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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: mushboy]
#24756770 - 11/03/17 11:35 AM (6 years, 2 months ago) |
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@mushboy
I've started doing some work with dry spores, I'll post my experiment soon. At a guess, id say its the way solubles travel in water.
I guess a hydrated spores have better travel. Cant be soluble without water.
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It also possible the absorbed fluids contain trace nutrients. That may be enough to produce a "seed" spore.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Quote:
Kyshroomer19xx said: When cloning where is the best area to take a cut from this is my first attempt at cloning
Inner tissue at base of stipe. Wipe with iso and rip open, don't cut into it or you'll force contams into the shroom and onto your blade. I made that mistake and I'm still cleaning it up...I might have to make some tea agar to help it along
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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Explicitdetail
Rickshaw Bandit



Registered: 02/03/17
Posts: 592
Loc: North of the Equator
Last seen: 1 month, 8 days
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Re: Cultivation General Discussion [Re: 00Burnout]
#24756774 - 11/03/17 11:37 AM (6 years, 2 months ago) |
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You wipe the mushroom down with ISO? Seems unnecessary if your taking an inner tissue sample
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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Drayce
Spidget Finner



Registered: 10/07/17
Posts: 260
Loc: Gaia
Last seen: 2 months, 11 days
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Re: Cultivation General Discussion [Re: Ferather]
#24756776 - 11/03/17 11:38 AM (6 years, 2 months ago) |
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Quote:
Ferather said: 40kg of soft building sand is £2.40. Gritty compost is very good for some substrates.
You could try compost and grain, but compost contains various nutrients. I've never tried it, but it may increase the contamination risk.
Sulfur is used for certain amino acids and unit binding. Calcium is used for oxalate's and unit binding.
Use depends on the recipe and media.
It almost appears as you use a straightedge to line up your sentences.
But, it could just be my vivid and wandering imagination.
That causes me to to see things there.
That might not be.
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                 “The moment is where we spend most of our time.” – Terence McKenna
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: 00Burnout]
#24756778 - 11/03/17 11:38 AM (6 years, 2 months ago) |
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@ mushboy- Instead of squirting sterile water over the dry spores, just make your agar media with a little less agar. Makes it a little watery so that the spores have an easier time germinating if they are from an old print or something.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Drayce]
#24756783 - 11/03/17 11:39 AM (6 years, 2 months ago) |
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Quote:
Drayce said:
Quote:
Ferather said: 40kg of soft building sand is £2.40. Gritty compost is very good for some substrates.
You could try compost and grain, but compost contains various nutrients. I've never tried it, but it may increase the contamination risk.
Sulfur is used for certain amino acids and unit binding. Calcium is used for oxalate's and unit binding.
Use depends on the recipe and media.
It almost appears as you use a straightedge to line up your sentences.
But, it could just be my vivid and wandering imagination.
That causes me to to see things there.
That might not be.
I have ADHD with traits of OCD. That's normal for ADHD!
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: Ferather]
#24756791 - 11/03/17 11:42 AM (6 years, 2 months ago) |
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I have never worked with hydrated spores until last night. I wasn’t sure how to get the spores in the water so I took a sterile syringe, attached a needle, flamed it, sucked up some sterile water and squirted the water on a portion of the print and let it run off into an ISO sanitized glass bowl that I flamed with the torch before placing in the SAB. Then I sucked the water back into the syringe.
It was on a whim I decided to create my own spore syringe, came out to like 14cc I split across 7 qts
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: natedawgnow]
#24756796 - 11/03/17 11:44 AM (6 years, 2 months ago) |
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Quote:
natedawgnow said: @ mushboy- Instead of squirting sterile water over the dry spores, just make your agar media with a little less agar. Makes it a little watery so that the spores have an easier time germinating if they are from an old print or something.
thats what i did with this one. but the growth was still shit.
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Drayce
Spidget Finner



Registered: 10/07/17
Posts: 260
Loc: Gaia
Last seen: 2 months, 11 days
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Re: Cultivation General Discussion [Re: Ferather]
#24756798 - 11/03/17 11:45 AM (6 years, 2 months ago) |
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Yeah Ferather- I noticed that bit.
with the first few data dumps I looked at
I use gypsum because it seemed to be the thing to do.
I don't see any disadvantages over using it or not,
and it is not expensive around here.
Lots of home brewing
Places around
here.
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                 “The moment is where we spend most of our time.” – Terence McKenna
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: natedawgnow]
#24756803 - 11/03/17 11:46 AM (6 years, 2 months ago) |
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Can anyone recommend an agar recipe that will encourage fast growth? I tried 10 grams: 10 grams: drop of karo per 500 ml. Very slow growth.
I have agar powder, potato flakes and karo.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: JHOVA]
#24756814 - 11/03/17 11:49 AM (6 years, 2 months ago) |
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get brf. but pda is really good too.
6g brf and 9grams agar powder. its really good and easy to obtain. Light malt is my fav tho. shits clear like glass
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: mushboy]
#24756824 - 11/03/17 11:52 AM (6 years, 2 months ago) |
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6 grams potato flakes: 9 grams agar-agar powder: how much karo if at all?
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: mushboy]
#24756827 - 11/03/17 11:53 AM (6 years, 2 months ago) |
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LME is my go to as well. Comes out clear with no need to filter. I use 5g malt to 10g agar to 450ml water. Most cultures consume a plate in 10-14 days
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: JHOVA]
#24756835 - 11/03/17 11:55 AM (6 years, 2 months ago) |
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Quote:
Jonhova said: 6 grams potato flakes: 9 grams agar-agar powder: how much karo if at all?
wut? you asked for agar besides pda. so i gave you a brf recipe. no shitty karo with brf agar.
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: mushboy]
#24756843 - 11/03/17 11:57 AM (6 years, 2 months ago) |
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Should have clarified i meant ratio, not ingredients.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: mushboy]
#24756846 - 11/03/17 11:59 AM (6 years, 2 months ago) |
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What I did with my last go around of dry spores was I took a bunch of Q tips, moistened them, then put in a jar and sterilized them. Then in the lab used the moist swab to grab some spores and swiped the plate. It has worked rather well for the wood lovers I was having issues with.
-------------------- JOIN THE POW WOW
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: JHOVA]
#24756856 - 11/03/17 12:01 PM (6 years, 2 months ago) |
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cut back on the pda? i think nute heavy agar tends to keep growth kinda clustered up whereas lighter nutes make it 'reach' out.
or something.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: mushboy]
#24756867 - 11/03/17 12:03 PM (6 years, 2 months ago) |
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-------------------- JOIN THE POW WOW
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icetech



Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
Last seen: 3 months, 5 days
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Re: Cultivation General Discussion [Re: mushboy]
#24756904 - 11/03/17 12:18 PM (6 years, 2 months ago) |
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I only used the potato agar once.. switched to BRF agar and love it.. myc seems to also, but my potato was always really cloudy looking the bfr much clearer and more uniform
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Drayce]
#24756919 - 11/03/17 12:29 PM (6 years, 2 months ago) |
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Quote:
Drayce said: Yeah Ferather- I noticed that bit.
with the first few data dumps I looked at
I use gypsum because it seemed to be the thing to do.
I don't see any disadvantages over using it or not,
and it is not expensive around here.
Lots of home brewing
Places around
here.
Cool. If it's cheap and doing what you want, I cant say I see an issue. Just don't use calcium carbonate (CaCO3), as mentioned.
Grain pH is pH 5.5-6.8. Coir is also pH 5.5-6.8.
pH 5.5 vs 6.8 could be interesting.
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