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OfflineFerather
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Re: Cultivation General Discussion [Re: Ferather]
    #24805454 - 11/25/17 11:11 AM (6 years, 2 months ago)

Cubensis dry spores to WL-Tek @ 1.2% nitrogen + compost, pH 6.0-6.5. 48 hours after the sugar drop, it has grown.
However based on 48 hours it's likely it already germinated, but was slow-weak, sugar amplified decay.

It's pushing out faster than 2.4% nitrogen @ pH 7.0-7.5, both using the same water content.
Same interesting result with the birch wood peg, the cubensis seems to like it



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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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Offline00Burnout
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Re: Cultivation General Discussion [Re: Ferather]
    #24805523 - 11/25/17 11:44 AM (6 years, 2 months ago)

Does this look right to you guys? Some of the grains appear to have a dusty look to them, I think it might be white mold. If that's the case I'm gonna kick myself.


--------------------
Peace, pot and microdot!:mushroom2:
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Offlinehamloaf
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24805525 - 11/25/17 11:45 AM (6 years, 2 months ago)

That's ready for a shake.  How the myce recovers from the shake will tell the tale of whether or not the myce is contaminated.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: hamloaf]
    #24805537 - 11/25/17 11:50 AM (6 years, 2 months ago)

:whathesaid: take a pic 24 hours after a shake and  then we can tell you what to do with it


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
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Offline00Burnout
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Re: Cultivation General Discussion [Re: hamloaf]
    #24805540 - 11/25/17 11:53 AM (6 years, 2 months ago)

I gave them all a shake today, except for one that got shook yesterday. Here's how that one looks roughly 14-16 hours after the shake.

I did a mini mono with this culture, using brf for spawn, at the end of october that stalled on me and eventually greened out so I'm a little nervous about this one.


--------------------
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No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offlinehamloaf
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24805550 - 11/25/17 11:56 AM (6 years, 2 months ago)

Looks good so far, brother.  Be patient and keep an eye on it.  Give it a few more days.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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Offline00Burnout
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Re: Cultivation General Discussion [Re: hamloaf]
    #24805569 - 11/25/17 12:03 PM (6 years, 2 months ago)

Thanks, I just needed some peace of mind. Oats and agar are cheap, but time isn't. Sadly I don't have any more qt jars to get some of my more promising cultures started on...I need to slow down on the agar work lol. I've got a gt clone, 3 gt ms plates, and 4 purple mystic plates all ready for grain.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24805574 - 11/25/17 12:05 PM (6 years, 2 months ago)

that's good recovery time. That's good to spawn for sure. not too sure about G2G though


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineFerather
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24805581 - 11/25/17 12:07 PM (6 years, 2 months ago)

Quote:

00Burnout said:
I gave them all a shake today, except for one that got shook yesterday. Here's how that one looks roughly 14-16 hours after the shake.

I did a mini mono with this culture, using brf for spawn, at the end of october that stalled on me and eventually greened out so I'm a little nervous about this one.




Good luck.

:thumbup:

----

Side note:

Germination has occurred with growth after 4-5 days, with dry spores, in "all" of my cubensis tests.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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Edited by Ferather (11/25/17 12:13 PM)


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Offline00Burnout
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24805599 - 11/25/17 12:13 PM (6 years, 2 months ago)

Quote:

tryptkaloids said:
that's good recovery time. That's good to spawn for sure. not too sure about G2G though



I'm not going to bother with g2g this time around, I'm more focused on getting a successful bulk grow before I start playing with new techniques like LCs, LIs, and g2g.

I need to get better at taking clones, I can't figure out why but every tissue sample I take gets this weird soupy growth that I assume is a mixture of mycelium and bacteria. It took me 6 transfers on my last clone to get anything that looked like healthy mycelium.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24805614 - 11/25/17 12:24 PM (6 years, 2 months ago)

it's definately bacteria. what are you doing to get tissue samples?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineFerather
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24805621 - 11/25/17 12:27 PM (6 years, 2 months ago)

Heavy rain effect, water and soluble nutrient uptake, and waste removal.

   

----

Old test, not ideal as a method:

Here is an old project where I sat a cake in water, it floated and fruited.
It actually did very well for 50% humidity, and horizontally.

   

   

That's about 10% colonized, lol.

----

2 year old cake sample, into CaCO3 + water (pH 8.8), with waste removal.
The mycelium also visibly recovered, and started to grow out.

   


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Edited by Ferather (11/25/17 12:33 PM)


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Offline00Burnout
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24805633 - 11/25/17 12:31 PM (6 years, 2 months ago)

Quote:

tryptkaloids said:
it's definately bacteria. what are you doing to get tissue samples?



Ripping open the stipe and taking a small piece of inner tissue near the base with a flame sterilized blade cooled in the receiving dish.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
Ghetto Greenhouse
Trade List
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OfflineFerather
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24805670 - 11/25/17 12:53 PM (6 years, 2 months ago)

If you like, try 1-2g of black tea leaves and 1g ME to 100g water, assemble as normal, and take a sample to that.
It will be slower than 2-3g ME agar, however it should help you make clean transfers, also try pegs.

The recipe is not perfect, but the tea leaves add nutrients and inhibitory effects.
The extra macro-micro nutrients will allow you to use less ME.

