|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 14 seconds
|
Re: Cultivation General Discussion [Re: TheOffice] 4
#28574426 - 12/08/23 01:39 PM (1 month, 19 days ago) |
|
|
I doubt that’s psilocybin in those drops
|
wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
|
Re: Cultivation General Discussion [Re: Smellyhobbit] 1
#28574431 - 12/08/23 01:44 PM (1 month, 19 days ago) |
|
|
Quote:
Smellyhobbit said: I doubt that’s psilocybin in those drops
Yeah that's the risk of anything processed - and what a good reagent test kit helps with.
|
YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,202
Loc: Castles made of sand
Last seen: 7 hours, 4 minutes
|
Re: Cultivation General Discussion [Re: wavyedge]
#28574452 - 12/08/23 01:56 PM (1 month, 19 days ago) |
|
|
I'll have to find it but one process to isolate psilocybin uses glacial acetic acid then I believe methylchloride?
|
stockw

Registered: 08/31/21
Posts: 450
|
Re: Cultivation General Discussion [Re: TheOffice]
#28574456 - 12/08/23 01:59 PM (1 month, 19 days ago) |
|
|
Yeah, they're incredible. It's my main motivation to understand the process more. Me & my housemates at the time would buy a bottle once a month or more depending on how much we were partying. But this guy sold a 50ml dropper bottle for 100 quid. 3 drops would get you nice, 6 would get you flying. Overall it was an amazing product and I regret not asking him more questions. I strive to recreate a product of similar quality.
(It was definitely psilocybin)
|
thirdeyewild



Registered: 11/01/13
Posts: 794
Loc:
|
Re: Cultivation General Discussion [Re: stockw]
#28574463 - 12/08/23 02:03 PM (1 month, 19 days ago) |
|
|
Never heard of this but I'm intrigued. The biggest barrier to tripping for me is the thought of chocking back the tea or fresh shrooms. I should probably try gummies one of these days.
|
YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,202
Loc: Castles made of sand
Last seen: 7 hours, 4 minutes
|
Re: Cultivation General Discussion [Re: stockw] 3
#28574467 - 12/08/23 02:06 PM (1 month, 19 days ago) |
|
|
Gummies are awesome 3rdEye!
Here is the extraction article.
From
https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/chem.201904363
"Natural product extraction. Initially, mycelia and carpophores were lyophilized, ground, and extracted with anhydrous MeOH, as described to extract 1 gently and to minimize its artificial dephosphorylation to 2. [4c] For improved carboline yields, the fungal biomasses (mycelia, carpophores, or sclerotia) were lyophilized, pulverized, and the powder solved in 0.1 M HCl and subsequently extracted with methylene chloride (1:1, v/v). The aqueous phases were collected, the pH value adjusted to 12 using NaOH, which was followed by extractions with methylene chloride. The organic phases were dried under reduced pressure in a rotary evaporator. The resulting crude extracts were dissolved in methanol, centrifuged and filtered, and subsequently used for chromatographic analysis or purification. To quantify 4 titers in fungal biomass, the areas under the curve (AUCs) in the extracted ion chromatograms were determined and referenced to a standard curve recorded with authentic 4. Chromatographic purification of 4 and 5. Preparative HPLC was performed using an Agilent 1260 instrument equipped with Phenomenex Luna C18 column (250 × 21.2 mm, 10 µm particle size), and run with 0.1% trifluoroacetic acid (TFA) in water (solvent A) and acetonitrile (ACN, solvent B). The flow was 20 mL min–1. A linear gradient was