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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: nobody83]
    #23653016 - 09/17/16 12:27 PM (7 years, 4 months ago)

I never read its good to switch up agar till recently i i was running pda for about a month then mea for two now i have both in quart jars so i can switch my clones back and fourth its cool how that works ... and i usually run my hood for atleast thirty minites before i do anything but have been in a rush a few times and just turned it on and got to work with no issues i just started making prints so its new to me i mean i still have 10 years worth of syringe solution haha but figured id make prints in case something happened... supa ive got foil trays with lids and 6 qt tubs i should use those then right?? Thanks for the help


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InvisibleSupalemonhaze
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23653165 - 09/17/16 01:38 PM (7 years, 4 months ago)

If they are big enough, sure. I prefer stainless steel trays that are about as wide as my filter but you can use anything you like. The point is to leave none of the open spaces between the prints exposed to contams. If you were printing in a SAB, a jar over the cap works great since you are dealing with still air but a hood will be blowing across your prints so anything laden with mold spores between your filter and your print should be avoided. Covering your tray just before you switch of your hood will keep the inside of your tray/the rest of the foil as clean as possible.


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Supalemonhaze]
    #23653176 - 09/17/16 01:41 PM (7 years, 4 months ago)

Right on man thanks hows things with you dude ??


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InvisibleSupalemonhaze
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23653248 - 09/17/16 02:16 PM (7 years, 4 months ago)

I'm alright, just working my ass off. Been reading TMC and GGMM in the little free time I have so I haven't been around here much. Good to see your grows going strong on POTD btw. Grats.


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Supalemonhaze]
    #23653355 - 09/17/16 03:02 PM (7 years, 4 months ago)

Thanks man you helped alot bro


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OfflineRooster Cogburn
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Re: Cultivation General Discussion [Re: Supalemonhaze]
    #23653366 - 09/17/16 03:05 PM (7 years, 4 months ago)

Quote:

Supalemonhaze said:
I'm alright, just working my ass off. Been reading TMC and GGMM in the little free time I have so I haven't been around here much. Good to see your grows going strong on POTD btw. Grats.




Shroomery blasphemy Haze. JK, I keep GGMM in my bathroom, I swear to god every time I take a shit I learn something new.
In all seriousness, what are you reading up on right now? I was just reading the section on Agiricus Blazei and he very briefly mentions Japanese cultivators having success with them on 20% supplemented sawdust bags, but absolutely no other further info on that. Interesting though is he did mention the pigment and colorization of the fruit bodies being completely different on sawdust than compost and said he felt substrate could play a huge role in pigment and possibly taxonomy of species has been skewed by this. Anyways, that was my morning GGMM reading, I was thinking about trying blazei on coir so I was reading up on it, apparently it wont fruit though unless the casing is untreated and has bacteria present, I'm wondering if coir casing untreated would do the trick?


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23653370 - 09/17/16 03:07 PM (7 years, 4 months ago)

are these pre sterilized? I bought it from my feed shop with a 18 gauge needle for my li


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OfflineSloppyJoseph
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23653384 - 09/17/16 03:14 PM (7 years, 4 months ago)

I mean I wouldn't risk it. If it's reusable you are going to need to re sterilize it anyway so you might as well do it the first time. Suck up and squirt out boiling hot water 5 or 6 times. Sometimes people PC their syringes but I've never so I can't say how you would go about doing it


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: SloppyJoseph]
    #23653406 - 09/17/16 03:19 PM (7 years, 4 months ago)

I just wrap in foil but it wpuld be safe before losing 40 jars haha i just was checkin if they were good on the firsr go thanks


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Offlinetombosley8
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Re: Cultivation General Discussion [Re: SloppyJoseph]
    #23653420 - 09/17/16 03:23 PM (7 years, 4 months ago)

I just wrap mine in foil and pc.


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OfflineDactylium
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Re: Cultivation General Discussion [Re: tombosley8]
    #23653526 - 09/17/16 04:02 PM (7 years, 4 months ago)

I've been having a recurring nightmare where I open my petris outside of my still air box and fuck all my cultures up before I realize what I'm doing. Does this happen to anyone else?


