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InvisibleMoabfighter
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Registered: 12/13/15
Posts: 2,710
Re: Cultivation General Discussion [Re: Boogieman47]
    #23668011 - 09/22/16 12:55 PM (7 years, 4 months ago)

Eek. Not much room for trial and error there haha. May have to get more than that.
So. Very basically, I put spores on agar, they grow out, and the contams. I take a chunk of mycelium from a nice clean area, put it on new plate. New plate grows out. That's then clean myc? I can inoculate a jar with a tiny sliver of myc?


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KSSS And PE WBS.
 


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InvisibleMunchauzen
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23668019 - 09/22/16 12:58 PM (7 years, 4 months ago)

I personally use 2 grams of agar per 10 plates.


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23668025 - 09/22/16 01:00 PM (7 years, 4 months ago)

Ya basically if you use proper sterile technique and your syringe aint dirty you should be good but always want 2 to 3 transfers to ensure cleanliness ... look up agar agar sticks they are always cheaper they're 20 grams for 1.30


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23668038 - 09/22/16 01:04 PM (7 years, 4 months ago)

Found 15g from seaweed solutions for 6.50. Think that sounds like a great deal and it's pay day. May order this evening. I add instant potato flakes to it? Like loterally Idaho spuds?


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KSSS And PE WBS.
 


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23668046 - 09/22/16 01:06 PM (7 years, 4 months ago)

Thats the shit i use 15 grams for 6.50 isnt that great but if you cant find any cheaper then go for it i jusy bought 400 grams for 30 bucks at my asian mart


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InvisibleInocuole
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23668047 - 09/22/16 01:06 PM (7 years, 4 months ago)

Quote:

Moabfighter said:
Found 15g from seaweed solutions for 6.50. Think that sounds like a great deal and it's pay day. May order this evening. I add instant potato flakes to it? Like loterally Idaho spuds?




How could you possibly think that's a great deal...

Quote:


Seeing 5 packs of telephone brand for 10 bucks.




5 packs of telephone is 125g.  That's over 500 plates easy.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23668162 - 09/22/16 01:48 PM (7 years, 4 months ago)

Quote:

Moabfighter said:
Looking for agar substitutes if anyone knows any.

Found this very simple tek on here using water and BRF. That's it.

https://www.shroomery.org/8514/Agar-substitute




Do yourself a favour and just order it online man. Even just a pack of 50gs powdered will last you a while.
I couldnt find it cheap and will have to keep an eye out but after working with it for a short time I have decided that clean spawn is priceless.
I couldnt imagine switching to this poverty tek after working with agar. You could have mold and bacteria growing anywhere under the top layer of "clean" myc and you wouldnt even know.

This tek does have me thinking though... Would it not have the same effect to just make a batch of jello and, before letting it harden in the frige, spoon a few tbs of jello in some pasty plates and pc?


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleInocuole
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Re: Cultivation General Discussion [Re: ComebackKid]
    #23668235 - 09/22/16 02:12 PM (7 years, 4 months ago)

No... Jello is always liquid at room temperature.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Inocuole]
    #23668265 - 09/22/16 02:20 PM (7 years, 4 months ago)

Then i was wrong earlier i thought a sugar free jello mix would work but i did forget jello melted haha


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23668274 - 09/22/16 02:25 PM (7 years, 4 months ago)

Jello melts? Hmm, jello melts... :justdontknow:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineAVShroomer
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Registered: 05/19/03
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23668287 - 09/22/16 02:29 PM (7 years, 4 months ago)

Hello shroomery family! I have a quick question.
  I knocked up 11 jars 30 days or so ago. They kinda stalled growth and started speeding up last week when i kept my tote cracked open instead of shut up tight. My question is the first cake is 100% today but it looks like all my cakes will be fully colonized at different times the next 8 days or so at the rate its going now will it be ok to dunk and roll each cake one by one once they are done with the 7 days consolidation? or should I wait till I get a few 100%?? if i do that the first cake might be almost two weeks into consolidating. What would the best thing to do be? All the cakes look great they are just going a bit slow they are BRF cakes that are B+ strain.


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'It's not a war on drugs its a war on personal freedom'
>**My Trip Journal**<


Edited by AVShroomer (09/22/16 02:32 PM)


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: AVShroomer]
    #23668344 - 09/22/16 02:48 PM (7 years, 4 months ago)

Best thing would be to let them consolidate for 2 weeks or until yoy see signs of pinning. But there really is no problem at all with fruiting individually at different times. Rarely will your cakes all finish at the same time.

I personally like to wait for each jar to show signs of pinning before I birth but a weeks consolidation will do just fine as well.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleNumeroEno
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Re: Cultivation General Discussion [Re: ComebackKid]
    #23668357 - 09/22/16 02:50 PM (7 years, 4 months ago)

I replied to an old post again :facepalm:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
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Re: Cultivation General Discussion [Re: AVShroomer]
    #23668361 - 09/22/16 02:51 PM (7 years, 4 months ago)

Anybody ever have fluffy centers of their myc like this? I feel like it's because my chunks are bigger than normal due to pouring my plates thicker, but want to make sure.



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InvisibleNumeroEno
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Re: Cultivation General Discussion [Re: stareatclouds]
    #23668364 - 09/22/16 02:53 PM (7 years, 4 months ago)

Yep. That's just the myc growing through and out of the piece you transferred.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflineAVShroomer
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Registered: 05/19/03
Posts: 832
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Re: Cultivation General Discussion [Re: ComebackKid]
    #23668385 - 09/22/16 02:58 PM (7 years, 4 months ago)

Quote:

ComebackKid said:
Best thing would be to let them consolidate for 2 weeks or until yoy see signs of pinning. But there really is no problem at all with fruiting individually at different times. Rarely will your cakes all finish at the same time.

I personally like to wait for each jar to show signs of pinning before I birth but a weeks consolidation will do just fine as well.




  Thank you for the response! Ok also would it be a good idea after its 100% consolidated to take it out and put it out in the open on a table so it gets some in direct light from the sun shining in my house or should I just keep it in the dark untill I put it in my sgfc? I have looked up that question and have found mixed responses.


--------------------


'It's not a war on drugs its a war on personal freedom'
>**My Trip Journal**<


Edited by AVShroomer (09/22/16 02:59 PM)


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: AVShroomer]
    #23668427 - 09/22/16 03:08 PM (7 years, 4 months ago)

about to pc some qtips is this look ok to you guys? 45 minute cycle


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: AVShroomer]
    #23668479 - 09/22/16 03:25 PM (7 years, 4 months ago)

Hmm Im not quite sure what your asking but sunlight is always good no matter what you're doing. I hope youre not colonizing those jars in the dark!


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: ComebackKid]
    #23668548 - 09/22/16 03:50 PM (7 years, 4 months ago)

Ive got 3 pe swabs giveaway in the marketplace left


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23668567 - 09/22/16 03:55 PM (7 years, 4 months ago)

Do you have to sterilize your qtips before swabbing? Seems like overkill considering youre swabbing a dirty mushroom anyway.
One more question.
Is this the only way to obtain PE spores? Or can you make prints somehow?


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


Extras: Filter Print Post Top
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