadowboxing the apocalypse and wandering the land
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,584
Loc: Canada
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Quote:
mushroom_therapy said: Any1 ever try extracting psilocin/psilocybin from colonized grain jars using ever clear?
There was a crazy Tek with a culture vat & they extracted Psilo from mycelium. Complex and expensive startup compared to fruits
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