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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: Cultivation General Discussion [Re: Boogieman47]
    #24285678 - 05/01/17 12:29 AM (6 years, 8 months ago)

I did a thin layer across the bottom of a couple of my wide mouth pints that I use for my agar.


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InvisibleTheMadHatter420
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Registered: 10/12/16
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24285679 - 05/01/17 12:30 AM (6 years, 8 months ago)

Up till now everything has germinated for me. But they elude me lol.


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24285682 - 05/01/17 12:31 AM (6 years, 8 months ago)

Damn those are some tempermental spores haha have you tried a warm pour ??


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InvisibleTheMadHatter420
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Re: Cultivation General Discussion [Re: Boogieman47]
    #24285684 - 05/01/17 12:32 AM (6 years, 8 months ago)

that I have not yet.


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24285690 - 05/01/17 12:33 AM (6 years, 8 months ago)

Just pc some agar and wait till it looks about to solidify but still on the more liquidy side and pour a thin layer over the agar with spores on it


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InvisibleTheMadHatter420
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Registered: 10/12/16
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Re: Cultivation General Discussion [Re: Boogieman47]
    #24285696 - 05/01/17 12:36 AM (6 years, 8 months ago)

I will have to give it a try. I was going to swipe a bunch more plates when I got through some of these other strains. I'm just looking for a couple good cultures that will keep me happy right now.


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Offlinesharpie95
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Re: Cultivation General Discussion [Re: Boogieman47]
    #24285700 - 05/01/17 12:37 AM (6 years, 8 months ago)

Should i be worried if i see no fruits yet... Put in mono on 4/14. One tub has matted.. Theyre in a 16qt tub. Coir only. 2 1/2 spawn in each tub.


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InvisibleTheMadHatter420
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Posts: 12,941
Re: Cultivation General Discussion [Re: sharpie95]
    #24285702 - 05/01/17 12:38 AM (6 years, 8 months ago)

pics would help, but so long as nothing has gone green don't panic just yet.


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24285710 - 05/01/17 12:43 AM (6 years, 8 months ago)

Ya thats where im at on my clones ... my ksss, pe, and illusion weavers plus the mazzie which i deleted the ladt pic i had .... those weavers were actually not thst great but i didnt take care of the tub


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Offlinesharpie95
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Re: Cultivation General Discussion [Re: Boogieman47]
    #24285717 - 05/01/17 12:48 AM (6 years, 8 months ago)



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InvisibleTheMadHatter420
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Registered: 10/12/16
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Re: Cultivation General Discussion [Re: sharpie95]
    #24285722 - 05/01/17 12:50 AM (6 years, 8 months ago)

ok I don't like that yellow spot, but that's a shit load of knots that will be full on pins VERY soon.


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Offlinesharpie95
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24285734 - 05/01/17 12:56 AM (6 years, 8 months ago)

I know i dont like it either :frown: .. Should i just let it be? What do u think that is..?


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: sharpie95]
    #24285739 - 05/01/17 12:59 AM (6 years, 8 months ago)

Its metabolites it looks to me like a small pool of water take a paper towel and dab it with the corner ... if it is a dry spot then dont worry about it it will be fine


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InvisibleTheMadHatter420
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Re: Cultivation General Discussion [Re: Boogieman47]
    #24285748 - 05/01/17 01:09 AM (6 years, 8 months ago)

Let them bitches go, they are about to shit fruits.


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Invisiblec10h12n2o
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24285815 - 05/01/17 02:35 AM (6 years, 8 months ago)

So I've been reading, and it's actually electrical\ionic triggers that make spores germinate

Was reading some studies where they actually electrocute both spores and myc, for various ends!

I'm gonna fuck around with some of these ideas just for the hel of it haha...

Not that it'll probably help you any


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: c10h12n2o]
    #24285816 - 05/01/17 02:36 AM (6 years, 8 months ago)

Theres actually a dude on here that made some like tesla towers around his tubs thinking they will grow better ill try to find it


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Boogieman47]
    #24285820 - 05/01/17 02:43 AM (6 years, 8 months ago)



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Offlinesharpie95
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Re: Cultivation General Discussion [Re: Boogieman47]
    #24287022 - 05/01/17 03:25 PM (6 years, 8 months ago)

I have poured 20 plates in a sab.. 1 went bad. Stored 10 others I didnt used. Saran wrapped. Then used my sab to inoculate.. Couple days later those 10 all had trich. Im wondering if it has to do with using my sab with the lid? Does it matter? I misted, covered the lid and let it sit for 20min and went to work. Flammed needle. Made sure nothing hovered the plates as i dropped two drips from a MS. Maybe i should flip the sab downwards with no lid and try a towel? Lmk .. Im started to get disapponted with all these failures :frown:


Edited by sharpie95 (05/01/17 03:26 PM)


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Offlinehamloaf
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Re: Cultivation General Discussion [Re: sharpie95] * 1
    #24287045 - 05/01/17 03:32 PM (6 years, 8 months ago)

Your inoculum may have been dirty.  If not it's your technique. 

You have to keep going through the motions, though, man, if you want to win!

Pay critical attention to what you are doing, and try to figure out where you may have went wrongs with your technique. 

Keep trying though.  In the beginning, this hobby is meet with a lot of failure.  Remember:  Failure is a stepping stone to success.


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OfflineAtomHeart
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Re: Cultivation General Discussion [Re: sharpie95]
    #24287060 - 05/01/17 03:38 PM (6 years, 8 months ago)

Quote:

sharpie95 said:
I have poured 20 plates in a sab.. 1 went bad. Stored 10 others I didnt used. Saran wrapped. Then used my sab to inoculate.. Couple days later those 10 all had trich. Im wondering if it has to do with using my sab with the lid? Does it matter? I misted, covered the lid and let it sit for 20min and went to work. Flammed needle. Made sure nothing hovered the plates as i dropped two drips from a MS. Maybe i should flip the sab downwards with no lid and try a towel? Lmk .. Im started to get disapponted with all these failures :frown:




Aww man, I know how you feel.  I would bet almost everyone here has been to that point before.  Hang in there.  You will have success...its just a difficult thing, doing sterile things an a non-sterile environment.

It's hard to tell from your description how you did the inoculation but, I try to just lift the lid of the petri with my left hand to a slight angle above the agar, and move the scalpel, needle, loop, or whatever I'm using with my right hand just under the lid, do my business and then move back out while lowering the lid.  The lid never gets more than an inch or so away from the surface of the agar, and I do everything with very slow, smooth motions. 

That has always worked well for me.  If that sounds like just what you did, my apologies.


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Edited by AtomHeart (05/01/17 04:09 PM)


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