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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Cultivation General Discussion [Re: Boogieman47]
    #24261672 - 04/21/17 12:03 PM (6 years, 9 months ago)

Thanks boogie!


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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


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InvisibleMoabfighter
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Registered: 12/13/15
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Re: Cultivation General Discussion [Re: amidogen]
    #24261744 - 04/21/17 12:44 PM (6 years, 9 months ago)

No one really likes to talk in cult potd you guys shoot the shit with me in this thread.



Not quite a wall, but I'll have my wall before Mr POTUS does.

Also, ken, look at the PE!!! Fuck yes.


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KSSS And PE WBS.
 


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OfflineCagney
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Registered: 09/12/16
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24261787 - 04/21/17 12:59 PM (6 years, 9 months ago)

Will one of you fine people post me a link on some LC recipes, I searched it and everything that came up was old as dirt. Also how to prepare the lids on the jars, I saw someone posted how they prepped the lids before but for the life of me can't find it. Fixing to start my first Liquid cultures any advice is appreciated. Thanks in advance.


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"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


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InvisibleChRnZN
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Registered: 12/21/08
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Re: Cultivation General Discussion [Re: Cagney]
    #24261795 - 04/21/17 01:01 PM (6 years, 9 months ago)



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Invisiblec10h12n2o
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Registered: 01/21/15
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Re: Cultivation General Discussion [Re: ChRnZN]
    #24261828 - 04/21/17 01:17 PM (6 years, 9 months ago)

Make sure you read up on the pros and cons of LC vs LI, and make sure that LC  actually appropriate for your needs

LCs only main benefit over liquid inoculant is the huge potential for culture expansion. But you don't need expansion if all you want is a good liquid inoculant. It's a matter of weighing  the additional risks against any potential gains

LI has far less risk of culturing contams


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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InvisibleMad Season
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Re: Cultivation General Discussion [Re: c10h12n2o] * 1
    #24261832 - 04/21/17 01:20 PM (6 years, 9 months ago)

LC especially requires some flawless techniques. 1 CFU of bacteria could seriously fuck up the whole thing. If you're confident in your tek, it's very simple. LI on the other hand is used to inoc within the session, 1 CFU of introduced bacteria goes in 1 jar only. Very nice


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Mad Season]
    #24261960 - 04/21/17 02:20 PM (6 years, 9 months ago)

Try LI with barely any water. Looking at a jar, I couldn't have used even 50ml, and I inoculated nine jars of oats. Two are bunk. Seven look pretty great minus my grain prep.


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KSSS And PE WBS.
 


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24262128 - 04/21/17 03:49 PM (6 years, 9 months ago)

Moab pe love fresh air use so give them more then ypu normally wpukd


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Boogieman47]
    #24262131 - 04/21/17 03:51 PM (6 years, 9 months ago)

Quote:

Boogieman47 said:
Moab pe love fresh air use so give them more then ypu normally wpukd





Got it. Going to remove the bottom strip of micropore tape since the pins are coming in good. Thanks.


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KSSS And PE WBS.
 


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InvisibleTheMadHatter420
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Registered: 10/12/16
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24263115 - 04/21/17 11:58 PM (6 years, 9 months ago)

So I got a strange and funny little tidbit that could turn into something cool. like 7-10 days ago I emptied some QT jars of bacterial oats into a bucket. They ended up setting in the basement until now. Well they some how went on to colonize more, into one solid ~2.5gal block. I dug a hole in the garden and dumped it in. Smelled strong of mushrooms with a bit of the bacteria smell. I crumbled it apart in the hole and covered with soil. This is in the end of my vegetable garden where I dumped 2 pickup truck loads of horse and cow manure a year ago. We will see if anything comes of it. There was NO visible mold, yet the bucket sat open in my basement the whole time. I had honestly believed they were to bacterial to do anything with, then I saw they were still chugging along. Said to myself well fuck I just might get me some outdoor cubes, only time will tell.


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JOIN THE POW WOW


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OfflineManifoldPrime
Per Ardua Ad Astra
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Registered: 03/16/17
Posts: 1,313
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24263290 - 04/22/17 03:00 AM (6 years, 9 months ago)

I got my first pins lads!

