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Explicitdetail
Rickshaw Bandit



Registered: 02/03/17
Posts: 592
Loc: North of the Equator
Last seen: 1 month, 8 days
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Re: Cultivation General Discussion [Re: hamloaf]
#24736557 - 10/25/17 12:50 PM (6 years, 3 months ago) |
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Having potency issues? Just eat more...
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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Kyshroomer19xx
Est 19xx soldier


Registered: 04/22/17
Posts: 1,308
Loc:
Last seen: 4 years, 6 months
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Re: Cultivation General Discussion [Re: hamloaf]
#24736558 - 10/25/17 12:50 PM (6 years, 3 months ago) |
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Hey guys I made some agar pasty plates and put some ovoid spores and CRS my question is the agar was pretty solid will they still germinate?
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: hamloaf] 1
#24736570 - 10/25/17 12:53 PM (6 years, 3 months ago) |
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Hey guys, so I've had some not so great looking cultures lately and wanted to try antibacterial agar to see if that would do anything. So after some research I ended up doing black tea agar. 200ml water, 2g dog food, 4g tea and 4g agar. the other three agar recipes are WBS soak water, DFA at 2% and BRF at 2%.
The problem with all this is that none of the cultures are recovering on the black tea agar, I thought mycelium would still grow while the bacteria halts, but was this recipe too deadly even for the mycelium?
On four of these plates is a cube clone and on the other four is Pan Cyan ms.
Cube clone
   
And Pan Cyan MS
   
As you can see the black tea plates have not even started to recover, myc looks dead. Has anyone done black tea agar? I also have green tea, but i read that green tea was much stronger than black tea so I wanted to start out slow. But nothing is growing on the black tea
-------------------- Cakes inside Water Tub
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LemurLemur
Pray for Boog



Registered: 01/30/17
Posts: 6,004
Loc: Drinking on the roof
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Re: Cultivation General Discussion [Re: hamloaf]
#24736594 - 10/25/17 01:02 PM (6 years, 3 months ago) |
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If u do anything do it well.
Every part matters to me from looks to potency...although potency is my main concern so i can watch the rd melt into mercury
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 (when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: LemurLemur]
#24736628 - 10/25/17 01:19 PM (6 years, 3 months ago) |
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Pop quiz. What do you do when you need 24 gallons of horse manure pasteurized by tomorrow?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: hamloaf]
#24736631 - 10/25/17 01:20 PM (6 years, 3 months ago) |
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answer: bucket tek coco instead and say fukkit?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: hamloaf]
#24736638 - 10/25/17 01:22 PM (6 years, 3 months ago) |
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Quote:
hamloaf said: Pop quiz. What do you do when you need 24 gallons of horse manure pasteurized by tomorrow?
Get to fucking work..
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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sir_spins_alot
Feels like a stranger



Registered: 04/04/10
Posts: 640
Loc: United States
Last seen: 6 months, 26 days
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Quote:
Kyshroomer19xx said: Hey guys I made some agar pasty plates and put some ovoid spores and CRS my question is the agar was pretty solid will they still germinate?
Did you actually touch the agar and it felt hard or can you tell by the inoculation loop feeling the agar? My last plates that were stiffer but still clear germinated just fine. How longs it been since you put spores to the plates?
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Buddha19er
New Speedway Boogier'



