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OnlineSmellyhobbit
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Re: Cultivation General Discussion [Re: TheOffice] * 4
    #28574426 - 12/08/23 01:39 PM (1 month, 19 days ago)

I doubt that’s psilocybin in those drops


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Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: Cultivation General Discussion [Re: Smellyhobbit] * 1
    #28574431 - 12/08/23 01:44 PM (1 month, 19 days ago)

Quote:

Smellyhobbit said:
I doubt that’s psilocybin in those drops



Yeah that's the risk of anything processed - and what a good reagent test kit helps with.


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OfflineYoshiTrainer
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Re: Cultivation General Discussion [Re: wavyedge]
    #28574452 - 12/08/23 01:56 PM (1 month, 19 days ago)

I'll have to find it but one process to isolate psilocybin uses glacial acetic acid then I believe methylchloride?


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Invisiblestockw

Registered: 08/31/21
Posts: 450
Re: Cultivation General Discussion [Re: TheOffice]
    #28574456 - 12/08/23 01:59 PM (1 month, 19 days ago)

Yeah, they're incredible. It's my main motivation to understand the process more. Me & my housemates at the time would buy a bottle once a month or more depending on how much we were partying. But this guy sold a 50ml dropper bottle for 100 quid. 3 drops would get you nice, 6 would get you flying. Overall it was an amazing product and I regret not asking him more questions. I strive to recreate a product of similar quality.

(It was definitely psilocybin)


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Invisiblethirdeyewild
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Re: Cultivation General Discussion [Re: stockw]
    #28574463 - 12/08/23 02:03 PM (1 month, 19 days ago)

Never heard of this but I'm intrigued. The biggest barrier to tripping for me is the thought of chocking back the tea or fresh shrooms. I should probably try gummies one of these days.


--------------------


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OfflineYoshiTrainer
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Re: Cultivation General Discussion [Re: stockw] * 3
    #28574467 - 12/08/23 02:06 PM (1 month, 19 days ago)

Gummies are awesome 3rdEye!

Here is the extraction article.

From

https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/chem.201904363



"Natural product extraction. Initially, mycelia and carpophores were
lyophilized, ground, and extracted with anhydrous MeOH, as described to
extract 1 gently and to minimize its artificial dephosphorylation to 2.
[4c]
For improved carboline yields, the fungal biomasses (mycelia,
carpophores, or sclerotia) were lyophilized, pulverized, and the powder
solved in 0.1 M HCl and subsequently extracted with methylene chloride
(1:1, v/v). The aqueous phases were collected, the pH value adjusted to
12 using NaOH, which was followed by extractions with methylene
chloride. The organic phases were dried under reduced pressure in a
rotary evaporator. The resulting crude extracts were dissolved in
methanol, centrifuged and filtered, and subsequently used for
chromatographic analysis or purification. To quantify 4 titers in fungal
biomass, the areas under the curve (AUCs) in the extracted ion
chromatograms were determined and referenced to a standard curve
recorded with authentic 4.
Chromatographic purification of 4 and 5. Preparative HPLC was
performed using an Agilent 1260 instrument equipped with Phenomenex
Luna C18 column (250 × 21.2 mm, 10 µm particle size), and run with
0.1% trifluoroacetic acid (TFA) in water (solvent A) and acetonitrile (ACN,
solvent B). The flow was 20 mL min–1. A linear gradient was applied with
an increase from 10 to 100% B within 20 min. β-carbolines were further
purified by semipreparative HPLC using an Agilent 1200 instrument
equipped with a Zorbax Eclipse XDB-C18 column (250 × 9.4 mm, 5 µm).
and the same solvents, applying a flow of 2 mL min–1 and a linear
gradient from 10 to 100% B within 10 min. The final purification was
accomplished with the same solvents and instrument, but using a
Phenomenex Synergi RP-80 column (250 × 10 mm, 4 µm) and a gradient
that included an initial hold at 30% B for 1 min, an increase to 65% B
within 10 min, and to 100% B within further 30 sec. This procedure
yielded 3.5 mg of 4 and 14.4 mg of 5, which were dissolved in DMSO-d6
for subsequent NMR analysis (below).
HPLC and mass spectrometry. HPLC and mass spectrometry were
performed on a Thermo Accela liquid chromatograph equipped with a C18
column (Grom-Sil 100 ODS-0 AB, 250 × 4.6 mm, 3 µm) fitted to an
Exactive Orbitrap spectrometer, using electrospray ionization. The
respective diode array detectors covered the wavelength range of
λ=200–400 nm. Initially, HPLC-UV chromatograms were extracted at
λ=280 nm (to detect 1), later at λ=300 nm to detect β-carbolines.
Conditions for HPLC included solvents 0.1% TFA in water (A) and 0.1%
TFA in ACN (B) at a flow rate of 0.4 mL min–1. The gradient was: initial
hold at 10% B for 1 min, and linear increase to 98% B within 4 min.
Standard analytical runs were performed on a Thermo Vanquish Horizon
UHPLC system equipped with a diode array and a fluorescence detector.
This instrument was equipped with a Phenomenex Kinetex XB-C18
column (100 × 2.1 mm, 1.7 µm particle size). For fluorescence detection,
excitation and emission were at λ=340 and 410 nm, respectively.
Solvents were 0.1% formic acid (FA) in water (A) and ACN (B) at a flow
rate of 1 mL min–1. The gradient was: initial hold at 5% B for 1 min, and
linear increase to 100% B within 15 min. Chromatography and mass
spectrometry to quantify the concentration of 4 was done on an Agilent
1290 Infinity II UHPLC instrument with a diode array detector (DAD) and
interfaced to a 6130 quadrupole mass detector, run in ESI mode. The
chromatograph was equipped with a Phenomenex Luna Omega Polar
C18 50 × 2.1 mm (1.6 µm particle size) and a guard column. Separation
was at 25°C and a flow of 0.5 mL min-1. Mobile phase A was 0.1%
aqueous FA, phase B was ACN + 0.1% FA. A linear gradient was applied
(% B): initially 1%, within 3 min to 10%, and within further 1 min to 100%.
UV/Vis spectra were recorded with the diode-array detector during LCMS
analyses. Samples were dissolved in MeOH.
MALDI-MS imaging. P. cubensis mycelium was directly"


