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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Look how he put his polyfill. Dude probably had it coming.
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La Flama Blanca
Stranger


Registered: 01/15/16
Posts: 487
Last seen: 10 months, 28 days
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Re: Cultivation General Discussion [Re: Inocuole]
#24739409 - 10/26/17 04:04 PM (6 years, 3 months ago) |
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lol i didnt notice that, but yea look at the one in the top left corner. #what
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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If he was that sloppy about stuffing poly he was probably pretty sloppy about his opsec, was my thought.
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La Flama Blanca
Stranger


Registered: 01/15/16
Posts: 487
Last seen: 10 months, 28 days
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Re: Cultivation General Discussion [Re: Inocuole]
#24739419 - 10/26/17 04:07 PM (6 years, 3 months ago) |
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Good point. like they say, how you do anything....
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Ferather]
#24739936 - 10/26/17 08:27 PM (6 years, 3 months ago) |
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Quote:
Ferather said: Adjusted recipe for you:
100ml boiling hot water, 2g dog food, 4g tea, 1-2g any sugar, 2.8g agar.
Quote:
Ferather said: For cubensis, Mycolorado's recipe, no gelatin:
200g > Boiling hot water. 2g > Malt extract. 4.25g > Tea. 4g> Agar.
I'm a little confused. You first suggested a recipe with 4% tea and later you suggest another recipe with 2% Tea (half the strength) And why 2.8% agar? Why does the agar need to be stiffer than usual?
My next recipe will be:
200ml WBS soak water 4g Tea 4g Agar (1-2g sugars?) as you suggested earlier? Or are sugars not necessary along with grain water agar?
Do you see a problem with this recipe or will this recipe allow mycelium to digest the nutrients? My last 20 petris, I kind of don't want to waste them antibacterial agar is new to me and I would appreciate any input to clarify this for me.
-------------------- Cakes inside Water Tub
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Mateja] 1
#24740203 - 10/26/17 10:19 PM (6 years, 3 months ago) |
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The sugars aren't necessary.
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 1 month
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Re: Cultivation General Discussion [Re: hamloaf]
#24740487 - 10/27/17 03:34 AM (6 years, 3 months ago) |
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I wonder if anyone has seen this before:
I took a pin off some texan that was on agar for a long time , and took a transfer from the clone's plate once it colonised it. However, the pin itself became the site of more pins:


Bonus pic:

Its the weirdest thing. Im gonna put 2-3 of those pins on fresh plates.
I just got a batch of bigger 90ml petris . . .

Just waiting and smoking doinks until till I can pour. . .
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: ManifoldPrime] 1
#24740605 - 10/27/17 06:28 AM (6 years, 3 months ago) |
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Sorry Mateah, I have confused you, I personally don't use sugar because of the infection issues. Cubensis can eat just proteins + macro-micro nutrients from the tea solubles to grow.
Both myself and pacmanbreed got live cubensis growth on ~0.8g gelatin. Gelatin doesn't germinate much-anything alone, without sugars.
The alternative option, is to make a normal agar recipe. And the use the tea extract as an antibiotic.
Gelatin makes agar softer than normal.
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: Ferather]
#24740828 - 10/27/17 09:07 AM (6 years, 3 months ago) |
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 Can this be used for bulk sub?
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Germs]
#24740864 - 10/27/17 09:43 AM (6 years, 3 months ago) |
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Yeah just expensive. I get a bale for 5.50. just run it over with a lawn mower to get small pieces.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Bigbadwooof
Trumps Bone Spurs



Registered: 12/07/13
Posts: 13,347
Last seen: 6 hours, 7 minutes
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Re: Cultivation General Discussion [Re: Germs]
#24740874 - 10/27/17 09:48 AM (6 years, 3 months ago) |
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Quote:
Germs said: Had to stay a little late at work today and it’s pretty slow, decided to search up mushroom busts in my state, found this gem from 2015

