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OfflineFerather
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Registered: 03/19/15
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Re: Cultivation General Discussion [Re: Ferather]
    #24756932 - 11/03/17 12:34 PM (6 years, 2 months ago)

Dry spores test, soluble carbon and soluble macro-micro nutrients, controlled media.

----

Here I am testing another method of germinating dry spores from scratch, I'm using P. Cubensis var. Burma.
Recipe: 85g Water, 25g Paper Pellets, 2g MG, 0.25g YN. 1 x Cooked Wooden Peg, Total : £0.25.

The pellets contain soluble nutrients and complex cellulose, the peg contains the sugar.

   

----

Making the peg:

I sterilized the peg in 50g water + 4g sucrose, to make a sugar peg (use tweezers).
Remove the peg, flame it to remove excess water, or use tissue and alcohol.

Using a dry spore print, scratch and collect some spores on the tip.


Making the sub:

Tip: Use boiling hot water, as it breaks up the paper pellets almost instantly.

Add the MG and YN to a jug, add the water, mix, add to the pellets.
Mix and mash for 5 minutes, microwave for 48 seconds.

Mix, and assemble, microwave for 48 seconds.
Add the lid, and allow cooling for 2 hours.

----

Infections should be limited to-around the sugar peg.

Hopefully growth will be vigorous from the start


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineKyshroomer19xx
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Registered: 04/22/17
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24756934 - 11/03/17 12:35 PM (6 years, 2 months ago)

So just wipe it an rip it open pull off a piece and put it to agar and should I use tweezers to pull the piece off?


--------------------
RIP tom petty

Of course it is happening inside your head, but why on earth should that mean it is not real?
Albus  Dumbledore


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OfflineFerather
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Re: Cultivation General Discussion [Re: mushboy]
    #24756954 - 11/03/17 12:48 PM (6 years, 2 months ago)

Quote:

mushboy said:
Quote:

natedawgnow said:
@ mushboy- Instead of squirting sterile water over the dry spores, just make your agar media with a little less agar.
Makes it a little watery so that the spores have an easier time germinating if they are from an old print or something.




thats what i did with this one. but the growth was still shit.





Hmmm, I wonder if hydration under extreme nutrient level causes stronger genetics to overpower weaker genetics.
Or perhaps the stronger genetics recycles the weaker ones (disassemble) in order to survive when active.

If you hydrate spores on a fully supporting media, my guess would be the weak genetics merge?


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: Cultivation General Discussion [Re: Kyshroomer19xx]
    #24756956 - 11/03/17 12:49 PM (6 years, 2 months ago)

Flamed tweezers or flame a scalpel and cut out a piece, after you rip it open. Make sure you cool it in the agar. The reason we don't cut into it is it can push contamination into the inside as well as contaminate the flamed tool.


--------------------
JOIN THE POW WOW


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OfflineKyshroomer19xx
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Registered: 04/22/17
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24756961 - 11/03/17 12:51 PM (6 years, 2 months ago)

Ok cool thanks


--------------------
RIP tom petty

Of course it is happening inside your head, but why on earth should that mean it is not real?
Albus  Dumbledore


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Ferather]
    #24756972 - 11/03/17 12:59 PM (6 years, 2 months ago)

Quote:

Ferather said:
If you hydrate spores on a fully supporting media, my guess would be the weak genetics merge?




whatever the case, ive been getting the same shit results from a few different cubes. and a pan. 

im sure just dropping raw spores onto media isnt efficient either. i should be streaking it somehow to get more whateverhtefancywordis(diverse?) growth


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Offlinesir_spins_alot
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Registered: 04/04/10
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Re: Cultivation General Discussion [Re: Kyshroomer19xx] * 1
    #24756975 - 11/03/17 12:59 PM (6 years, 2 months ago)

I have a bunch of penis envy 6 that are colonizing full grain jars really fast from a ms syringe.  From there I've been using full colonized jars to do grain to grain and I have a shit ton of jars now.

  I ordered a bunch of bricks of coir and some Jiffy mix and a bunch of gypsum.  But since I had some jars done early before my coir got here I decided to just case some of my colonized wbs jars in a sgfc with straight verm.  I've done this year's ago with great success getting about 10-14 dry grams off a full quart of spawn.

  Humidity in the FC is reading high eighties and this is what the pins look like they're growing really slow my temps are 69f to 74f.  I've been misting them lightly with a continuous mist flairasol  mister in the morning then fan. Then I try to lightly fan at lunch,evening and right before lights out. 

Here's what they look like.  They've been pinning for about 3 days now with slow strange growth.





  The bottom pic with the weird shroom that looks like it has a stripe them a cap half way up the stripe them more stripe lol is the first pin i saw.  Very odd looking.


