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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Re: Cultivation General Discussion [Re: ComebackKid]
#24211047 - 04/01/17 02:28 PM (6 years, 9 months ago) |
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Quote:
ComebackKid said: Dude crazy enough I was at a thrift store today and I happened to snap this shot.

What are the chances? Honestly this has been happening to me all day. Apologies for the derailment but April 1st has been a Trippy fuckin day full of coincidence so far and I woke up after the fools portion. The universe is making itself known today that's for sure
I agree man. Ive had the CRAZIEST nastalgic moments consecutively for the past 15 hours.
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zajcob
Stranger

Registered: 07/28/10
Posts: 208
Last seen: 6 years, 3 months
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So I was using the leftover half of a syringe to noc up ten brf jars as part of an experiment to test the merits of homemade brf vs. store bought brf, but I only got through the first eight jars before I ran out of ms solution in the syringe. I considered drawing in water that I had boiled in order to get more spores out of the syringe, but decided against it for a few reasons. First is that I had drawn air into the syringe just toying with it, but realized the air was obviously not sterile. I also wasn't sure if the boiled water would have sufficient sterility, especially since I only wanted to do it for ten or so minutes. I ultimately used a new syringe of a different variety, which I didn't want to do, but didn't feel like I had much of a choice.
Was I making the right decision not to just use water in the depleted syringe that I had just boiled for ten minutes. Would that truly have been a bad plan, or could it possibly have turned out OK?
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zajcob
Stranger

Registered: 07/28/10
Posts: 208
Last seen: 6 years, 3 months
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Re: Cultivation General Discussion [Re: zajcob]
#24211471 - 04/01/17 05:41 PM (6 years, 9 months ago) |
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I was also wondering in general what it takes to sterilize plain water? Is pressure cooking water necessary for sterility?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: zajcob]
#24211479 - 04/01/17 05:45 PM (6 years, 9 months ago) |
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boiling it?
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zajcob
Stranger

Registered: 07/28/10
Posts: 208
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Re: Cultivation General Discussion [Re: mushboy]
#24211548 - 04/01/17 06:11 PM (6 years, 9 months ago) |
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This article claims one to three minutes to "purify" it:
http://traveltips.usatoday.com/long-boil-water-purification-62933.html
Supposing I had sucked water boiled for several minutes into the syringe and I hadn't sucked any dirty air into the syringe, would that have been sufficient to get remaining spores out of the syringe without adding contaminants?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: zajcob]
#24211621 - 04/01/17 06:49 PM (6 years, 9 months ago) |
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thats basically how you make a syringe.
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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Re: Cultivation General Discussion [Re: mushboy]
#24212176 - 04/01/17 11:05 PM (6 years, 9 months ago) |
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Look okay to put to grain?
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Marmie
Peter peter portion eater



Registered: 10/21/15
Posts: 918
Loc: Deep inside the night
Last seen: 3 months, 20 days
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If you mean to suck up boiling water to get remnant spores left in the syringe , then no, the boiling water would just kill any spore in the syringe. If you mean sucking up boiling water to make a fresh syringe from a print then ya thats how its done
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Marmie]
#24212445 - 04/02/17 03:25 AM (6 years, 9 months ago) |
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He should just sterilize a little filtered container with some distilled water in it and then suck that water up with the syringe in the sab. He can steam sterilize the container with the water, no pc needed.
There should be plenty of spores yet in the empty syringe but he said he sucked up dirty air, so I understand he doesn't want to take that risk.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
Shomann said:

Look okay to put to grain?
very overgrown to know for sure. If it looked clean during colonization, chances are it's clean now
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ChadleyD
Spiralling out



Registered: 11/03/16
Posts: 19
Last seen: 5 years, 11 months
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Re: Cultivation General Discussion [Re: Josex]
#24212588 - 04/02/17 06:23 AM (6 years, 9 months ago) |
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Hello Shroomery, I noticed today that my substrate is shrinking,
 My substrate is Coir/Verm, in a monotub I have been fruiting for a couple of days and I just recently harvested (you can the bare spots in the picture.) Is there anything I should do differently? Is there anything I can do now to help re hydrate the substrate? Thank You for your help and your time. ChadleyD
-------------------- Is there anybody out there?
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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Re: Cultivation General Discussion [Re: Mad Season]
#24212754 - 04/02/17 08:44 AM (6 years, 9 months ago) |
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Quote:
Mad Season said:
Quote:
Shomann said:

Look okay to put to grain?
very overgrown to know for sure. If it looked clean during colonization, chances are it's clean now
Yeah. That last ring is new growth. I pulled this out of the fridge and let her colonize so I could use it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: ChadleyD]
#24212758 - 04/02/17 08:50 AM (6 years, 9 months ago) |
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Quote:
ChadleyD said: Hello Shroomery, I noticed today that my substrate is shrinking,
 My substrate is Coir/Verm, in a monotub I have been fruiting for a couple of days and I just recently harvested (you can the bare spots in the picture.) Is there anything I should do differently? Is there anything I can do now to help re hydrate the substrate? Thank You for your help and your time. ChadleyD
your sub will shrink as it uses water. depending on size, you can either pour some water in the corner and 'float' the sub for 30min and pour off the excess water, or if its a smaller substrate you can just mist heavily and pour off excess water. either way. check out pastys bathtub grow. that thing shrunk like crazy. cool stuff.
got any other pics of your grow?
sexy culture shomann! my ape is giving me nothing but jizz blobs on agar gonna get medieval on its ass
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: mushboy]
#24212775 - 04/02/17 08:59 AM (6 years, 9 months ago) |
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Been over two weeks and the APE plates I hit with APE syringe still haven't germinated.
Gonna make more plates and try again soon.
Also, Kenetic, if you're reading, your PE clone is colonized. Two very full quarts, and a myco quart. They're consolidating now. Not sure for how long. I'm not rushing it. Also, my TYC jars are 95% done.
I lost one TYC jar, but the other 4 TYC and 3 PE look great. Will get pics soon.
-------------------- KSSS And PE WBS.
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zajcob
Stranger

Registered: 07/28/10
Posts: 208
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Re: Cultivation General Discussion [Re: Josex]
#24212939 - 04/02/17 10:53 AM (6 years, 9 months ago) |
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@ Josex
Most people seem to concur that boiling the water is sufficient for sterilization of the water. Beyond that, it seems to me that it would be sufficient to boil distilled water in a pot with the lid on, and when it is sufficiently cool, flame the needle, remove the pot lid, stick the needle into the water and draw some water into the syringe. The brief moment that you remove the lid doesn't seem like it should be much of a vector since the needle will be quickly submerged in the water and it will have been hard to have contams get too far beneath the surface of the water in that time. Also the needle can be red hot from the flaming all the way until it hits the water so it won't pick up any contams from the air.
I can see that the method you described is even cleaner and will take note of it for the future. In my specific situation I wanted a pretty quick fix, but hadn't even thought it out as much as I just described above, but after some thought, and learning that most people seem to agree that boiling water is sufficient for the water itself, I think what I just described might also do the trick. Using distilled water obviously should help by minimizing any bacteria that might no have been killed from boiling, if that is even a concern. I remember RR mentioning using distilled water in a thread, so it must have merit when working with syringes.
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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Re: Cultivation General Discussion [Re: Moabfighter]
#24212962 - 04/02/17 11:01 AM (6 years, 9 months ago) |
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If you can't get them to germ try squirting some solution in a small jar of grain, then transfer a piece of grain to agar to clean up.
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Cultivation General Discussion [Re: zajcob]
#24212974 - 04/02/17 11:04 AM (6 years, 9 months ago) |
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What are your guys thoughts on agar>grain master>g2g vs agar>LI. I'm only thinking off about doing 10 quarts to start. Just wondering what peoples experience is with one over another. I worry about doing agar>LI cause that would be all jars being inoculated by one plate. If the culture is even the slightest bit dirty it can throw the whole run. If i go agar>masters with a couple different plates if one has any hidden contaminants the others could still fair well and be expanded if necessary. I don't know if the extra time going agar>master>g2g would be worth it or If LI would be cool for a little run.Just want to get some input.
Edited by Shineonyoucrazy (04/02/17 11:07 AM)
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Quote:
Shineonyoucrazy said: What are your guys thoughts on agar>grain master>g2g vs agar>LI. I'm only thinking off about doing 10 quarts to start. Just wondering what peoples experience is with one over another. I worry about doing agar>LI cause that would be all jars being inoculated by one plate. If the culture is even the slightest bit dirty it can throw the whole run. If i go agar>masters with a couple different plates if one has any hidden contaminants the others could still fair well and be expanded if necessary. I don't know if the extra time going agar>master>g2g would be worth it or If LI would be cool for a little run.Just want to get some input.
well that might be an issue if agar didn't make contaminates germinate. fortunately, contaminates are visible on agar. so there isn't really any risk of doing agar>li unless you do it blindfolded or just dont even look at your plates.
doing grain masters is going to take you a lot more time. do agar>li. and inspect your plate.
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Cultivation General Discussion [Re: Munchauzen]
#24213029 - 04/02/17 11:37 AM (6 years, 9 months ago) |
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Thanks munch! I think I'm going to try your blenderless LI once i grow my plates out and transfer for cleaning. Just getting back after awhile. I just started dipping my toes into agar during the end of my last run. I Just make sure to transfer until i have no doubts. I also may have to try the shoebox tubs or bods unmodified . On second thought my just try both FC's and see how they go.
-------------------- Keep your feet on the ground and your head in the clouds
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lovelaughlibs
Dopamine Slave



Registered: 10/14/15
Posts: 1,811
Loc: England
Last seen: 5 years, 11 months
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I can vouch for the blenderless LI tek being great. Just one tip as it's your first time, do NOT put the water lid on too tight before you PC it or it'll be super hard to get off and suck in a shitton of air when your crowbar efforts finally remove it. Don't ask me how I know...
-------------------- Ask and ye shall receive; Seek and ye shall find.
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