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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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kuntsman
Stranger
Registered: 06/02/17
Posts: 2
Last seen: 6 years, 7 months
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Re: Cultivation General Discussion [Re: mynakedrat]
#24370150 - 06/02/17 08:17 AM (6 years, 7 months ago) |
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Quote:
mynakedrat said: Yes those will be a crazy amt of pins
Thanks. Is it likely that all the pins will progress to caps?
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: kuntsman] 1
#24370159 - 06/02/17 08:23 AM (6 years, 7 months ago) |
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Quote:
kuntsman said:
Quote:
mynakedrat said: Yes those will be a crazy amt of pins
Thanks. Is it likely that all the pins will progress to caps?
Yeah if you quit playing with it.
-------------------- KSSS And PE WBS.
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AtomHeart
MK Ultra Operative



Registered: 05/05/06
Posts: 397
Last seen: 3 years, 1 month
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Re: Cultivation General Discussion [Re: Moabfighter]
#24370164 - 06/02/17 08:28 AM (6 years, 7 months ago) |
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If you pick it, it will never heal.
-------------------- You once ripped a man's jaw off...I seent it!
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: Crispykoot]
#24370190 - 06/02/17 08:45 AM (6 years, 7 months ago) |
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Quote:
Crispykoot said: Some really interesting information here about Trich and how it is effected by sterilization/pasteurization etc...
The more you know, the more you grow...
www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400017
3rd sentence in the abstract is where I go  and stop reading.
"Mushroom growers are usually worried about the contaminations that may occur after these treatments during handling or spawning."
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: spacechildo]
#24370228 - 06/02/17 09:01 AM (6 years, 7 months ago) |
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Alpha420Zeus
ShroomCurious

Registered: 11/06/16
Posts: 19
Last seen: 6 years, 6 months
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Re: Cultivation General Discussion [Re: mushboy]
#24370250 - 06/02/17 09:11 AM (6 years, 7 months ago) |
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Quick question, what substrate would you recommend for a rye tek spawn?
Was thinking of doing a 1:2.5 spawn to sub ratio.
Opinions? Advice? Much appreciated.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Crispykoot] 1
#24370267 - 06/02/17 09:18 AM (6 years, 7 months ago) |
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Quote:
Crispykoot said: Some really interesting information here about Trich and how it is effected by sterilization/pasteurization etc...
The more you know, the more you grow...
www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400017
Read the whole thing and all it says is you have to properly pasteurize substrates that we all agree & say routinely that you have to properly pasteurize
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: spacechildo]
#24370277 - 06/02/17 09:20 AM (6 years, 7 months ago) |
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Quote:
spacechildo said:
Quote:
Crispykoot said: Some really interesting information here about Trich and how it is effected by sterilization/pasteurization etc...
The more you know, the more you grow...
www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400017
3rd sentence in the abstract is where I go  and stop reading.
"Mushroom growers are usually worried about the contaminations that may occur after these treatments during handling or spawning."
They are if using straw it can contaminate after spawning if that substrate wasn't prepared correctly.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#24370312 - 06/02/17 09:40 AM (6 years, 7 months ago) |
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Quote:
bodhisatta said:
Quote:
spacechildo said:
Quote:
Crispykoot said: Some really interesting information here about Trich and how it is effected by sterilization/pasteurization etc...
The more you know, the more you grow...
www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400017
3rd sentence in the abstract is where I go  and stop reading.
"Mushroom growers are usually worried about the contaminations that may occur after these treatments during handling or spawning."
They are if using straw it can contaminate after spawning if that substrate wasn't prepared correctly.
I still dont believe not getting your straw properly prepared is what "mushroom growers are usually worried about". maybe if you buy your spawn but people should worry more abuot their spawn.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: spacechildo]
#24370326 - 06/02/17 09:46 AM (6 years, 7 months ago) |
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Farms are different. You have some grunts doing your spawn prep and then well paid lab making spawn.
Also using 1:10 spawn to substrate ratio the substrates like straw have a much longer window of opportunity to become contaminated before ran over with mycelium
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#24370341 - 06/02/17 09:51 AM (6 years, 7 months ago) |
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OK, I see your point there, but how is anything in that link gonna help us here avoid the green? If you get it in your spawn you're fucked anyway, you cant PC or hotbath your spawn like they suggest you do with your subs.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: spacechildo] 1
#24370353 - 06/02/17 09:55 AM (6 years, 7 months ago) |
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All that link did is reiterate what we already believe and say
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 18 hours, 27 minutes
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Re: Cultivation General Discussion [Re: bodhisatta]
#24370365 - 06/02/17 09:58 AM (6 years, 7 months ago) |
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I have read all the relevant stuff. There is a LOT that can be applied to coir. Farms vary greatly in how they operate...Point here is that there are many vectors when it comes to Trich. Spawn is likely not the greatest one from what I'm reading. Sterilization and even pasteurization even for long duration doesn't always kill Trich.
There's more to it all. Its been greatly oversimplified.
--------------------

