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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: Adden]
#24413036 - 06/17/17 04:53 PM (6 years, 7 months ago) |
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Quote:
Adden said: If fruiting at spawning works for you just keep doing it.
It does. But never when I've cased. Always seems great, then Trichs out two weeks in. Uncased though I always seem to get atleast a flush.
-------------------- KSSS And PE WBS.
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: Cultivation General Discussion [Re: Moabfighter]
#24414119 - 06/18/17 04:21 AM (6 years, 7 months ago) |
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I always case at spawn.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Cultivation General Discussion [Re: liloldme]
#24414264 - 06/18/17 06:54 AM (6 years, 7 months ago) |
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What do you use for a casing? How do you prepare it?
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: Kenetic] 1
#24414294 - 06/18/17 07:26 AM (6 years, 7 months ago) |
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I use the same stuff from my bucket Tek. Starting to wonder if it's the water. Every time I spray my roaches container it gets trich on the coco where the water lands. Had been using spring water but ran out of it.
The only other variable would be bad technique. But really, dropping a plate to a jar in a sab shouldn't be that hard.
-------------------- KSSS And PE WBS.
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: Moabfighter]
#24414319 - 06/18/17 07:48 AM (6 years, 7 months ago) |
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Ahh. Just found some green on one /7 shoeboxes.
My desire to keep doing this is quickly diminishing. Playing with Fire for green mold farm with a touch of trippy myc inside. Crazy man. About to give up.
Used squeaky clean from master PE clone plate wedge, tiny wedge, into cooked grain water. 2 seconds of movement. And I got green. There's just no way. Nothing's passing over open medium, nothing. Open plate, tiny wedge, move 6 inches to jar lid, lift lid, slide in and seal. That's it. It's not THAT hard, I don't know why I keep going green.
The only thing I can come up with is bucket Tek isn't working. I don't fucking know but this blows.
-------------------- KSSS And PE WBS.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: Moabfighter] 1
#24414355 - 06/18/17 08:06 AM (6 years, 7 months ago) |
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If you really think it's the bucket then give proper pasteurization a go. It's Not that hard and you'll know for sure
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: natedawgnow]
#24414363 - 06/18/17 08:09 AM (6 years, 7 months ago) |
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Quote:
natedawgnow said: If you really think it's the bucket then give proper pasteurization a go. It's Not that hard and you'll know for sure
I will look into it. I just don't know man. Is it the micropore pasty plates letting hidden contams in? Should I try filtered half pint agar plates? Don't really have enough lids for all that... I just want to get it. I've been trying agar and WBS/oats for over a year now, still can't nail it. Got a big ole PC. New SAB. Real filters. Did BRF for almost a year before that. So I'm almost two years in, and still don't have it down. I'm not stupid. Pretty smart guy. This is stumping me though.
-------------------- KSSS And PE WBS.
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: Moabfighter]
#24414410 - 06/18/17 08:25 AM (6 years, 7 months ago) |
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Just checked a jar I top fruiting from different strain (KSSS) in no way correlates to the PE I was talking about earlier.

Mold.
Spawned same day as the PE. Same CVG used on the moldy PE tub. Also used same untreated well water to mist with. This jar was not broken up. Cased and open air fruited with Coke lid over jar. I suspect jar two of the KSSS will have mold shortly.
I'm not trying to be joe in his basement closet growing 5 shroomz in my SGFC. I have an entire room of my house dedicated to this. I've been hanging onto very little for almost two years, not quit yet, because this is the hobby I want to excel at and put my time into. I enjoy it when it rarely works. It's often just one disappointment after the next, and I do not know why. I have all the stuff minus a flowhood. I have crazy shit, respirators and hazmat suits, on top of thinking about every move I make very thoroughly and planned. I ask you all questions on even the most minute details. Sucks it's not working out.
-------------------- KSSS And PE WBS.
Edited by Moabfighter (06/18/17 08:37 AM)
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Moabfighter] 1
#24414631 - 06/18/17 09:46 AM (6 years, 7 months ago) |
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The reason you are having a hard time in the beginning is becasue you are meant for greatness. Tribulation tuffens you up and puts little myco hairs on your chest. I grew nothing but green my first year or so, but I was noccing spores with LC, so go figure. Took a while to figure that one out haha. Point is, now I can grow blind folded with both hands tied behind my back. Failure is the stepping stones to success.
Build or buy a small flow hood since you have a room to dedicate. Start back at spores and generate new cultures. The six vectors to the mushroom culture are;
The inoculum. The media. The cultivator. The air. The tools. Mobile Contamination units (such as pests, and pets).
Hope that helps. Good luck.
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AtomHeart
MK Ultra Operative



