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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: van hatton]
    #24240252 - 04/13/17 11:53 AM (6 years, 9 months ago)

Was kinda looking around to see what you're into MadHat..

Quote:

TheMadHatter420 said:
So 1st my WALL~O~CAKES! lol


Then we got my supper mini-mono. This is one PINT purple mystic cake crumbled into one quart of CVG. It has been 8 days since I spawned it. Has been at full colonization for 2 days. Great growth so far.



Then we have 3 grain jars that I started with just a COUPLE drops of spore solution each. Saw good growth and shook a few days ago, rebounded VERY well. 2 Purple mystic and one Z strain. Will be mini-mono soon in the hunt for good clones.







Look like you have PF down. Which is great. I had it down and was doing nice cakes to bulk. Switched to WBS last year and had zero luck, almost zero shrooms, now trying oats and think I may have it.

Anyway, I wouldn't keep wasting time with spores to oats. Spores to PF Tek ok, Spores to anything else without using agar to clean up pretty much big waste of time and if you get it to work, I wouldn't call it luck, but it isn't working because you got it down good.

IMO I'd either stick to PF Tek, or focus really really hard on bulk and tell yourself the first few times may likely not work out. It isn't PF Tek. It's a lot fucking tougher than PF Tek. Anyone can stab a needle in a half pint and squirt liquid. Grains are a challenge, and if you're the type that likes to run through a video game without ever dying, id keep doing PF Tek.

Keep it simple, it's easy to cross confuse outdoor info, PF info, WBS info etc together when you're new. Focus on the basics. Focus on bods Oat prep Tek, it's a good one.


--------------------
KSSS And PE WBS.
 


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InvisibleTheMadHatter420
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Registered: 10/12/16
Posts: 12,941
Re: Cultivation General Discussion [Re: Moabfighter]
    #24240665 - 04/13/17 02:52 PM (6 years, 9 months ago)

Yea, I think PF tek is easy. Only lost 1 out of 34 pint to mold. Considering I lifted lids and put a few drops on top, well that's not bad. I to am working with oats, and AGAR. So the metal banded Pints were some pore solution I made up and used the next day, to limit bacteria growth. The white lids, with the exception of the 1 MS AA+ are from LC I had. I did a couple jars with ONLY a few drops of spore to grains just fro the fuck of it. They are intended for hunting of a clone. Got a little bacteria in there but doing better than those fucking Costa Rica. I will be using 4 pints to 4 QT CVG for those cakes in mini-monos. I do this while I try to get clean cultures for grains. This gives me 2 ways to fill my mono's, one that's like 98% successful, and then one that I struggle with.


--------------------
JOIN THE POW WOW


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OfflinePinPornProducer
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24240883 - 04/13/17 04:19 PM (6 years, 9 months ago)

Gonna pick up some king oysters, blue oysters, shiitake, and reishi. Several questions...

1- when ordering lcs, are they clean enough to expand to more lc?

2- As far as agar goes, is normal pda/mea good enough for these?

3- spawn/sub materials. What's needed?

4- Shiitake and reishi can be grown in monos correct? But oysters need to be grown in log style bags, how about King oysters?

I'm probably just going to have to turn my grow room into a greenhouse anyways but just wanted to know a few things. Thanks guys :takingnotes:


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Invisiblevan hattonFacebookDiscord
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Re: Cultivation General Discussion [Re: PinPornProducer]
    #24240943 - 04/13/17 04:40 PM (6 years, 9 months ago)

1. Put to agar first then decide.

2. Yes

3. Straw wood chips sawdust/fuel pellets bran

4. Someone's actually doing kings in a mono on edible pic of the day thread right meow your best bet is straw logs.

Check out mushfarmer on YouTube he's got alot of good info easier then looking for the info. I'm going to expand the shit out of my kings here soon

Hope that helps.

I have my own question what moisture content for grains do you guys strive for?


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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OfflinePinPornProducer
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Re: Cultivation General Discussion [Re: van hatton]
    #24240977 - 04/13/17 04:55 PM (6 years, 9 months ago)

Thanks for the info man

I guage texture by chewing a few every few minutes until I got em where I want em. Hard to describe but kind of firm but chewy, a little pressure to squeeze and crush them.


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OfflineCrispykoot
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Re: Cultivation General Discussion [Re: PinPornProducer]
    #24241018 - 04/13/17 05:08 PM (6 years, 9 months ago)

Quote:

PinPornProducer said:
Gonna pick up some king oysters, blue oysters, shiitake, and reishi. Several questions...

