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JW123
Nooby Nooby Noob


Registered: 01/17/22
Posts: 1,303
Loc: Tn
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Re: Cultivation General Discussion [Re: MLPismyOPSEC] 1
#28541354 - 11/13/23 10:58 AM (2 months, 13 days ago) |
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Happy shroomery birthday.
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TheAtlantean
Forever Grateful


Registered: 06/25/18
Posts: 1,000
Loc: Milkyway Galaxy, Laniakea Supe...
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#28541406 - 11/13/23 11:54 AM (2 months, 13 days ago) |
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Quote:
MLPismyOPSEC said: Do we have any really good resources for brushing up on our transferring tek? Been fighting contam in my cultures for two years now and i'm slowly winning the battle but it's very frustrating. I've used water agar to try to clean them up to no avail. Makes me wonder if it's time to nut up and get a microscope but i'm assuming i just need to git gud. People shooting MSS into grain jars are probably having better success than i am at the moment 
Edit: holy shit i've been around for 5 years now 
TheAtlantean’s Cloning/Agar Tek
-------------------- Meditation is passive learning. Tabernacling with the one infinite Creator is passive learning. It is the learning from listening to the voice one cannot hear which answers questions which one cannot ask. The process cannot be known and cannot come into consciousness in local sense, yet the learning is there. And the strength of spirit and will which comes from this learning is helpful. . www.templeofhermes.org
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San Pedro Girl
Shoebox Ninja🥷




Registered: 07/17/12
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Re: Cultivation General Discussion [Re: TheAtlantean] 2
#28546422 - 11/17/23 11:48 AM (2 months, 9 days ago) |
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jmycology
johnnymyco
Registered: 11/05/23
Posts: 96
Last seen: 1 hour, 21 minutes
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hey im looking for a site to buy the agar cups not the dishes anyone have any recommendations
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jmycology
johnnymyco
Registered: 11/05/23
Posts: 96
Last seen: 1 hour, 21 minutes
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Re: Cultivation General Discussion [Re: jmycology]
#28546466 - 11/17/23 12:27 PM (2 months, 9 days ago) |
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what do i get as far as agar with or without antibiotics?? im new to agar
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jungatheart
Stranger

Registered: 06/11/22
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Re: Cultivation General Discussion [Re: jmycology]
#28546472 - 11/17/23 12:46 PM (2 months, 9 days ago) |
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Quote:
jmycology said: hey im looking for a site to buy the agar cups not the dishes anyone have any recommendations
pp5 cups and lids here
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Smellyhobbit
Actual Retard



Registered: 04/01/22
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Re: Cultivation General Discussion [Re: jmycology] 2
#28546473 - 11/17/23 12:48 PM (2 months, 9 days ago) |
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Quote:
jmycology said: what do i get as far as agar with or without antibiotics?? im new to agar
Without
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Alifebuggin
Stranger

Registered: 02/27/21
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Re: Cultivation General Discussion [Re: Smellyhobbit] 2
#28549943 - 11/20/23 08:24 AM (2 months, 6 days ago) |
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Shake day it looks like on a round of qt jars!  Pretty happy with speed this is 4-5days.
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,488
Loc: USA
Last seen: 11 seconds
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Re: Cultivation General Discussion [Re: jungatheart] 1
#28549952 - 11/20/23 08:37 AM (2 months, 6 days ago) |
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Quote:
jungatheart said:
Quote:
jmycology said: hey im looking for a site to buy the agar cups not the dishes anyone have any recommendations
pp5 cups and lids here
You can also use these
Comfy Package [200 Sets - 2 oz.] Plastic Disposable Portion Cups with Lids, Souffle Cups, Jello Cups https://a.co/d/g82Memy
I love them. I don’t pre pour either. Pour them after PCing the agar. I don’t sterilize the cups and do a shitty, half ass job pouring them in an Sab. I lose like 3 cups out of like 80 poured lol. Brushing up my technique could probably help that too. But just pointing out you don’t need to do them pre pour style
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,204
Last seen: 18 hours, 58 minutes
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Quote:
SupaThaRipper said:
Quote:
jungatheart said:
Quote:
jmycology said: hey im looking for a site to buy the agar cups not the dishes anyone have any recommendations
pp5 cups and lids here
You can also use these
Comfy Package [200 Sets - 2 oz.] Plastic Disposable Portion Cups with Lids, Souffle Cups, Jello Cups https://a.co/d/g82Memy
I love them. I don’t pre pour either. Pour them after PCing the agar. I don’t sterilize the cups and do a shitty, half ass job pouring them in an Sab. I lose like 3 cups out of like 80 poured lol. Brushing up my technique could probably help that too. But just pointing out you don’t need to do them pre pour style
do you cling wrap the lid? how easy does the agar puck fall out of the container?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,488
Loc: USA
Last seen: 11 seconds
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Re: Cultivation General Discussion [Re: tedoro]
#28549984 - 11/20/23 09:11 AM (2 months, 6 days ago) |
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Nah man, no cling wrap, no parafilm, no nothing. You just flip it upside down. And give it a light squeeze on the sides of the cup and the agar just pops right out, easy as hell. I love using them
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Alifebuggin
Stranger