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OfflineYoshiTrainer
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Re: Cultivation General Discussion [Re: YoshiTrainer]
    #28574471 - 12/08/23 02:10 PM (1 month, 19 days ago)

StockW, you can make some pretty strong extracts, depending on starting amount of mush and how much you reduce the extraction later. Did the drops taste like anything? Heavily concentrated mushroom extract can taste nasty!


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: Cultivation General Discussion [Re: YoshiTrainer] * 1
    #28574479 - 12/08/23 02:21 PM (1 month, 19 days ago)

DCM is nasty stuff, probably carcinogenic. For DMT and mescaline, extraction is really a necessity IMO, but from what procedures I've seen I'm not confident about extracting psilocybin, and the mushrooms are already so potent. :shrug:


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OfflineTheOffice
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Registered: 05/13/21
Posts: 83
Loc: The Netherlands
Last seen: 3 hours, 7 minutes
Re: Cultivation General Discussion [Re: Smellyhobbit]
    #28574488 - 12/08/23 02:24 PM (1 month, 19 days ago)

Quote:

Smellyhobbit said:
I doubt that’s psilocybin in those drops





I also have my doubts,

But as Stockw said, there are several drops sold as psilocybin drops.

What would be your argument(s) for your doubt(s)?


--------------------
Cum grege non gradior







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OfflineTheOffice
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Registered: 05/13/21
Posts: 83
Loc: The Netherlands
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Re: Cultivation General Discussion [Re: YoshiTrainer]
    #28574495 - 12/08/23 02:28 PM (1 month, 19 days ago)

Quote:

YoshiTrainer said:
Gummies are awesome 3rdEye!

Here is the extraction article.

