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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: jkz]
#24373115 - 06/03/17 08:22 AM (6 years, 7 months ago) |
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Quote:
jkz said: What does everyone do with their agar plates that have spores germinated on them after you take transfers?
i keep them in the sab until i use em. toss in a old control jar of grains. both would go in the trash anyway.
results can be meh but sometimes they surprise you which is fun.
old germ plate+old jar=

weird shit mang
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jkz
Padawan


Registered: 06/16/13
Posts: 1,910
Loc: Coruscant
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Re: Cultivation General Discussion [Re: mushboy]
#24373118 - 06/03/17 08:23 AM (6 years, 7 months ago) |
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After you've taken transfers tho?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: jkz]
#24373119 - 06/03/17 08:24 AM (6 years, 7 months ago) |
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Cultivation General Discussion [Re: jkz] 1
#24373120 - 06/03/17 08:24 AM (6 years, 7 months ago) |
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Garbage? IDK what you wanna know lol. The way I see it you have 2 options. Transfer to new, clean plates for fun, or toss.
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jkz
Padawan


Registered: 06/16/13
Posts: 1,910
Loc: Coruscant
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Re: Cultivation General Discussion [Re: mushboy]
#24373126 - 06/03/17 08:28 AM (6 years, 7 months ago) |
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Gotcha. Not a bad idea. Better then tossing it in the trash. Might even get a few fruits while I'm waiting for more transfers to grow out.
All the previous transfers ive taken look good so far. Even the one i touched with my finger lol tho i dont have high hopes for the future of that one. I can't get a sensible picture tho.
How many transfers do you take before fruiting to test for a good culture?
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dhype773
Enter the Void



Registered: 10/29/15
Posts: 2,182
Loc: Valhalla
Last seen: 6 years, 2 months
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Re: Cultivation General Discussion [Re: jkz]
#24373131 - 06/03/17 08:31 AM (6 years, 7 months ago) |
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Oh, my bad. I've only germinated spores once. And it was clear that there was bacteria present. So I choose healthy growth to make my transfers and threw the rest of the plates out in a straw pile in my back yard for the hell of it.
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jkz
Padawan


Registered: 06/16/13
Posts: 1,910
Loc: Coruscant
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Re: Cultivation General Discussion [Re: dhype773]
#24373137 - 06/03/17 08:33 AM (6 years, 7 months ago) |
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Makes sense dhype. The plates I have colonizing with spores look pretty clean, so I was wondering what if anything I could do with it. I don't like wasting shit honestly. So if I could do anything useful with it I would. That's all.
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Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
Last seen: 4 months, 29 days
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Re: Cultivation General Discussion [Re: jkz]
#24373156 - 06/03/17 08:40 AM (6 years, 7 months ago) |
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You are a microbiologist at this point. You will always waste as we are dealing with billions and millions in number most of the time.
You could make one Ms plate and from that 100s. Kind of the name of the game. That's why I use mason jars for 90% of my agar work. Don't feel like im wasting so much when i don't have so many plastic petris to fuck off.
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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dhype773
Enter the Void



Registered: 10/29/15
Posts: 2,182
Loc: Valhalla
Last seen: 6 years, 2 months
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Re: Cultivation General Discussion [Re: jkz]
#24373158 - 06/03/17 08:42 AM (6 years, 7 months ago) |
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Once they've germinated, you have mycelium. So, if you are certain they are clean, no need to throw them out
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: dhype773]
#24373162 - 06/03/17 08:44 AM (6 years, 7 months ago) |
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i wouldnt keep everyplate. i throw out plenty. but the old GERM plates have tons of genetics.
sometimes you get some winners.
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dhype773
Enter the Void