applied with an increase from 10 to 100% B within 20 min. β-carbolines were further purified by semipreparative HPLC using an Agilent 1200 instrument equipped with a Zorbax Eclipse XDB-C18 column (250 × 9.4 mm, 5 µm). and the same solvents, applying a flow of 2 mL min–1 and a linear gradient from 10 to 100% B within 10 min. The final purification was accomplished with the same solvents and instrument, but using a Phenomenex Synergi RP-80 column (250 × 10 mm, 4 µm) and a gradient that included an initial hold at 30% B for 1 min, an increase to 65% B within 10 min, and to 100% B within further 30 sec. This procedure yielded 3.5 mg of 4 and 14.4 mg of 5, which were dissolved in DMSO-d6 for subsequent NMR analysis (below). HPLC and mass spectrometry. HPLC and mass spectrometry were performed on a Thermo Accela liquid chromatograph equipped with a C18 column (Grom-Sil 100 ODS-0 AB, 250 × 4.6 mm, 3 µm) fitted to an Exactive Orbitrap spectrometer, using electrospray ionization. The respective diode array detectors covered the wavelength range of λ=200–400 nm. Initially, HPLC-UV chromatograms were extracted at λ=280 nm (to detect 1), later at λ=300 nm to detect β-carbolines. Conditions for HPLC included solvents 0.1% TFA in water (A) and 0.1% TFA in ACN (B) at a flow rate of 0.4 mL min–1. The gradient was: initial hold at 10% B for 1 min, and linear increase to 98% B within 4 min. Standard analytical runs were performed on a Thermo Vanquish Horizon UHPLC system equipped with a diode array and a fluorescence detector. This instrument was equipped with a Phenomenex Kinetex XB-C18 column (100 × 2.1 mm, 1.7 µm particle size). For fluorescence detection, excitation and emission were at λ=340 and 410 nm, respectively. Solvents were 0.1% formic acid (FA) in water (A) and ACN (B) at a flow rate of 1 mL min–1. The gradient was: initial hold at 5% B for 1 min, and linear increase to 100% B within 15 min. Chromatography and mass spectrometry to quantify the concentration of 4 was done on an Agilent 1290 Infinity II UHPLC instrument with a diode array detector (DAD) and interfaced to a 6130 quadrupole mass detector, run in ESI mode. The chromatograph was equipped with a Phenomenex Luna Omega Polar C18 50 × 2.1 mm (1.6 µm particle size) and a guard column. Separation was at 25°C and a flow of 0.5 mL min-1. Mobile phase A was 0.1% aqueous FA, phase B was ACN + 0.1% FA. A linear gradient was applied (% B): initially 1%, within 3 min to 10%, and within further 1 min to 100%. UV/Vis spectra were recorded with the diode-array detector during LCMS analyses. Samples were dissolved in MeOH. MALDI-MS imaging. P. cubensis mycelium was directly"
|
YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,202
Loc: Castles made of sand
Last seen: 7 hours, 4 minutes
|
Re: Cultivation General Discussion [Re: YoshiTrainer]
#28574471 - 12/08/23 02:10 PM (1 month, 19 days ago) |
|
|
StockW, you can make some pretty strong extracts, depending on starting amount of mush and how much you reduce the extraction later. Did the drops taste like anything? Heavily concentrated mushroom extract can taste nasty!
|
wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
|
Re: Cultivation General Discussion [Re: YoshiTrainer] 1
#28574479 - 12/08/23 02:21 PM (1 month, 19 days ago) |
|
|
DCM is nasty stuff, probably carcinogenic. For DMT and mescaline, extraction is really a necessity IMO, but from what procedures I've seen I'm not confident about extracting psilocybin, and the mushrooms are already so potent.
|
TheOffice
Stranger