Registered: 02/09/17
Posts: 252
Loc: Timbers of Fennerio
Last seen: 1 year, 7 months
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Re: Cultivation General Discussion [Re: cronicr]
#24736721 - 10/25/17 01:51 PM (6 years, 3 months ago) |
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I can't knock it till I try it, right? In theory it makes sense, but just adding lemon juice (citric acid) seems like too little effort to be getting the trip reports people give it. I know eating fatty foods like pork before doing Opiates will make them stronger. I was on Opana's for years and know first hand. There is science behind it. I wish there was some more data on this lemon tek. I made one with 1g and one with 2g's of P. Cubensis PE6. I've never taken less than 3.5g of any cube So that is how I'm testing it. Thanks
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"The seeds that were silent all burst into bloom, and decay And night comes so quiet, it's close on the heels of the day..."
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: cronicr]
#24736730 - 10/25/17 01:56 PM (6 years, 3 months ago) |
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Quote:
cronicr said:
Quote:
hamloaf said: Pop quiz. What do you do when you need 24 gallons of horse manure pasteurized by tomorrow?
Get to fucking work..
This is correct, haha. Ya just dook it!
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: hamloaf]
#24736738 - 10/25/17 01:57 PM (6 years, 3 months ago) |
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Nice thread.
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Drayce
Spidget Finner



Registered: 10/07/17
Posts: 260
Loc: Gaia
Last seen: 2 months, 11 days
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Re: Cultivation General Discussion [Re: Ferather]
#24736770 - 10/25/17 02:20 PM (6 years, 3 months ago) |
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Quote:
Ferather said: Nice thread.

Yeah, I enjoy it also.
The premise that fatty things make opiates feel stronger I can attest to. Unlike the idea that one stays high longer if one is filthy, and thus blocked their pores from exuding the drug is IME- hogwash...
But, I'de like to know how the 'experimental lemon tek' thing goes.
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                 “The moment is where we spend most of our time.” – Terence McKenna
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Mateja]
#24736797 - 10/25/17 02:36 PM (6 years, 3 months ago) |
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Quote:
Mateah said: Hey guys, so I've had some not so great looking cultures lately and wanted to try antibacterial agar to see if that would do anything. So after some research I ended up doing black tea agar. 200ml water, 2g dog food, 4g tea and 4g agar. the other three agar recipes are WBS soak water, DFA at 2% and BRF at 2%.
The problem with all this is that none of the cultures are recovering on the black tea agar, I thought mycelium would still grow while the bacteria halts, but was this recipe too deadly even for the mycelium?
On four of these plates is a cube clone and on the other four is Pan Cyan ms.
Cube clone
   
And Pan Cyan MS
   
As you can see the black tea plates have not even started to recover, myc looks dead. Has anyone done black tea agar? I also have green tea, but i read that green tea was much stronger than black tea so I wanted to start out slow. But nothing is growing on the black tea 
Adjusted recipe for you:
100ml boiling hot water, 2g dog food, 4g tea, 1-2g any sugar, 2.8g agar.
No laccase no win, T-Gel will not produce good cubensis growth, and apparently any other dung loving. Your recipe is a little off (very weak), and laccase seems to be out of the question.
Cubensis will mostly run on the proteins in the dog food, gelatin.
Very nice example of the situation, add sugar or starch.

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Side note:
The dog food gelatin, is probably a low quality, and likely weak. You need 240B (bloom) or higher, see amazon here.
Edited by Ferather (10/25/17 03:01 PM)
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Steevo
Just a cog in the death wheel



Registered: 03/02/17
Posts: 2,702
Loc: Here and There
Last seen: 1 year, 9 months
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Re: Cultivation General Discussion [Re: hamloaf] 1
#24736826 - 10/25/17 02:52 PM (6 years, 3 months ago) |
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Quote:
hamloaf said: Pop quiz. What do you do when you need 24 gallons of horse manure pasteurized by tomorrow?
Build a 55 gallon drum sterilizer yesterday?
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Fungus Mountain
Poke-N-Squirt



