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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24747550 - 10/30/17 12:05 PM (6 years, 2 months ago)

ill toss a germ plate into a spare jar of grains just for shits but you never inoculate with a germ plate as you main grow.

ive done it before and it was out of laziness. bad practices leads to bad habits and bad results.


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InvisibleTheMadHatter420
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Re: Cultivation General Discussion [Re: mushboy]
    #24747558 - 10/30/17 12:09 PM (6 years, 2 months ago)

I usually let them sit to see if I get some pins, but usually end up needing the plate before it reaches that point.


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Invisiblevan hattonFacebookDiscord
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24747561 - 10/30/17 12:11 PM (6 years, 2 months ago)

I usually do 2 transfers before inoculation. Depending on how it looks


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24747571 - 10/30/17 12:16 PM (6 years, 2 months ago)

Quote:

TheMadHatter420 said:
Ok you will never use a germination plate to inoculate grains. A germination plate will never be 100% clean. It takes several transfers to get away from contamination.



Good advice.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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OfflineExplicitdetail
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Re: Cultivation General Discussion [Re: hamloaf]
    #24747579 - 10/30/17 12:21 PM (6 years, 2 months ago)

So this isn’t exactly a germ plate but I used a germ plate in hopes of getting a nice pin to clone, this is the pin  transferred to a new plate and grown out. This plate was put to grains with great success and zero contams


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"Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: Explicitdetail]
    #24747581 - 10/30/17 12:22 PM (6 years, 2 months ago)

:borat:


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How I Get Stuff done. - My Reference Guide. - My Grows.


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OfflineExplicitdetail
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Re: Cultivation General Discussion [Re: hamloaf]
    #24747588 - 10/30/17 12:24 PM (6 years, 2 months ago)

More or less saying if the germ plate is clean I don’t see a reason not to use it


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"Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Explicitdetail]
    #24747601 - 10/30/17 12:27 PM (6 years, 2 months ago)

you didnt use a germ plate. bad comparison:shrug: you snagged a pin, which would grow inside a sterile environment.(the plates)

but next time id take cuts from that pin plate and put the pin plate on ice. instead of tossing the whole thing in grains.


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: mushboy]
    #24747607 - 10/30/17 12:29 PM (6 years, 2 months ago)

A germ plate and a cloned culture plate are like comparing apples to oranges.  Either way, when working with a cloned culture from pin, unless said pin came from a sealed agar plate, then at least one security is in order.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleTheMadHatter420
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Re: Cultivation General Discussion [Re: mushboy]
    #24747616 - 10/30/17 12:31 PM (6 years, 2 months ago)

EDIT
Sorry Ham that was at Explicit


Germ plates will not be clean though. Mother nature will have bacteria with the spores. you may not see it, but the bacteria spores will be there. You may be able to get away with one, every now and the, as an experienced grower, but this is BAD advice to give to a noob. One must first learn the rules, before they can attempt to break them. No reason to start new people down a risky road.


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Edited by TheMadHatter420 (10/30/17 12:42 PM)


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24747620 - 10/30/17 12:32 PM (6 years, 2 months ago)

Who are you talking to?


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How I Get Stuff done. - My Reference Guide. - My Grows.


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OfflineExplicitdetail
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Re: Cultivation General Discussion [Re: hamloaf]
    #24747625 - 10/30/17 12:38 PM (6 years, 2 months ago)

I just meant the pin was grown on a germ plate, transferred, and proved to be 100% clean. If the original plate was dirty I feel like after 2 months the pin would have picked up something if it was there.


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"Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."


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InvisibleGerms
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Re: Cultivation General Discussion [Re: Explicitdetail] * 1
    #24747626 - 10/30/17 12:39 PM (6 years, 2 months ago)

Did someone call me?


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Invisiblevan hattonFacebookDiscord
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Re: Cultivation General Discussion [Re: Germs]
    #24747628 - 10/30/17 12:40 PM (6 years, 2 months ago)

:lol:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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OfflineExplicitdetail
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Re: Cultivation General Discussion [Re: mushboy]
    #24747642 - 10/30/17 12:44 PM (6 years, 2 months ago)

Quote:

mushboy said:
but next time id take cuts from that pin plate and put the pin plate on ice. instead of tossing the whole thing in grains.



Still have this plate and the orignal plate in storage


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"Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."


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OfflineKyshroomer19xx
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24747832 - 10/30/17 02:26 PM (6 years, 2 months ago)

Quote:

TheMadHatter420 said:
Ok you will never use a germination plate to inoculate grains. A germination plate will never be 100% clean. It takes several transfers to get away from contamination.




Yea I know that I would need to do multiple transfers before inoculating I was just wanting to know what you guys thought of the plate. I am going to give it awhile and see what happens


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InvisibleTheMadHatter420
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Re: Cultivation General Discussion [Re: Kyshroomer19xx]
    #24747864 - 10/30/17 02:46 PM (6 years, 2 months ago)

Take transfers now. Waiting allows contamination a chance to fuck shit up. The goal, when working with agar, is to take transfers, from the cleanest area, as soon as possible. Your trying to grab mycelium that is out running the contamination. IF you let the mycelium hit the glass the contamination an catch up. It is always good to take the transfer from germination plate ASAP. I try to take mine as soon as I am sure it is mycelium and not mold. The p. serbica I just transferred were done when the mycelium was about half the size of a pencil eraser.


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OfflineKyshroomer19xx
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24747965 - 10/30/17 03:44 PM (6 years, 2 months ago)



So is this for sure myc  and not mold?


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RIP tom petty

Of course it is happening inside your head, but why on earth should that mean it is not real?
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Offline00Burnout
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Re: Cultivation General Discussion [Re: Kyshroomer19xx]
    #24748156 - 10/30/17 05:09 PM (6 years, 2 months ago)

I can't tell from the pic, but since pasty plates can be reused you're better off transferring anyway. How long since you inoculated that plate? IME if it's mold the sporophore will develop around 2-3 days after noticeable growth. All the mold I've had grow was extremely uniform. MS is never uniform, clones are rarely uniform (only in the lucky case that it's an isolate to begin with), and of course monocultures are uniform. At least this is what I've gathered from all the reading I've done on agar work. Someone with more experience please correct me if I'm wrong.


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OfflineVariegated
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24748336 - 10/30/17 06:45 PM (6 years, 2 months ago)

All my truffle transfers grow out really even and organized and i doubt there a mono but thats the only one i have like that.


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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   North Spore Injection Grain Bag   PhytoExtractum Buy Bali Kratom Powder


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