Shadowboxing the apocalypse and wandering the land
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Crispykoot]
#24370374 - 06/02/17 10:00 AM (6 years, 7 months ago) |
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Coir is different nothing they said really applies to coir
Pasteurization kills trich. They added trich back in on purpose to see how well pasteurization worked at defending against it
Sterilization always kills trich
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Myco Mako
He who speaks with fungus



Registered: 03/13/17
Posts: 687
Loc: Mars
Last seen: 6 years, 6 months
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Re: Cultivation General Discussion [Re: Crispykoot]
#24370378 - 06/02/17 10:01 AM (6 years, 7 months ago) |
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Quote:
Crispykoot said: Improper sterilization and even improper pasteurization even for long duration doesn't always kill Trich.
Fixed it for you.
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 18 hours, 27 minutes
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Re: Cultivation General Discussion [Re: Crispykoot]
#24370395 - 06/02/17 10:05 AM (6 years, 7 months ago) |
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"Reports vary on the susceptibility of T. aggressivum to heat treatment; in vitro test have shown that 60 ℃ for 9 hr is sufficient to kill T. aggressivum (Morris et al., 2000), other researchers state that T. aggressivum can often survive industry standard pasteurisation to some extent (Beyer et al., 2000; Rinker & Alm, 2000). A recent report showed that while standard pasteurisation at 60 ℃ for 12 hr will reduce T. aggressivum to undetectable levels, but reduction in the temperature to 57 ℃ and time to 8 hr makes pasteurisation considerably less effective (Grogan et al., 2012). Local temperature fluctuations in the order of a few degrees within a large mass of substrate are conceivable, so it may be the case that sub-optimal pasteurisation could lead to a reduction, rather than removal of T. aggressivum from the substrate which may not become apparent until the later stages of production, when T. aggressivum proliferates."
Maybe PH up the water at heat treatment...Don't cook the coir as much like in a cooler? IDK
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Shadowboxing the apocalypse and wandering the land
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Crispykoot] 1
#24370408 - 06/02/17 10:07 AM (6 years, 7 months ago) |
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If you want your cultivation knowledge to come from out of context research that's clearly over your head go for it lol
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: abductee]
#24370675 - 06/02/17 11:34 AM (6 years, 7 months ago) |
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Quote:
abductee said: Take different sectors, a,b,c for example, let those sectors grow out in their own plate and then take a wedge from each plate and put that to spawn. Grow it out and see which isolate was best a, b, or c.
You are not grasping what I am asking. I obviously know how to get to an isolate. I'm asking ham personally what his protocol is, specifically, if he's transferring out each sector and when, because I feel like it makes a difference due to anastomosis. Transferring every single sector would be tine consuming, so he may just pick the fastest, etc. I'm just curious.
For clones, I am asking about keeping a clone in tact for longterm use. An isolate from the clone is NOT always a better choice as it's been shown that certain clone's perform better with other sets of genetics than separated, IIRC.
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 18 hours, 27 minutes
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Any thoughts on what size and brand impulse sealer might be good to have on a spawn making bench?
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Shadowboxing the apocalypse and wandering the land
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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You said you were afraid of getting genetics that dont fruit from a plate with many sectors/ genetics. If you try and fruit each sectors you take and label them ad example: a, b, or.c. You will see wich " isolate" fruits. Maybe ham knows how to tell before hand.lol but that is all I can think of. I believe clones often produce fruits.
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