Registered: 10/16/14
Posts: 1,188
Loc: Front row @ the Freakshow
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Re: Cultivation General Discussion [Re: hamloaf]
#24736849 - 10/25/17 03:04 PM (6 years, 3 months ago) |
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Quote:
hamloaf said:
Quote:
cronicr said:
Quote:
hamloaf said: Pop quiz. What do you do when you need 24 gallons of horse manure pasteurized by tomorrow?
Get to fucking work..
This is correct, haha. Ya just dook it!
-------------------- “Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.” ― George Orwell, 1984 "If you can't explain it simply, you don't understand it well enough." ― Albert Einstein
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Steevo]
#24736916 - 10/25/17 03:24 PM (6 years, 3 months ago) |
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Quote:
Steevo said:
Quote:
hamloaf said: Pop quiz. What do you do when you need 24 gallons of horse manure pasteurized by tomorrow?
Build a 55 gallon drum sterilizer yesterday? 
i swear when i have my own house and property ill have a 55gal drum to steam sterilize massive amounts of grain at once.
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Kyshroomer19xx
Est 19xx soldier


Registered: 04/22/17
Posts: 1,308
Loc:
Last seen: 4 years, 6 months
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No I made 7 pasty plates an 2 got flipped on the side in the PC so I dumped them and touched those and it was pretty stiff I did them on the 23rd 3 ovoids and 2 CRS I can't see any myc growth on any but I am pretty sure I can see contam on the ovoid plates
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Ferather]
#24736954 - 10/25/17 03:36 PM (6 years, 3 months ago) |
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Quote:
Ferather said:
Quote:
Mateah said: Hey guys, so I've had some not so great looking cultures lately and wanted to try antibacterial agar to see if that would do anything. So after some research I ended up doing black tea agar. 200ml water, 2g dog food, 4g tea and 4g agar. the other three agar recipes are WBS soak water, DFA at 2% and BRF at 2%.
The problem with all this is that none of the cultures are recovering on the black tea agar, I thought mycelium would still grow while the bacteria halts, but was this recipe too deadly even for the mycelium?
On four of these plates is a cube clone and on the other four is Pan Cyan ms.
Cube clone
   
And Pan Cyan MS
   
As you can see the black tea plates have not even started to recover, myc looks dead. Has anyone done black tea agar? I also have green tea, but i read that green tea was much stronger than black tea so I wanted to start out slow. But nothing is growing on the black tea 
Adjusted recipe for you:
100ml boiling hot water, 2g dog food, 4g tea, 1-2g any sugar, 2.8g agar.
No laccase no win, T-Gel will not produce good cubensis growth, and apparently any other dung loving. Your recipe is a little off (very weak), and laccase seems to be out of the question.
Cubensis will mostly run on the proteins in the dog food, gelatin.
Very nice example of the situation, add sugar or starch.

----
Side note:
The dog food gelatin, is probably a low quality, and likely weak. You need 240B (bloom) or higher, see amazon here.
So essentially you are suggesting that if I make the same recipe and just add 1-2g of any sugar and add another gram on dog food then the culture would start to grow?
But then how come I have growth and germination even on 1g dog food/100ml water without any other additives? I'm not sure I quite understand what you are telling me here.
And why 2.8% agar instead of 2%?
Edi: Just to make it more clear:
This is 2% Dog Food and works fine, myc not starving.

This is Dog Food at 1% strength (1% gives me similar results in testing, myc not starving) only additive here is the black tea, but when the tea is added then NO growth or recovery at all is visible on the plates. Why?
 
I have a hard time believing that this is because of "weak nutrients" in the agar mix. Or is it something specific about black tea agar that calls for extra sugars or starch to make it work?
Edited by Mateja (10/25/17 03:46 PM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Mateja]
#24737024 - 10/25/17 04:00 PM (6 years, 3 months ago) |
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The extractives in tea are inhibitory, and the mycelium cannot overcome them with decay (laccase). Cubensis will need some added starch-sugar, because it cannot digest the materials.
By weak, I meant the cubensis is not decaying the materials, not fed. The only material it will use, with its enzymes, is protein.
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Ferather]
#24737036 - 10/25/17 04:05 PM (6 years, 3 months ago) |
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Thanks! Now I understand it better! I got the idea from one of your threads, maybe you noticed that
-------------------- Cakes inside Water Tub
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