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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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My experience with growing out clones is non existent, but I believe I remember reading that the clones genetics are set, and you won't get any other genetics from that mushroom unless you collect its spores. I'm not 100 percent sure, but I'm sure if someone will correct me. I think only factor with clone isolates is taking a transfer that is further from any contams if there are any on the plate.
Edited by abductee (06/01/17 04:26 AM)
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dhype773
Enter the Void



Registered: 10/29/15
Posts: 2,182
Loc: Valhalla
Last seen: 6 years, 2 months
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Re: Cultivation General Discussion [Re: abductee]
#24366961 - 06/01/17 05:14 AM (6 years, 7 months ago) |
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I think you are right. That is why you have to go to print after x amount of generations from the same clone. Otherwise senescence sets in, and the genetics die. Something along those lines I think. I'll be finding out soon enough, gaining my own experience.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: abductee]
#24367030 - 06/01/17 06:17 AM (6 years, 7 months ago) |
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Quote:
abductee said: My experience with growing out clones is non existent, but I believe I remember reading that the clones genetics are set, and you won't get any other genetics from that mushroom unless you collect its spores. I'm not 100 percent sure, but I'm sure if someone will correct me. I think only factor with clone isolates is taking a transfer that is further from any contams if there are any on the plate.
Nah, with clones, if they are from a multi spore grow the fruits are set with several genetics. Mycelium straight from clone will still have several families/strains of mycelium. Mycelium straight from clone once in a while will bust a single sector isolate but it's not very common.
The last sentence doesn't make sense. I think you mean if you clone from pinhead, the myce will be multiplying/running so fast that if you take leading edge transfers of a culture of that nature that the myce has a chance of out running any contams present. You have to take the transfers before the culture hits the walls, though, or the contams will catch up.
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Explicitdetail
Rickshaw Bandit



Registered: 02/03/17
Posts: 592
Loc: North of the Equator
Last seen: 1 month, 8 days
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Re: Cultivation General Discussion [Re: hamloaf]
#24367220 - 06/01/17 08:16 AM (6 years, 7 months ago) |
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Okay so I have a question for all you agar gurus out there. So I keep running into this issue when doing Agar to agar transfers. I'm able to get my colonized agar piece onto my scalpel pretty easily now but every time I go to transfer it to my new dish I keep running into this issue where the piece I'm trying to transfer will keep making its way up my scalpel blade instead of actually transferring into the new dish. I'm not sure if this is because the pieces I'm trying to transfer are to large or maybe the consistency of my agar isn't ideal. Has anyone else ran into this problem and have any possible advice. Thanks in advance.
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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jkz
Padawan


Registered: 06/16/13
Posts: 1,910
Loc: Coruscant
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I had the exact same problem last night. Seems to be a skill related issue. What are you using for a scalpel? I found putting my blade on its side and kinda shipping it onto the new plate to work. Or cutting into the new agar to get the old piece off onto the new plate.
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Explicitdetail
Rickshaw Bandit



Registered: 02/03/17
Posts: 592
Loc: North of the Equator
Last seen: 1 month, 8 days
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Re: Cultivation General Discussion [Re: jkz]
#24367237 - 06/01/17 08:30 AM (6 years, 7 months ago) |
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It's a number 11 replaceable blade on a handle. I tried moving the blade every way I could ynink of to get it off and no matter what I did it's just seemed to move further up the blade until it was either at the un-flamed portion or it cut in two and a piece feel off.
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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i use the same blades. i use the flat area of the blade to collect my sample. then into the recieving media i flip the blade and mash the transfer tissue onto the plate.
ive seen the vids where people just poke and place the tissue and im like how he do that?
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AtomHeart
MK Ultra Operative



Registered: 05/05/06
Posts: 397
Last seen: 3 years, 1 month
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I cut the wedge, then slide my scalpel down into the cut and pry upwards gently while sliding the blade into the side of the wedge slightly...then lift out of the donor dish, lowering the lid with my left hand. Move left hand to the recieving dish lid and lift to about a 45 degree angle...I then rotate and tip the scalpel with wedge up and lay it face down on the recieving plates agar while pulling out the scalpel. This not only gets the mycelium side down against the media, but creates some friction to hold the wedge as the scalpel is pulled out, but the real trick is making sure when stabbing the wedge, you just stab in enough to barely hold it for the later dropping maneuver. If you skewer it like a kabob, it can be an absolute bitch to get off in the recieving plate!
-------------------- You once ripped a man's jaw off...I seent it!
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Explicitdetail
Rickshaw Bandit