Even cubensis will transfer to a birch toothpick peg.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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Offline00Burnout
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Re: Cultivation General Discussion [Re: Ferather]
    #24805712 - 11/25/17 01:18 PM (6 years, 2 months ago)

I might try that, I'll have to go get some though. All I have is oolong and raspberry green tea. I've thought about pouring another layer over it to let the mycelium grow uo through it and leave the bacteria behind.

Like I said previously though, I have plenty of clean cultures to keep me busy for awhile, so this clone isn't very high on my priorities.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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OfflineFerather
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Re: Cultivation General Discussion [Re: Ferather]
    #24805721 - 11/25/17 01:26 PM (6 years, 2 months ago)

Fruiting settings, my notes:

----

Heavy rain:

I have already mentioned heavy rain and substrate drainage. Ideal for all mycelium, also triggers fruiting.

----

Humidity:

Higher amounts lower the rate of water loss, however higher amounts also lower the ratio of other components in the air.
When maximum water capacity (100% humidity) is achieved the total amount of O2 (oxygen) as percent, lowers.

Mycelium utilize O2, to decay medias (oxidization), which can also kill anaerobes (CO2 loving).

----

Misting:

Usually adds clean plain water directly to the surface of a colony and-or fruit bodies. Mimics light rain fall.
Not as effective as heavy rain, however it has the same basic benefits in a much lower amount.

Can cause pathogenic aerobes and facultative anaerobes to germinate on fruits-other.
No notable contamination with pH 8.8 calcium bicarbonate water "so far".

----

Temperature:

Usually based on the mycelium, since the mycelium we cultivate can colonize a substrate at 22-24°C.
Most likely set by the genetic response to water uptake and replacement, vs, water loss.

The water lost to air (evaporation) is much slower when its cold.

----


Note:

Wood ash, if cheap or free, will be beneficial, it has a high pH (CaO).
The ash also contains lots of elemental oxides (oxygen).

Both wood ash and CaCO3 can trigger fruiting.

----

You can research light, correct anything wrong, thanks.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflineFerather
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24805780 - 11/25/17 01:55 PM (6 years, 2 months ago)

Quote:

00Burnout said:
I might try that, I'll have to go get some though. All I have is oolong and raspberry green tea. I've thought about pouring another layer over it to let the mycelium grow uo through it and leave the bacteria behind.

Like I said previously though, I have plenty of clean cultures to keep me busy for awhile, so this clone isn't very high on my priorities.




There is something else you can do, but I never fully tested it, as I used a fridge for 12°C. But you can try a pH wash.
I put my wild sample into CaCO3 water, pH 8.8, it stored for over 4 months. The sample grew as normal.


The settings:

> Submerging in fluids cuts of O2, preventing tissue decay, and culling aerobes.
> The higher pH and calcium itself, will kill "intolerant" organisms.
> Transfer to high O2, normal pH, will generate a switch.


   


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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Invisibleeatyualive
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Re: Cultivation General Discussion [Re: Ferather] * 1
    #24805792 - 11/25/17 02:00 PM (6 years, 2 months ago)

I've floated subs in the past and had great results. In fact I gave a substrate to a buddy long ago. He threw it in the yard. It flooded for 3 days. The sub was completely submerged for that time. He calls me about 2 days after the flood and I go over and see a full canopy explosion with the sub floating on 3 inches of water.

On another note, I used to pick fruits from this field that would flood during a heavy rain. One day I went out and was two foot deep in water about halfway through the field. So i go back 2 days later and cubes and pans everywhere. The dung piles were completely under water for a few days. Then they would explode about two days later! Nature is cool.


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InvisibleTookitooki
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Re: Cultivation General Discussion [Re: Ferather]
    #24805799 - 11/25/17 02:03 PM (6 years, 2 months ago)

What effects the speed of spawn colonization more?  I read people can colonize jars in 5-8 days.  I can only come close to that when I run lc,. And that's putting 10cc plus in each jar.    I'll drop a whole plate of agar in a jar and I still get an average of 12-16 days or more.    Doesn't seem to matter what culture I run.  My PE clone is the only one that is slower then the rest.  Would love to shorten my colonization times.


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InvisibleMunchauzen
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Re: Cultivation General Discussion [Re: Tookitooki] * 1
    #24805826 - 11/25/17 02:16 PM (6 years, 2 months ago)

Quote:

Tookitooki said:
What effects the speed of spawn colonization more?  I read people can colonize jars in 5-8 days.  I can only come close to that when I run lc,. And that's putting 10cc plus in each jar.    I'll drop a whole plate of agar in a jar and I still get an average of 12-16 days or more.    Doesn't seem to matter what culture I run.  My PE clone is the only one that is slower then the rest.  Would love to shorten my colonization times.



its the inoculation method, for sure. from slowest to fastest:

- spores
- agar wedges
- tiger drop (whole agar puck)
- G2G
- LI
- LC
??- slurry?? 

also make sure you shake your lis, lcs, and slurries after you inoculate with them. the key to fastest growth is to have the whole jar colonize as one by evenly coating all the grains.

I'm not terribly experienced or well red with slurries, but iirc, they are very fast. somebody correct me if im off base.


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