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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: mushboy]
#24782234 - 11/14/17 10:38 AM (6 years, 2 months ago) |
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I got one for you ferather since your pic describes you so well.
How fast does a saline solution 25% NaCL kill bacteria and mold spores?
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: van hatton] 1
#24782296 - 11/14/17 11:15 AM (6 years, 2 months ago) |
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Quote:
mushboy said: I hope most of us know food sources left out and exposed can/will mold.
Indeed, but that's because the food is-should be full of cellulose-starch-sugar, nitrogen (protein), and other.
If you add starch-sugar, nitrogen, and other, to a plain media such as vermiculite, things grow. If you add just sugar, no other nutrients, to vermiculite, not much happens, or it stalls.
You can throw pure carbon at it all day, not much will happen without nitrogen. I'm not saying all the other nutrients are not essential, because they are.
Cold substrates are classed as cold due to low nitrogen.
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That being said, substrates containg no soluble (or 100% complex) carbon, can be enriched. However, this must be done without adding a viable carbon source for spores.
This allows you to make a enriched low contamination substrate.
Paper enriched with macro-micro nutrients, no added carbon:

Plain paper with added sugar, no additives:

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Quote:
van hatton said: I got one for you ferather since your pic describes you so well.
How fast does a saline solution 25% NaCL kill bacteria and mold spores?
"It will depend on the type of microorganism but I doubt you will normally encounter many microorganisms that will be happy in saturated NaCl! (~26% w/v, ~5M)." -- Source
"5g/100g water kills most things in two days, 10g/100g 30 minutes... 10% salinity killed 80% of organisms..." -- Source
5-15 minutes @ 25%
Edited by Ferather (11/14/17 11:39 AM)
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Ferather]
#24782321 - 11/14/17 11:27 AM (6 years, 2 months ago) |
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your guess is better than mine.
Only reason I say 25% is because at 100°c 1ml of water can hold roughly .27-.28grams of salt
Just pondering life over here thanks.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: van hatton]
#24782333 - 11/14/17 11:34 AM (6 years, 2 months ago) |
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Is this going to be used on live mycelium? I've never tried this method on mycelium. I have tried 25% salt + boiling water to clean yeast from containers.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Ferather]
#24782348 - 11/14/17 11:44 AM (6 years, 2 months ago) |
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Probably not but I may put a 25% solution into a petri. Basically lme with salt. Going to open it in my basement and see if anything will grow. Highly doubtful but why not.
More tests are needed on my part.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: van hatton]
#24782354 - 11/14/17 11:47 AM (6 years, 2 months ago) |
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I see, interesting. You can also try adding a drop liquid seaweed to the agar surface, the bacteria is salt tolerant.
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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Re: Cultivation General Discussion [Re: van hatton]
#24782360 - 11/14/17 11:53 AM (6 years, 2 months ago) |
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Why are you doing this? Just for the sake of experimentation?
If you do get any growth, what then?
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Sake of experimentation if this and some other tests go well I may have something.
Also if something grows in 25% salt solution from my basement I'll question if I should buy this house lmao.
There are things that require high salt content to thrive. Doubtful they are in my basement.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (11/14/17 11:57 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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You could try to find the maximum tolerance for the mycelium, and see if this reduces the overall contamination chance, as an agar clean-up recipe.
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Here are some hobby containers, its a glass jar with plastic topper. I can modify the topper, or replace it with kitchen film or foil. Can be used as spawn jars, tall agar jars, or any other purpose, the glass is tapered, and can be used for drinking. I had better start eating (chocolate spread), I lost the topper from the first one I got.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Ferather]
#24782382 - 11/14/17 12:01 PM (6 years, 2 months ago) |
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That's a thought too would be good for innoculations of spores print or syringe. Especially with heavy bacterial prints.. hmm
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: van hatton] 1
#24782458 - 11/14/17 12:30 PM (6 years, 2 months ago) |
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Finally got the response I was looking for, it seems the sugar peg is mostly good for germinating. After sugar dropping my test sample with ~14% sucrose water, it has regenerated better.
Growth is about twice as lively than on the trace sugars in the peg. Still no contamination, just weak cubensis on a peg.

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Experiment, cubensis spawn on WL-Tek (enriched cellulose, no added carbon): Using 10% (dry-to-dry), brown rice spawn for speed and delivery.


Here I was testing basic storage in low O2, which worked for a while.

Turned to goo eventually, water works best.
Edited by Ferather (11/15/17 09:17 AM)
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TheBlackCat
Possibly Human



Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: Ferather]
#24783491 - 11/14/17 08:43 PM (6 years, 2 months ago) |
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So question. This jar has been colonizing since the 25th and I see no green on the outside. Does that mean it's good to go? All the other ones got contaminated sadly. Would you shoebox this? Just ignore any dark spots you see. Those are shadows. My cell phone sucks. My 18-55 lens is sucking at life right now and not working.



Edited by TheBlackCat (11/14/17 08:48 PM)
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24783500 - 11/14/17 08:46 PM (6 years, 2 months ago) |
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G2g it
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TheBlackCat
Possibly Human



Registered: 10/05/16
Posts: 2,819
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Re: Cultivation General Discussion [Re: Germs]
#24783527 - 11/14/17 08:54 PM (6 years, 2 months ago) |
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I want to but I'm so afraid. What if there is like the tiniest bit of contam hiding out in there. All my jars got sick except this one.
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TheBlackCat
Possibly Human



Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24783530 - 11/14/17 08:56 PM (6 years, 2 months ago) |
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And we are talking over 20 contaminated jars. So yeah. I'm paranoid. I kinda fucked this one up. My agar looks really nice though now that I cleaned everything. Not seeing any visible contams yet.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24783531 - 11/14/17 08:57 PM (6 years, 2 months ago) |
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20s not too bad
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24783532 - 11/14/17 08:57 PM (6 years, 2 months ago) |
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I’m that case G2G is safer.
If you spawn to shoebox and there is one speck of contam, the whole thing is ruined. If you g2g it and there is one speck of contam, you’ll probably get at least some good jars out of it.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Quote:
fishermansjc said: I’m that case G2G is safer.
If you spawn to shoebox and there is one speck of contam, the whole thing is ruined. If you g2g it and there is one speck of contam, you’ll probably get at least some good jars out of it. 
That's shitty logic no offense
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 25 days
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Re: Cultivation General Discussion [Re: cronicr]
#24783543 - 11/14/17 09:02 PM (6 years, 2 months ago) |
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Because if you have one speck of contam, in reality when you g2g you will spread it around enough to fuck up all receiving jars?
That is most likely I logiced, but at best you could get some clean ones
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TheBlackCat
Possibly Human



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Re: Cultivation General Discussion [Re: cronicr]
#24783552 - 11/14/17 09:04 PM (6 years, 2 months ago) |
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Quote:
cronicr said:
Quote:
fishermansjc said: I’m that case G2G is safer.
If you spawn to shoebox and there is one speck of contam, the whole thing is ruined. If you g2g it and there is one speck of contam, you’ll probably get at least some good jars out of it. 
That's shitty logic no offense
Completely agrees with you. I would end up breaking up the bits of mycelium and spores from the contam and spreading it to several jars. Then I can collect an extra 9 jars of contams. For fucks sake. There trying to ruin my life Cronicer. 
I think I'll smell it then shoe box it.
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