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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Even when I tried to loosely put them on I get a massive vacuum effect.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Cultivation General Discussion [Re: van hatton]
#24213060 - 04/02/17 11:55 AM (6 years, 9 months ago) |
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Haha for sure! I've preped grains and plates so I'm comfortable with the pc, but defiantly a good point. Nothing like fighting with a jar in a SAB, while your mother fucking the world the whole time. I can't wait to give it a shot. I've read and seen a lot of good things about LI, so when i get these plates grown and transferred so i can give it a go.
-------------------- Keep your feet on the ground and your head in the clouds
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ChadleyD
Spiralling out



Registered: 11/03/16
Posts: 19
Last seen: 5 years, 11 months
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Re: Cultivation General Discussion [Re: mushboy]
#24213393 - 04/02/17 02:28 PM (6 years, 9 months ago) |
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-------------------- Is there anybody out there?
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Cultivation General Discussion [Re: ChadleyD]
#24213470 - 04/02/17 03:04 PM (6 years, 9 months ago) |
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I have a very simple rule for my LI plates. if its anything besides a perfect circle, I don't use it. its a bit overkill, as sometimes pieces dont come off the blades just right, but i just reserve those plates for transfers. ideally, i have 2 plates for each culture. one plate for transferring, one plate for dropping into LI.
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,921
Loc:
Last seen: 21 hours, 26 minutes
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Re: Cultivation General Discussion [Re: ChadleyD]
#24213812 - 04/02/17 06:21 PM (6 years, 9 months ago) |
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Quote:
ChadleyD said:

Nice clusters man!
--------------------

Shadowboxing the apocalypse and wandering the land
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Crispykoot]
#24213916 - 04/02/17 07:08 PM (6 years, 9 months ago) |
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anyone mess around with surface dryness? as keep the sub wet with bottom watering but leave the surface slightly drier than normal?
well... the surface bruised and i cant notice much knotting.

the sides and bottom(nice and moist) have knots coming in nicely.
 (AA+)
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: zajcob]
#24213972 - 04/02/17 07:32 PM (6 years, 9 months ago) |
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Quote:
zajcob said: @ Josex
Most people seem to concur that boiling the water is sufficient for sterilization of the water. Beyond that, it seems to me that it would be sufficient to boil distilled water in a pot with the lid on, and when it is sufficiently cool, flame the needle, remove the pot lid, stick the needle into the water and draw some water into the syringe. The brief moment that you remove the lid doesn't seem like it should be much of a vector since the needle will be quickly submerged in the water and it will have been hard to have contams get too far beneath the surface of the water in that time. Also the needle can be red hot from the flaming all the way until it hits the water so it won't pick up any contams from the air.
I can see that the method you described is even cleaner and will take note of it for the future. In my specific situation I wanted a pretty quick fix, but hadn't even thought it out as much as I just described above, but after some thought, and learning that most people seem to agree that boiling water is sufficient for the water itself, I think what I just described might also do the trick. Using distilled water obviously should help by minimizing any bacteria that might no have been killed from boiling, if that is even a concern. I remember RR mentioning using distilled water in a thread, so it must have merit when working with syringes.
What you say it's a recipe for failure. It's true that water can be effectively sterilized just by boiling, but in order to keep it sterile it has to be kept in a sterile environment, obvious, no?
If you boil water in a pot and wait for it to cool in the pot, you can bet that water is no longer sterile, not to mention when you go and open the lid of the pot to suck up the water... you could just as well suck up water from a puddle in the street and call it sterile 
The purpose of using distilled water to make spore syringes is to prevent spores from germinating in the syringe, in case you want to store the syringe for later use. The minerals in well or tap water encourage spore germination. Distilled water does nothing to help keep shit more sterile.
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zajcob
Stranger

Registered: 07/28/10
Posts: 208
Last seen: 6 years, 3 months
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Re: Cultivation General Discussion [Re: Josex]
#24214181 - 04/02/17 09:30 PM (6 years, 9 months ago) |
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Regarding your earlier post, I'm not sure what you mean by a filtered container. Is this a container with a SHIP?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: zajcob]
#24214197 - 04/02/17 09:38 PM (6 years, 9 months ago) |
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a SHIP doesn't filter it's a plug
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zajcob
Stranger

Registered: 07/28/10
Posts: 208
Last seen: 6 years, 3 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#24214300 - 04/02/17 10:47 PM (6 years, 9 months ago) |
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I'm asking if it has a SHIP to stick the syringe into in addition to a filter. Like if the container is to be opened in the SAB if it just has a filter, and if so what kind of filter and also what the purpose of the filter is in this case.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: zajcob]
#24214303 - 04/02/17 10:48 PM (6 years, 9 months ago) |
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ships are for needles, if there's no ship you have to open in a SAB or in front of a flow hood
filters keep out contamination that's in the air
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zajcob
Stranger

