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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Ferather]
#24756932 - 11/03/17 12:34 PM (6 years, 2 months ago) |
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Dry spores test, soluble carbon and soluble macro-micro nutrients, controlled media.
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Here I am testing another method of germinating dry spores from scratch, I'm using P. Cubensis var. Burma. Recipe: 85g Water, 25g Paper Pellets, 2g MG, 0.25g YN. 1 x Cooked Wooden Peg, Total : £0.25. The pellets contain soluble nutrients and complex cellulose, the peg contains the sugar.
 ---- Making the peg: I sterilized the peg in 50g water + 4g sucrose, to make a sugar peg (use tweezers). Remove the peg, flame it to remove excess water, or use tissue and alcohol. Using a dry spore print, scratch and collect some spores on the tip. Making the sub: Tip: Use boiling hot water, as it breaks up the paper pellets almost instantly. Add the MG and YN to a jug, add the water, mix, add to the pellets. Mix and mash for 5 minutes, microwave for 48 seconds. Mix, and assemble, microwave for 48 seconds. Add the lid, and allow cooling for 2 hours. ---- Infections should be limited to-around the sugar peg.
Hopefully growth will be vigorous from the start
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Kyshroomer19xx
Est 19xx soldier



Registered: 04/22/17
Posts: 1,308
Loc:
Last seen: 4 years, 6 months
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Re: Cultivation General Discussion [Re: 00Burnout]
#24756934 - 11/03/17 12:35 PM (6 years, 2 months ago) |
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So just wipe it an rip it open pull off a piece and put it to agar and should I use tweezers to pull the piece off?
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: mushboy]
#24756954 - 11/03/17 12:48 PM (6 years, 2 months ago) |
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Quote:
mushboy said:
Quote:
natedawgnow said: @ mushboy- Instead of squirting sterile water over the dry spores, just make your agar media with a little less agar. Makes it a little watery so that the spores have an easier time germinating if they are from an old print or something.
thats what i did with this one. but the growth was still shit.

Hmmm, I wonder if hydration under extreme nutrient level causes stronger genetics to overpower weaker genetics. Or perhaps the stronger genetics recycles the weaker ones (disassemble) in order to survive when active.
If you hydrate spores on a fully supporting media, my guess would be the weak genetics merge?
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Flamed tweezers or flame a scalpel and cut out a piece, after you rip it open. Make sure you cool it in the agar. The reason we don't cut into it is it can push contamination into the inside as well as contaminate the flamed tool.
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Kyshroomer19xx
Est 19xx soldier



Registered: 04/22/17
Posts: 1,308
Loc:
Last seen: 4 years, 6 months
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Ok cool thanks
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Ferather]
#24756972 - 11/03/17 12:59 PM (6 years, 2 months ago) |
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Quote:
Ferather said: If you hydrate spores on a fully supporting media, my guess would be the weak genetics merge?
whatever the case, ive been getting the same shit results from a few different cubes. and a pan.
im sure just dropping raw spores onto media isnt efficient either. i should be streaking it somehow to get more whateverhtefancywordis(diverse?) growth
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sir_spins_alot
Feels like a stranger



Registered: 04/04/10
Posts: 640
Loc: United States
Last seen: 6 months, 26 days
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I have a bunch of penis envy 6 that are colonizing full grain jars really fast from a ms syringe. From there I've been using full colonized jars to do grain to grain and I have a shit ton of jars now.
I ordered a bunch of bricks of coir and some Jiffy mix and a bunch of gypsum. But since I had some jars done early before my coir got here I decided to just case some of my colonized wbs jars in a sgfc with straight verm. I've done this year's ago with great success getting about 10-14 dry grams off a full quart of spawn.
Humidity in the FC is reading high eighties and this is what the pins look like they're growing really slow my temps are 69f to 74f. I've been misting them lightly with a continuous mist flairasol mister in the morning then fan. Then I try to lightly fan at lunch,evening and right before lights out.
Here's what they look like. They've been pinning for about 3 days now with slow strange growth.