1- when ordering lcs, are they clean enough to expand to more lc?

2- As far as agar goes, is normal pda/mea good enough for these?

3- spawn/sub materials. What's needed?

4- Shiitake and reishi can be grown in monos correct? But oysters need to be grown in log style bags, how about King oysters?

I'm probably just going to have to turn my grow room into a greenhouse anyways but just wanted to know a few things. Thanks guys :takingnotes:




MEA is fine...Some of my master slants come on MEP.

Spawn on rye or millet is typical then to sawdust if thats your thing.

Oysters do really well on Spelt or Wheat hulls...no need to chop them and they come clean. Lower the spawn rates to 10-20% max..

I use produce bags to fruit mine with polyfill as a ge filter. I use a 55 gallon drum, a giant burlap bag to fit and a "tiger torch" to heat...Proper pasteurization. Oysters will fruit out of the side of monos into a humid room....

Shiitake: chips/bran/sawdust or you can run them on hardwood fuel pellets.

PM if you have questions...This is my 4th season running commercial edibles.


--------------------






:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:


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OfflinePinPornProducer
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Re: Cultivation General Discussion [Re: Crispykoot]
    #24241022 - 04/13/17 05:10 PM (6 years, 9 months ago)

Will do man, appreciate it :thumbup: thanks


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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: PinPornProducer]
    #24241028 - 04/13/17 05:13 PM (6 years, 9 months ago)

Quote:

PinPornProducer said:
Shiitake and reishi can be grown in monos correct?



You should grow your reishi in bags. Saves tons of space and you can just leave them in the
bags till harvest

Those are all currently under my work desk. 18 bags total, 3lb sawdust, 1/2 quart of spawn.
I used a little too much water but they still colonized and are fruiting well enough


--------------------


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: natedawgnow]
    #24241064 - 04/13/17 05:24 PM (6 years, 9 months ago)

:takingnotes:

nice info. thanks for being shroomy. i just ordered a portabello and shiitake lcs:awesanta:


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: mushboy]
    #24241088 - 04/13/17 05:34 PM (6 years, 9 months ago)

Quote:

mushboy said:
:takingnotes:

nice info. thanks for being shroomy. i just ordered a portabello and shiitake lcs:awesanta:





No shit? I wanna grow portobellos too


--------------------
KSSS And PE WBS.
 


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OfflineThedillestpickle
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24241298 - 04/13/17 06:59 PM (6 years, 9 months ago)

I'm doing a side by side experiment:

1 tub with 100% pure vermiculite

1 tub with 100% pure coir(well cooked)

Both will be sterilized using hot water, both receiving 10 jars made from same LC, really clean amazonian. 

Same volume of water will go to both tubs, and I will try my best to hit similar field capacity on both tubs

Here's my ratios for sub to water for each tub

Coir:  2.16kg coir to 12L water

Verm:  27L verm to 12L water

Each tub is 105qt sterilite


~Hopefully that gets me close to field capacity for both substrates


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Thedillestpickle]
    #24241346 - 04/13/17 07:13 PM (6 years, 9 months ago)

Quote:

Thedillestpickle said:
I'm doing a side by side experiment:

1 tub with 100% pure vermiculite

1 tub with 100% pure coir(well cooked)

Both will be sterilized using hot water, both receiving 10 jars made from same LC, really clean amazonian. 

Same volume of water will go to both tubs, and I will try my best to hit similar field capacity on both tubs

Here's my ratios for sub to water for each tub

Coir:  2.16kg coir to 12L water

Verm:  27L verm to 12L water

Each tub is 105qt sterilite



Neat man keep us posted.
~Hopefully that gets me close to field capacity for both substrates




--------------------
KSSS And PE WBS.
 


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Offlinezajcob
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24241433 - 04/13/17 07:40 PM (6 years, 9 months ago)

I've read a few threads regarding cutting agar wedges for grain inoculation.  A question that still remains is whether people cut around the little agar wedge in the center of the petri from previous transferring or just cut right through it.  Getting a sizeable wedge and the whole process in general would be easier if I was to just cut right through it.  The reason I might think to avoid it is that it was involved in a transfer process while mycelium that has grown out from the previous little wedge in the center wasn't exposed in the same fashion to air in the SAB.