Registered: 05/05/06
Posts: 397
Last seen: 3 years, 1 month
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Re: Cultivation General Discussion [Re: Moabfighter] 1
#24414662 - 06/18/17 10:00 AM (6 years, 7 months ago) |
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Quote:
Moabfighter said:
Quote:
Adden said: If fruiting at spawning works for you just keep doing it.
It does. But never when I've cased. Always seems great, then Trichs out two weeks in. Uncased though I always seem to get atleast a flush.
Based on those descriptions of timelines on the contam, I would scrutinize your grain prep, and maybe even try changing the source of your grains, and see if you see any change. If you're 100% sure your grain game is tight, maybe see if pasteurizing your casing or casing with sterilized straight vermiculite changes anything...and case late.
-------------------- You once ripped a man's jaw off...I seent it!
Edited by AtomHeart (06/18/17 10:07 AM)
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LemurLemur
Pray for Boog



Registered: 01/30/17
Posts: 6,004
Loc: Drinking on the roof
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Re: Cultivation General Discussion [Re: hamloaf]
#24414665 - 06/18/17 10:02 AM (6 years, 7 months ago) |
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O.O
--------------------
 (when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Cultivation General Discussion [Re: hamloaf]
#24414671 - 06/18/17 10:04 AM (6 years, 7 months ago) |
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You use well water? Maybe.....
I would just use spring water from the store. I personally use tap water but the water here is good.
I don't know why youre having such a hard time man but I imagine its frustrating.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Cultivation General Discussion [Re: Kenetic] 2
#24415002 - 06/18/17 12:24 PM (6 years, 7 months ago) |
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Had a guy here who used well water and there was too much copper in it (anti fungal). His family was falling ill, and when his mushrooms kept dying he had it tested and kept his wife and two kids out of the hospital. Definitely check your well water. Streak some to agar right out the tap see what happens. Run side by side with an open air one from around your sink area and cross off duplicates see if you can isolate something in your water. I mean I doubt it but we had some kittens die after getting some protozoa disease from the water.. you never know. We never got sick but once the cats started dying we contacted the landlord and state immediately and made landlords pay for all the water we had to buy in the interim.
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morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
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Re: Cultivation General Discussion [Re: Moabfighter]
#24415988 - 06/18/17 07:25 PM (6 years, 7 months ago) |
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Glad to see your name again Moab!
Don't give up... I recently had to push back to spores due to failure after failure. All is well now...
Good luck brother!
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: morty422]
#24416154 - 06/18/17 08:34 PM (6 years, 7 months ago) |
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Quote:
morty422 said: Glad to see your name again Moab!
Don't give up... I recently had to push back to spores due to failure after failure. All is well now...
Good luck brother!
I wish I could fix it because I wanna know what the deal is. I'm strongly thinking it has to be the water that I mist with and/or bucket Tek with.
I finally had literal palm break up spawn a few weeks ago, three jars I made with LC straight from a plate of clean myc from a friend, looked the most perfect ever, smacked twice with my hand and they broke up. Spawned the following week into a mini mono. Got trich two weeks in. If easy busting spawn is success, add me to the statistic that didn't succeed.
Gonna go buy a few cases of spring water and try again. Anything I've been using probably shouldn't Keep going with. I made 7 shoeboxes, one quart in each. 1-1.5 substrate max. Some cased, some not. One uncased showed green mold today. Will check tomorrow and see if I find more. All have been misted with untreated well water straight from the sink.
-------------------- KSSS And PE WBS.
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 14 hours, 52 minutes
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Re: Cultivation General Discussion [Re: Moabfighter]
#24416259 - 06/18/17 09:17 PM (6 years, 7 months ago) |
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Hey Moab...Pasty suggested running a heat treated vermiculite substrate to see if its the spawn or another vector like the coir.
Water is definately a vector for all sorts of stuff too. I had to stop using my creek water without boiling it first and still do only because i'm too lazy to change a a UV filter bulb.
--------------------

