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InvisibleChanga Alchemist
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24200782 - 03/28/17 07:45 PM (6 years, 9 months ago)

No shit? Dude this has got to be your issue.

Hold on to the bit of patience you've got, because I bet your sterilization was off. How are those other jars?


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:ganja::mushroom::mushroom::flyhigh:
:sporedrop:~~~:peyote::sanpedro:~~~:sporedrop:

:chugbeer:


Edited by Shomann (03/28/17 07:47 PM)


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OfflinePaperbagboy
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Re: Cultivation General Discussion [Re: Tiamo]
    #24200785 - 03/28/17 07:47 PM (6 years, 9 months ago)

Quote:

Tiamo said:
I've kept a Reishi plate since December and it looked like it was making pins, but never fruited. The yellowing is quite common, though. If you wait to loong you can't cut the mycelium anymore. Same with oysters.





How many grams of Reishi Mushrooms makes 50 grams dry?


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OfflinePinPornProducer
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Re: Cultivation General Discussion [Re: Changa Alchemist]
    #24200787 - 03/28/17 07:48 PM (6 years, 9 months ago)

Probably and old electric coil burner stove top I'm guessing.


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InvisibleChanga Alchemist
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Re: Cultivation General Discussion [Re: PinPornProducer]
    #24200791 - 03/28/17 07:50 PM (6 years, 9 months ago)

:thatsinteresting:


--------------------
:ganja::mushroom::mushroom::flyhigh:
:sporedrop:~~~:peyote::sanpedro:~~~:sporedrop:

:chugbeer:


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OfflineLobi
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Re: Cultivation General Discussion [Re: Changa Alchemist]
    #24200830 - 03/28/17 08:01 PM (6 years, 9 months ago)

Quote:

Moabfighter said:
It sure takes a long time for a big tank of a pressure cooker to heat up.

Mines been on high for 40 minutes. Still waiting on steam so I can time a 10 minute vent.



Quote:

Shomann said:
No shit? Dude this has got to be your issue.

Hold on to the bit of patience you've got, because I bet your sterilization was off. How are those other jars?




Entirely the issue. Spawn is the singular most important aspect of Mycology. Getting incomplete sterilization logs is going to effect everything. Especially if doing g2g.
Loc9 made a timelapse video of 3 month grain that had been properly PCd for 2 hours. Over the 3 months it went bacterial as shit.
When you g2g with spawn that hasn't been fully sterilized all the endospores present (thanks Bodhi) germinate and fuck off after the first or second generation. Let alone before the first flush.
This 3 month time for shit hitting the fan is perfect for us to expand a few times, fruit and harvest. By the time the substrate molds out is normally always around this time frame IME.

As stated hold on to your patience and do shit properly. Not only are you getting improper sterilization logs when you do partial steam sterilization you fuck with the water content within the jars.
Fix this vector and make sure your jars recover properly and you will be well on your way to a fat tub of :hairmetal:


--------------------
The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship.
The fellowship of atoms,
of star dust in its endless flight, of suns and worlds,  of gods and men.
The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit.

:mushroom2:My High Quality Lo-Fi Beats  -:mushroom2:MushroomCultivation Compendium  -:mushroom2:- Doing Bulk w/ No PC -:mushroom2:

:chillpill:more about my music :chillpill:


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Lobi]
    #24200836 - 03/28/17 08:03 PM (6 years, 9 months ago)

Also a lot of people improperly sterilize by never venting or inadequately venting.

Realistically even after a good ten minute vent there's still trapped air.

Class B table top autoclaves can pulse pressure while pulling a vacuum with a pump and even then only usually will claim 99% air removal.


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Invisiblevan hattonFacebookDiscord
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Re: Cultivation General Discussion [Re: bodhisatta]
    #24200915 - 03/28/17 08:20 PM (6 years, 9 months ago)

Bodhi you should of all people know that nothing is ever 100%
Literally speaking.

Fuck it buy a new table top burner (plugin) go for the medium priced ones it'll help.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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OfflineLobi
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Re: Cultivation General Discussion [Re: bodhisatta]
    #24200922 - 03/28/17 08:21 PM (6 years, 9 months ago)

Good information to know.
It is important not to confuse the act of attempting something with the actual end result you are attempting for. Have to actually be aware of cause and effect and put thought into action. Just because you are not in your SAB or in front of your hood does not mean you should act any different than you would while you are.
Every step is as important as the next and last.


