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Invisibleweetsie
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Re: Cultivation General Discussion [Re: Kenetic]
    #23538715 - 08/13/16 06:52 PM (7 years, 7 months ago)

Quote:

kenetic said:
I just got back in from a month break and I've put in about 15 hours this week.  It's all worth it in the end.

Cool thread.

What's a good way to test isolates without using a bunch of material?  I've think the pf tek sounds great but how can I inoculate them with an agar culture, directly or indirectly?  The verm barrier is a problem.




If it's not too thick you can just suck up agar into a syringe. Munchauzen has a tek that involves squirting water onto the plate and sucking that up with fragments of agar.


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Active grow logs: Oysters on Straw Pellets
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Offlinelukehighwalker710
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Re: Cultivation General Discussion [Re: weetsie]
    #23538792 - 08/13/16 07:27 PM (7 years, 7 months ago)

Munch also has the blenderess Li tek in which you could use one plate to nocc up multiple jars.

I've never done pf tek but a good mini spawn to bulk application I plan to use to test isolates is Sloppy's double gulp tek. Its worked great for me so far with MS!


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:vaped: Highwalker :bongload:

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OfflineKenetic
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Re: Cultivation General Discussion [Re: lukehighwalker710]
    #23538850 - 08/13/16 07:51 PM (7 years, 7 months ago)

Yeah but I'm not going for bulk I"m actually just trying to test the most amount of isolates with the least amount of substrate or other materials.


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

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Offlinelukehighwalker710
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Re: Cultivation General Discussion [Re: Kenetic]
    #23538880 - 08/13/16 08:03 PM (7 years, 7 months ago)

Well if you plan to continue growing via pf tek, by all means test out your isolates with pf cakes. However, if you plan to use those isolates primarily on grains, I would recommend testing them on your grain of choice.

Isolates still have preferences, and may fruit better via one tek or another. If you want to test out a grain without spawning to bulk, case a tray.


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:vaped: Highwalker :bongload:

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InvisibleLocN9ne
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Re: Cultivation General Discussion [Re: lukehighwalker710]
    #23538933 - 08/13/16 08:32 PM (7 years, 7 months ago)

Alright, when you say "isolates" what exactly do you mean?


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Q&A
US vs. THEM

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OfflineKenetic
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Re: Cultivation General Discussion [Re: lukehighwalker710]
    #23538946 - 08/13/16 08:42 PM (7 years, 7 months ago)

I see your point.  But I think I could get a general idea with the p.f. tek and then have similar results with my bulk of choice.  I use rye and like bulk but I don't want to use it until I have the culture I want.  I'm done with m.s syringes and rye grain clones and unpredictable, contaminate-prone results. 
The water agar method seems like it could work but it exposes the water to the entire surface area of the petri dish and could easily mix with an unseen contam. 
Maybe I could sub the dry verm with a filter disk or just add some sterilized verm on top of the p.f. jars (sterilized w/o the verm barrier) after inoculation. 
I've heard of people using p.f. to test isolates so there has to be a good way to do it.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

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Invisibleweetsie
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Re: Cultivation General Discussion [Re: Kenetic]
    #23538962 - 08/13/16 08:53 PM (7 years, 7 months ago)

Yeah don't bother with dry verm if you have filter disks, that shits for unequipped noobs.

I would'nt do BRF cakes myself, complete pain in the ass to make and slow as you can't shake em.

I just make 1 grain jar with a wedge and spawn it at 50% to something easy like coir or straw pellets where you can just dump hot water on them and call it that. For containers I use jars, ice cream tubs, pyrex baking dishes, plastic vegetable trays, anti static bags.. basically the first thing that comes to hand when a jar reaches 100%. Wrap it in pvc food wrap and stab a few holes for GE. If you're testing and comparing isolates then obviously you wanna use something consistent, maybe buy a pack of take away containers or something.

If you're using a filter disk on your brf jars theres no reason why you couldn't move the sub to the side with a flamed scalpel and push a wedge down there.


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Active grow logs: Oysters on Straw Pellets
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OfflineKenetic
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Re: Cultivation General Discussion [Re: LocN9ne]
    #23538968 - 08/13/16 08:56 PM (7 years, 7 months ago)

Quote:

LocN9ne said:
Alright, when you say "isolates" what exactly do you mean?




I mean that I intend to take my spore prints from my last grow and first, isolate as many desirable growths away from contaminates as possible and then isolate the sub-strains away from each other until I get a bunch of monocultures.  Then I would like to fruit each monoculture and compare the results to determine which ones(s) to keep. 
Isolating from a clone would be easier but I think I could get better results if I isolate monocultures directly from multispore before they have a chance to mix with each other. 

Just for the hell of it.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

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InvisibleMad Season
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Re: Cultivation General Discussion [Re: Kenetic]
    #23539315 - 08/13/16 11:21 PM (7 years, 7 months ago)

Pin on agar? Lol. If it does pin.. the fact it pinned on agar would show it's pretty baws atm


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InvisibleSupalemonhaze
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Re: Cultivation General Discussion [Re: Mad Season] * 1
    #23539645 - 08/14/16 02:06 AM (7 years, 7 months ago)

Quote:

kenetic said:
I just got back in from a month break and I've put in about 15 hours this week.  It's all worth it in the end.

Cool thread.

What's a good way to test isolates without using a bunch of material?  I've think the pf tek sounds great but how can I inoculate them with an agar culture, directly or indirectly?  The verm barrier is a problem.




If you are planning on using the best culture on bulk in the future, you should test it out on bulk, not cakes. One strain can do great on cakes but performs less on bulk and vice versa.