Registered: 02/27/21
Posts: 69
Last seen: 1 month, 7 days
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Can I ask why you dont wrap them at all?
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,488
Loc: USA
Last seen: 11 seconds
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Re: Cultivation General Discussion [Re: Alifebuggin]
#28550003 - 11/20/23 09:26 AM (2 months, 6 days ago) |
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Quote:
Alifebuggin said: Can I ask why you dont wrap them at all?
I first got the idea from this guy
https://instagram.com/monokaryonmywaywardson?igshid=YTQwZjQ0NmI0OA==
He does some absolutely beautiful work. And if he doesn’t wrap them I’m not gonna 🤷♂️ haha. It also has proven to be just unnecessary. Haven’t had any issues at all
And just to clarify, I still wrap my regular petris. The lids on the condiment cups do snap on
Edited by SupaThaRipper (11/20/23 09:27 AM)
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Alifebuggin
Stranger

Registered: 02/27/21
Posts: 69
Last seen: 1 month, 7 days
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Quote:
SupaThaRipper said:
Quote:
Alifebuggin said: Can I ask why you dont wrap them at all?
I first got the idea from this guy
https://instagram.com/monokaryonmywaywardson?igshid=YTQwZjQ0NmI0OA==
He does some absolutely beautiful work. And if he doesn’t wrap them I’m not gonna 🤷♂️ haha. It also has proven to be just unnecessary. Haven’t had any issues at all
And just to clarify, I still wrap my regular petris. The lids on the condiment cups do snap on
Ohhhh okay I thought you were saying you dont even use the lids and I was like "hmmm, not sure how great that would be". But yeah the lids snap on and are tight.
I ordered a bucnh of these just froma different seller and the lids are not pp5 so I had to send them back as I couldnt throw them in the PC. The one you listed you persoally PC the lids and cups?
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,488
Loc: USA
Last seen: 11 seconds
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Re: Cultivation General Discussion [Re: Alifebuggin] 1
#28550032 - 11/20/23 09:47 AM (2 months, 6 days ago) |
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No I don’t sterilize the cups or lids. I pour them as them come. I open the packaging inside my SAB and pour them all in there. And then just tie the bag shut inside the SAB if there’s any left. Haven’t had anymore issues doing it this way than when I do them with petris
I also don’t even use arm holes on my SAB. I just flip a tote on its side and leave the whole front open lol. So the success is pretty damn high considering my lack of care
Edited by SupaThaRipper (11/20/23 09:48 AM)
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Alifebuggin
Stranger

Registered: 02/27/21
Posts: 69
Last seen: 1 month, 7 days
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Quote:
SupaThaRipper said: No I don’t sterilize the cups or lids. I pour them as them come. I open the packaging inside my SAB and pour them all in there. And then just tie the bag shut inside the SAB if there’s any left. Haven’t had anymore issues doing it this way than when I do them with petris
I also don’t even use arm holes on my SAB. I just flip a tote on its side and leave the whole front open lol. So the success is pretty damn high considering my lack of care
I mean if it works then it works I suppose. My enviormemt would never be conducive to that but if it’s working for you then that’s works!
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
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Re: Cultivation General Discussion [Re: Alifebuggin] 4
#28550043 - 11/20/23 10:01 AM (2 months, 6 days ago) |
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Dude I do all my work in an unfinished basement, 20’ from 3 cat litter boxes. I have 3 cats, and 3 dogs, and now 5 more puppies. I have 2 sloppy kids as well. My environment is as far from sanitary as possible
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 15 seconds
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Yeah the table I do my SAB sessions on, besides the immediate area, is not in any way clean.
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Alifebuggin
Stranger

Registered: 02/27/21
Posts: 69
Last seen: 1 month, 7 days
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Quote:
SupaThaRipper said: Dude I do all my work in an unfinished basement, 20’ from 3 cat litter boxes. I have 3 cats, and 3 dogs, and now 5 more puppies. I have 2 sloppy kids as well. My environment is as far from sanitary as possible
Sounds like my home enviornment too...! I mean I generally havent ever had any contamination issue with how I work except for lets say contaminated stock (print,LC, etc, bad grain PC, etc) material, but I never seen how far I could push my home set up like you described. I built a flow hood I used to use for work and use that with no extra precuations I suppose.
But your success in just your method I enjoy hearing. I'll have to reach out to you sometime just to chat about this more.
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,488
Loc: USA
Last seen: 11 seconds
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Re: Cultivation General Discussion [Re: Alifebuggin]
#28550090 - 11/20/23 10:33 AM (2 months, 6 days ago) |
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Anytime man
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