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Saxifrage
perennial herb



Registered: 08/12/11
Posts: 361
Loc: on a rock
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Re: Cultivation General Discussion [Re: QuantumZ00] 1
#29072096 - 12/30/24 08:14 AM (16 days, 18 hours ago) |
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People that post bad advice usually end up with some bad ratings pretty quick around here. You know there's people that would troll the shit out of this forum specifically just to get that tag too...
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QuantumZ00
Endospore Explorer



Registered: 07/11/24
Posts: 3,203
Loc: Pennsylvania in Appalachia
Last seen: 6 hours, 41 minutes
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Re: Cultivation General Discussion [Re: Saxifrage]
#29072098 - 12/30/24 08:17 AM (16 days, 18 hours ago) |
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See that's why i don't come up with ideas when stoned because people would totally troll to get that tag.
I always forget about the ratings thing because I never use it really, never to negatively rate someone anyway haha
-------------------- Endlessly endorsing empathy empirically energizing existence™
~Always practice radical kindness~
R.I.P. Betty Boop, the Greatest Mother who ever lived. October 1961-November 2024.
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Mr Piggy
Playing "queer pass"



Registered: 09/29/11
Posts: 11,957
Loc: bioilliminicent©️
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Re: Cultivation General Discussion [Re: QuantumZ00] 5
#29072119 - 12/30/24 08:49 AM (16 days, 18 hours ago) |
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Who said the TC tag isn’t a dunce cap?
-------------------- 🅃🄴🄰🄼 🄵🄾🄸🄻
New to cultivation or the shroomery? Observe the hitchhiker's guide
The Hitchhiker's Guide to the Shroomery
Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."
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Scemilope
Jar Washing Enthusiast


Registered: 11/16/22
Posts: 1,428
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Re: Cultivation General Discussion [Re: Mr Piggy] 2
#29072135 - 12/30/24 09:05 AM (16 days, 17 hours ago) |
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Quote:
Mr Piggy said: Who said the TC tag isn’t a dunce cap?

-------------------- This hobby has taught me many things.
Most of those things are patience.
The Hitchhiker's Guide to the Shroomery
LAGM 2025
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HILLBILLY OUTLAW
Above And Beyond!



Registered: 04/21/22
Posts: 4,066
Loc: Luckenbach Texas
Last seen: 6 hours, 8 minutes
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Re: Cultivation General Discussion [Re: Mr Piggy] 3
#29072163 - 12/30/24 09:40 AM (16 days, 17 hours ago) |
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Quote:
Mr Piggy said: Who said the TC tag isn’t a dunce cap?


--------------------

🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.
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LewDoja
mad $cientist, ganjacologist


Registered: 09/18/09
Posts: 2,889
Loc: A fictional tale, in fictional...
Last seen: 7 hours, 58 minutes
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
Posts: 8,259
Loc: Canada
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Re: Cultivation General Discussion [Re: Mr Piggy]
#29072452 - 12/30/24 03:13 PM (16 days, 11 hours ago) |
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 15,101
Loc: Hole
Last seen: 4 hours, 1 minute
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Re: Cultivation General Discussion [Re: Stipe-n Cap] 1
#29072454 - 12/30/24 03:15 PM (16 days, 11 hours ago) |
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Wait wait wait wait
WHY would be think that. That’s ass backwards and retarded
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Mr Piggy
Playing "queer pass"



Registered: 09/29/11
Posts: 11,957
Loc: bioilliminicent©️
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Re: Cultivation General Discussion [Re: Smellyhobbit]
#29072459 - 12/30/24 03:24 PM (16 days, 11 hours ago) |
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I mean, I'm literally retarded and I know not to do that
-------------------- 🅃🄴🄰🄼 🄵🄾🄸🄻
New to cultivation or the shroomery? Observe the hitchhiker's guide
The Hitchhiker's Guide to the Shroomery
Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."
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LewDoja
mad $cientist, ganjacologist


Registered: 09/18/09
Posts: 2,889
Loc: A fictional tale, in fictional...
Last seen: 7 hours, 58 minutes
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Re: Cultivation General Discussion [Re: Mr Piggy]
#29072494 - 12/30/24 04:06 PM (16 days, 10 hours ago) |
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The logic behind it (not to condone it) is that morel myc is super fast. But the microbial load is too high for the Myc to recover before being overwhelmed on agar.
So with continuous stirring the lc allows the myc (along with everything else) to recover & then being cleaned up on agar .
Honey also has VERY mild antimicrobial properties....that maybe 🤔, if the stars were aligned just right, had minimal effectiveness.
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
Posts: 8,259
Loc: Canada
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Re: Cultivation General Discussion [Re: LewDoja] 1
#29072501 - 12/30/24 04:20 PM (16 days, 10 hours ago) |
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Full strength honey, maybe, but not diluted and then exposed to every known environmental contaminant along the side of a road. The effect would be zero.
This is why water agar is used for wild clones.
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LewDoja
mad $cientist, ganjacologist


Registered: 09/18/09
Posts: 2,889
Loc: A fictional tale, in fictional...
Last seen: 7 hours, 58 minutes
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#29072753 - 12/30/24 08:53 PM (16 days, 6 hours ago) |
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word!
Was just trying to come up with something anything, that could possibly be used to their reasoning... stretching out the imagination muscles.
Do you pre-treat wild specimens before plating? (H2O2 // bleach solution)
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Churpee Choozle
Towel



