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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: cronicr]
    #24810173 - 11/27/17 05:18 PM (6 years, 2 months ago)

And temperature swings. And probably lighting rhythm. Etc..

Most of us ive seen say something along the lines of "try lowering nutrition if you want to see more rhizo" hardly a fact let alone a rule


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: bodhisatta]
    #24810184 - 11/27/17 05:24 PM (6 years, 2 months ago)

Yeah really only a quick fix or tool for agar work


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OfflineFerather
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Re: Cultivation General Discussion [Re: cronicr]
    #24810205 - 11/27/17 05:32 PM (6 years, 2 months ago)

I agree with cron, there is more to it than "just" nutrients. For example "evasive" rhizomorphs do exist.
It's an extra thick strand or root like mycelium, it's thicker to take more damage (tank).

Not often seen, however I have seen others with infected images.


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Offline00Burnout
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Re: Cultivation General Discussion [Re: bodhisatta]
    #24810217 - 11/27/17 05:39 PM (6 years, 2 months ago)

Quote:

cronicr said:
Many other things to consider besides nutrtion like ease of access ..i believe it was agar who thought about if all things were equal nute wise that density played its part say like when it hits a hard clump of manure it decides to go rhizo so basically doing this switch as needed...we also see changes with co2 levels



Kinda coincides with my mashed potato analogy. How much more work does the mycelium do to use nutrients stored in grain as opposed to agar? I feel like with grain the "stretching out" would still be a feasible argument, do you pick for every bit of meat on a chicken leg or do you eat the easiest bits and go for the next?

Quote:

bodhisatta said:
And temperature swings. And probably lighting rhythm. Etc..

Most of us ive seen say something along the lines of "try lowering nutrition if you want to see more rhizo" hardly a fact let alone a rule



I think I've seen you change your profile pic more this month than I have since I joined the shroomery. Got tired of people getting butt hurt over the "jesus gives himself a handjob" pic or are you seeing how many people you can offend/get ignored by before the new year? Lol


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Offline00Burnout
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Re: Cultivation General Discussion [Re: Ferather] * 1
    #24810223 - 11/27/17 05:41 PM (6 years, 2 months ago)

Quote:

Ferather said:
I agree with cron, there is more to it than "just" nutrients. For example "evasive" rhizomorphs do exist.
It's an extra thick strand or root like mycelium, it's thicker to take more damage (tank).

Not often seen, however I have seen others with infected images.




I've noticed more grain jars here lately that have that type of growth. Looks cool, but if I never see it in person I'll be a happy man. Contams piss me off, I hate wasting things.


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OfflineFishLevelMidnight
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24810238 - 11/27/17 05:47 PM (6 years, 2 months ago)

I’m just speculating here and kind of piggybacking off someone from earlier,

But rhizo growth on grain could be a combination of the myc “stretching” to colonize the substrate quicker; in nature the myc would be competing with a plethora of other species, so colonizing any available food source ASAP would be evolutionary beneficial.

Also most of the nutrition in the grain is stored within it. Maybe the myc goes rhizo searching for food while the colonized grains are broken down?

How exactly does grain get digested by Cubensis myc anyways? Does it just release enzymes on the outside and slowly eat away at it? Or does it penetrate into the grain to release enzymes?

Anyone ever bisected a fully colonized piece of grain?


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InvisibleMateja
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Re: Cultivation General Discussion [Re: cronicr]
    #24810245 - 11/27/17 05:52 PM (6 years, 2 months ago)

Quote:

cronicr said:
Many other things to consider besides nutrtion like ease of access ..i believe it was agar who thought about if all things were equal nute wise that density played its part say like when it hits a hard clump of manure it decides to go rhizo so basically doing this switch as needed...we also see changes with co2 levels




Thanks cron! This makes sense and answers a lot of questions and points me towards a broader potentian of experiments :smile:


Burnout. Like Ferather said there is different type of rizos and the one you have seen lately is the same that I have had in my jars. I wasn't happy to find out it was signals of a stressed mycelium but I learned about different structures of rizo and different rizo triggers. One always learns something when one rattles the book case :smile:


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OfflineFerather
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Re: Cultivation General Discussion [Re: FishLevelMidnight]
    #24810257 - 11/27/17 06:01 PM (6 years, 2 months ago)

@00Burnout, it's interesting to see, but ultimately not good. It doesn't colonize properly at all.

I've seen recent images of cyans on grain + wood chips, looks healthy and like oysters.
Then I've seen images of nothing but "root", and terrible colonization.

----

Also CO2 is a funny thing, as anaerobes feed using it. The difference being mycelium use O2 to decay materials.
So low O2 will reduce decay and produce "low nutrient" settings, and then contamination from anaerobes.

