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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 15 hours
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Re: Cultivation General Discussion [Re: mushboy]
#24799701 - 11/22/17 10:32 AM (6 years, 2 months ago) |
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Quote:
Josex said:
 Duuuuuuuuuuuds! I have a wiener here! This is KSSS, not even half way colonized and the bitch is knotting, never seen anything like this. This is just one transfer away from the streaked plate using the poke.
that tells me there's bacteria hiding in the rest of the plate...
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: mushboy]
#24799702 - 11/22/17 10:33 AM (6 years, 2 months ago) |
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Quote:
mushboy said: I dont spray my box at all anymore.
I started being 'sloppy'and didnt spray a few sessions. Now i dont spray at all.
True story
I did that too. I've gone back to spraying until I know where my bacteria is coming from though
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: tryptkaloids] 1
#24799758 - 11/22/17 10:55 AM (6 years, 2 months ago) |
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Ime bacteria comes from spores, dirty tissue or bad grains(prep). Misting/not misting your SAB isnt causing or preventing bacteria
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: mushboy]
#24799763 - 11/22/17 10:59 AM (6 years, 2 months ago) |
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I would think a negative ion generator like Josex and natedognow suggested would do a much better job of sinking floating particles like spores and dust than spraying water will
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Germs]
#24799768 - 11/22/17 11:00 AM (6 years, 2 months ago) |
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Still air makes gravity work on small particles pretty effectively
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#24799805 - 11/22/17 11:18 AM (6 years, 2 months ago) |
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24799810 - 11/22/17 11:23 AM (6 years, 2 months ago) |
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Quote:
tryptkaloids said:
Quote:
Josex said:
 Duuuuuuuuuuuds! I have a wiener here! This is KSSS, not even half way colonized and the bitch is knotting, never seen anything like this. This is just one transfer away from the streaked plate using the poke.
that tells me there's bacteria hiding in the rest of the plate...
Dude you talk with such authority sometimes... like no fucking shred of a doubt . Some phrases like 'I think', 'could be', would be nice additions to your posts. There's no bacteria there. Even if I could not tell visually, the method being using to transfer will make bacteria grow first on the plate before the myc starts growing. I don't feel like explaining anything because it's already written somewhere.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Last seen: 3 days, 15 hours
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Re: Cultivation General Discussion [Re: Josex]
#24799824 - 11/22/17 11:32 AM (6 years, 2 months ago) |
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Quote:
mushboy said:

Ime bacteria comes from spores, dirty tissue or bad grains(prep). Misting/not misting your SAB isnt causing or preventing bacteria
I agree, but the less variables for me to narrow down the better. Quote:
Josex said:
Quote:
tryptkaloids said:
Quote:
Josex said:
 Duuuuuuuuuuuds! I have a wiener here! This is KSSS, not even half way colonized and the bitch is knotting, never seen anything like this. This is just one transfer away from the streaked plate using the poke.
that tells me there's bacteria hiding in the rest of the plate...
Dude you talk with such authority sometimes... like no fucking shred of a doubt . Some phrases like 'I think', 'could be', would be nice additions to your posts.
you are 100% right about that. I'll start working on that.
Quote:
Josex said:There's no bacteria there. Even if I could not tell visually, the method being using to transfer will make bacteria grow first on the plate before the myc starts growing. I don't feel like explaining anything because it's already written somewhere.
it just doesn't make sense to me for myc to switch it's energy from colonization to fruiting before it has access to every bit of energy possible. from an efficiency standpoint wouldn't you want as much nutrition as you can get before moving on?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24799834 - 11/22/17 11:38 AM (6 years, 2 months ago) |
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It is not guaranteed anywhere that myc has to colonize 100% before fruiting. In nature, myc will never fully colonize its substrate seeing as that is impossible yet will fruit just fine. 100% is just a good indicator of when fruiting will happen but I'm fairly sure that it's possible for it to pin without there being bacteria present. A thought not a theory
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: tryptkaloids] 1
#24799842 - 11/22/17 11:43 AM (6 years, 2 months ago) |
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It's unusual to get pins before 100% colonization but it happens.
At least for me, bacterial agar cultures have never pinned faster whatsoever.
Mycology is such a baby science yet and we have very little proof to back up some claims, but I have a gut feeling that that happens in those instances when bacteria gets such a foothold on part of the substrate that makes it impossible for myc to colonize, so myc thinks its job is done and starts to pin as a survival response. Very typical seeing that with cakes.
Edit: the survival response theory though, I was just parroting that and I don't know if I buy it. How about noobs waiting a long ass time to get those parts of the cake colonized? They never colonize and the colony starts pinning just because it has to.
Edited by Josex (11/22/17 12:02 PM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Josex]
#24799908 - 11/22/17 12:26 PM (6 years, 2 months ago) |
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I forgot to mention in my test, I live in a hard water area, not only does my water contain chlorine, but also calcium bicarbonate. The calcium bicarbonate, is the soluble end result (clear) of CaCO3 (white, insoluble) + CO2, which also has an effect.
My cold tap water has a pH of 7.5-7.8, the bicarbonate also reacts with acids (decay), useful for leeching.
In the pegs case, it should have absorbed the trace chlorine and bicarbonate.
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@natedawgnow, I agree, mycelium tend to focus more on fruiting than colonizing outdoors. There is also the case of other micro-organisms taking what the mycelium wants.
Mycelium-other is far more likely to populate an "optimal" substrate. Given that room temperature and regulation play a part.
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Try growing outdoors, in an area full of various "hungry" animals, and all you have is sticks.
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TheBlackCat
Possibly Human



Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: crowseed]
#24799928 - 11/22/17 12:33 PM (6 years, 2 months ago) |
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Quote:
crowseed said: Thanks all for SAB technique sharings. Sounds like I've been spraying mine too much. Black cat, coir in oyster buckets works well. Also, the babbies will just push the micropore out - no need to do anything.
Oh nice! I think I may just stick to sterilized supplemented bags this time around. I need my coir for my cubes.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24801639 - 11/23/17 07:07 AM (6 years, 2 months ago) |
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Here are today's images, plus enhanced images to get over the plastic, cubensis colonizing lignicolous materials. Normal speed and population, well fed, not weak, more active on mild acidity pH 6.0-6.5, sugar assisted.

Requires leeching and trace starter carbon (glucose, other sugars).
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Cultivation General Discussion [Re: Ferather]
#24801815 - 11/23/17 09:08 AM (6 years, 2 months ago) |
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Any mod see that bullshit post from a guy asking for pounds of mushrooms? Might wanna handle that dumbass and set him straight
-------------------- Keep your feet on the ground and your head in the clouds
Edited by Shineonyoucrazy (11/23/17 09:09 AM)
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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There is a report option at the bottom of the post. I all ready reported it. I am sure it is a Pig or a true fuck-tard.
-------------------- JOIN THE POW WOW
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 15 hours
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cops aren't that dumb... atleast not the ones they have handling drug busts
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Cool thanks hatter. Never had to report anything, but good to know. How blatantly and utterly fucking stupid can someone be. I can't comprehend how someone can go on a page clearly label MUSH CULT and ask for steady pounds lol if its not leo its someone who ate paint chips while being dropped on their head repeatedly as a child
-------------------- Keep your feet on the ground and your head in the clouds
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Dude welcome to 2017 where most high school graduates don't even know how to hard boil an egg. Hell we elected Trump as president. That should tell you enough right there, lol.
-------------------- JOIN THE POW WOW
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 15 hours
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a friend from work and I were talking about the average reading level of presidents and how trump is the lowest and lincoln was the highest, I used to think we lost democracy completely but if you think about the average reading level of americans it lines up with our elected officials. maybe democracy is still alive in america
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Quote:
TheMadHatter420 said: Dude welcome to 2017 where most high school graduates don't even know how to hard boil an egg. Hell we elected Trump as president. That should tell you enough right there, lol.
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