The bottom pic with the weird shroom that looks like it has a stripe them a cap half way up the stripe them more stripe lol is the first pin i saw. Very odd looking.
Edited by sir_spins_alot (11/03/17 01:05 PM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: mushboy]
#24757000 - 11/03/17 01:11 PM (6 years, 2 months ago) |
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Quote:
mushboy said:
Quote:
Ferather said: If you hydrate spores on a fully supporting media, my guess would be the weak genetics merge?
whatever the case, ive been getting the same shit results from a few different cubes. and a pan.
im sure just dropping raw spores onto media isnt efficient either. i should be streaking it somehow to get more whateverhtefancywordis(diverse?) growth
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,584
Loc: Canada
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Re: Cultivation General Discussion [Re: Ferather]
#24757021 - 11/03/17 01:25 PM (6 years, 2 months ago) |
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Quote:
Ferather said:
Quote:
mushboy said:
Quote:
natedawgnow said: @ mushboy- Instead of squirting sterile water over the dry spores, just make your agar media with a little less agar. Makes it a little watery so that the spores have an easier time germinating if they are from an old print or something.
thats what i did with this one. but the growth was still shit.

Hmmm, I wonder if hydration under extreme nutrient level causes stronger genetics to overpower weaker genetics. Or perhaps the stronger genetics recycles the weaker ones (disassemble) in order to survive when active.
If you hydrate spores on a fully supporting media, my guess would be the weak genetics merge?
More likely it just triggers some genes to flip on or off (GRN) like in all life via environmental stimuli
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Snazz] 1
#24757033 - 11/03/17 01:29 PM (6 years, 2 months ago) |
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That's a much better explanation, thanks.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Ferather]
#24757052 - 11/03/17 01:38 PM (6 years, 2 months ago) |
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Some of my data:
With live mycelium, such as few grain samples, using slightly modified end media produced nice growth. For cubensis, coir + malt extract + flour, 1g of each per 25g dry coir, add the lot to your water.
You will need a bigger container than a petri dish, isolate and transfer to grains. The isolated growth should be ready for both the spawn and the coir.
I do similar but with lignicolous mycelium and add lignin.
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baconcheese
Stranger

Registered: 10/11/17
Posts: 16
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Ferather] 1
#24758175 - 11/04/17 12:06 AM (6 years, 2 months ago) |
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Beginning my journey to test for isolate vitality. Here are some tiny dicks...
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 1 month
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Re: Cultivation General Discussion [Re: baconcheese]
#24758381 - 11/04/17 04:44 AM (6 years, 2 months ago) |
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Nice! Actual Microscopy! The spore cells look so complicated inside . . .
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Anyone else playing around with purple mystics? I spawned some yesterday in a mini, have some pf jars nearly finished colonizing and some plates going. I'm just curious to see if anyone else is getting really aggressive tomentose growth, there's some rhizo in my jars.
I always assumed tomentose was slow as hell but this shit is colonizing fast as fuck compared to my gt.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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icetech



Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
Last seen: 3 months, 5 days
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Re: Cultivation General Discussion [Re: 00Burnout]
#24758575 - 11/04/17 07:52 AM (6 years, 2 months ago) |
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Hadn't heard of them til recently, they look cool though.. when i get a print i will be playing with them for sure
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Steevo
Just a cog in the death wheel



Registered: 03/02/17
Posts: 2,702
Loc: Here and There
Last seen: 1 year, 9 months
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Re: Cultivation General Discussion [Re: icetech]
#24758580 - 11/04/17 07:53 AM (6 years, 2 months ago) |
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The Black Cat is
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Ferather]
#24758581 - 11/04/17 07:54 AM (6 years, 2 months ago) |
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Quote:
Ferather said:
Quote:
mushboy said:
Quote:
Ferather said: If you hydrate spores on a fully supporting media, my guess would be the weak genetics merge?
whatever the case, ive been getting the same shit results from a few different cubes. and a pan.
im sure just dropping raw spores onto media isnt efficient either. i should be streaking it somehow to get more whateverhtefancywordis(diverse?) growth

Yea, streaking gives you many many more germination points, thus more myclial options to chose from in one plate.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: 00Burnout]
#24758620 - 11/04/17 08:18 AM (6 years, 2 months ago) |
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Quote:
00Burnout said: Anyone else playing around with purple mystics? I spawned some yesterday in a mini, have some pf jars nearly finished colonizing and some plates going. I'm just curious to see if anyone else is getting really aggressive tomentose growth, there's some rhizo in my jars.
I always assumed tomentose was slow as hell but this shit is colonizing fast as fuck compared to my gt.
I saw some amazing growth and got some big ass shrooms. This was from me fucking around with spore straight to grain for fun. Was bacterial as hell but went 5 flushes.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Damn I hope I get some monsters like that. What sub did you use in that mini and what ratio?
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
Edited by 00Burnout (11/04/17 08:55 AM)
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: baconcheese]
#24758728 - 11/04/17 09:19 AM (6 years, 2 months ago) |
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Quote:
baconcheese said: Beginning my journey to test for isolate vitality. Here are some tiny dicks...

I really want a scope
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