Registered: 10/29/15
Posts: 2,182
Loc: Valhalla
Last seen: 6 years, 2 months
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Re: Cultivation General Discussion [Re: mushboy]
#24373171 - 06/03/17 08:51 AM (6 years, 7 months ago) |
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Yeah, I'm sure I'll reach the point if isolating genetics for more desirable performance sooner or later, but for now I just want to keep some tasty sacrements on hand for consciousness expansion lol.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: dhype773]
#24373183 - 06/03/17 08:57 AM (6 years, 7 months ago) |
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If there's not much contamination were talking very minimal I fridge them so I don't have to start from spores again as they are already germinated.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Re: Cultivation General Discussion [Re: Crispykoot]
#24374460 - 06/03/17 06:08 PM (6 years, 7 months ago) |
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Quote:
Crispykoot said: Any thoughts on what size and brand impulse sealer might be good to have on a spawn making bench?
12 inch American International Electric. It will last a lifetime
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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he is talking about an impulse sealer, not your favorite sex toy dill 
i really dig my foot operated one i got from uline a long time ago. the smaller ones might be better for our purposes since it could fit in a sab or fh, but for sealing LOTS of bags it doesnt get any easier than the foot pedal, its really handy for any kind of packaging
i almost bought a band sealer back in the day to do some contract manufacturing of health food products, got that foot operated impulse sealer instead
to use it, i put some wood under it to raise it up into the flow of my FH. if i am particularly worried about it i will sometimes use a food saver to seal it inside the FH, then do like 5 or 6 more seals with the impulse sealer
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: Cultivation General Discussion [Re: c10h12n2o]
#24374507 - 06/03/17 06:26 PM (6 years, 7 months ago) |
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I like mine. It holds itself shut until the seal cools off.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Cultivation General Discussion [Re: liloldme]
#24374515 - 06/03/17 06:30 PM (6 years, 7 months ago) |
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ooooo i havent seen one like that, thats awesome is that a magnet?
what model is that?
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 3 years, 7 months
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Re: Cultivation General Discussion [Re: van hatton]
#24374527 - 06/03/17 06:34 PM (6 years, 7 months ago) |
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Quote:
van hatton said: If there's not much contamination were talking very minimal I fridge them so I don't have to start from spores again as they are already germinated.
This is a good move for the typical hobbyist. Have grown many shrooms doing it this way. Instead of tossing out good mycelium. But you could literally take old plates from previous generations of transfers and if you feel bad tossing it cause you think it's good, than toss that whole plate in a jar of grains it'll probably do it's job.
But some people cant afford the time to waste on measely transfer plates.
I've always been really efficient with time spent vs moves made to get the most for what im putting in which i'll admit is not as much time as I wish I could put in.
But ideally you want to toss out all plates you intend to transfer from after use and also be in front of a hood or in an SAB every other day using fresh growth from many spore swabs if you want more genetics. Instead of fridging old already transferred and potentially compromised specimen. Thats how things can get sloppy. Being deliberate with your p values and exposure times with cultures is what really guarantees success.
-------------------- AMU Q&A
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Cultivation General Discussion [Re: SloppyJoseph]
#24374600 - 06/03/17 06:59 PM (6 years, 7 months ago) |
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i used to track p values, then i realize that you arent even supposed to start counting until its an isolate. also that stamets is basically the only person in the field who uses p value. so i stopped
i wonder what the p value of Humongous Fungus is ? lol (it could be 8000 years old)
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: c10h12n2o]
#24374716 - 06/03/17 07:46 PM (6 years, 7 months ago) |
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Only reason I do that is in case my transfer hit a road block of contamination somewhere.
My goal isn't much an isolation but rather a slanted clone of car. Or w.e the fuck it's called.
I usually throw out all of my dishes after use. The only ones that might get saved are the intial swab or the 1st transfer. Depending on the amount of contamination present.
Sides that I just made some lc lids with a hook for ease of removal without compromising the lc inside. I got the idea from some thread here so thanks whoever you are
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: spacechildo]
#24375235 - 06/03/17 11:53 PM (6 years, 7 months ago) |
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Quote:
spacechildo said: search anastomosis and cloning etc stare, I've seen that question asked a bunch myself but never any clear answer, long story short anastomosis can make the different sectors exchange info so it doesnt matter if you isolate away certain sectors. then again there's no other way to know whether it took place or not than growing out a culture and see what grows (or doesnt grow)
I'm familiar with the concept of anastomosis, but usnure as to how long it takes for the sectors to exchange the information. I've read repeatedly that you can isolate away from the fruiting genetics so it looks like there's not an immediate fusion of these genetics. That's why I was asking if it's best to let a clone plate grow out a bit so the mycelial network has a chance to exchange genetics throughout and form connections, etc.
Just more curious about the shit that's happening than a practical issue. I can take clones and fruit them, either way.
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