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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Re: Cultivation General Discussion [Re: Paperbagboy]
#24216550 - 04/03/17 09:51 PM (6 years, 9 months ago) |
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So here's a little experiment I just tried the other day.
Spawned a big monotub with clean spawn following regular procedures
Then I spawned a big monotub but I decided to do it a bit differe: -I poured all the blended spawn/sub into a clear trash bag and then a knot in the bag closing it off completely and tossed it into a monotub. -The plan is to let it colonize fully and then cut the bag open and fruit it -I was hoping that by bagging the substrate it would be like the opposite of fruiting at spawn, and would promote strong mycelial growth and allow me to decide how long I wanted to wait before triggering pinning.
And here's what happened: It's day 3 or 4 and it doesn't look good at all. The regular tub is colonizing really well and this bagged up one that literally has zero FAE, has no visible myclelium growth. My bet is it's all going anaerobic and I'm going to have a fat sack of smelly bacteria. It's also bloated up and looks like the bag is starting to build pressure.
Just thought I would share my results
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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The lack of GE probably killed it off good thoughts though.
I'm going to do one myself soon since I'm struggling to recognize bacterial spawn.
I'm going to take a plate with different amounts of bacteria on them and throw them on grain to see how it looks. Since I know 100℅ it's contaminated with bacteria I could recognize the signs easier.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Cultivation General Discussion [Re: van hatton]
#24216910 - 04/04/17 01:33 AM (6 years, 9 months ago) |
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That's clever as fuck. All I know to do is shake the bastards a couple times and if they don't recover, they bad.
-------------------- JOIN THE POW WOW
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 6 hours
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Got somethin I've probably been over thinking and would love some advice
After the sterilization cycle and after cooling I am always worried about moving the pc or sterilizer while the pp5 lids are not tightly screwed on. thus I have been opening them in the kitchen before moving them to tighten the lids and then moving the pc to my sab/flowhood.
Will moving the pc's while the pp5 lids are loose cause enough air current to push any contams sucked in while cooling through the loose lids ?
or is the inside of the pc/sterilizer pretty current free while moving so I wouldn't have to tighten the lids first?
I just hate having to open the pc twice and expose them to any extra contams. It's best going from an unopened pc/sterilizer directly into the sab/flow imo.
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Timmy Meow



Registered: 04/05/15
Posts: 546
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Quote:
Thedillestpickle said: So here's a little experiment I just tried the other day.
Spawned a big monotub with clean spawn following regular procedures
Then I spawned a big monotub but I decided to do it a bit differe: -I poured all the blended spawn/sub into a clear trash bag and then a knot in the bag closing it off completely and tossed it into a monotub. -The plan is to let it colonize fully and then cut the bag open and fruit it -I was hoping that by bagging the substrate it would be like the opposite of fruiting at spawn, and would promote strong mycelial growth and allow me to decide how long I wanted to wait before triggering pinning.
And here's what happened: It's day 3 or 4 and it doesn't look good at all. The regular tub is colonizing really well and this bagged up one that literally has zero FAE, has no visible myclelium growth. My bet is it's all going anaerobic and I'm going to have a fat sack of smelly bacteria. It's also bloated up and looks like the bag is starting to build pressure.
Just thought I would share my results
Thanks for sharing.
I've made some tubs airtight and haven't had a problem with it colonizing like that, but upon opening the container I got this really strong fumes that I'm guessing is the carbon dioxide and what ever else it produces. Those fumes were potent AF
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Josh.0
ConnoissurOfSorts


