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InvisibleMoabfighter
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Registered: 12/13/15
Posts: 2,710
Re: Cultivation General Discussion [Re: dankington]
    #23718942 - 10/08/16 10:14 AM (7 years, 3 months ago)

Quote:

dankington said:
If you have a prepped swab, I'd say to go ahead and try to swab 3-4 plates at least. You can only really use it once.





That's true aswell, didn't think about that. To me,TOC is a rare one. I've never been offered it before,never seen it on sponsor sites (could be wrong) anyway I'm excited and glad to have it. I don't want to lose it. Pretty much once I get this agar down, I can keep a "strain" going forever, yes?

Man. I'm stoked. To me this really feels like going from the kiddie pool (MS, PF Tek, etc) to the adult pool. I said when I first started, I don't know shit yet. One day I will get good at this hobby. I feel like those days are somewhere in my near future. Not immediate, but now sooner rather than later. Can't wait.


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KSSS And PE WBS.
 


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OfflineHumbleApprentice
Stranger than most

Registered: 01/09/16
Posts: 2
Loc: PNW
Last seen: 3 years, 2 months
Hello, my first pins... [Re: Moabfighter]
    #23719721 - 10/08/16 02:36 PM (7 years, 3 months ago)

My first post, I wanted to contribute instead of ask a question.
I've been lurking since January. Started P. Cyanescens using PF TEK
and transferred to alder chips until I put them into prepared beds last march.
I've watered and watched them all year and now the PNW is controlling
My environment nicely.
In addition to beds I put some spare mycelium in a flower pot and forgot about it for a while.
The flower pot is first to show pins. I'm pretty stoked. Right now I'm patrolling against slugs.
Perhaps I should set up a few slug taverns around the patches.

My first try at uploading pix, here goes...

H.A.

first shot is mycelium on wood chips



My first pin!!!


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InvisibleInocuole
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Re: Cultivation General Discussion [Re: HumbleApprentice]
    #23719888 - 10/08/16 03:46 PM (7 years, 3 months ago)

Interesting.. don't see many Ps Cyanescens grows.  Gonna go ahead and.. steal the subject line back.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisibleMoabfighter
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Posts: 2,710
Re: Cultivation General Discussion [Re: Inocuole]
    #23720119 - 10/08/16 05:20 PM (7 years, 3 months ago)

I have a PS cyan syringe. IIRC I can't grow them indoors right?


--------------------
KSSS And PE WBS.
 


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InvisibleMoabfighter
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Posts: 2,710
Re: Cultivation General Discussion [Re: Moabfighter]
    #23720319 - 10/08/16 06:37 PM (7 years, 3 months ago)

Okay so I've made agar.... lol now what?

I have one ready to use swab of Texas orange cap, a lot of syringes of many strains, and a very few prints. I'm so excited. I don't know where to start. I want the "easiest" most "successful" route.  Hell, I even have some PF shrooms growing, I could take chunks from.  I'm so excited.

I've been "growing" for a year now. I have a decent head on my shoulders for someone who's only grown PF Tek style. I feel like the sky is now endless with this agar going. I'm just looking for the best way to dip my feet in the pool.


--------------------
KSSS And PE WBS.
 


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InvisibleComebackKid
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Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23720969 - 10/08/16 11:12 PM (7 years, 3 months ago)

Moab.
Get a craft knife (scalpel) from walmart or art store. Use a butane torch lighter to sterilize the tip. Scrape some spores off your print and wipe them onto agar.

Or break open the stem of a nice fruit from pf grow. Tease some tissue from inside with your knife and place it on agar.

Watch myc and bacteria grow. Cut clean wedges of agar containing mushroom myc from one dish and transfer to another. Repeat untill you only see clean myc. Then drop wedges of clean myc agar into jars of grain or LC


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleMoabfighter
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Posts: 2,710
Re: Cultivation General Discussion [Re: ComebackKid]
    #23720989 - 10/08/16 11:31 PM (7 years, 3 months ago)

Pulled one plate out of the PC to see what I have to work with. Slightly unlevel, and what appears to be serious condensation ....

Fatality mortal kombat style or am I gonna be alright? Kinda drunk right now so. Not gonna fuck with tonight, but tomorrow I plan to start some big big things...

I'm wasted, but my excitement level hasn't gone Dow. Any that's for sure :laugh:


--------------------
KSSS And PE WBS.
 


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23721158 - 10/09/16 02:21 AM (7 years, 3 months ago)

I dank a 26 tonight, but all I cn say is condensation is to be expected ith pasty plates
Its a bitchm but after a while it works itself out
Condensation occurs because of a temperature difference..
I find that if i leave the plates in the PC until it cools off that there is much less to wory about

another user likes to use 4oz canning jars and lets the condensation form into water and then pours i5 out before trnsfers


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineHuskies
Boop More Snoots
I'm a teapot


Registered: 03/22/16
Posts: 1,048
Last seen: 1 day, 6 hours
Re: Cultivation General Discussion [Re: ComebackKid]
    #23721552 - 10/09/16 09:29 AM (7 years, 3 months ago)

So I recently did my first agar transfers, i made 4 new plates from plates A and B. B plates are doing much better, and I think I will do new transfers with the best sectors to some new ones.

But after those are done, what is the current best use of agar for grain jars? Liquid Inoculate? Or is just wedges to jars then g2g more efficient?