If I were you, I would do a single quart of each culture and spawn that quart to a tray of your favourite bulk. Then, fill monos with these trays and put into fruiting. I've been doing nothing but trays lately, too damn easy to inspect and harvest. I fit 4 trays in a single mono so with 10 isolates, I can test them all side by side and all at once using no more than 3 monos.

I especially like trays because I had too many mushrooms to know what to do with like, months ago. This way, I can have multiple projects going on at the same time, taking less space and eventually, having less mushrooms just sitting there in storage. Just as much fun with a fraction of the baggage.

Another great alternative is Sloppyjaw's big gulp tek. You should take a look at that for sure.

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Invisibled0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
Re: Cultivation General Discussion [Re: Supalemonhaze]
    #23539708 - 08/14/16 02:29 AM (7 years, 7 months ago)

Quote:

Supalemonhaze said:
If you are planning on using the best culture on bulk in the future, you should test it out on bulk, not cakes. One strain can do great on cakes but performs less on bulk and vice versa.





This.


Also I would add to use the same sub for testing clones/isolates that you plan to use in the future. ie coir vs hpoo etc

e.g.  If you isolate a clone on hpoo, but them fruit it on coir on a larger scale later, you probably won't get as good of yields.


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OfflineFractaliopsybe
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Re: Cultivation General Discussion [Re: Inocuole]
    #23539739 - 08/14/16 02:49 AM (7 years, 7 months ago)

Quote:

Inocuole said:
Don't know why we don't have one of these, but apparently people get fussy when posts are "off topic" in a given thread.  So, here's a thread where nothing is off topic as long as you can somehow draw a connection between it and cultivation.
I don't have any major topic suggestions, but if you're looking for something worth talking about, you could start with genetics, lineages, site/community project suggestions, "dafuq is this?" type posts, requests for assistance pointing to your own thread, disputing current techniques, etc.
So, if that's all, and I think it is, go ahead and cut loose.




Lol, Wish this was known to me the other day...lol (then again I was a bit lost&:crazy2:) :facepalm3:
Sincerely apologetic of my Causations & annoyances in that matter.^
-------------------------------
Shalom Everyone. 

Does any know of any1 Fruiting P.subs with the help of a fridge?

Alloy tray with glad wrap atop, Trialling 8-16hrs in 5c* (RH?-TBA) then take out and put in 13c @ 80-94RH tub and play with the "massaging" of temp changes in and close to out the right conditions. Is what I'm hoping will help boost it so I can converge this in to a consistent indoor TEK, Applied with to double layer SGFC tubs or bucket.

Which is in proper trials now, So I will keep the TEK that I'm attempting in consolidation and Break it down before releasing and can wait to present successful results* (finger-crossed)

I've had some success already.. just not that well..  As few pins pop up but has a 50/50 chance of Growing or aborting.

*hints my trials of this as, Lots of people just do YEARLY/ season woodlover grows from what ive seen. but none are indoor.
:elmo: Wish me luck and Suggestions are appreciated.
:excellent:


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Psilocybe Cubensis.:mushroom2:Panaeolus Cyanescens.:mushroom2:Psilocybe Subaeruginosa. Dimethyltryptamine.:deemsters:

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OfflineKenetic
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Re: Cultivation General Discussion [Re: Supalemonhaze]
    #23540321 - 08/14/16 10:05 AM (7 years, 7 months ago)

That's a great idea.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

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Offlinewtfcrazymofo
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Re: Cultivation General Discussion [Re: azur]
    #23540534 - 08/14/16 11:21 AM (7 years, 7 months ago)

Quote:

azur said:
Here's a tek, read a book





--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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OfflineFractaliopsybe
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Re: Cultivation General Discussion [Re: wtfcrazymofo]
    #23542148 - 08/14/16 08:42 PM (7 years, 7 months ago)

Quote:

kenetic said:
That's a great idea.



Is that to me?

Quote:

azur said:
Here's a tek, read a book





Dafuq is that shit, lol... :eek:

Edited by Fractaliopsybe (08/15/16 03:23 AM)

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InvisibleSupalemonhaze
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Re: Cultivation General Discussion [Re: Fractaliopsybe]
    #23542804 - 08/15/16 02:26 AM (7 years, 7 months ago)

Is it? I never really got that around here.

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OfflineKenetic
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Re: Cultivation General Discussion [Re: Fractaliopsybe]
    #23542960 - 08/15/16 05:28 AM (7 years, 7 months ago)

Quote:

Fractaliopsybe said:
Quote:

kenetic said:
That's a great idea.



Is that to me?

Quote:

azur said:
Here's a tek, read a book





Dafuq is that shit, lol... :eek:




No it was to sup (see the header of my post).  Not to stay your idea isn't good either lol


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

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Offlinewtfcrazymofo
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Re: Cultivation General Discussion [Re: Inocuole]
    #23543017 - 08/15/16 06:24 AM (7 years, 7 months ago)

Us mycology cultivaters... why do we do it?


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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Invisibleblindingleaf
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Re: Cultivation General Discussion [Re: wtfcrazymofo]
    #23543083 - 08/15/16 07:10 AM (7 years, 7 months ago)

Quote:

wtfcrazymofo said:
Us mycology cultivaters... why do we do it?






--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: blindingleaf]
    #23543092 - 08/15/16 07:17 AM (7 years, 7 months ago)

pc'ing jars now. Only holds 3 quarts at a time and I made 6. Jiggler just started rocking at 9, so gonna let them go until 10:30, then take them off and shake. Do the other 3 after that. Shake. Go to work until 10, maybe 11. They should be cool by then to inoculate yeah?


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KSSS And PE WBS.
 

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