|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Cagney
Hanging with my Shroomy's


Registered: 09/12/16
Posts: 332
Last seen: 3 years, 11 months
|
|
Speaking of properly treated substrates, here lately I have been having problems with contamination. It seems the last dozen or so tubs have been triching out on me either during or right after the first flush. It generally happens between the 14 to 20th day. I thought at first it was bad spawn but for it to continually keep happening and narrowing down vectors I can't be positive. I have been using the "elementary coir" tek. I'm not using a flow hood, in the past I used a SAB in order to perform all my work, agar, and inoculation of grain jars. I Have always used WBS. I switched this round and decided to try using a glove box to do all my agar work, and inoculating my jars. I had a good success rate with my agar. I think I lost about 8 plates out of 90, most the contamination came from the ms syringe. I knocked up 72 quarts over the past two weeks, all of which show no signs of contamination just yet. I am wondering if the problem is in my substrate prep. I use a 5 gallon bucket. Any ideas to help me solve this problem would be greatly appreciated. I get most my weight off first flush anyways, but like the monsters that come with the following flushes. Currently I have three tubs of PE in the middle of their first flushes which are all blobs. Two tubs are already showing small rings of contamination. Bout to pull my hair out.
--------------------
"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Cultivation General Discussion [Re: Cagney]
#24551151 - 08/13/17 04:18 PM (6 years, 5 months ago) |
|
|
Grab some manure and pasteurize and you will know if it's your sub prep...
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 11 hours
|
Re: Cultivation General Discussion [Re: cronicr]
#24551231 - 08/13/17 04:48 PM (6 years, 5 months ago) |
|
|
or just pasteurize what you have. bucket tek isn't proper pasteurization, that could be why you are having troubles.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
|
abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
|
Re: Cultivation General Discussion [Re: Tookitooki]
#24551239 - 08/13/17 04:50 PM (6 years, 5 months ago) |
|
|
thanks tooki, I'd like to get some more on the go, but I'm at a crossroad of " keep going" and " stop again" .. not sure if its intuition or paranoia. :p
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Cultivation General Discussion [Re: tryptkaloids]
#24551473 - 08/13/17 06:39 PM (6 years, 5 months ago) |
|
|
Quote:
tryptkaloids said: or just pasteurize what you have. bucket tek isn't proper pasteurization, that could be why you are having troubles.
With coir...no.
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re: Cultivation General Discussion [Re: cronicr]
#24551595 - 08/13/17 07:13 PM (6 years, 5 months ago) |
|
|
I disagree. I've seen trich on the top of my coir casing layer with spawn that was definitely clean. There's shit in eco earth that will cause contamination, no doubt in my mind.
--------------------
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Cultivation General Discussion [Re: natedawgnow]
#24551720 - 08/13/17 08:04 PM (6 years, 5 months ago) |
|
|
Now explain how pasturizing would help...
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re: Cultivation General Discussion [Re: cronicr]
#24551728 - 08/13/17 08:07 PM (6 years, 5 months ago) |
|
|
Didn't say it would. Just saying that I don't agree that coir is always contam proof as is usually suggested here
Sure I'm gonna receive some flak for saying so but just callin it like I see it.
--------------------
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Cultivation General Discussion [Re: natedawgnow]
#24551735 - 08/13/17 08:09 PM (6 years, 5 months ago) |
|
|
I'm not saying that either...I'm saying weather you pasteurize sterilize or bucket tek coir makes no diff...it lack the beneficial microbes so if there are impurities it doesn't matter.
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
|
Re: Cultivation General Discussion [Re: cronicr]
#24551776 - 08/13/17 08:22 PM (6 years, 5 months ago) |
|
|
How do you guys get your whatman filters to stick to your lids? I used silicon on a metal lid, but they pop loose when i pc them. Silicon is high temp. What your tricks?
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
|
Re: Cultivation General Discussion [Re: cronicr]
#24551786 - 08/13/17 08:25 PM (6 years, 5 months ago) |
|
|
Generally it's a seed that's in the coir that will contam.
