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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Inocuole]
#24763731 - 11/06/17 11:59 AM (6 years, 2 months ago) |
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23TH all the way
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Josex]
#24763740 - 11/06/17 12:02 PM (6 years, 2 months ago) |
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1 = one 1st = onest? No why does the 'st' suddenly make 'one' into 'fir'
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: bodhisatta]
#24763743 - 11/06/17 12:03 PM (6 years, 2 months ago) |
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Because
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Re: Cultivation General Discussion [Re: bodhisatta]
#24763745 - 11/06/17 12:04 PM (6 years, 2 months ago) |
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Damn that's a good point. The english language is such a confusing pile of shit.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: 00Burnout] 1
#24763761 - 11/06/17 12:13 PM (6 years, 2 months ago) |
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: cronicr]
#24763957 - 11/06/17 01:49 PM (6 years, 2 months ago) |
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Quote:
cronicr said: No need to lime substrates just casing layers

The science is, the higher pH is tolerable to live mycelium (in some cases, it improves growth), whilst spores are more restricted by the higher pH. It's not 100% fail proof, however in cases where the casing is known as "cold" (very low nitrogen), adding it causes no real concern.
If the casing had been enriched (by any means) with a nitrogen source, adding lime can cause germination. Casing mixes are usually coir or a blend of coir and vermiculite, chemical free paper or wood.
For cubensis you obviously don't want wood, however all of the listed items are "cold". The lignin content in coir, card, and wood unfortunately can attract mold.
This is because mold (in this case) is lignicolous, and likes lignin. Lignin-other, are acidic, this is the default for the spores.
Tip: Gypsum is "not" used for pH balancing.
Good luck.
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: Ferather]
#24764139 - 11/06/17 02:57 PM (6 years, 2 months ago) |
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How long over 160 is too long for pasteurizing?
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Ferather]
#24764144 - 11/06/17 02:59 PM (6 years, 2 months ago) |
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I plan on doing some experiments with fruiting chambers soon and i'm curious about finding out a good way of
measuring FAE in 20L mini mono's. Thus far I have come to notice that 2 inches of perlite on the
bottom provides with 90-95% humidity in the chamber even tho the RH is the room is 0%.
The first thing that came to my mind was that I could create smoke inside the
tub and watch it dissipate to be able to decide how FAE occurs.
What do you think guys/gurls? Let me hear your interesting
ideas that could perhaps work in this scenario.
Ferather, you like to experiment, do
you think smoke could work?

(I have no clue how you arrange your texts, have to practice more)
-------------------- Cakes inside Water Tub
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Mateja]
#24764197 - 11/06/17 03:15 PM (6 years, 2 months ago) |
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It's all about, how to say what you want, using specific wordage that produces a nice triangle. You can call that an organized pattern of wordage and format arrangement, or OCD.
Unless the smoke has high O2, it will probably suffocate mycelium. If it increases the CO2, that would promote anaerobes.
Are we talking wood smoke or chemical smoke?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Ferather]
#24764207 - 11/06/17 03:18 PM (6 years, 2 months ago) |
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so you add unnecessary words or delete other words to make it seem like its all shorter.

thats insanity. you should eat those shrooms you grew fella
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: mushboy]
#24764225 - 11/06/17 03:24 PM (6 years, 2 months ago) |
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lol. lol again!
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: Germs]
#24764242 - 11/06/17 03:30 PM (6 years, 2 months ago) |
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Quote:
Germs said: How long over 160 is too long for pasteurizing?
The matter at hand fellas
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Germs]
#24764251 - 11/06/17 03:32 PM (6 years, 2 months ago) |
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hhaha.. i think a few teks say no more than 10 min otherwise your screwed.
but similar to cooking, you got carryover heat from thermal mass or whatever. so even if you catch it and turn it down it can still go up in temp.
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: mushboy]
#24764256 - 11/06/17 03:33 PM (6 years, 2 months ago) |
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I’m seeing in older posts that some went up to 170 with no problem. It was like 160-165 for an hour tho.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: Germs]
#24764262 - 11/06/17 03:37 PM (6 years, 2 months ago) |
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Quote:
Germs said: How long over 160 is too long for pasteurizing?
Not really how long but how high...170 used to be pretty standard these days we aim no higher then 160 for better success but id say anything over ten mi utes above 170 is worth a do over
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Ferather]
#24764326 - 11/06/17 04:01 PM (6 years, 2 months ago) |
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Quote:
Ferather said: Unless the smoke has high O2, it will probably suffocate mycelium. If it increases the CO2, that would promote anaerobes.
Are we talking wood smoke or chemical smoke?
No mycelium, only field capacity substrate and perlite in the mini mono's and the humidity is good.
Remember I am experimenting with creating fruiting chambers and would like a method for measuring FAE since I am going to experiment with hole size and all that to dial them in.
Mostly I would like a method of measuring FAE that would be relevant both to perlite in mini mono and field capacity substrate.
Dry smoke will not behave the same as 95% humid air inside a monotub. So I am asking if anyone with some engineer background can chime in if he/she thinks he/she understand what I mean! 
Thanks guys
-------------------- Cakes inside Water Tub
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Mateja]
#24764358 - 11/06/17 04:14 PM (6 years, 2 months ago) |
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I see, sorry, I can only find anaerobe indicator test's, there are some readers but they are expensive.
The O2 indicator tablets are much cheaper, you will need to leave a vial in the container. I don't really know if the tablets would work well as a general indicator.
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Ferather]
#24764413 - 11/06/17 04:41 PM (6 years, 2 months ago) |
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Thank's you've just given me ideas on what to search for.
I will see if there is a chemical or biological way of determening the o2 and co2 levels inside the chamber.
I'm pretty sure I could find some alternative way of measuring this roughly after some research. What fruiting chambers do you use?
-------------------- Cakes inside Water Tub
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Mateja]
#24764939 - 11/06/17 08:12 PM (6 years, 2 months ago) |
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You're going to bring the wrath of the Mushroom Gods upon thee  The fuckers can be nasty if you don't keep it simple, or can give you a good spanking for dreaming up stuff that is not really cult related. You're better off using that inquisitiveness to find ways of making the hobby easier, make it yours, own it. You're going to forget that experiment idea as soon as you grow two successful tubs imo.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Re: Cultivation General Discussion [Re: Josex]
#24765403 - 11/07/17 02:37 AM (6 years, 2 months ago) |
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I've been having a real hard time cleaning up a clone, 4th transfer and it's still bacterial. Should I do a plate of antibacterial agar, or next transfer sandwich the myc with a piece of agar to allow it to grow up through it and hopefully leave the bacteria behind?
Since I pour my agar, how would I go about making 1 or 2 plates of antibacterial agar? I don't want to pour a whole sleeve of it, I want to avoid using antibacterial agar as much as possible.
I guess another option would be to scrap it and start fresh, it's not like it was some impressively large fruit or anything with a cool phenotype.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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