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InvisibleInocuole
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Re: Cultivation General Discussion [Re: La Flama Blanca]
    #24739329 - 10/26/17 03:21 PM (6 years, 3 months ago)

Look how he put his polyfill.  Dude probably had it coming.


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OfflineLa Flama Blanca
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Re: Cultivation General Discussion [Re: Inocuole]
    #24739409 - 10/26/17 04:04 PM (6 years, 3 months ago)

lol i didnt notice that, but yea look at the one in the top left corner. #what


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InvisibleInocuole
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Re: Cultivation General Discussion [Re: La Flama Blanca]
    #24739415 - 10/26/17 04:06 PM (6 years, 3 months ago)

If he was that sloppy about stuffing poly he was probably pretty sloppy about his opsec, was my thought.  :shrug:


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineLa Flama Blanca
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Re: Cultivation General Discussion [Re: Inocuole]
    #24739419 - 10/26/17 04:07 PM (6 years, 3 months ago)

Good point. like they say, how you do anything....


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InvisibleMateja
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Re: Cultivation General Discussion [Re: Ferather]
    #24739936 - 10/26/17 08:27 PM (6 years, 3 months ago)

Quote:

Ferather said:
Adjusted recipe for you:

100ml boiling hot water, 2g dog food, 4g tea, 1-2g any sugar, 2.8g agar.




Quote:

Ferather said:
For cubensis, Mycolorado's recipe, no gelatin:

200g > Boiling hot water.
2g > Malt extract.
4.25g > Tea.
4g> Agar.





I'm a little confused. You first suggested a recipe with 4% tea and later you suggest another recipe with 2% Tea (half the strength) And why 2.8% agar? Why does the agar need to be stiffer than usual?


My next recipe will be:

200ml WBS soak water
4g Tea
4g Agar
(1-2g sugars?) as you suggested earlier? Or are sugars not necessary along with grain water agar?

Do you see a problem with this recipe or will this recipe allow mycelium to digest the nutrients? My last 20 petris, I kind of don't want to waste them :shrug: antibacterial agar is new to me and I would appreciate any input to clarify this for me.


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: Mateja] * 1
    #24740203 - 10/26/17 10:19 PM (6 years, 3 months ago)

The sugars aren't necessary.


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OfflineManifoldPrime
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Re: Cultivation General Discussion [Re: hamloaf]
    #24740487 - 10/27/17 03:34 AM (6 years, 3 months ago)

I wonder if anyone has seen this before:

I took a pin off some texan that was on agar for a long time , and took a transfer from the clone's plate once it colonised it. However, the pin itself became the site of more pins:





Bonus pic:



Its the weirdest thing. Im gonna put 2-3 of those pins on fresh plates.

I just got a batch of bigger 90ml petris . . .



Just waiting and smoking doinks until till I can pour. . .


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OfflineFerather
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Re: Cultivation General Discussion [Re: ManifoldPrime] * 1
    #24740605 - 10/27/17 06:28 AM (6 years, 3 months ago)

Sorry Mateah, I have confused you, I personally don't use sugar because of the infection issues.
Cubensis can eat just proteins + macro-micro nutrients from the tea solubles to grow.

Both myself and pacmanbreed got live cubensis growth on ~0.8g gelatin.
Gelatin doesn't germinate much-anything alone, without sugars.

The alternative option, is to make a normal agar recipe.
And the use the tea extract as an antibiotic.

Gelatin makes agar softer than normal.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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InvisibleGerms
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Re: Cultivation General Discussion [Re: Ferather]
    #24740828 - 10/27/17 09:07 AM (6 years, 3 months ago)


Can this be used for bulk sub?


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Invisiblevan hattonFacebookDiscord
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Re: Cultivation General Discussion [Re: Germs]
    #24740864 - 10/27/17 09:43 AM (6 years, 3 months ago)

Yeah just expensive. I get a bale for 5.50. just run it over with a lawn mower to get small pieces.


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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OfflineBigbadwooof
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Re: Cultivation General Discussion [Re: Germs]
    #24740874 - 10/27/17 09:48 AM (6 years, 3 months ago)

Quote:

Germs said:
Had to stay a little late at work today and it’s pretty slow, decided to search up mushroom busts in my state, found this gem from 2015



They only found 9 dry ounces in the place, makes you wonder where the rest went but in a hippie place like Austin it’s no surprise lol

the article said “officers were warned to evacuate the house because mature mushrooms are known to release spores that can br harmful when inhaled, if not fatal” and I’m not sure that i’ve ever read something so stupid in my entire life




What was the sentence?


