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Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
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Re: Cultivation General Discussion [Re: Rusty2096] 1
#28092059 - 12/10/22 03:48 PM (1 year, 1 month ago) |
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Quote:
Rusty2096 said:
Quote:
RoscoeReturns said:

That was your post 420 
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination π
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π° πΏ
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: Cultivation General Discussion [Re: drVetker] 2
#28092067 - 12/10/22 03:54 PM (1 year, 1 month ago) |
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Quote:
drVetker said: Monokaryotic vs Dicaryotic Mycelium
Noob here.
I'm reading the book from Mckenna on cultivation and wanted to ask you gents why is he isolating dikaryotic mycelium and inoculating grain with that as opposed to teks that just jam a syringe into the growing medium and bring the thing to fruiting stage without the necessity of selecting the dikaryotic myc, like the PF Tek for example.
Why is he doing this? Is it necessary to do this for growing on sterilized rye or can this step be skipped? Or is another tek more appropriate if this is to be skipped?
Since nobody else has answered yet I'll do my best to shed some light on it for you. *If any of this information is incorrect/incomplete please feel free to correct and educate both of us*.
You're kinda focusing on two polar opposite ends of the cultivation spectrum. 'Jamming spore syringe into sterile grain' and hoping for the best is a very bad practice that you should avoid. Spore syringes are not, and can not be sterile if you intend for the spores to be viable. The mushrooms the spores were harvested from were grown in open air, exposed to all sorts of molds and bacterias. Some syringes are cleaner than others, but NONE are devoid of contaminants. For this reason it is recommended you learn agar (which is incredible easy), or, at the very least use the BRF/PF TEK, which is slightly more resistant to contaminations. That is the 'total noob' end of the spectrum.
Isolating mono/dikaryotic strains, to the best of my knowledge, is primarily done for breeding/crossing purposes. In general, especially early on in your moco-career, you should just completely forget those words even exist. You will see a lot of beginners incorrectly talking about 'isolating a clean culture'. 99% of the time what they're really asking/talking about it simply cleaning up a culture on an agar plate. To isolate a monoculture would require literally thousands of transfers or the use of a microscope and a lot of time invested. Not to mention, you could go through all that work to isolate a monoculture and it could be a strain that doesn't even fruit or is totally bunk. Keep in mind, a *strain* is the byproduct of any two spores that pair together, NOT Golden Teacher, Penis Envy, etc. Those are 'varieties', or cultivars (sort of).
Shoot for somewhere in the middle. Run a couple BRF/PF TEK grows to get the feel for the hobby, or jump right in and learn agar, or both. Grow from spore, collect spores, take a clone, run the clone. The trick to this hobby is having a CLEAN CULTURE and having good sterile technique, mono or dikaryotic be damned.
Hope that verbal diarrhea was somewhat useful for you. I took some adderall and my brain is still stuck in 'finals week' mode lmao.
Bigfoot
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I 5318008 NOT a virgin!
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
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Re: Cultivation General Discussion [Re: Inocuole]
#28092076 - 12/10/22 04:00 PM (1 year, 1 month ago) |
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Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
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Re: Cultivation General Discussion [Re: Smellyhobbit]
#28092087 - 12/10/22 04:07 PM (1 year, 1 month ago) |
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Hey how are your jars looking SH?
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
Loc: Hole
Last seen: 2 hours, 27 minutes
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Re: Cultivation General Discussion [Re: Shrimps] 1
#28092093 - 12/10/22 04:11 PM (1 year, 1 month ago) |
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Quote:
Shrimps said: Hey how are your jars looking SH?
Inoculated today, so weβll see! I have 15 jars going, 4 have the new lids. The other 11 look good so far, but they always do right up until the final shake haha.
But yea I just inoculated the new ones about an hour ago with PEU, Averyβs Albinos, Vader, and TAT.
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Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
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Re: Cultivation General Discussion [Re: Smellyhobbit] 1
#28092100 - 12/10/22 04:18 PM (1 year, 1 month ago) |
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Vader? Never heard of those.
I am happy, got a clean plate from the Avery's Albino. It's only t1 so I will see if I will make more transfers. Going to let em grow out a bit 
Hope really your new lids will be the solution for you!
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,171
Loc: Hole
Last seen: 2 hours, 27 minutes
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Re: Cultivation General Discussion [Re: Inocuole] 2
#28092103 - 12/10/22 04:20 PM (1 year, 1 month ago) |
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π€
Vader is a cross of playa and GT. Probably my favorite mushrooms Iβve taken so far. Very strong.
Good luck with your Avery plate!
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nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
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Re: Cultivation General Discussion [Re: drVetker] 3
#28092132 - 12/10/22 04:42 PM (1 year, 1 month ago) |
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Quote:
drVetker said: Monokaryotic vs Dicaryotic Mycelium
Noob here.
I'm reading the book from Mckenna....
Welcome.
There is zero reason someone starting out here needs to know about Monokaryotic vs Dicaryotic anything.
Mckenna....that guy died 22 years ago and that book was written in 1976. I'm an old lady and I was a teenager when it was current.
It's extraordinarily out of date of current "best practices."
It was groundbreaking when written. It's mostly wrong now. He was a pioneer, but this hobby moves fast...To the point where reading that book would be like saying "I'm reading a book by Henry Ford on car production, why can't I fix my Tesla?"
Read my sig line "Things I wish I'd known when I started", that contains links to a lot of good current starter material by the people doing it best on here.
I skipped PF tek and went straight to bulk (spores to agar, agar to jars of grain, grain spawn to shoe boxes to grow shrooms) and I recommend you do too. IF YOU FOLLOW DIRECTIONS, and don't skip steps, you can do it right the first time or close to the first time.
Bottom line: only take advice from people growing lots of shrooms on here, with photos. And don't take any grow advice from a post more than 5 years old.
Since you're reading McKenna, please read my thread: To the young men taking "heroic" doses to fix themselves, but having a bad time
Katrina
-------------------- -NEW? Start here.
Edited by nektar61 (12/10/22 04:56 PM)
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RoscoeReturns
Crotchety chode man