Edited by sir_spins_alot (11/03/17 01:05 PM)


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OfflineFerather
Mycological
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Registered: 03/19/15
Posts: 6,325
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Re: Cultivation General Discussion [Re: mushboy]
    #24757000 - 11/03/17 01:11 PM (6 years, 2 months ago)

Quote:

mushboy said:
Quote:

Ferather said:
If you hydrate spores on a fully supporting media, my guess would be the weak genetics merge?




whatever the case, ive been getting the same shit results from a few different cubes. and a pan. 

im sure just dropping raw spores onto media isnt efficient either. i should be streaking it somehow to get more whateverhtefancywordis(diverse?) growth




:takingnotes:


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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InvisibleSnazz
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Registered: 11/24/15
Posts: 1,584
Loc: Canada Flag
Re: Cultivation General Discussion [Re: Ferather]
    #24757021 - 11/03/17 01:25 PM (6 years, 2 months ago)

Quote:

Ferather said:
Quote:

mushboy said:
Quote:

natedawgnow said:
@ mushboy- Instead of squirting sterile water over the dry spores, just make your agar media with a little less agar.
Makes it a little watery so that the spores have an easier time germinating if they are from an old print or something.




thats what i did with this one. but the growth was still shit.





Hmmm, I wonder if hydration under extreme nutrient level causes stronger genetics to overpower weaker genetics.
Or perhaps the stronger genetics recycles the weaker ones (disassemble) in order to survive when active.

If you hydrate spores on a fully supporting media, my guess would be the weak genetics merge?




More likely it just triggers some genes to flip on or off (GRN) like in all life via environmental stimuli


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OfflineFerather
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Registered: 03/19/15
Posts: 6,325
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Re: Cultivation General Discussion [Re: Snazz] * 1
    #24757033 - 11/03/17 01:29 PM (6 years, 2 months ago)

That's a much better explanation, thanks.

:takingnotes:


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflineFerather
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Registered: 03/19/15
Posts: 6,325
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Re: Cultivation General Discussion [Re: Ferather]
    #24757052 - 11/03/17 01:38 PM (6 years, 2 months ago)

Some of my data:

With live mycelium, such as few grain samples, using slightly modified end media produced nice growth.
For cubensis, coir + malt extract + flour, 1g of each per 25g dry coir, add the lot to your water.

You will need a bigger container than a petri dish, isolate and transfer to grains.
The isolated growth should be ready for both the spawn and the coir.

I do similar but with lignicolous mycelium and add lignin.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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Offlinebaconcheese
Stranger

Registered: 10/11/17
Posts: 16
Last seen: 1 year, 2 months
Re: Cultivation General Discussion [Re: Ferather] * 1
    #24758175 - 11/04/17 12:06 AM (6 years, 2 months ago)

Beginning my journey to test for isolate vitality. Here are some tiny dicks...



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OfflineManifoldPrime
Per Ardua Ad Astra
I'm a teapot


Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 1 month
Re: Cultivation General Discussion [Re: baconcheese]
    #24758381 - 11/04/17 04:44 AM (6 years, 2 months ago)

Nice! Actual Microscopy! The spore cells look so complicated inside . . .


--------------------
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Please correct me if I say something wrong!

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Offline00Burnout
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Re: Cultivation General Discussion [Re: ManifoldPrime]
    #24758514 - 11/04/17 07:03 AM (6 years, 2 months ago)

Anyone else playing around with purple mystics? I spawned some yesterday in a mini, have some pf jars nearly finished colonizing and some plates going. I'm just curious to see if anyone else is getting really aggressive tomentose growth, there's some rhizo in my jars.

I always assumed tomentose was slow as hell but this shit is colonizing fast as fuck compared to my gt.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offlineicetech
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Registered: 08/21/17
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24758575 - 11/04/17 07:52 AM (6 years, 2 months ago)

Hadn't heard of them til recently, they look cool though.. when i get a print i will be playing with them for sure :smile:


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OfflineSteevo
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Re: Cultivation General Discussion [Re: icetech]
    #24758580 - 11/04/17 07:53 AM (6 years, 2 months ago)

The Black Cat is


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: Ferather]
    #24758581 - 11/04/17 07:54 AM (6 years, 2 months ago)

Quote:

Ferather said:
Quote:

mushboy said:
Quote:

Ferather said:
If you hydrate spores on a fully supporting media, my guess would be the weak genetics merge?




whatever the case, ive been getting the same shit results from a few different cubes. and a pan. 

im sure just dropping raw spores onto media isnt efficient either. i should be streaking it somehow to get more whateverhtefancywordis(diverse?) growth



:takingnotes:



Yea, streaking gives you many many more germination points, thus more myclial options to chose from in one plate.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: Cultivation General Discussion [Re: 00Burnout]
    #24758620 - 11/04/17 08:18 AM (6 years, 2 months ago)

Quote:

00Burnout said:
Anyone else playing around with purple mystics? I spawned some yesterday in a mini, have some pf jars nearly finished colonizing and some plates going. I'm just curious to see if anyone else is getting really aggressive tomentose growth, there's some rhizo in my jars.

I always assumed tomentose was slow as hell but this shit is colonizing fast as fuck compared to my gt.





I saw some amazing growth and got some big ass shrooms. This was from me fucking around with spore straight to grain for fun. Was bacterial as hell but went 5 flushes.



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Offline00Burnout
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24758679 - 11/04/17 08:54 AM (6 years, 2 months ago)

Damn I hope I get some monsters like that. What sub did you use in that mini and what ratio?


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Edited by 00Burnout (11/04/17 08:55 AM)


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InvisibleGerms
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Re: Cultivation General Discussion [Re: baconcheese]
    #24758728 - 11/04/17 09:19 AM (6 years, 2 months ago)

Quote:

baconcheese said:
Beginning my journey to test for isolate vitality. Here are some tiny dicks...





I really want a scope


--------------------


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