From

https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/chem.201904363



"Natural product extraction. Initially, mycelia and carpophores were
lyophilized, ground, and extracted with anhydrous MeOH, as described to
extract 1 gently and to minimize its artificial dephosphorylation to 2.
[4c]
For improved carboline yields, the fungal biomasses (mycelia,
carpophores, or sclerotia) were lyophilized, pulverized, and the powder
solved in 0.1 M HCl and subsequently extracted with methylene chloride
(1:1, v/v). The aqueous phases were collected, the pH value adjusted to
12 using NaOH, which was followed by extractions with methylene
chloride. The organic phases were dried under reduced pressure in a
rotary evaporator. The resulting crude extracts were dissolved in
methanol, centrifuged and filtered, and subsequently used for
chromatographic analysis or purification. To quantify 4 titers in fungal
biomass, the areas under the curve (AUCs) in the extracted ion
chromatograms were determined and referenced to a standard curve
recorded with authentic 4.
Chromatographic purification of 4 and 5. Preparative HPLC was
performed using an Agilent 1260 instrument equipped with Phenomenex
Luna C18 column (250 × 21.2 mm, 10 µm particle size), and run with
0.1% trifluoroacetic acid (TFA) in water (solvent A) and acetonitrile (ACN,
solvent B). The flow was 20 mL min–1. A linear gradient was applied with
an increase from 10 to 100% B within 20 min. β-carbolines were further
purified by semipreparative HPLC using an Agilent 1200 instrument
equipped with a Zorbax Eclipse XDB-C18 column (250 × 9.4 mm, 5 µm).
and the same solvents, applying a flow of 2 mL min–1 and a linear
gradient from 10 to 100% B within 10 min. The final purification was
accomplished with the same solvents and instrument, but using a
Phenomenex Synergi RP-80 column (250 × 10 mm, 4 µm) and a gradient
that included an initial hold at 30% B for 1 min, an increase to 65% B
within 10 min, and to 100% B within further 30 sec. This procedure
yielded 3.5 mg of 4 and 14.4 mg of 5, which were dissolved in DMSO-d6
for subsequent NMR analysis (below).
HPLC and mass spectrometry. HPLC and mass spectrometry were
performed on a Thermo Accela liquid chromatograph equipped with a C18
column (Grom-Sil 100 ODS-0 AB, 250 × 4.6 mm, 3 µm) fitted to an
Exactive Orbitrap spectrometer, using electrospray ionization. The
respective diode array detectors covered the wavelength range of
λ=200–400 nm. Initially, HPLC-UV chromatograms were extracted at
λ=280 nm (to detect 1), later at λ=300 nm to detect β-carbolines.
Conditions for HPLC included solvents 0.1% TFA in water (A) and 0.1%
TFA in ACN (B) at a flow rate of 0.4 mL min–1. The gradient was: initial
hold at 10% B for 1 min, and linear increase to 98% B within 4 min.
Standard analytical runs were performed on a Thermo Vanquish Horizon
UHPLC system equipped with a diode array and a fluorescence detector.
This instrument was equipped with a Phenomenex Kinetex XB-C18
column (100 × 2.1 mm, 1.7 µm particle size). For fluorescence detection,
excitation and emission were at λ=340 and 410 nm, respectively.
Solvents were 0.1% formic acid (FA) in water (A) and ACN (B) at a flow
rate of 1 mL min–1. The gradient was: initial hold at 5% B for 1 min, and
linear increase to 100% B within 15 min. Chromatography and mass
spectrometry to quantify the concentration of 4 was done on an Agilent
1290 Infinity II UHPLC instrument with a diode array detector (DAD) and
interfaced to a 6130 quadrupole mass detector, run in ESI mode. The
chromatograph was equipped with a Phenomenex Luna Omega Polar
C18 50 × 2.1 mm (1.6 µm particle size) and a guard column. Separation
was at 25°C and a flow of 0.5 mL min-1. Mobile phase A was 0.1%
aqueous FA, phase B was ACN + 0.1% FA. A linear gradient was applied
(% B): initially 1%, within 3 min to 10%, and within further 1 min to 100%.
UV/Vis spectra were recorded with the diode-array detector during LCMS
analyses. Samples were dissolved in MeOH.
MALDI-MS imaging. P. cubensis mycelium was directly"





This is all about extraction and identification right?

Im not sure extraction is the problem but storing it and keeping it shelf stable. And I mean in drop bottles you can bring to a party and staying potent for some weeks/months. (Not like in a freezer at -80 °C or something)

P.s. I know for a fact some vendors also put 4-aco-dmt in the bottles.. to "supplement the psilocybin" could this be a stabilizing factor or is it just to make up for the degradation of psilocybin/psilocin?


--------------------
Cum grege non gradior







Edited by TheOffice (12/08/23 02:31 PM)


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OfflineYoshiTrainer
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Registered: 04/30/22
Posts: 1,202
Loc: Castles made of sand
Last seen: 7 hours, 4 minutes
Re: Cultivation General Discussion [Re: wavyedge] * 1
    #28574502 - 12/08/23 02:30 PM (1 month, 19 days ago)

I agree Wavy, DCM is nasty! For my purposes, citric acid + water works very well! If you want portability, make gummies or even chocolates, nice and easy!


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OfflineSan Pedro GirlS
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I'm a teapot User Gallery


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Registered: 07/17/12
Posts: 2,385
Loc: Fuck off pig!🐷
Last seen: 12 hours, 54 minutes
Re: Cultivation General Discussion [Re: YoshiTrainer] * 3
    #28574510 - 12/08/23 02:33 PM (1 month, 19 days ago)

Quote:

YoshiTrainer said:
I agree Wavy, DCM is nasty! For my purposes, citric acid + water works very well! If you want portability, make gummies or even chocolates, nice and easy!