Registered: 07/28/10
Posts: 208
Last seen: 6 years, 3 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#24214342 - 04/02/17 11:26 PM (6 years, 9 months ago) |
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I still don't see the relevance of a filter here when you don't need gas exchange since it's just a container with water that is to be drawn into the syringe, unless it is for preventing a vacuum when opening in the SAB.
Also the implication that the boiled water in the pot would be such a great vector for a syringe in the scenario I described is lost on me the way Josex puts it. The pot's interior will have been steamed and the water beneath the surface would be virtually sterile prior to opening the lid inasmuch as boiling water sterilizes it, which seems contrary to what I read in the PC manual regarding bacteria. Anyway, sure, when you open the lid of the pot, contaminants will land on the surface of the water, but beneath the surface I don't see that contaminants would have infiltrated the water in the small time frame that you remove the lid, although withdrawing water beneath the surface could suck water from the surface into the syringe. So I see the possibility of a contamination via that route, but not one as extraordinary as described. Note that this is just speculation about a last minute method that could have been done to hasten a nearly finished SAB session. Of course in general for salvaging a syringe for future use or creating new ones I would be inclined to use an SAB and other complimentary steps.
Anyway, regarding agar wedges, didn't you say that you would noc up several jars with one dish? I have five decent looking clone transfers that I want to use to inoculate 10 rye jars from, but maybe save one for further transferring/storage, giving me maybe 4 dishes to work with. I want to get some monos rolling from the clone.
And while I'm at it, regarding CVG bulk, is it common practice to just squeeze excess water with one's hand to get it to field capacity if it is too saturated?
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zajcob
Stranger

Registered: 07/28/10
Posts: 208
Last seen: 6 years, 3 months
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Re: Cultivation General Discussion [Re: zajcob]
#24214349 - 04/02/17 11:34 PM (6 years, 9 months ago) |
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Nevermind the vacuum bit since it isn't pressurized, just steaming we're talking about...
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 12 hours
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Re: Cultivation General Discussion [Re: abductee]
#24215559 - 04/03/17 02:48 PM (6 years, 9 months ago) |
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Can somebody answer my last question on this thread to keep my info together please?
https://www.shroomery.org/forums/showflat.php/Number/23857076
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Re: Cultivation General Discussion [Re: Edmunter]
#24215576 - 04/03/17 02:57 PM (6 years, 9 months ago) |
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It's odd that we don't have a forum on "cooking with mushrooms", not even for the gourmet mushrooms. No recipes or anything. Maybe I just didn't look hard enough
can I rehydrate dried cubes in warm water and then add to mushroom soup? Or maybe rehydrate then pan fry in butter? I tortured myself eating that plate of fresh cubes and next time I want it to be enjoyable. I'm thinking Campbell's Everyday Gourmet Mushroom Harvest Soup would be a good base to add some rehydrated cubes to for a yummy treat.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Should work. I don't see why not. I've made both a shroom pasta sauce and a shroom stirfry. Both were amazing
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 12 hours
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Re: Cultivation General Discussion [Re: Mad Season]
#24215671 - 04/03/17 03:25 PM (6 years, 9 months ago) |
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Powder your mushrooms and drink .2g everyday with tea. It gives you lots of energy and positive thinking. Stop 3 days b4 a big trip.
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Re: Cultivation General Discussion [Re: Edmunter]
#24216499 - 04/03/17 09:14 PM (6 years, 9 months ago) |
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Quote:
Edmunter said: Powder your mushrooms and drink .2g everyday with tea. It gives you lots of energy and positive thinking. Stop 3 days b4 a big trip.
But they taste so good when you chew them up dried. At least when you're only chewing on .2 gram, maybe not so much when you stuff a handfull in your mouth.
I bet I could make some pretty tasty dishes with cubes, I'm going to just pretend that potency isn't affected so that I can be as creative as I want with them and come up with some gourmet dish, maybe increase dose a bit so to balance potency loss.
Mushrooms are so weird on the gut, when I'm tripping I can't figure out what food is anymore... I get this feeling eventually like I want to eat and then I look in the fridge and it's like... dead stuff... ewww Then I start dreaming about mango nectar or some fancy elixer of goodness and I swear off all non hippy food for the rest of my life...
Then I sober up and eat half a dozen eggs and a pound of bacon.... it's a vicious cycle
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Quote:
Thedillestpickle said:
Mushrooms are so weird on the gut, when I'm tripping I can't figure out what food is anymore... I get this feeling eventually like I want to eat and then I look in the fridge and it's like... dead stuff... ewww
true that.
skin-on fish is the only thing that doesnt have that 'dead' inanimate edge to it. but even veggies seem ...rotten.
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Paperbagboy
Friend

Registered: 03/24/17
Posts: 163
Loc: The U.S.
Last seen: 6 years, 9 months
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Re: Cultivation General Discussion [Re: mushboy]
#24216509 - 04/03/17 09:20 PM (6 years, 9 months ago) |
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Lemon tek all the way.
Wedge of 3 maybe minus the salt.
Maybe blueberry shake and cremate the fudge vanilla capucinno
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