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"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
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InvisibleGerms
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Re: Cultivation General Discussion [Re: Bigbadwooof]
    #24740880 - 10/27/17 09:52 AM (6 years, 3 months ago)

The article said no arrests were made at the time of being published.

Anyone got a source for straw pellets?


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Invisiblevan hattonFacebookDiscord
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Re: Cultivation General Discussion [Re: Germs]
    #24740885 - 10/27/17 09:56 AM (6 years, 3 months ago)

What's your growth rates look like 5 days after a transfer?

Mine seems sluggish maybe a half an inch grew since Sunday. It still has a transparent look.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Offline00Burnout
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Re: Cultivation General Discussion [Re: van hatton]
    #24741010 - 10/27/17 10:41 AM (6 years, 3 months ago)

Well I just started playing with agar so this is my first succesful culture. 5 days after transferring from my master I was ready to put to grain, or brf in this case.

5 days after putting to brf I couldn't see much, it was still crawling across the top. The thing that sucks is I can't shake the jars at all so I can't speed things up.

If this works out I'm going to rye or wbs depending on how much rye will run me, I'll probably just do a bunch of mini monos and keep doing cakes for testing lc, isolates and just because cakes are fun.


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Peace, pot and microdot!:mushroom2:
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InvisibleMateja
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Re: Cultivation General Discussion [Re: Mateja]
    #24741077 - 10/27/17 11:06 AM (6 years, 3 months ago)

Quote:

Ferather said:
Sorry Mateah, I have confused you. The alternative option, is to make a normal agar recipe.
And the use the tea extract as an antibiotic.




But I did just that, I used a normal agar recipe and added the tea as an antibiotic :shrug:
Quote:

Mateah said:
I ended up doing black tea agar. 200ml water, 2g dog food, 4g tea and 4g agar.




I normally  make DFA at 2% strength but 1% works for me to, the culture still grows fine on 1%, but the culture on the tea recipe never ever recovered.



And for a comparison with other plates with only Dog food and no tea

So if I didn't need added sugars, why is there no recovery from the tea agar plates?


Edited by Mateja (10/27/17 11:10 AM)


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Invisiblevan hattonFacebookDiscord
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24741130 - 10/27/17 11:35 AM (6 years, 3 months ago)

I actually picked up some brf a few days ago to play around.

Just picked up 50lbs of rye for 14 bucks.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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OfflineDrayce
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Re: Cultivation General Discussion [Re: Germs]
    #24741221 - 10/27/17 12:17 PM (6 years, 3 months ago)

Quote:

Germs said:

Can this be used for bulk sub?



Yes, I'm using that right now for Oyters and cubes.
I ordered it for pick-up, went to store, & outside they had pallets of  bigger 'clean' bales way cheaper....:foreheadslap:
But it is relatively clean and easy to work with IME.

Just used RR's 'lets do straw' method, I used pickling lime- works great.


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OfflineFerather
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Re: Cultivation General Discussion [Re: Mateja]
    #24741236 - 10/27/17 12:27 PM (6 years, 3 months ago)

@Mateah

Well I can see it decaying the dog food, there is goo around it.
The result is, it doesn't like the tea, or the mixture.

You could try gelatin, else I don't know.


Scenario:

The dog food may be enough for minimal support.
Then add inhibitory, and it's not enough.


The composition of the DF = unknown.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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InvisibleMateja
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Re: Cultivation General Discussion [Re: Ferather]
    #24741244 - 10/27/17 12:36 PM (6 years, 3 months ago)

Next recipe will be

200ml WBS soak water
4g Tea
4g Agar

Grain water has known nutrients and is very rich. DO you agree that this 'should' work?


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OfflineFerather
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Re: Cultivation General Discussion [Re: Mateja]
    #24741257 - 10/27/17 12:47 PM (6 years, 3 months ago)

Yes it has starch in it, which is made from sugars, it will be used to grow and overcome effects.
The down side, it increases the chance of an invader of doing the same, and growing.

Personally I think you may be better off using 1-2g of any grain flour.
Just so you can measure how much is too much, too little.

You should aim for the minimum needed for growth.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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