I see what your saying but like cron said is true. You would probably be making it more susceptible to contams.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re: Cultivation General Discussion [Re: van hatton]
#24552001 - 08/13/17 09:28 PM (6 years, 5 months ago) |
|
|
I think it's possible that, in some cases and possibly some batches of coir, proper pasteurization may be necessary. I've argued plenty on here before that bucket tek or full sterilization is fine for coir, but after seeing trich on the top of a 1/2" casing layer on a few tubs from the same batch of coir I'm starting to think otherwise.
I know spawn was clean because it only contammed on the bucket coir and nothing else. I work in front of my hood and I've poured hundreds of plates in front of my hood and haven't had one contam yet due to a bad pour or transfer, jars are sterilized at near 20psi for 2 hours and spawn recolonizes quick after a shake and looks amazing.
It's not every tub but a couple and it's always on the top of the casing. The fact that it's summer and temps are high and my state has a high spore load probably plays a major role, but I just am no longer convinced that coir is AS contam proof as usually suggested, although it is pretty damn contam proof.
--------------------
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Cultivation General Discussion [Re: natedawgnow]
#24552051 - 08/13/17 09:57 PM (6 years, 5 months ago) |
|
|
I'm not sure that coir has the microbes for pasturizing to be effective
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
|
Re: Cultivation General Discussion [Re: cronicr]
#24552055 - 08/13/17 10:00 PM (6 years, 5 months ago) |
|
|
You can fortify coir with thermophiliac bacteria by laying it out in the sun on a tarp for a week, or so. https://www.shroomery.org/forums/showflat.php/Number/24506925#24506925 Compressed coir isn't going to have much in the way of any microbes due to the high pressure heat, and exposure to said high pressure heat it takes to get coir into that compressed brick form.
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re: Cultivation General Discussion [Re: cronicr]
#24552059 - 08/13/17 10:02 PM (6 years, 5 months ago) |
|
|
I would normally agree with you, but then I thought about the swampy nature of the areas in which they are made. Sure the intense heat during compaction kills microbes, but then how long do the blocks sit around before they get packaged and shipped out? Is there a warehouse where it all sits? I mean I'm sure some of it gets stockpiled and sits for a while before going out and I think those blocks have the potential to be micro-biologically active. I doubt the health code standards of their facilities are really that strict
I don't think it's the case 100% of the time, but I think it can definitely be a possibility.
--------------------
|
pyro101981
pyro101981


Registered: 02/06/15
Posts: 288
Loc: united states
Last seen: 6 years, 4 months
|
Re: Cultivation General Discussion [Re: Tookitooki] 1
#24552072 - 08/13/17 10:08 PM (6 years, 5 months ago) |
|
|
I found this out the hard way, scuff your lid before you apply silicone
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Cultivation General Discussion [Re: pyro101981]
#24552099 - 08/13/17 10:19 PM (6 years, 5 months ago) |
|
|
It is thermophillic bacteria that is needed...and it's not something that just happens to land on something and magic happens....it is an organism that requires a lot of heat which is why manure is so rich in it as it gets very hot while things like fire flange colonize it.it's a very interesting process actually
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
|
Re: Cultivation General Discussion [Re: cronicr]
#24552114 - 08/13/17 10:28 PM (6 years, 5 months ago) |
|
|
Nah you are incorrect. The outdoors is ripe with the microlife kingdom, so if you lay coir out in the sun on a tarp microlife of both natures are allowed to land on it and begin colonizing. There's nothing "magical" about it. You are also incorrect about thermophiliac bacteria. Thermophiliac bacterial is thermophiliac, because it can with stand, and in fact thrive in higher temperature ranges that our competitor bacteria can't. This is the main reason pasteurization is affective. The temperature increase causes the thermophiliac bacteria to thrive while killing off, and, or knocking back competitor bacteria. Again. No magic involved.
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Cultivation General Discussion [Re: hamloaf]
#24552127 - 08/13/17 10:35 PM (6 years, 5 months ago) |
|
|
Lol your saying exactly what I was ...
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Cultivation General Discussion [Re: cronicr]
#24552130 - 08/13/17 10:36 PM (6 years, 5 months ago) |
|
|
Yes keep coir damp outdoors for a bit it becomes very full of life...but not so much sitting on a dry shelf.
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
|