There is no way to stop mycelium from expelling CO2, but, the CO2 can be converted.
Unfortunately that much CaCO3 in a substrate may be harmful, not tested it.

CaCO3 converts to calcium bicarbonate (soluble) when mixed with CO2.
Mycelium absorb the calcium bicarbonate, and utilize it.

----

Yes mycelium accedentaly-on purpose feed anaerobes.


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OfflineFerather
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Re: Cultivation General Discussion [Re: Ferather]
    #24810291 - 11/27/17 06:27 PM (6 years, 2 months ago)

Evasive rhizomorphs can also happen due to inhibitory materials in a plant such as wood, too much of an element, a toxic element, unusable build up, so on.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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InvisibleGerms
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Re: Cultivation General Discussion [Re: Ferather]
    #24810370 - 11/27/17 07:13 PM (6 years, 2 months ago)


Gonna test this stuff out with pans and cubes, maybe some bottle grows too


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Germs]
    #24812085 - 11/28/17 02:47 PM (6 years, 2 months ago)

so ive been having contam issues... that i can not shake. it seems to only happen at inoculation.
something gets into my jars when i open them:confused: how do you guys open your jars?

i havent given it much thought but i use the rubber side down(some jars i removed the rubber..)
and i have to almost pry them open. im thinking this is where something sneaks inside.

only recently did i notice this since i bought a few boxes of jars and i think i botched everything with sloppy openings.:facepalm:

older jars have been used a million times and the seal doesnt do shit. should i flip my lids?


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: mushboy]
    #24812101 - 11/28/17 02:52 PM (6 years, 2 months ago)

that's pretty likely. It won't hurt to try. I wish that was my case, but I think it's either my grain prep or my culture, can't tell wich


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24812108 - 11/28/17 02:55 PM (6 years, 2 months ago)

my shit only craps out when i pour(g2g or pouring lc). agar to grain is no problem cause i guess my approach is better with 'cracking' the lid.

when you pour, you kinda gotta do more then crack the lid. and with my shaky hands it kinda fucks me.


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Re: Cultivation General Discussion [Re: mushboy]
    #24812118 - 11/28/17 02:59 PM (6 years, 2 months ago)

Why not add another hole to the lid, with a plug. Big enough to put your sample in the jar.
If the plug stops air, it might be small enough that it's better than opening the lid.

You can spray and tape over the plug, to seal it when you are done.

:shrug:


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Ferather]
    #24812130 - 11/28/17 03:08 PM (6 years, 2 months ago)

nobody else on here uses hatches for g2g. either my opening of the jars is fucked or something with my technique.
im asking how you guys open your jars. ive seen the videos but im just wondering if im overlooking anything.

so detail some steps my homo sapien.


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InvisibleJosex
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24812151 - 11/28/17 03:18 PM (6 years, 2 months ago)

Quote:

tryptkaloids said:
that's pretty likely. It won't hurt to try. I wish that was my case, but I think it's either my grain prep or my culture, can't tell wich



Have you considered the possibility of being neither? You gotta be open to eveeything and observe. You've been around a while and I'd like to think that by now you know how to do a proper prep or how to spot a sketchy culture, besides I doubt you only have one culture, right? And pretty sure you've been trying hard to get your prep right.

What about switching grains? I can tell you from experience that sometimes grains are so fucking dirty with endos that they'll rot with bacteria no matter how you prep them. Also, bacteria coming from grains is damn easy to identify.

Another thing you can do is sterilize the shit out of dirty grains, like 3 hours at 20psi for quart jars. The grains will dry out some and that can slow down colonization a lot, but if you use liquid inoculant like LC colonization will be pretty fast and it'll balance out the moisture content. Even if you do a bad prep which I doubt, extended pc times at high psi will take care of it.


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Josex]
    #24812216 - 11/28/17 04:07 PM (6 years, 2 months ago)

My last run I did a few weeks ago is all molded.

Seriously about to stop retrying time and time again. I'm a mold farmer.


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Re: Cultivation General Discussion [Re: Moabfighter]
    #24812220 - 11/28/17 04:10 PM (6 years, 2 months ago)

Start fresh it is truly one of the easiest fixes...new lids new spores fresh plates


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OfflineFerather
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24812221 - 11/28/17 04:11 PM (6 years, 2 months ago)

Oh right G2G thought you meant agar to grain.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Ferather]
    #24812274 - 11/28/17 04:35 PM (6 years, 2 months ago)

im only farming mold when i go to expand my grains. maybe my spawn isnt as clean as i think? but i can drop wedges all day, no issues.

something about fully opening/removing the lids. that critical moment where the lid goes from cracked to fully opened.
...something is getting inside.:oldman:


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