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Re: Cultivation General Discussion [Re: Rusty2096] 4
#28092182 - 12/10/22 05:19 PM (1 year, 1 month ago) |
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Quote:
Rusty2096 said:
Quote:
RoscoeReturns said:

That was your post 420 
Perfect! Now if I never post againβ¦
Shit!
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,907
Loc: Rent free in your head
Last seen: 3 hours, 47 minutes
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Quote:
RoscoeReturns said:
Quote:
Rusty2096 said:
Quote:
RoscoeReturns said:

That was your post 420 
Perfect! Now if I never post againβ¦
Shit!
Fucking stoners
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
π
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£π
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    "Mist your balls and fan your asshole" - Pandaskis, 2023
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
RoscoeReturns said:
Quote:
Rusty2096 said:
Quote:
RoscoeReturns said:

That was your post 420 
Perfect! Now if I never post againβ¦
Shit!
You've still got 666 to look forward to! And 69,420!!
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I 5318008 NOT a virgin!
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nektar61
Into SporePlay



Registered: 07/04/20
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Re: Cultivation General Discussion [Re: Rusty2096] 3
#28092318 - 12/10/22 06:25 PM (1 year, 1 month ago) |
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Quote:
Rusty2096 said: @Nectar61 : You are somewhat forcing me to call you out here
You dare PM me a long ass unidirectional (you are not accepting PM's - at least not from me) condescending message telling me how to obey YOUR rules of the Shroomery, what to post and how to do it?
Let me be the bigger man here : FUCK OFF BITCH .....Moving on.
RUSTY:
I wrote you off board because you told a noob his grow might be contaminated when it clearly wasn't.
A TC and I quickly corrected you on board, but you seem to be standing by it.
That's not my rule. It's pretty much the biggest rule here and the only one that's really enforced. If you don't believe me, break it a few more times.
I didn't block you from messaging me. I put you on Ignore because I'm sick of going through your 30 plus posts a day of nonsense that often don't have much to do with shrooms.
I didn't know that blocks DMs, because there is a separate function to block DMs, I thought they were independent functions.
I've un-ignored you now to reply because someone told me about your comment. After you reply and tell why you're doubling down on your right to give wrong info to noobs on here, I'll put you back on Ignore so I can get some shroom shit done on here without wading through your many posts. Feel free to do same to me.
Below is the meat of what I told you, shortened, but not out of context. Feel free to post my whole note if you want though.
I did the right thing and addressed you privately, but you took it public, I'm posting here so anyone else can see it. Reply here please, this is very topical to the subject of cultivation. -----------
Rusty......
A noob giving wrong advice to a noob is against the rules and it's the one rule that's heavily enforced.
If you don't know the answer for sure from repeated personal experience, please don't give advice here. I know the desire to be helpful, ....I did it when I was new....but someone who knows will answer. Several did last night after you gave the wrong answer.
If you've seen a TC or other OG who grows lots of shrooms, answer the question elsewhere, it's fine to say "I don't know but here's a link I've seen that might answer...."
Basically the people who should answer grow questions here are people with lots of pix of fat grows of their own....