Albeit in a PF jar that isnt fully colonised, so its probably a sign of stress. But I'm one step closer! I can clone the 2 to 2 plates when I make a batch of agar. Or just eat them since I dont have enough materials for agar right now.


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OfflineCagney
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Re: Cultivation General Discussion [Re: ManifoldPrime]
    #24263438 - 04/22/17 06:39 AM (6 years, 9 months ago)

Quick question fellas, I am fixing to inoculate some grain jars with wedges of agar. Is it best to use the dishes with the rhizo* or the fluffy kind, totome* sorry can't remember the names of it.

And as for my earlier post about LC recipes I am basically just wanting to use it for inoculation. I figured liquid would be quicker to colonize my grain than using wedges of agar.


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"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


Edited by Cagney (04/22/17 07:39 AM)


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OfflineManifoldPrime
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Re: Cultivation General Discussion [Re: Cagney]
    #24263554 - 04/22/17 08:26 AM (6 years, 9 months ago)

Rhizo is generally considered more aggressive so use that.


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |


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Offlinedhype773
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Re: Cultivation General Discussion [Re: ManifoldPrime]
    #24263616 - 04/22/17 09:12 AM (6 years, 9 months ago)

Ahhh, I've got nothing but my first cakes on my mind!  Today is only the fifth day since I inoculated them,  so no growth yet.  Happy that I don't see anything else in any of them either! I literally had a dream about them last night.  I never had this sort of anticipation with oysters lol. It would probably move faster if I could get the temp up closer to 80, but that would cost more, and the wife would kill me.  69/70 it is...


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InvisibleChanga Alchemist
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Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
Re: Cultivation General Discussion [Re: dhype773]
    #24263625 - 04/22/17 09:18 AM (6 years, 9 months ago)

69/70 is better than 80. They're going to build internal heat as they grow, too hot will lead to a bacterial breeding ground. Especially if you're using a spore solution.


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Offlinedhype773
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Re: Cultivation General Discussion [Re: Changa Alchemist]
    #24263635 - 04/22/17 09:25 AM (6 years, 9 months ago)

Quote:

Shomann said:
69/70 is better than 80. They're going to build internal heat as they grow, too hot will lead to a bacterial breeding ground. Especially if you're using a spore solution.



Yup, MS syringe.  I can't wait to see some white though!  I'm like a kid on Christmas.  Only problem is my job just rescheduled my vacation to June 4th.  So now I'm going to have to stall the jars I guess, if I can't fruit in time. I can throw them in the fridge once they are colonized right?


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InvisiblemushboyMDiscord
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Registered: 04/24/05
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24263649 - 04/22/17 09:38 AM (6 years, 9 months ago)

Quote:

TheMadHatter420 said:
So I got a strange and funny little tidbit that could turn into something cool.




you made an outdoor patch. kinda how you make one. post pics when/if it fruits. outdoor shrooms are awesome. so mystical:stoned:


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OfflineCrispykoot
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Re: Cultivation General Discussion [Re: ManifoldPrime]
    #24263926 - 04/22/17 12:07 PM (6 years, 9 months ago)

Quote:

ManifoldPrime said:
Rhizo is generally considered more aggressive so use that.





Rhizo growth means very little in relation to fruiting ability...Somebody else with more experience can explain it better, but don't be afraid of tomentose growth.


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:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:


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OfflineManifoldPrime
Per Ardua Ad Astra
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Registered: 03/16/17
Posts: 1,313
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Re: Cultivation General Discussion [Re: dhype773]
    #24264032 - 04/22/17 12:46 PM (6 years, 9 months ago)

We'll some consolidation time is fine but the fridge will keep them in stasis for a while until they fruit. I got some plates going in if I cant get more agar petris in time.


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Crispykoot]
    #24264119 - 04/22/17 01:24 PM (6 years, 9 months ago)

Quote:

Crispykoot said:
Quote:

ManifoldPrime said:
Rhizo is generally considered more aggressive so use that.





Rhizo growth means very little in relation to fruiting ability...Somebody else with more experience can explain it better, but don't be afraid of tomentose growth.



Rhizo is better to see the actual growth, easier to see if it has bacteria and happens with lower nutes its supposed starve the myc so it grows faster looking for nutrients but ya tomentose is just as good you can take a nice rhizo growing plate and up the nutes and after a few transfers you will see it gping back to tomentose.... tomatoe tomato


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