Registered: 05/13/21
Posts: 83
Loc: The Netherlands
Last seen: 3 hours, 7 minutes
|
Re: Cultivation General Discussion [Re: Smellyhobbit]
#28574488 - 12/08/23 02:24 PM (1 month, 19 days ago) |
|
|
Quote:
Smellyhobbit said: I doubt that’s psilocybin in those drops
I also have my doubts,
But as Stockw said, there are several drops sold as psilocybin drops.
What would be your argument(s) for your doubt(s)?
-------------------- Cum grege non gradior
|
TheOffice
Stranger



Registered: 05/13/21
Posts: 83
Loc: The Netherlands
Last seen: 3 hours, 7 minutes
|
Re: Cultivation General Discussion [Re: YoshiTrainer]
#28574495 - 12/08/23 02:28 PM (1 month, 19 days ago) |
|
|
Quote:
YoshiTrainer said: Gummies are awesome 3rdEye!
Here is the extraction article.
From
https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/chem.201904363
"Natural product extraction. Initially, mycelia and carpophores were lyophilized, ground, and extracted with anhydrous MeOH, as described to extract 1 gently and to minimize its artificial dephosphorylation to 2. [4c] For improved carboline yields, the fungal biomasses (mycelia, carpophores, or sclerotia) were lyophilized, pulverized, and the powder solved in 0.1 M HCl and subsequently extracted with methylene chloride (1:1, v/v). The aqueous phases were collected, the pH value adjusted to 12 using NaOH, which was followed by extractions with methylene chloride. The organic phases were dried under reduced pressure in a rotary evaporator. The resulting crude extracts were dissolved in methanol, centrifuged and filtered, and subsequently used for chromatographic analysis or purification. To quantify 4 titers in fungal biomass, the areas under the curve (AUCs) in the extracted ion chromatograms were determined and referenced to a standard curve recorded with authentic 4. Chromatographic purification of 4 and 5. Preparative HPLC was performed using an Agilent 1260 instrument equipped with Phenomenex Luna C18 column (250 × 21.2 mm, 10 µm particle size), and run with 0.1% trifluoroacetic acid (TFA) in water (solvent A) and acetonitrile (ACN, solvent B). The flow was 20 mL min–1. A linear gradient was applied with an increase from 10 to 100% B within 20 min. β-carbolines were further purified by semipreparative HPLC using an Agilent 1200 instrument equipped with a Zorbax Eclipse XDB-C18 column (250 × 9.4 mm, 5 µm). and the same solvents, applying a flow of 2 mL min–1 and a linear gradient from 10 to 100% B within 10 min. The final purification was accomplished with the same solvents and instrument, but using a Phenomenex Synergi RP-80 column (250 × 10 mm, 4 µm) and a gradient that included an initial hold at 30% B for 1 min, an increase to 65% B within 10 min, and to 100% B within further 30 sec. This procedure yielded 3.5 mg of 4 and 14.4 mg of 5, which were dissolved in DMSO-d6 for subsequent NMR analysis (below). HPLC and mass spectrometry. HPLC and mass spectrometry were performed on a Thermo Accela liquid chromatograph equipped with a C18 column (Grom-Sil 100 ODS-0 AB, 250 × 4.6 mm, 3 µm) fitted to an Exactive Orbitrap spectrometer, using electrospray ionization. The respective diode array detectors covered the wavelength range of λ=200–400 nm. Initially, HPLC-UV chromatograms were extracted at λ=280 nm (to detect 1), later at λ=300 nm to detect β-carbolines. Conditions for HPLC included solvents 0.1% TFA in water (A) and 0.1% TFA in ACN (B) at a flow rate of 0.4 mL min–1. The gradient was: initial hold at 10% B for 1 min, and linear increase to 98% B within 4 min. Standard analytical runs were performed on a Thermo Vanquish Horizon UHPLC system equipped with a diode array and a fluorescence detector. This instrument was equipped with a Phenomenex Kinetex XB-C18 column (100 × 2.1 mm, 1.7 µm particle size). For fluorescence detection, excitation and emission were at λ=340 and 410 nm, respectively. Solvents were 0.1% formic acid (FA) in water (A) and ACN (B) at a flow rate of 1 mL min–1. The gradient was: initial hold at 5% B for 1 min, and linear increase to 100% B within 15 min. Chromatography and mass spectrometry to quantify the concentration of 4 was done on an Agilent 1290 Infinity II UHPLC instrument with a diode array detector (DAD) and interfaced to a 6130 quadrupole mass detector, run in ESI mode. The chromatograph was equipped with a Phenomenex Luna Omega Polar C18 50 × 2.1 mm (1.6 µm particle size) and a guard column. Separation was at 25°C and a flow of 0.5 mL min-1. Mobile phase A was 0.1% aqueous FA, phase B was ACN + 0.1% FA. A linear gradient was applied (% B): initially 1%, within 3 min to 10%, and within further 1 min to 100%. UV/Vis spectra were recorded with the diode-array detector during LCMS analyses. Samples were dissolved in MeOH. MALDI-MS imaging. P. cubensis mycelium was directly"
This is all about extraction and identification right?
Im not sure extraction is the problem but storing it and keeping it shelf stable. And I mean in drop bottles you can bring to a party and staying potent for some weeks/months. (Not like in a freezer at -80 °C or something)
P.s. I know for a fact some vendors also put 4-aco-dmt in the bottles.. to "supplement the psilocybin" could this be a stabilizing factor or is it just to make up for the degradation of psilocybin/psilocin?
-------------------- Cum grege non gradior
Edited by TheOffice (12/08/23 02:31 PM)
|
YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,202
Loc: Castles made of sand
Last seen: 7 hours, 4 minutes
|
Re: Cultivation General Discussion [Re: wavyedge] 1
#28574502 - 12/08/23 02:30 PM (1 month, 19 days ago) |
|
|
I agree Wavy, DCM is nasty! For my purposes, citric acid + water works very well! If you want portability, make gummies or even chocolates, nice and easy!
|
San Pedro Girl
Shoebox Ninja🥷




Registered: 07/17/12
Posts: 2,385
Loc: Fuck off pig!🐷
Last seen: 12 hours, 54 minutes
|
Re: Cultivation General Discussion [Re: YoshiTrainer] 3
#28574510 - 12/08/23 02:33 PM (1 month, 19 days ago) |
|
|
Quote:
YoshiTrainer said: I agree Wavy, DCM is nasty! For my purposes, citric acid + water works very well! If you want portability, make gummies or even chocolates, nice and easy!
I make spearmint tea with lemon and honey. It completely masks the taste.
|
stockw