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InvisibleInocuole
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Re: Cultivation General Discussion [Re: Dactylium]
    #23653533 - 09/17/16 04:04 PM (7 years, 4 months ago)

:lol:  No.  But that's pretty funny.  Just don't do agar work while you're sleepy or in any kinda daze.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleliloldmeFacebook
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Re: Cultivation General Discussion [Re: Inocuole]
    #23653602 - 09/17/16 04:31 PM (7 years, 4 months ago)

That sounds like a pretty tame nightmare.


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OfflineKenetic
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Re: Cultivation General Discussion [Re: liloldme]
    #23653769 - 09/17/16 05:32 PM (7 years, 4 months ago)

I've had worse


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Kenetic]
    #23654179 - 09/17/16 09:33 PM (7 years, 4 months ago)

i had another thread going but i open aired this jar

and within 10min it went to this.

Ive never had trich before but this looks just like bruising.
or do i need to toss this asap?


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: mushboy]
    #23654186 - 09/17/16 09:38 PM (7 years, 4 months ago)

Looks like bruising to me man


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OfflineKenetic
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23654247 - 09/17/16 10:02 PM (7 years, 4 months ago)

trich isn't gonna turn green in ten minutes.  It may turn green overnight, but not in ten minutes.  sounds and looks like bruising.  The surface looks too shiny also.  could be dry

I wonder why it bruised though, interesting.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Kenetic]
    #23654254 - 09/17/16 10:05 PM (7 years, 4 months ago)

Maybe it was too dry and the air hitting it bruised it plus misting ??


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InvisibleSupalemonhaze
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23654734 - 09/18/16 02:45 AM (7 years, 4 months ago)

Quote:

Rooster Cogburn said:
Quote:

Supalemonhaze said:
I'm alright, just working my ass off. Been reading TMC and GGMM in the little free time I have so I haven't been around here much. Good to see your grows going strong on POTD btw. Grats.




Shroomery blasphemy Haze. JK, I keep GGMM in my bathroom, I swear to god every time I take a shit I learn something new.
In all seriousness, what are you reading up on right now? I was just reading the section on Agiricus Blazei and he very briefly mentions Japanese cultivators having success with them on 20% supplemented sawdust bags, but absolutely no other further info on that. Interesting though is he did mention the pigment and colorization of the fruit bodies being completely different on sawdust than compost and said he felt substrate could play a huge role in pigment and possibly taxonomy of species has been skewed by this. Anyways, that was my morning GGMM reading, I was thinking about trying blazei on coir so I was reading up on it, apparently it wont fruit though unless the casing is untreated and has bacteria present, I'm wondering if coir casing untreated would do the trick?




I'm actually on the grain spawn part of both books. I bought GGMM first as it's easier to source so I started reading that. Then I recieved TMC and decided to start that one since GGMM is meant to be a continuation.

As for your blazei project, I think that a specific type(s) of bacteria is needed if he mentions that in his book. Not any random bacteria is gonna work probably. Also, I wouldn't at all be surprised if it doesn't work with coir, I think the best chance you would have is with a peat based casing. But definitely, for learning's sake, do them both. Or do some research on the net and try to find out what kind of bacteria it needs and what it is naturally found in.

Quote:

noob47 said:
are these pre sterilized? I bought it from my feed shop with a 18 gauge needle for my li




If it's sterile, it should have a box with "sterile EO" written on it. If it doesn't, I would sterilize it how sloppy mentioned. Whenever I tried to PC my syringes, they always ended up leaking. Sometimes they would leak just ever so slightly, not enough to notice but more than enough for contams to pass through the gap. Took me a while to figure out why all my jars were going green. That's when I decided to boycott syringes and ports. A2G FTFW!


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Supalemonhaze]
    #23655202 - 09/18/16 10:13 AM (7 years, 4 months ago)

Damn thats shitty as fuck dude i hate working with syringes also i always am worried ill waste grain ..  but pouring the li out of the jar i feel i may pour too much i know in the tek it says 25-50 ml and i dont think ive gone over that amount at all but still


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