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I call them Huskies cause you tell them to go "Mush! Mush""


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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,342
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Re: Cultivation General Discussion [Re: Huskies]
    #23721574 - 10/09/16 09:40 AM (7 years, 3 months ago)

Liquid Inoculate


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Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot

Registered: 07/06/16
Posts: 988
Re: Cultivation General Discussion [Re: Moabfighter]
    #23721717 - 10/09/16 10:39 AM (7 years, 3 months ago)

I like to roll with a slightly altered recipe than Pasty describes in his tek.
I use this recipe for clones and transfers.

My Potato Dextrose Agar goes like this:

1/2 cup water
2.5 grams agar
1.5 tsp. potato flakes
3-4 drops of food coloring

In my experience, this slightly less nutritious solution gives me more aggressive mycelial growth - without starving the mycelium.

Pasty's recipe in his tek is an awesome place to start-I just like my growth to be a tad bit faster as my temperature in my storage area sits around 65-68f. Pasty's recipe is where I begin when taking spores to agar as the more nutritious media helps with spore germination.

However, even at normal room temperature, I find I have better experiences with a slightly less nutritious recipe than what was initially described by Pasty in his no pour tek.

I edited this post a bit because after Pasty commented below-I felt like I was coming off a bit too asshole-ish...like I might be saying his recipe 'isn't right'... this is most certainly not the case.
I am simply sharing that in my own experience-I have had luck with deviating from the recipe slightly and lowering the nutrient level when I am not working with spore germination and working with transfers and clones.

:cheers:


Edited by morty422 (10/09/16 11:06 AM)


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: Cultivation General Discussion [Re: morty422]
    #23721727 - 10/09/16 10:42 AM (7 years, 3 months ago)

The recipe i outline is meant to help with spore germination, hence the high nutes. I do state that it's important to rotate the media in the writeup, it was never intended to be the only recipe a person uses.


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Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot

Registered: 07/06/16
Posts: 988
Re: Cultivation General Discussion [Re: Pastywhyte]
    #23721764 - 10/09/16 10:59 AM (7 years, 3 months ago)

Quote:

Pastywhyte said:
The recipe i outline is meant to help with spore germination, hence the high nutes. I do state that it's important to rotate the media in the writeup, it was never intended to be the only recipe a person uses.




Yes of course - I definitely alter the media type every two or three transfers. Also, I do understand the higher nutrient level in your PDA is to help with spore germination.

I was not trying to say that your recipe is not a valid one or anything, in fact I used your exact recipe for around 130-150 plates with no issues once-so-ever...

I just feel that the recipe I stated above has been working a bit better for me as of late than the one you outlined at first.

I still stand by my comment that your recipe you stated is a good place to start.

I hope I didn't come off as someone who was trying to say your no-pour tek is not 'good enough' or anything.

I will edit my post so that other users understand what I mean a bit more.

Hah. You're a plethora of knowledge Pasty - I owe you more than you know.


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Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot

Registered: 07/06/16
Posts: 988
Re: Cultivation General Discussion [Re: ComebackKid]
    #23721794 - 10/09/16 11:12 AM (7 years, 3 months ago)

Quote:

ComebackKid said:
I dank a 26 tonight, but all I cn say is condensation is to be expected ith pasty plates
Its a bitchm but after a while it works itself out
Condensation occurs because of a temperature difference..
I find that if i leave the plates in the PC until it cools off that there is much less to wory about

another user likes to use 4oz canning jars and lets the condensation form into water and then pours i5 out before trnsfers




I agree-however, I have still YET to get rid of condensation from fresh PC'd no pour. It always clears up in a few days though-so it's not that big of a deal to me.


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InvisibleMoabfighter
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Registered: 12/13/15
Posts: 2,710
Re: Cultivation General Discussion [Re: morty422]
    #23721822 - 10/09/16 11:23 AM (7 years, 3 months ago)

So I swiped three plates with a swab earlier. I have 5 plates left that haven't been unfoiled. I'm accounting for say 1 plate to be a complete disaster. No real reason why, it just was my first run of agar and I wouldn't be surprised if I get less than pro results.

Is 5 empty plates enough for me to clean say two plates up with? Do I need to make more?


--------------------
KSSS And PE WBS.
 


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Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot

Registered: 07/06/16
Posts: 988
Re: Cultivation General Discussion [Re: Moabfighter]
    #23721843 - 10/09/16 11:31 AM (7 years, 3 months ago)

Quote:

Moabfighter said:
So I swiped three plates with a swab earlier. I have 5 plates left that haven't been unfoiled. I'm accounting for say 1 plate to be a complete disaster. No real reason why, it just was my first run of agar and I wouldn't be surprised if I get less than pro results.

Is 5 empty plates enough for me to clean say two plates up with? Do I need to make more?




Maybe, I would do more. You never know. I also like to take multiple pulls from each culture so with 3 swabs- you can easily take 2 wedges from each-thats six already. If you immediately recycle the old ones you could still potentially use more. I would do another 8 and have more room for play!

:cheers:


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: morty422]
    #23721875 - 10/09/16 11:44 AM (7 years, 3 months ago)

20 plates per go is a good amount for me.

Spores to 4-5 plates. The rest to clean up


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: bodhisatta]
    #23722208 - 10/09/16 01:15 PM (7 years, 3 months ago)

If you have a nice growth but a spot of mold in the corner can you still transfer from that plate?  Its the last decent culture from a clone.


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Edmunter]
    #23722819 - 10/09/16 04:35 PM (7 years, 3 months ago)

Will pan cyans do alright in the 60's or should i keep them warmer


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Edmunter]
    #23722825 - 10/09/16 04:36 PM (7 years, 3 months ago)

Quote:

Edmunter said:
If you have a nice growth but a spot of mold in the corner can you still transfer from that plate?  Its the last decent culture from a clone.



I would transfer and then transfer 3 more times to make sure its fine


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