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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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ill toss a germ plate into a spare jar of grains just for shits but you never inoculate with a germ plate as you main grow.
ive done it before and it was out of laziness. bad practices leads to bad habits and bad results.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: mushboy]
#24747558 - 10/30/17 12:09 PM (6 years, 2 months ago) |
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I usually let them sit to see if I get some pins, but usually end up needing the plate before it reaches that point.
-------------------- JOIN THE POW WOW
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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I usually do 2 transfers before inoculation. Depending on how it looks
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Quote:
TheMadHatter420 said: Ok you will never use a germination plate to inoculate grains. A germination plate will never be 100% clean. It takes several transfers to get away from contamination.
Good advice.
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Explicitdetail
Rickshaw Bandit



Registered: 02/03/17
Posts: 592
Loc: North of the Equator
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Re: Cultivation General Discussion [Re: hamloaf]
#24747579 - 10/30/17 12:21 PM (6 years, 2 months ago) |
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So this isn’t exactly a germ plate but I used a germ plate in hopes of getting a nice pin to clone, this is the pin transferred to a new plate and grown out. This plate was put to grains with great success and zero contams
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Explicitdetail
Rickshaw Bandit



Registered: 02/03/17
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Re: Cultivation General Discussion [Re: hamloaf]
#24747588 - 10/30/17 12:24 PM (6 years, 2 months ago) |
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More or less saying if the germ plate is clean I don’t see a reason not to use it
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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you didnt use a germ plate. bad comparison you snagged a pin, which would grow inside a sterile environment.(the plates)
but next time id take cuts from that pin plate and put the pin plate on ice. instead of tossing the whole thing in grains.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: mushboy]
#24747607 - 10/30/17 12:29 PM (6 years, 2 months ago) |
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A germ plate and a cloned culture plate are like comparing apples to oranges. Either way, when working with a cloned culture from pin, unless said pin came from a sealed agar plate, then at least one security is in order.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: mushboy]
#24747616 - 10/30/17 12:31 PM (6 years, 2 months ago) |
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EDIT Sorry Ham that was at Explicit
Germ plates will not be clean though. Mother nature will have bacteria with the spores. you may not see it, but the bacteria spores will be there. You may be able to get away with one, every now and the, as an experienced grower, but this is BAD advice to give to a noob. One must first learn the rules, before they can attempt to break them. No reason to start new people down a risky road.
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Edited by TheMadHatter420 (10/30/17 12:42 PM)
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Who are you talking to?
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Explicitdetail
Rickshaw Bandit



Registered: 02/03/17
Posts: 592
Loc: North of the Equator
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Re: Cultivation General Discussion [Re: hamloaf]
#24747625 - 10/30/17 12:38 PM (6 years, 2 months ago) |
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I just meant the pin was grown on a germ plate, transferred, and proved to be 100% clean. If the original plate was dirty I feel like after 2 months the pin would have picked up something if it was there.
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Did someone call me?
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Germs]
#24747628 - 10/30/17 12:40 PM (6 years, 2 months ago) |
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-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Explicitdetail
Rickshaw Bandit



Registered: 02/03/17
Posts: 592
Loc: North of the Equator
Last seen: 1 month, 8 days
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Re: Cultivation General Discussion [Re: mushboy]
#24747642 - 10/30/17 12:44 PM (6 years, 2 months ago) |
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Quote:
mushboy said: but next time id take cuts from that pin plate and put the pin plate on ice. instead of tossing the whole thing in grains.
Still have this plate and the orignal plate in storage
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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Kyshroomer19xx
Est 19xx soldier



Registered: 04/22/17
Posts: 1,308
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Last seen: 4 years, 6 months
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Quote:
TheMadHatter420 said: Ok you will never use a germination plate to inoculate grains. A germination plate will never be 100% clean. It takes several transfers to get away from contamination.
Yea I know that I would need to do multiple transfers before inoculating I was just wanting to know what you guys thought of the plate. I am going to give it awhile and see what happens
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Take transfers now. Waiting allows contamination a chance to fuck shit up. The goal, when working with agar, is to take transfers, from the cleanest area, as soon as possible. Your trying to grab mycelium that is out running the contamination. IF you let the mycelium hit the glass the contamination an catch up. It is always good to take the transfer from germination plate ASAP. I try to take mine as soon as I am sure it is mycelium and not mold. The p. serbica I just transferred were done when the mycelium was about half the size of a pencil eraser.
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Kyshroomer19xx
Est 19xx soldier



Registered: 04/22/17
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So is this for sure myc and not mold?
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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I can't tell from the pic, but since pasty plates can be reused you're better off transferring anyway. How long since you inoculated that plate? IME if it's mold the sporophore will develop around 2-3 days after noticeable growth. All the mold I've had grow was extremely uniform. MS is never uniform, clones are rarely uniform (only in the lucky case that it's an isolate to begin with), and of course monocultures are uniform. At least this is what I've gathered from all the reading I've done on agar work. Someone with more experience please correct me if I'm wrong.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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Variegated
Pressure cooker operator



Registered: 01/15/17
Posts: 820
Last seen: 2 years, 3 months
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Re: Cultivation General Discussion [Re: 00Burnout]
#24748336 - 10/30/17 06:45 PM (6 years, 2 months ago) |
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All my truffle transfers grow out really even and organized and i doubt there a mono but thats the only one i have like that.
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