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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Quote:
TheMadHatter420 said: Dude welcome to 2017 where most high school graduates don't even know how to hard boil an egg. Hell we elected Trump as president. That should tell you enough right there, lol.
And this is why I don't play well with others in mericas everyday society. They never disappoint when it comes to making me confirm why I don't socialize much as I get older. Idiocracy really is going from a movie to a documentary like the cover says. electrolytes its what plants crave
-------------------- Keep your feet on the ground and your head in the clouds
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Sad but true!
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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I just assembled another tea bag, but for oyster, I cut off the bald end of the peg. As I put it on the shelf, I noticed there is mycelium "inside" the peg.
The peg is still solid, needing cutters to cut it.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Ferather] 1
#24802004 - 11/23/17 10:47 AM (6 years, 2 months ago) |
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That thread probably would have been gone earlier if everyone told a mod instead of put their 2 cents in lol
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Pipefitter537
You're Not Yelping



Registered: 11/25/16
Posts: 715
Loc: SodoSopa
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Re: Cultivation General Discussion [Re: bodhisatta]
#24802016 - 11/23/17 10:56 AM (6 years, 2 months ago) |
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Hit the whistle and it said there was already a report ongoing
-------------------- "You should never underestimate the predictability of stupidity"-Bullet Tooth Tony
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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And it's hard not to blast someone that's such a dumb fucker after they make a post like that.
-------------------- Keep your feet on the ground and your head in the clouds
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: bodhisatta]
#24802025 - 11/23/17 11:03 AM (6 years, 2 months ago) |
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Quote:
bodhisatta said: That thread probably would have been gone earlier if everyone told a mod instead of put their 2 cents in lol
I told a mod 6 min after it was posted and had no responses, even tagged it as important. BUT it is a holiday.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 11 hours
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Re: Cultivation General Discussion [Re: bodhisatta]
#24802037 - 11/23/17 11:11 AM (6 years, 2 months ago) |
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Quote:
bodhisatta said: That thread probably would have been gone earlier if everyone told a mod instead of put their 2 cents in lol
now I wanna see the blast people put this fucker on. I'm guessing the thread was taken down and not just locked huh?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24802322 - 11/23/17 01:46 PM (6 years, 2 months ago) |
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Well my cubensis test confirms acidity is required for cubensis, its cellulase enzyme(s) function at pH 7.0-7.5, but slowly. At pH 6.0-6.5 they function at relatively normal speed, and feeding is normal, no stalling or issues of any kind.
This near enough concludes the cellulase produced by cubensis is "hybrid" which works at pH 4.5-7.0. Out of the 4 types of cellulase hybrid has the largest working range, an allowance of 2.5.
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When mycelium (even from spawn) colonizes a substrate, over time it becomes more acidic. As the acidity rises, the usefulness of amylase (starch), begins to fall off the table.
At the same time the hybrid cellulase enzyme becomes more effective. Suddenly the nutritional spawn, is no longer nutritional.
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Since amylase becomes less effective, the nutrients locked up in the spawn, will likely go unused. The calculation for 50:50 does not take entire effect, cold cellulose makes it even worse.
Since the nutrients are now locked up by pH, the mycelium must relay on cellulose. If the cellulose base, where cold or weak, the mycelium will struggle.
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It's entirely possible to birth mycelium on a cellulose base, enriched with macro-micro nutrients. In the case of cubensis (no or limited laccase), a basic starter carbon is essential.
In nature the decay of animals-other would be enough to jump start cubensis. For lignicolous mycelium add phenol's, such as tea or wood extract.
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Due to no or limited laccase, cubensis is unable to utilize relevant materials (in place of sugars), and they are usually inhibitory. This means the cubensis cannot, or should not, germinate nor grow on wood, unless modified like in my tests.
However, cubensis do oxidize materials, but not with laccase (or very little).
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Also cubensis likes lots of macro-micro nutrients with it's carbon.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Ferather]
#24802360 - 11/23/17 02:12 PM (6 years, 2 months ago) |
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Some notes on coir (I am not bias, just be aware):
Coir has a pH of 5.5 to 6.8, which is less than neutral (7.0), therefor it can germinate mold.
At the same time, being acidic means anything that germinates will be more vigorous. This is due to the cellulose, and the acidity requirement of it's cellulase.
Plus mold is lignicolous and uses laccase, as normal.
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Additional notes:
α-Amylase (the most effective starch enzyme), works best at pH 6.7 - 7.0. This means coir can be already out of range, or bordering it.
Cellulase, and β-Amylase (less effective) will take over.
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Cautions:
Enrichment via solubles, as in WL-Tek, will likely cause contamination when exposed. However, it should be possible to increase its pH to 7-7.5, and try then.
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Paper pellets are roughly pH 7.2, and void of most essential nutrients. This is why I suggest using paper as cellulose, for general use.
100g of paper pellets can hold up to 375g of water.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Ferather]
#24802387 - 11/23/17 02:37 PM (6 years, 2 months ago) |
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No what happens to the PH in the coir, when mixed, if my tap water is in the 8-8.5ph range? Would it bring the PH up into the acceptable range during hydration of the coir? With soil and plants it takes a few watering's with adjusted PH to get things into line.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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That's a bonus, hard water is a great source for live mycelium (I also have hard water), it does bring up the pH, but the coir varies. So I can't say, you will need to get strips, or better yet a digital pH pen (example), wait till cool (room), then test.
You could make a small sample, say 10g, hydrate it, test it. Add CaCO3 mix and repeat the test. Measure how much CaCO3 powder that was used for 10g of coir, and work with that.
You may need to test each new batch, as mentioned the pH varies. Also check the water is calcium based not sodium based.
Edited by Ferather (11/23/17 02:52 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Ferather]
#24802424 - 11/23/17 02:56 PM (6 years, 2 months ago) |
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All i know is coir is reptile bedding and nothing germinates on it for weeks/months
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: bodhisatta]
#24802427 - 11/23/17 02:57 PM (6 years, 2 months ago) |
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Quote:
bodhisatta said: All i know is coir is reptile bedding and nothing germinates on it for weeks/months
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Ferather]
#24802432 - 11/23/17 02:58 PM (6 years, 2 months ago) |
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I miss my PH meter. I need to buy a new one soon.
EDIT
I would assume the waste products from those reptiles would increase the acidity of the bedding. I could be wrong.
Not sure why it is, but the water PH of this town and one 5 mile south is high. Like 8-9 range. I lived in the south town when growing bud and had to adjust the PH down like I do here for potted plants indoors.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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The handheld sticks are usually pretty shit even ones that cost 100$
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: bodhisatta]
#24802442 - 11/23/17 03:05 PM (6 years, 2 months ago) |
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IME they are usually not to far off, if they are in calibration. I usually try to shoot for middle of the PH range for this reason. In Hort. class we played around with the Cheap stick ones and they would always be within .1-.2 of the teachers nice probe model. She wouldn't let us play with that one. It was nice as fuck, and I want one like it but done feel like dropping a couple bills on one.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Probably because it was over $300 and the probes that only last a year well maintained cost 80+
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: bodhisatta]
#24802452 - 11/23/17 03:08 PM (6 years, 2 months ago) |
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O I know. I was looking at them long ago. I find for plants in soil, the cheap ones work fine as you have the soil/soilless mix as a PH buffer. Things don't swing as fast in soil as they do in Hydro.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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I worked at this brewery that we all had these Milwaukee tool brand handhelds theyre like $120 at the time. Freshly Calibrated in 7 and 4 you could take three of them and put them in a sample and they all read 0.05-0.1 difference. Which is fine for most people. But you would think by now for that kind of coin shit would work better
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