Keeping wrong answers off here is what keeps this site from becoming like the shitty reddit shroom groups, where many kids who've never seen a magic mushroom answer grow questions for reddit karma, or just to seem cool.
-------------------- -NEW? Start here.
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drVetker
Stranger

Registered: 10/25/22
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Last seen: 2 months, 21 days
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Quote:
bigfootscreepyuncl said:
Quote:
drVetker said: Monokaryotic vs Dicaryotic Mycelium
Noob here.
I'm reading the book from Mckenna on cultivation and wanted to ask you gents why is he isolating dikaryotic mycelium and inoculating grain with that ...
Since nobody else has answered yet I'll do my best to shed some light on it for you. *If any of this information is incorrect/incomplete please feel free to correct and educate both of us*....
Bigfoot
Thank you sir. I finished my first grow ever, PF tek, got only 5 g dried. I fucked it up and had only one flush. I'm not doing this again and am dealing with agar ATM.
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Screwup
Googles your dumb questions


Registered: 01/27/22
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Re: Cultivation General Discussion [Re: nektar61] 1
#28092353 - 12/10/22 06:49 PM (1 year, 1 month ago) |
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nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,241
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Re: Cultivation General Discussion [Re: drVetker]
#28092356 - 12/10/22 06:50 PM (1 year, 1 month ago) |
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Quote:
drVetker said:
Quote:
bigfootscreepyuncl said:
Quote:
drVetker said: Monokaryotic vs Dicaryotic Mycelium
Noob here.
I'm reading the book from Mckenna on cultivation and wanted to ask you gents why is he isolating dikaryotic mycelium and inoculating grain with that ...
Since nobody else has answered yet I'll do my best to shed some light on it for you. *If any of this information is incorrect/incomplete please feel free to correct and educate both of us*....
Bigfoot
Thank you sir. I finished my first grow ever, PF tek, got only 5 g dried. I fucked it up and had only one flush. I'm not doing this again and am dealing with agar ATM.
Congratulations!
Anything is good for a first grow.
Did you take spore prints? If so, you don't need to buy spores again. Or you can get some free from someone here.
-------------------- -NEW? Start here.
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drVetker
Stranger

Registered: 10/25/22
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Re: Cultivation General Discussion [Re: nektar61]
#28092397 - 12/10/22 07:20 PM (1 year, 1 month ago) |
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Quote:
nektar61 said:
Quote:
drVetker said: Monokaryotic vs Dicaryotic Mycelium
Noob here.
I'm reading the book from Mckenna....
... I skipped PF tek and went straight to bulk (spores to agar, agar to jars of grain, grain spawn to shoe boxes to grow shrooms) and I recommend you do too. IF YOU FOLLOW DIRECTIONS, and don't skip steps, you can do it right the first time or close to the first time. . . .
Katrina
Thank you very much Katrina, I just finished my first ever grow, PF tek. Only got 5 dried grams. It went sideways after the first flush. I left a print and am doing agar now with no plan to go back to PF tek, but as you said to jars of grain. Reading the book, I thought of fruiting from jars first, but I get what you're saying. I'll go into the info you have in your signature and rethink my plans.
Very kind of you, thanks again.
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nektar61
Into SporePlay