Registered: 08/31/21
Posts: 450
|
Re: Cultivation General Discussion [Re: YoshiTrainer] 1
#28574511 - 12/08/23 02:33 PM (1 month, 19 days ago) |
|
|
Quote:
YoshiTrainer said: StockW, you can make some pretty strong extracts, depending on starting amount of mush and how much you reduce the extraction later. Did the drops taste like anything? Heavily concentrated mushroom extract can taste nasty!
Yes Yoshi! Good to see ya. They tasted bitter, really bitter. Colour was dark & rich, slightly viscous.
|
YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,202
Loc: Castles made of sand
Last seen: 7 hours, 4 minutes
|
Re: Cultivation General Discussion [Re: stockw]
#28574552 - 12/08/23 03:04 PM (1 month, 19 days ago) |
|
|
Might have been the real deal!?
|
Nichrome
I'm a torso!


Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 7 hours, 10 minutes
|
Re: Cultivation General Discussion [Re: mrmazdarx9] 4
#28574635 - 12/08/23 04:21 PM (1 month, 19 days ago) |
|
|
Quote:
mrmazdarx9 said: No tens damn sexy monos worthy of a 10 make you poke the Poly out 
Trying to use a translator to ask questions in an English speaking forum.
I think what you are asking for is advice in going straight to fruiting conditions with a monotub. In that case, just do it. That's what everybody does.
Once you spawn (dump mycelium into a tub) it's proper practice to expose the tub to fresh air exchange and light cycles without delay.
Good luck with the language berrier and welcome to the Shroomery.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
|
Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,379
|
Re: Cultivation General Discussion [Re: Nichrome] 1
#28574659 - 12/08/23 04:39 PM (1 month, 19 days ago) |
|
|
Quote:
Nichrome said:
Quote:
mrmazdarx9 said: No tens damn sexy monos worthy of a 10 make you poke the Poly out 
Trying to use a translator to ask questions in an English speaking forum.
I think what you are asking for is advice in going straight to fruiting conditions with a monotub. In that case, just do it. That's what everybody does.
Once you spawn (dump mycelium into a tub) it's proper practice to expose the tub to fresh air exchange and light cycles without delay.
Good luck with the language berrier and welcome to the Shroomery.
This is from page one homie, seven years ago
--------------------
🅃🄴🄰🄼 🄵🄾🄸🄻
|
HILLBILLY OUTLAW
Above And Beyond!



Registered: 04/21/22
Posts: 2,852
Loc: Luckenbach Texas
|
Re: Cultivation General Discussion [Re: Inocuole] 1
#28574794 - 12/08/23 06:41 PM (1 month, 19 days ago) |
|
|
He’s testing you and you passed.
--------------------
 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 TEAM SPREAD THE LOVE! Smellyhobbit said: Embarrassment and bashfulness are leeches on your ability to learn.
|
Nichrome
I'm a torso!


Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 7 hours, 10 minutes
|
|
Left the dude hangin' for 7 years.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
|
tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 1 day, 20 hours
|
Re: Cultivation General Discussion [Re: Nichrome] 5
#28575263 - 12/09/23 03:51 AM (1 month, 19 days ago) |
|
|
Smokes, let's go

-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
|
Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 20 hours, 55 minutes
|
|
Quote:
San Pedro Girl said: People have been isolating psilocybin from shrooms since the 1950s, but you think it can’t be done now? Hot water extraction is a fine way to make a tea, but it’s nowhere near pure. Reduce and try to crystallize and see what kind of trash you get.
I haven't made myself clear, I do think psilocybin can be extracted from mushrooms. I have no doubt, I have seen it described. What I do doubt is:
(1) If we could isolate other alkaloids such as baeocystin or aeruginascin and if they are even active and/or can be differentiated (subjectively) from psilocybin? I have not seen any techniques to extract these or experiences with them.
(2) If a psilocybin extraction can be differentiated from a mushroom experience including other alkaloids
And then another point that I have is why would you bother with a full spectrum (so, with all alkaloids present) mushroom extraction vs a hot water extraction? Isn't a hot water extraction basically a full spectrum extraction? For where I am standing I have reduced hot water extractions down to 1g/mL or more, which is a crazy potent liquid. I do not see any point for stronger extractions for the vast majority (99.9%+) of users.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
|
|