Registered: 11/11/24
Posts: 305
Loc: NW Europe
Last seen: 28 minutes, 6 seconds
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Re: Cultivation General Discussion [Re: LewDoja] 1
#29072934 - 12/31/24 02:56 AM (16 days, 7 minutes ago) |
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Water agar, select myc away from original tissue, introduce to more nutes. I have a nice bottle of hydrogen peroxide, but it invariably gives me more problems than solutions, guess I never got my dilution right. I was also thinking about what he could possibly mean by too dirty for agar. Was thinking that maybe in plain water at a certain (low) temp the myc has certain advantages... But then why lc...
-------------------- "Shoot! Fuck! Scag! Blood! Heroin! Rape! Cheap! Communist! Jab it right into your fucking eyeballs!"
- Hunter S. Thompson
Touching your first grows unnecessarily is very similar to crack.
- Alaskappalachian
Things to consider when you (re-) start growing mushrooms. https://www.shroomery.org/forums/showflat.php/Number/28501530/fpart/1/vc/1
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3.A.M
Boop!



Registered: 10/17/22
Posts: 2,090
Loc: Oz
Last seen: 2 hours, 33 minutes
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Re: Cultivation General Discussion [Re: LewDoja] 3
#29073006 - 12/31/24 06:29 AM (15 days, 20 hours ago) |
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Best method I’ve got for wild specimens and in fact all ms grows now is gently swab between a set of gills rotating the swab the entire length, package that swab then gently swab with a fresh one in the exact same spot and same method then using that second one to streak, theory is you pick up the majority of contam on the first pass leaving it a little cleaner for the second, I get 100% clean success rate with my tub grows this way and the wild grows vary but if I keep track of the plate I’ll absolutely get a heap of transfers before bacteria takes over, if it does at all. Worst case you can streak again a couple weeks down the track with either of those swabs after they’ve dried out and the contam rate would’ve dropped even further. Peroxide is great as a buffer but I now mainly use it for prepping wild clones and cleaning certain utensils.
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Fiet
Stranger

Registered: 01/29/23
Posts: 463
Last seen: 21 minutes, 47 seconds
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Re: Cultivation General Discussion [Re: 3.A.M] 1
#29073181 - 12/31/24 10:31 AM (15 days, 16 hours ago) |
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After i add more water the Growth looking way better what you think?
P. Natalensis Strain
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A.k.a
Stranger



Registered: 10/27/19
Posts: 17,846
Loc: Gaming the system
Last seen: 6 hours, 15 minutes
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Re: Cultivation General Discussion [Re: Fiet] 1
#29073210 - 12/31/24 11:09 AM (15 days, 15 hours ago) |
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Shouldn’t you be able to get clean tissue from inside a wild mushroom same as an indoor one??
They can’t be so riddled with bugs that the whole thing is dirty right
--------------------
LAGM2020     
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KlarnEvil9
Spoot the juice!!!



Registered: 06/13/24
Posts: 535
Loc: Upper Earth
Last seen: 7 hours, 15 minutes
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Re: Cultivation General Discussion [Re: A.k.a]
#29073222 - 12/31/24 11:25 AM (15 days, 15 hours ago) |
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IME - The ones I have tried were all filthy. Like a fly larva crawling around on the plate dirty. Most also turned pinkish-orange within 36-48 hours, some nasty looking shit on those. None of the wild fruit contams looked anything like my other contams.
All were inner stipe tissue taken in the SAB and put to agar.
-------------------- I am Klarn of the Cosmos, ninth of his name! Keeper of the green plates, protector of arachnids!
(and killer of threads!)
Turns out that I'm an amateur at everything...
Read more, post less! LAGM 2025
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
Posts: 8,259
Loc: Canada
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Re: Cultivation General Discussion [Re: A.k.a] 1
#29073235 - 12/31/24 11:41 AM (15 days, 15 hours ago) |
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Quote:
A.k.a said: Shouldn’t you be able to get clean tissue from inside a wild mushroom same as an indoor one??
They can’t be so riddled with bugs that the whole thing is dirty right
Morels are hollow tubes, so while waving it around for the camera on a dirt road while on a quad, I'd wager not. I've pulled apart plenty of bears tooth, chanty, etc, and generally end up with a disgusting mess on agar.
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3.A.M
Boop!



Registered: 10/17/22
Posts: 2,090
Loc: Oz
Last seen: 2 hours, 33 minutes
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Re: Cultivation General Discussion [Re: A.k.a]
#29073445 - 12/31/24 02:22 PM (15 days, 12 hours ago) |
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Quote:
A.k.a said: Shouldn’t you be able to get clean tissue from inside a wild mushroom same as an indoor one??
They can’t be so riddled with bugs that the whole thing is dirty right
I always clone and streak but yes, clean on the inside, the peroxide is to minimise chances of contams getting blown or bumped from the outside onto clean areas, with pans it’s especially needed but I don’t really get bugs etc at all, I’m sure they’ve crawled over them but they’re rarely present when I pick them.
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smalltalk_canceled
Babnik



Registered: 07/13/20
Posts: 3,174
Last seen: 14 hours, 2 minutes
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Re: Cultivation General Discussion [Re: 3.A.M] 2
#29073684 - 12/31/24 07:10 PM (15 days, 7 hours ago) |
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Im so retarded. Please agree, its the truth
I sent my semilanceata samples to ohio
Taking Christmas break 1 of desember and still chilling yet
When i should have sent it to California Where Alan actually is (...)
Im a idiot
The biggest moron of them all
Happy new Years Shroomery, thank you still for it all
You perfect
-------------------- Willpower is the one true virtue
  
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