Registered: 02/03/17
Posts: 592
Loc: North of the Equator
Last seen: 1 month, 8 days
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Re: Cultivation General Discussion [Re: AtomHeart]
#24367258 - 06/01/17 08:42 AM (6 years, 7 months ago) |
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Quote:
AtomHeart said: I cut the wedge, then slide my scalpel down into the cut and pry upwards gently while sliding the blade into the wedge slightly...then lift out of the donor dish, lowering the lid with my left hand. Move left hand to the recieving dish lid and lift to about a 45 degree angle...I then tip the scalpel with wedge up and lay it face down on the recieving plates agar while pulling out the scalpel. This not only gets the mycelium side down against the media, but creates some friction to hold the wedge as the scalpel is pulled out, but the real trick is making sure when stabbing the wedge, you just stab in enough to barely hold it for the later dropping maneuver. If you skewer it like a kabob, it can be an absolute bitch to get off in the recieving plate!
Ahhh I can already tell I was probably placing my scalpel in to far cause I was kabobbing the shit out of my little pieces. Also the mycelium face down was something I wasn't aware of, ill be sure to give this a try. Thanks for the advice
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

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jkz
Padawan


Registered: 06/16/13
Posts: 1,910
Loc: Coruscant
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Yeah you want to try to get the myc facing down it seems. I ended up with some facing up, some down. Even pushed the sample off the scalpel with my finger on one dish. Terrible practice but I was curious how dirty my finger was lol
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AtomHeart
MK Ultra Operative



Registered: 05/05/06
Posts: 397
Last seen: 3 years, 1 month
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Re: Cultivation General Discussion [Re: jkz]
#24367265 - 06/01/17 08:45 AM (6 years, 7 months ago) |
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Haha. You'll know soon.
-------------------- You once ripped a man's jaw off...I seent it!
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Explicitdetail
Rickshaw Bandit



Registered: 02/03/17
Posts: 592
Loc: North of the Equator
Last seen: 1 month, 8 days
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Re: Cultivation General Discussion [Re: AtomHeart]
#24367278 - 06/01/17 08:54 AM (6 years, 7 months ago) |
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Yea I agree with Mushboy though I watch some of the vids people have posted and they put zero effort and it just falls off perfectly into place. My plates looks like freddy krueger had a seizure by the time I finally get my piece off.
-------------------- "Problems that remain persistently insoluble should always be suspected as questions asked in the wrong way."

Edited by Explicitdetail (06/01/17 08:55 AM)
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jkz
Padawan


Registered: 06/16/13
Posts: 1,910
Loc: Coruscant
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Re: Cultivation General Discussion [Re: AtomHeart]
#24367289 - 06/01/17 09:01 AM (6 years, 7 months ago) |
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Quote:
AtomHeart said: Haha. You'll know soon.
Oh I figure I will. The rest of my transfers were done properly. The last one tho, gave me shit so I said fuck it lol
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion *DELETED* [Re: Sporepoise]
#24367296 - 06/01/17 09:04 AM (6 years, 7 months ago) |
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Quote:
Sporepoise said: Post deleted by Sporepoise<p>Reason for deletion: Answer found.
Yea don't do that anymore
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dhype773
Enter the Void



Registered: 10/29/15
Posts: 2,182
Loc: Valhalla
Last seen: 6 years, 2 months
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Re: Cultivation General Discussion *DELETED* [Re: bodhisatta]
#24367306 - 06/01/17 09:11 AM (6 years, 7 months ago) |
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Someone needs to make a new gif. "The Bod Hammer"
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Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
Last seen: 4 months, 29 days
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Re: Cultivation General Discussion *DELETED* [Re: dhype773]
#24367395 - 06/01/17 09:54 AM (6 years, 7 months ago) |
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Pour thicker agar lol. That's singularly the fix. The mass is not enough to combat the water tension between the blade and the agar....
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Cultivation General Discussion [Re: hamloaf]
#24367664 - 06/01/17 11:47 AM (6 years, 7 months ago) |
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Thanks for clearing that up hamington. 
I just did some grain to grain with different master jars, one was a clone i labeled short and thick , short being about 6 inches. Looking forward to see the results.
Edited by abductee (06/01/17 11:49 AM)
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Cultivation General Discussion [Re: abductee]
#24367678 - 06/01/17 11:54 AM (6 years, 7 months ago) |
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I noticed my casing was rising, and I was wondering if I banged it or something, but no.. I think it's a stone pushing up.lol
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zajcob
Stranger

Registered: 07/28/10
Posts: 208
Last seen: 6 years, 3 months
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Re: Cultivation General Discussion [Re: abductee]
#24368848 - 06/01/17 07:05 PM (6 years, 7 months ago) |
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My metal inoculation loops keep melting/ disintegrating when I flame them! Has anyone else had this happen to them?
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Cultivation General Discussion [Re: zajcob]
#24368937 - 06/01/17 07:44 PM (6 years, 7 months ago) |
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I just got a torch recently and havent used my loop yet. What are you using for a loop? A guitar string might withstand the heat.
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