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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 1 month
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Re: Cultivation General Discussion [Re: Inocuole]
#24265402 - 04/23/17 01:40 AM (6 years, 9 months ago) |
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: hamloaf]
#24265448 - 04/23/17 02:14 AM (6 years, 9 months ago) |
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Quote:
hamloaf said:
Quote:
stareatclouds said: ham,
Are there holes in the bottom of that tote or nah?
Yessir. Thanks for making sure. That's good looking out.
For sure! I figured you were on top of it, but couldn't see holes in the pic.
Quote:
How about a large mouth (90mm) plastic lid as a saucer? Place the lids on the perlite upside down, and place the cakes into the lids. Should make bottom watering a breeze. Do you guys think that the cakes will get consolidated enough to even fit inside of a 90mm lid?
I'm unfamiliar with bottom watering and I've only done cakes once for my first grow, but I thought foil was preferred because you can bend it downward to run off water and avoid pooling. It seems like this isn't the goal with bottom watering, though.
Excited to see the results with this!
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hamloaf
Loaf of Fam.

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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This experiment is a couple of weeks out, but has begun as of about 10 minutes ago with the agar warming up in the sterilizer.
The APE being featured in this research is from a nice, live culture on agar. It's a little old, and has been stored in a refrigerator, so we'll se if it will take to a few transfers. Finger crossed.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: hamloaf]
#24265671 - 04/23/17 06:51 AM (6 years, 9 months ago) |
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I can't wait to see as I had been under the impression that APE doesn't do so well on cakes.
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Just_A_Noob
Breathing



Registered: 12/30/16
Posts: 6,809
Loc: PNW
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Yeah, inoculating with squirt guns is so last year... Just kiddin, but I really do want to launch some azurescens bombs!!!
-------------------- Wearing a mask is bad for my physical, emotional, and spiritual health. Complying = Consent Wide Mouth 1/2 Pint No-Pour TEK TC Teks & Links
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Just_A_Noob]
#24266792 - 04/23/17 05:10 PM (6 years, 9 months ago) |
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So I have had some issues with moisture levels in mono tubs. I hope I get it right this time. I use Ecoearth coir, mixed 3 brick with 11QT of water. Its mixed nicely, I only get a small stream of water if I squeeze as hard as I can. Is that still to much to be considered field capacity or should I be good. I mean I have to give it a really hard squeeze to get the small stream. If some of you think it mays till be to much I got my trust vermiculite to adjust moisture content. Please and thanks for any help guys.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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You're making the sub on the dry side. I add 4'5 quarts of water per brick and 3 qts of verm, perfect every time. I can think of at least 3 good cultivators here who add 5 qts of water using eco earth and it's still good.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Josex]
#24266833 - 04/23/17 05:26 PM (6 years, 9 months ago) |
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Really, damn. I didn't have issues with some coir bricks from a sponsor but do with ecoearth. From what I seen on RR video about field capacity I should be about right. I just have had issues with to much moisture and fruits like these. Didn't have this before ecoearth,(only did 6 tubs with 3 bricks before ecoearth) and think I had to much water in there. Also I didn't put any vermiculite in it yet, just straight coir.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Oh true, didn't read that you aren't using verm. Say Bod three times out loud to summon it, he uses only coir most of the time IIRC.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Josex]
#24266863 - 04/23/17 05:44 PM (6 years, 9 months ago) |
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It honestly has a really nice feel to it. I just don't have much vermiculite and would rather save it for my pseudo-casing.
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
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Quote:
TheMadHatter420 said: It honestly has a really nice feel to it. I just don't have much vermiculite and would rather save it for my pseudo-casing.
Make sure to heat treat the verm too...I'm sure you are on it...I'm reading this fairly baked.
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Shadowboxing the apocalypse and wandering the land
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Crispykoot]
#24266915 - 04/23/17 06:19 PM (6 years, 9 months ago) |
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I never used to heat treat the vermiculite. It has no nutritional value so what is the idea behind this? To kill potential spores laying dormant in it? Around the same time I switched to Ecoearth I decided to give up casing as so many say it is not needed.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Crispykoot]
#24266930 - 04/23/17 06:24 PM (6 years, 9 months ago) |
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I've done rez effect in a shoebox using verm right out of the bag, no heat treatment whatsoever, no contams. You really don't need to treat verm.
Didn't like the results, but that's beside the point.
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
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Re: Cultivation General Discussion [Re: Josex]
#24266937 - 04/23/17 06:28 PM (6 years, 9 months ago) |
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Thats probably true...I just always throw it in the cooler with the C and the G and heat treat the whole thing.
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Shadowboxing the apocalypse and wandering the land
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Crispykoot]
#24266942 - 04/23/17 06:31 PM (6 years, 9 months ago) |
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Me too. But I treat CVG because it improves the texture of the sub and makes it easier for the myc to colonize it, not really trying to kill anything here.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: Crispykoot]
#24266949 - 04/23/17 06:32 PM (6 years, 9 months ago) |
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I sprinkle just enough to cover my substrate and mist it until it is hydrated. It's such a thin layer that it works out fine like this. Before I would get thicker casings that seemed to compact down.
Yea I heat treat my sub mix to "cook" the coir, I just don't worry about the verm I use for the thin casing layer.
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
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Re: Cultivation General Discussion [Re: Josex]
#24266954 - 04/23/17 06:35 PM (6 years, 9 months ago) |
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I heat treat to kill all the motherfucking things that will die and it improves the texture and breaks it down...
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Shadowboxing the apocalypse and wandering the land
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
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Re: Cultivation General Discussion [Re: hamloaf]
#24266959 - 04/23/17 06:37 PM (6 years, 9 months ago) |
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Quote:
hamloaf said: This experiment is a couple of weeks out, but has begun as of about 10 minutes ago with the agar warming up in the sterilizer.
The APE being featured in this research is from a nice, live culture on agar. It's a little old, and has been stored in a refrigerator, so we'll se if it will take to a few transfers. Finger crossed.
Is this actual research or "internet research" ?
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Shadowboxing the apocalypse and wandering the land
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: Crispykoot]
#24268301 - 04/24/17 11:29 AM (6 years, 9 months ago) |
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For whatever reason I'm not the best with distinguishing bacteria and clean myc on my plates....
Anyway, I do have some clean jars, with clean leftover grains. I figure the best way to keep it going would be to plop a clean grain on a plate, make one transfer, then use those to inoculate with. I haven't seen this done too often, and I'm curious as to why not?
Also, by doing this, is this keeping the same genetics that was from the original jars, and this method can keep those same genetics going forever?
Thanks for the help.
-------------------- KSSS And PE WBS.
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hamloaf
Loaf of Fam.

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Moabfighter]
#24268845 - 04/24/17 04:43 PM (6 years, 9 months ago) |
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They won't be the same genetics unless you're working with an isolate, and the mycelium won't keep going forever.
Inculcated 3 LC's today. 1 Beech oyster mushroom, a Lion's Mane, and Pearl oyster.
3 no pour agar squat jars were inoculated with live APE culture from a clone that's around a year old, and has been stored in a refrigerator.
Inoculated 2 no pour squat jars with some 4 year old print marked "Pan Cyan Tampa". Made a spore syringe with the remainder of the prints in an attempt to rehydrate the spores.
2 no pour squats got inoculated with ATL#7 spores.
Finally, 3 squat no pours were inoculated with a year, and a half old Pan Cyan Goliath print.
Here's to hoping for a high recovery/germination rate of mushroom mycelium.
Edited by hamloaf (04/24/17 05:08 PM)
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