Shadowboxing the apocalypse and wandering the land
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Thufir_Hawat
Mentat


Registered: 03/18/17
Posts: 399
Last seen: 4 days, 4 hours
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Re: Cultivation General Discussion [Re: Thufir_Hawat]
#24416357 - 06/18/17 10:12 PM (6 years, 7 months ago) |
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Quote:
Thufir_Hawat said: Thoughts about this novelty/experiment? Inoculated a few random substrates from MSS back on the 21st (see bare notes in my journal entry ) and the only one that took off other than agar and BRF was this oatmeal:

Tried to get a good look all around the jar, single inoculation point, squirted on top of the cooked and PC'd oatmeal through a SHIP, has a tyvek 1/4" GE port in the center of the lid.
It started very quickly, around 5 days after inoculation I noticed some very faint mycellium on the side of the jar, and a small white blob in the center. Visibility has been next to nil thanks to the mycellium on the walls and the solid lid. From what little I can make out, the entire top is covered in white growth. I'm super curious, but obviously don't want to open it yet.
Opened it up inside a new bag, using an alcohol soaked paper towel to clean the jar and bag in addition to opening the lid with it.
Very hard to get a picture that captures how it looks, due to the bag on top and the walls of the jar (which are covered in mycellium), but it looks gorgeous to me.

Pure white, big sort of rolling hill formations in there, need to set up a light and macro it tomorrow.
Next to a quart of grain from agar inoculated with the same spores as the oatmeal:
Edited by Thufir_Hawat (06/18/17 10:12 PM)
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gnubeus

Registered: 04/30/17
Posts: 4
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Re: Cultivation General Discussion [Re: Moabfighter]
#24416441 - 06/18/17 10:53 PM (6 years, 7 months ago) |
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Quote:
Moabfighter said: I use the same stuff from my bucket Tek. Starting to wonder if it's the water. Every time I spray my roaches container it gets trich on the coco where the water lands. Had been using spring water but ran out of it.
The only other variable would be bad technique. But really, dropping a plate to a jar in a sab shouldn't be that hard.
Are you 100% sure that the coir you're using hasn't been pretreated with trichoderma?
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Cultivation General Discussion [Re: gnubeus]
#24416607 - 06/19/17 12:56 AM (6 years, 7 months ago) |
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got some kind of hidden contam in this ms plate
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Cultivation General Discussion [Re: Boogieman47]
#24416844 - 06/19/17 04:07 AM (6 years, 7 months ago) |
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@moab
lots of great advice on this page
i think adden is on the right track. scrutinize your grain prep, its almost certainly that.
a few things you can try to figure it out for sure:
#1 make a small tray of grain and try to fruit it directly, see if it contams
#2 dont be fooling with LC until you get this worked out. LC is very finicky, use LI if you must, but i would just a2g and g2g
#3 try some trays/tubs uncased and see what they do
#4 try a 50/50+ casing, properly pasteurize (roaster oven is as easy as it gets)
#5 try a different batch of grain. i have encountered grain that contamed no matter WHAT i did
#6 try different coir
#7 can try different water, but i kinda doubt thats what it is
if you control for each variable, you will figure it out for sure
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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