They only found 9 dry ounces in the place, makes you wonder where the rest went but in a hippie place like Austin it’s no surprise lol
the article said “officers were warned to evacuate the house because mature mushrooms are known to release spores that can br harmful when inhaled, if not fatal” and I’m not sure that i’ve ever read something so stupid in my entire life
What was the sentence?
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti FARTS "There is no need for conspiracy where interests converge" - George Carlin Every one of you should see this video. "If you bombard the earth with photons for a while, it can emit a roadster" - Andrej Kerpathy
 
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: Bigbadwooof]
#24740880 - 10/27/17 09:52 AM (6 years, 3 months ago) |
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The article said no arrests were made at the time of being published.
Anyone got a source for straw pellets?
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Germs]
#24740885 - 10/27/17 09:56 AM (6 years, 3 months ago) |
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What's your growth rates look like 5 days after a transfer?
Mine seems sluggish maybe a half an inch grew since Sunday. It still has a transparent look.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 6 months, 21 days
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Re: Cultivation General Discussion [Re: van hatton]
#24741010 - 10/27/17 10:41 AM (6 years, 3 months ago) |
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Well I just started playing with agar so this is my first succesful culture. 5 days after transferring from my master I was ready to put to grain, or brf in this case.
5 days after putting to brf I couldn't see much, it was still crawling across the top. The thing that sucks is I can't shake the jars at all so I can't speed things up.
If this works out I'm going to rye or wbs depending on how much rye will run me, I'll probably just do a bunch of mini monos and keep doing cakes for testing lc, isolates and just because cakes are fun.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Mateja]
#24741077 - 10/27/17 11:06 AM (6 years, 3 months ago) |
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Quote:
Ferather said: Sorry Mateah, I have confused you. The alternative option, is to make a normal agar recipe. And the use the tea extract as an antibiotic.
But I did just that, I used a normal agar recipe and added the tea as an antibiotic 
Quote:
Mateah said: I ended up doing black tea agar. 200ml water, 2g dog food, 4g tea and 4g agar.
I normally make DFA at 2% strength but 1% works for me to, the culture still grows fine on 1%, but the culture on the tea recipe never ever recovered.
 
And for a comparison with other plates with only Dog food and no tea
  So if I didn't need added sugars, why is there no recovery from the tea agar plates?
Edited by Mateja (10/27/17 11:10 AM)
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: 00Burnout]
#24741130 - 10/27/17 11:35 AM (6 years, 3 months ago) |
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I actually picked up some brf a few days ago to play around.
Just picked up 50lbs of rye for 14 bucks.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Drayce
Spidget Finner



Registered: 10/07/17
Posts: 260
Loc: Gaia
Last seen: 2 months, 11 days
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Re: Cultivation General Discussion [Re: Germs]
#24741221 - 10/27/17 12:17 PM (6 years, 3 months ago) |
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Quote:
Germs said:
 Can this be used for bulk sub?
Yes, I'm using that right now for Oyters and cubes. I ordered it for pick-up, went to store, & outside they had pallets of bigger 'clean' bales way cheaper.... But it is relatively clean and easy to work with IME.
Just used RR's 'lets do straw' method, I used pickling lime- works great.
--------------------
                 “The moment is where we spend most of our time.” – Terence McKenna
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Mateja]
#24741236 - 10/27/17 12:27 PM (6 years, 3 months ago) |
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@Mateah
Well I can see it decaying the dog food, there is goo around it. The result is, it doesn't like the tea, or the mixture.
You could try gelatin, else I don't know.
Scenario:
The dog food may be enough for minimal support. Then add inhibitory, and it's not enough.
The composition of the DF = unknown.
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Ferather]
#24741244 - 10/27/17 12:36 PM (6 years, 3 months ago) |
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Next recipe will be
200ml WBS soak water 4g Tea 4g Agar
Grain water has known nutrients and is very rich. DO you agree that this 'should' work?
-------------------- Cakes inside Water Tub
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Mateja]
#24741257 - 10/27/17 12:47 PM (6 years, 3 months ago) |
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Yes it has starch in it, which is made from sugars, it will be used to grow and overcome effects. The down side, it increases the chance of an invader of doing the same, and growing.
Personally I think you may be better off using 1-2g of any grain flour. Just so you can measure how much is too much, too little.
You should aim for the minimum needed for growth.
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