I make spearmint tea with lemon and honey. It completely masks the taste.


--------------------


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Invisiblestockw

Registered: 08/31/21
Posts: 450
Re: Cultivation General Discussion [Re: YoshiTrainer] * 1
    #28574511 - 12/08/23 02:33 PM (1 month, 19 days ago)

Quote:

YoshiTrainer said:
StockW, you can make some pretty strong extracts, depending on starting amount of mush and how much you reduce the extraction later. Did the drops taste like anything? Heavily concentrated mushroom extract can taste nasty!




Yes Yoshi! Good to see ya. They tasted bitter, really bitter. Colour was dark & rich, slightly viscous.


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OfflineYoshiTrainer
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Posts: 1,202
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Re: Cultivation General Discussion [Re: stockw]
    #28574552 - 12/08/23 03:04 PM (1 month, 19 days ago)

Might have been the real deal!?


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OfflineNichrome
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Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 7 hours, 10 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: mrmazdarx9] * 4
    #28574635 - 12/08/23 04:21 PM (1 month, 19 days ago)

Quote:

mrmazdarx9 said:
No tens damn sexy monos worthy of a 10 make you poke the Poly out :rofl:





Trying to use a translator to ask questions in an English speaking forum.


I think what you are asking for is advice in going straight to fruiting conditions with a monotub. In that case, just do it. That's what everybody does.

Once you spawn (dump mycelium into a tub) it's proper practice to expose the tub to fresh air exchange and light cycles without delay.

Good luck with the language berrier and welcome to the Shroomery.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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InvisibleMr Piggy
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Registered: 09/29/11
Posts: 8,379
Re: Cultivation General Discussion [Re: Nichrome] * 1
    #28574659 - 12/08/23 04:39 PM (1 month, 19 days ago)

Quote:

Nichrome said:
Quote:

mrmazdarx9 said:
No tens damn sexy monos worthy of a 10 make you poke the Poly out :rofl:





Trying to use a translator to ask questions in an English speaking forum.


I think what you are asking for is advice in going straight to fruiting conditions with a monotub. In that case, just do it. That's what everybody does.

Once you spawn (dump mycelium into a tub) it's proper practice to expose the tub to fresh air exchange and light cycles without delay.

Good luck with the language berrier and welcome to the Shroomery.





This is from page one homie, seven years ago

:hahthatsrich:


--------------------
🅃🄴🄰🄼 🄵🄾🄸🄻


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InvisibleHILLBILLY OUTLAWS
Above And Beyond!
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Registered: 04/21/22
Posts: 2,852
Loc: Luckenbach Texas
Re: Cultivation General Discussion [Re: Inocuole] * 1
    #28574794 - 12/08/23 06:41 PM (1 month, 19 days ago)

He’s testing you and you passed.

:curbyourenthusiasm:


--------------------

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.


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OfflineNichrome
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Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 7 hours, 10 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: HILLBILLY OUTLAW] * 6
    #28575232 - 12/09/23 01:49 AM (1 month, 19 days ago)

Left the dude hangin' for 7 years.



--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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Offlinetryptkaloids
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Registered: 02/08/15
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Re: Cultivation General Discussion [Re: Nichrome] * 5
    #28575263 - 12/09/23 03:51 AM (1 month, 19 days ago)

Smokes, let's go
:mygoditsfullofstars::bumpthread:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineTiamo
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Re: Cultivation General Discussion [Re: San Pedro Girl] * 2
    #28575301 - 12/09/23 04:58 AM (1 month, 19 days ago)

Quote:

San Pedro Girl said:
People have been isolating psilocybin from shrooms since the 1950s, but you think it can’t be done now? Hot water extraction is a fine way to make a tea, but it’s nowhere near pure. Reduce and try to crystallize and see what kind of trash you get.





I haven't made myself clear, I do think psilocybin can be extracted from mushrooms. I have no doubt, I have seen it described. What I do doubt is:

(1) If we could isolate other alkaloids such as baeocystin or aeruginascin and if they are even active and/or can be differentiated (subjectively) from psilocybin? I have not seen any techniques to extract these or experiences with them.

(2) If a psilocybin extraction can be differentiated from a mushroom experience including other alkaloids

And then another point that I have is why would you bother with a full spectrum (so, with all alkaloids present) mushroom extraction vs a hot water extraction? Isn't a hot water extraction basically a full spectrum extraction? For where I am standing I have reduced hot water extractions down to 1g/mL or more, which is a crazy potent liquid. I do not see any point for stronger extractions for the vast majority (99.9%+) of users.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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