Registered: 11/25/13
Posts: 553
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Re: Cultivation General Discussion [Re: tombosley8]
#24218975 - 04/04/17 08:39 PM (6 years, 9 months ago) |
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Quote:
tombosley8 said: Got somethin I've probably been over thinking and would love some advice
After the sterilization cycle and after cooling I am always worried about moving the pc or sterilizer while the pp5 lids are not tightly screwed on. thus I have been opening them in the kitchen before moving them to tighten the lids and then moving the pc to my sab/flowhood.
Will moving the pc's while the pp5 lids are loose cause enough air current to push any contams sucked in while cooling through the loose lids ?
or is the inside of the pc/sterilizer pretty current free while moving so I wouldn't have to tighten the lids first?
I just hate having to open the pc twice and expose them to any extra contams. It's best going from an unopened pc/sterilizer directly into the sab/flow imo.
Think of your PC as a sprayed sab..
Did that halp ease ya mind?
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Re: Cultivation General Discussion [Re: Timmy Meow]
#24219332 - 04/04/17 11:59 PM (6 years, 9 months ago) |
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Quote:
Timmy Meow said:
Quote:
Thedillestpickle said: So here's a little experiment I just tried the other day.
Spawned a big monotub with clean spawn following regular procedures
Then I spawned a big monotub but I decided to do it a bit differe: -I poured all the blended spawn/sub into a clear trash bag and then a knot in the bag closing it off completely and tossed it into a monotub. -The plan is to let it colonize fully and then cut the bag open and fruit it -I was hoping that by bagging the substrate it would be like the opposite of fruiting at spawn, and would promote strong mycelial growth and allow me to decide how long I wanted to wait before triggering pinning.
And here's what happened: It's day 3 or 4 and it doesn't look good at all. The regular tub is colonizing really well and this bagged up one that literally has zero FAE, has no visible myclelium growth. My bet is it's all going anaerobic and I'm going to have a fat sack of smelly bacteria. It's also bloated up and looks like the bag is starting to build pressure.
Just thought I would share my results
Thanks for sharing.
I've made some tubs airtight and haven't had a problem with it colonizing like that, but upon opening the container I got this really strong fumes that I'm guessing is the carbon dioxide and what ever else it produces. Those fumes were potent AF
how air-tight was it? I had a hole free bag with a strong knot tied in it so 100% airtight. I also didn't leave much airspace within the bag, so it probably ran out of air to breath pretty fast especially since it had 10 qt of spawn inside.... 10 qt wasted I guess.
I might try a similar experiment but not tie the bag and see how that goes... just trying to play around with variables a bit
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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It would have worked just fine if you had poked holes in the bag, basic stuff ma dude
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Timmy Meow] 1
#24219346 - 04/05/17 12:08 AM (6 years, 9 months ago) |
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Quote:
Timmy Meow said:
Thanks for sharing.
I've made some tubs airtight and haven't had a problem with it colonizing like that, but upon opening the container I got this really strong fumes that I'm guessing is the carbon dioxide and what ever else it produces. Those fumes were potent AF
How the fuck are you making tubs air tight? And carbon dioxide is odorless.
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Re: Cultivation General Discussion [Re: Josex]
#24219353 - 04/05/17 12:12 AM (6 years, 9 months ago) |
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Quote:
Josex said: It would have worked just fine if you had poked holes in the bag, basic stuff ma dude 
I know, I just wanted to see what would happen you know. Plastic does let some small amount of gas to pass through it, so it might have been enough. It was not. actually I might give that a try next time, tie a knot, poke some holes. I went with completely tied off because it's a very repeatable procedure so I know whatever results I obtain will likely be repeatable.
Main thing is i just want to let a tub consolidate for a long time, like 4 weeks, then fruit. I want to test my hypothesis that if you let the sub "consolidate" it will break down the starch in the grain further and be ready to explode when you fruit it.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: zajcob]
#24219359 - 04/05/17 12:14 AM (6 years, 9 months ago) |
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Quote:
zajcob said: I still don't see the relevance of a filter here when you don't need gas exchange since it's just a container with water that is to be drawn into the syringe, unless it is for preventing a vacuum when opening in the SAB.
Also the implication that the boiled water in the pot would be such a great vector for a syringe in the scenario I described is lost on me the way Josex puts it. The pot's interior will have been steamed and the water beneath the surface would be virtually sterile prior to opening the lid inasmuch as boiling water sterilizes it, which seems contrary to what I read in the PC manual regarding bacteria. Anyway, sure, when you open the lid of the pot, contaminants will land on the surface of the water, but beneath the surface I don't see that contaminants would have infiltrated the water in the small time frame that you remove the lid, although withdrawing water beneath the surface could suck water from the surface into the syringe. So I see the possibility of a contamination via that route, but not one as extraordinary as described. Note that this is just speculation about a last minute method that could have been done to hasten a nearly finished SAB session. Of course in general for salvaging a syringe for future use or creating new ones I would be inclined to use an SAB and other complimentary steps.
Anyway, regarding agar wedges, didn't you say that you would noc up several jars with one dish? I have five decent looking clone transfers that I want to use to inoculate 10 rye jars from, but maybe save one for further transferring/storage, giving me maybe 4 dishes to work with. I want to get some monos rolling from the clone.
And while I'm at it, regarding CVG bulk, is it common practice to just squeeze excess water with one's hand to get it to field capacity if it is too saturated?
That's some long-winded piece of The Bull's Shit...
       
Just vibin' along...
Edited by Josex (04/05/17 12:41 AM)
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Josex]
#24219384 - 04/05/17 12:45 AM (6 years, 9 months ago) |
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How wet is too wet with rye?
Just seems like I have a high moisture content which makes a good environment for bacteria.
No outside moisture boiled 30~ mins, steam dryed, loaded, and pced.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: van hatton]
#24219387 - 04/05/17 12:51 AM (6 years, 9 months ago) |
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Van, you really need to give this a try, you won't look back, trust me.
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Cultivation General Discussion [Re: Josex]
#24219617 - 04/05/17 06:12 AM (6 years, 9 months ago) |
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I was going to toss these dub tubs cause it looks like shit, but someone recommended flipping the sub. The tub pretty much look d like this

Now it's looking like this
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Josex]
#24219638 - 04/05/17 06:31 AM (6 years, 9 months ago) |
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I used to use that Tek actually I always felt like it was too dry after the PC
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: van hatton]
#24219707 - 04/05/17 07:19 AM (6 years, 9 months ago) |
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Sorry for double post but I can tell you guys all day.
But this is easier.

That's about a week old. Thoughts?
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Cultivation General Discussion [Re: van hatton]
#24219731 - 04/05/17 07:32 AM (6 years, 9 months ago) |
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How did you inoculate and what you growing? I believe some specifies are slower than others, usually within a few days I see some growth.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: abductee]
#24219784 - 04/05/17 07:58 AM (6 years, 9 months ago) |
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There's nothing in that jar 
Trying to find out if my grains moisture content is too high
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 8 hours, 59 minutes
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Re: Cultivation General Discussion [Re: van hatton]
#24219844 - 04/05/17 08:18 AM (6 years, 9 months ago) |
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Looks good to me.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: abductee]
#24219847 - 04/05/17 08:19 AM (6 years, 9 months ago) |
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They look good to me. I'd noc it up.
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