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InvisibleChanga Alchemist
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Re: Cultivation General Discussion [Re: zajcob]
    #24241486 - 04/13/17 08:01 PM (6 years, 9 months ago)

We cut from the leading edge of the petri for transfers. If those transfers are clean we keep them and use them. If they aren't clean, like what your referring to, and they're clearly not clean... We don't use it.


--------------------
:ganja::mushroom::mushroom::flyhigh:
:sporedrop:~~~:peyote::sanpedro:~~~:sporedrop:

:chugbeer:


Edited by Shomann (04/13/17 08:02 PM)


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Offlinezajcob
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Re: Cultivation General Discussion [Re: Changa Alchemist]
    #24241603 - 04/13/17 08:41 PM (6 years, 9 months ago)

If I understand you correctly, it seems that if you wanted to inoculate two grain jars with one petri of colonized agar culture for example, you would simply cut it in half right through the center if the plate as a whole appeared to be clean.  But people say that the edges of a plate can harbor contamination, so with respect to that maybe a square cut around and through the center of the circular mycelium culture would be better to get two wedges that didn't include the edge of the agar disc.


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Invisiblevan hattonFacebookDiscord
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Re: Cultivation General Discussion [Re: zajcob]
    #24241624 - 04/13/17 08:47 PM (6 years, 9 months ago)

No the edges don't harbor contams as you think they can be there but with good eyes easy to spot and that's usually referring to bacteria not mold.

Generally bacteria can't spread around a plate without water rolling around inside. Mold will take over the entire thing.

Look up the tiger drop. You use an entire pasty plate edges bottom the whole thing .

To answer your above question if the transfer you just took to a plate is contaminated you will know quickly.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisibleJust_A_Noob
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Re: Cultivation General Discussion [Re: van hatton]
    #24241665 - 04/13/17 08:59 PM (6 years, 9 months ago)



How bout this one guys?  This is a PE clone almost ready.  It seems to be having a hard time covering the top.  I shook at 30%.  Is it actually colonized at the top or does it need more time?


--------------------

Wearing a mask is bad for my physical, emotional, and spiritual health.
Complying = Consent
Wide Mouth 1/2 Pint No-Pour TEK
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Offlinezajcob
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Re: Cultivation General Discussion [Re: van hatton]
    #24241688 - 04/13/17 09:06 PM (6 years, 9 months ago)

The reason I wonder about the edge of a plate is that it is advised not to let cultures grow out all the way to the edge.  But maybe that is because the mycelium will even try to climb up the side beyond that.  I actually used the tiger drop from ten pasties to successfully colonize 10 WBS quarts just recently and I'm eager to spawn a couple of monos with them, and I did note that the edges necessarily were included, although a pasty is quite a bit deeper than a petri to be sure.

So to be perfectly clear regarding my initial question though, you have no qualms with cutting right through the center of a petri culture to obtain a wedge for grain inoculation?


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OfflineThedillestpickle
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Re: Cultivation General Discussion [Re: zajcob]
    #24241713 - 04/13/17 09:12 PM (6 years, 9 months ago)

Quote:

zajcob said:
The reason I wonder about the edge of a plate is that it is advised not to let cultures grow out all the way to the edge.  But maybe that is because the mycelium will even try to climb up the side beyond that.  I actually used the tiger drop from ten pasties to successfully colonize 10 WBS quarts just recently and I'm eager to spawn a couple of monos with them, and I did note that the edges necessarily were included, although a pasty is quite a bit deeper than a petri to be sure.

So to be perfectly clear regarding my initial question though, you have no qualms with cutting right through the center of a petri culture to obtain a wedge for grain inoculation?




Cutting though the center is fine if you know you took a clean transfer from a clean plate, and you observed the plate at is grew out and can see that no bacteria was present.  It's still better to leave the center alone just in case there was bacteria, but I think you would be able to see that. 

I would avoid the edge of the plate, especially if there is any visible condensation.  Just transfer what you can see is clean.


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Invisiblevan hattonFacebookDiscord
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Re: Cultivation General Discussion [Re: Thedillestpickle]
    #24241747 - 04/13/17 09:20 PM (6 years, 9 months ago)

:whathesaid: just monitor the plate for the first few days if taking from a contams plate.

Bacteria usually shows 24-48 hours and mold 48+.

For Petris (I've never used a pasty plate) I cut it into 4 pieces just an x once fully colonized and drop it between 2 jars 2 wedges per jar.

This is honestly overkill. I see alot of people use a full plate for 4 jars. I just like the week colonization time once leap off.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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