--------------------
The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship.
The fellowship of atoms,
of star dust in its endless flight, of suns and worlds,  of gods and men.
The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit.

:mushroom2:My High Quality Lo-Fi Beats  -:mushroom2:MushroomCultivation Compendium  -:mushroom2:- Doing Bulk w/ No PC -:mushroom2:

:chillpill:more about my music :chillpill:


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InvisibleJosex
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Re: Cultivation General Discussion [Re: bodhisatta]
    #24200938 - 03/28/17 08:24 PM (6 years, 9 months ago)

Quote:

bodhisatta said:
Also a lot of people improperly sterilize by never venting or inadequately venting.

Realistically even after a good ten minute vent there's still trapped air.

Class B table top autoclaves can pulse pressure while pulling a vacuum with a pump and even then only usually will claim 99% air removal.




I vented for 20 min today and sterilized wheat for 3 hours at 17psi, they came out great! :datass:

SpitballJedi's quick rye prep works wonderfully with wheat as well :thumbup:


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Offlinefrunkis
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Re: Cultivation General Discussion [Re: Josex]
    #24200965 - 03/28/17 08:32 PM (6 years, 9 months ago)

how bummed should I be that my temperature/humidity thing dropped in my tub? Should I add a casing layer?



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InvisibleJosex
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Re: Cultivation General Discussion [Re: frunkis]
    #24200974 - 03/28/17 08:34 PM (6 years, 9 months ago)

It's looking happy ma nigga, no need for a casing layer.


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Josex]
    #24200989 - 03/28/17 08:42 PM (6 years, 9 months ago)

Cooker still isn't going as it should be. Vented fine. At high, took an hour to ever go crazy, turned it down half way, not doing shit.

I don't get it.


Can't imagine my oats boiling for two hours without my real two hour timer ever starting is good. They're probably cooked bad.

Feel like it may be finally about to go as it should, but fuck it's taken literally over 2 hours to get to "it might be about to work"

Glass top stove by the way. Cooker is much larger than the largest burner, but I can't do much about that


--------------------
KSSS And PE WBS.
 


Edited by Moabfighter (03/28/17 08:43 PM)


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InvisibleTheMadHatter420
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24200993 - 03/28/17 08:43 PM (6 years, 9 months ago)

DO you like have a crazy big ass PC?


--------------------
JOIN THE POW WOW


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: TheMadHatter420]
    #24201004 - 03/28/17 08:47 PM (6 years, 9 months ago)

23 QT IIRC.

Pretty big. Not big dog big. But big.


It's finally dancing a little. Tsk tsk tsk tsk, isn't spinning like its about to blow up, I'm not fucking with it.  Two hours. Start your timers.


--------------------
KSSS And PE WBS.
 


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OfflineThedillestpickle
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24201038 - 03/28/17 08:56 PM (6 years, 9 months ago)

Quote:

Moabfighter said:


Get this. I clean arms, go In box, clean plates and jars with ISO wipe, open plate, upside down, set plate on the lid, not sealed shut. Loosen jar lid. Exit box.  I flame scalpel, arms out of box. Set red hot scalpel on top of box, grab alcohol, hurry and wipe my arms, grab scalpel with my soaked ISO gloves, go in box, lube scalpel handle with my left soaked ISO glove, wipe Jar lid one last time, lift plate from lid, cut my wedge, slowly remove from plate, set plate back on lid, slowly move agar wedge 6-8 inches away to the jar, open lid with right right hand, away from the agar,so my hand opening the lid never goes over it, and as neat and easy I can plop the wedge in, out lid back on.

Rinse and repeat.




Seems like a lot of extra steps to me.  I expect your contamination is happening in the SAB, and you'll fix your issues when you get the SAB work down right. 

What's this part about the plate being upside down?  I never do that, I always keep my plates right side up.  I never "hurry" to do anything,  if I feel like I needed to hurry to do something, I try to think about what I'm doing and how I can avoid needing rush by planning ahead more.  Don't bother about wiping things more than once. 