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Re: Cultivation General Discussion [Re: drVetker] 3
#28092411 - 12/10/22 07:31 PM (1 year, 1 month ago) |
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Quote:
drVetker said: Thank you very much Katrina, I just finished my first ever grow, PF tek. Only got 5 dried grams. It went sideways after the first flush. I left a print and am doing agar now with no plan to go back to PF tek, but as you said to jars of grain. Reading the book, I thought of fruiting from jars first, but I get what you're saying. I'll go into the info you have in your signature and rethink my plans. Very kind of you, thanks again.
You're welcome.
McKenna's shroom grow actually grew shrooms in quart mason jars. Big hassle when you go to harvest them. haha.
The newer "bulk" grow tek moves the grain to mason jars and the final grow to plastic shoeboxes. Not sure those existed in the 70s. I never saw them until much later.
The reason the grow can be done in shoeboxes, where you have the lid on upside down, and open it up from time to time to mist it if it's dry where you are, is some smart people (probably early on on this website, absolutely way before I was growing) figured out different than McKenna's tek.
They realized that the need for sterile conditions starts high (agar), stays pretty high for the grain jars, but once grain colonizes the whole jar, and you take it out, it's a lot harder to get contaminated, so can grow in a non hermetically sealed condition (plastic shoeboxes.) And shrooms like a little more airflow, like in a shoebox with the lid placed on top upside down.
Shrooms grow fine in the outdoors, they don't glass protection to thrive.
Here are the shoeboxes I harvested the other day. The black is spores because I let them go a little long because I wanted to get spore prints but fell asleep before picking them. They're still OK to eat and I still printed some of them.
-------------------- -NEW? Start here.
Edited by nektar61 (12/10/22 10:08 PM)
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drVetker
Stranger

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Re: Cultivation General Discussion [Re: nektar61] 1
#28092419 - 12/10/22 07:41 PM (1 year, 1 month ago) |
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Thank you
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Adas
Lonely Dreamer



Registered: 12/22/16
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Re: Cultivation General Discussion [Re: Rusty2096] 2
#28092874 - 12/11/22 04:35 AM (1 year, 1 month ago) |
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Quote:
Rusty2096 said: @Nectar61 : You are somewhat forcing me to call you out here
You dare PM me a long ass unidirectional (you are not accepting PM's - at least not from me) condescending message telling me how to obey YOUR rules of the Shroomery, what to post and how to do it?
Let me be the bigger man here : FUCK OFF BITCH
(right, that's not the definition of bigger man is it? Well, let's just call that my rule like you did )
PS: I believe this message belong in this thread considering the circumstances and also because from what I read in that message, I'd bet a lot of money she also sent similar messages to many other members.
I also believe none of you are here to put up with that kind of shit, and with respects to that, I have added her to my ignore list so you will never see me address her again in a public thread.
Moving on.
Stuff like this is better directed to the mods.
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: π
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Re: Cultivation General Discussion [Re: Adas] 1
#28092922 - 12/11/22 05:53 AM (1 year, 1 month ago) |
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You are right. They are involved. I won't go into the details because she's not worth my time and I did promise I wouldn't reply to her again or cancer this thread with what happened anymore even tho I saw her last reply which was another out of context lie but I won't reply to it. Moving on like I said in my last post.
To get back on topic : my grow room temperature is ok (room is heated) but my floor is freezing cold. The tubs that are directly on the floor at a bottom of a stack are defijetly much slower. I'll try buy and isolation mat or something to get em off the floor this week. Any cheap and ingenious ideas?
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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