Here's how I take transfers from my agar.  I wipe the surface of my table well with iso rag, then remove the cling-wrap from plate and place on the cleaned surface.  I loosen the jar lid so that it is still sitting on the jar but is completely loose so all i need to do is lift off.  I flame my blade and position myself ready to make the transfer.  I lift up the plate lid and bring it 2/3 of the way off the plate and then place it back down so that the bottom rim of the lid contacts the rim of the plate in 2 places and contacts the table in one place.  I apply pressure to the lid while making the cut in the agar to hold the plate steady. 

This technique does a few things for me: It gives me somewhere to put the lid, it also allows me to securely hold the plate in place while I make the cut.  Once I have my agar on the scalpel I hold it off to the side with my right hand and with my left I lift the petri lid back up and slowly replace it back over the plate.  During all of this I keep my left hand on the left side of the plate and my right hand on the right side of the plate, and it's very quick and with not a lot of motion.  I then bring my left hand over to the jar lid and lift it up, and then I bring my right hand with the scalpel over and drop the wedge. 

Actually I lied because I have never inoculated a grain jar with a wedge, but I this is exactly how I inoculate my LC jars and I have had very consistently clean LC doing it like this.


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OfflineThedillestpickle
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24201044 - 03/28/17 08:57 PM (6 years, 9 months ago)

Quote:

Moabfighter said:
23 QT IIRC.

Pretty big. Not big dog big. But big.


It's finally dancing a little. Tsk tsk tsk tsk, isn't spinning like its about to blow up, I'm not fucking with it.  Two hours. Start your timers.





You are going to turn it down though right?  You probably don't want it dancing for 2 hours straight or you could dry out your PC and warp and wreck it.  It sucks that it takes that long to heat up but once it's heated up you should still turn down your heating element to the minimum needed to keep it dancing periodically


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Thedillestpickle]
    #24201046 - 03/28/17 08:59 PM (6 years, 9 months ago)

Quote:

Thedillestpickle said:
Quote:

Moabfighter said:
23 QT IIRC.

Pretty big. Not big dog big. But big.


It's finally dancing a little. Tsk tsk tsk tsk, isn't spinning like its about to blow up, I'm not fucking with it.  Two hours. Start your timers.





You are going to turn it down though right?  You probably don't want it dancing for 2 hours straight or you could dry out your PC and warp and wreck it.  It sucks that it takes that long to heat up but once it's heated up you should still turn down your heating element to the minimum needed to keep it dancing periodically





I think I'm starting to get a feel when it's too high, I keep turning it down a CM at a time. It's down to about 2.7 right now


--------------------
KSSS And PE WBS.
 


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OfflineThedillestpickle
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24201073 - 03/28/17 09:10 PM (6 years, 9 months ago)

I'll post this picture to illustrate what I'm talking about.  I'm just using a random extra plate I have, so don't mind the water sitting on the myc. 

You need to hold the plate in place while making the cut or the plate will slide all over, so here's what I do.



I push on the lid with my finger and that holds everthing in place.  As soon as I have the wedge on the scalpel I take my left hand and replace the lid on the plate.  Now all I have to do is move my left hand over the pre-loosened jar lid on my left and lift straight up and drop the wedge from the scalpel in my right. If I am inoculating 10 jars I would remove and replace the lid of the agar plate 10 times.


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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: Thedillestpickle]
    #24201099 - 03/28/17 09:20 PM (6 years, 9 months ago)

Get a silicone pad, cutting board or dab pad, and put it on your tray in your sab or in front of your hood and put the
donor plate on the silicon pad that way it won't slide around when you go to cut it.


--------------------


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InvisibleChanga Alchemist
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Re: Cultivation General Discussion [Re: natedawgnow]
    #24201119 - 03/28/17 09:27 PM (6 years, 9 months ago)

Quote:

natedawgnow said:
Get a silicone pad, cutting board or dab pad, and put it on your tray in your sab or in front of your hood and put the
donor plate on the silicon pad that way it won't slide around when you go to cut it.





Nice. I got a few dabpadz layin' about. I'll give this a shot. Thanks for the tip:thumbup:


--------------------
:ganja::mushroom::mushroom::flyhigh:
:sporedrop:~~~:peyote::sanpedro:~~~:sporedrop:

:chugbeer:


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