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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Cultivation General Discussion [Re: Inocuole]
#23647715 - 09/15/16 02:43 PM (7 years, 4 months ago) |
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< this one has 4 bacterial jars. Can you spot them all? :P
Edited by Mad Season (09/15/16 02:50 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: Mad Season]
#23647754 - 09/15/16 02:59 PM (7 years, 4 months ago) |
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Hmm, are those yellow spots agar wedges?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Cultivation General Discussion [Re: Inocuole]
#23647761 - 09/15/16 03:00 PM (7 years, 4 months ago) |
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In the first 2 pics yes. But the last pic there's one jar with a yellow spot that's hella bacterial
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: Mad Season]
#23647774 - 09/15/16 03:04 PM (7 years, 4 months ago) |
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I see that top right one. Bottom left looks a little iffy too. Hell, all of that is way more tomentose than I'd usually go for. But, if we're talking strictly bacterial... top, second from left. Aaand.. middle, far left.
Those are my guesses. I don't like the way most of that mycelium is trying to fill in the empty space in the jars though.
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Bunya


Registered: 06/04/16
Posts: 601
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Re: Cultivation General Discussion [Re: Inocuole]
#23647784 - 09/15/16 03:06 PM (7 years, 4 months ago) |
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In the middle pic is the contam the un colonized line down the front? Im amazed where you guys spot contam most look fine to my noob eyes
-------------------- Bunya cones kill.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: Bunya]
#23647787 - 09/15/16 03:06 PM (7 years, 4 months ago) |
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I don't think the middle pic has any contams at all. Like I said it all looks kinda iffy to me based on what I'm used to but... if mad says it was clean, then it was clean.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Cultivation General Discussion [Re: Inocuole]
#23647809 - 09/15/16 03:12 PM (7 years, 4 months ago) |
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Quote:
Inocuole said: I see that top right one. Bottom left looks a little iffy too. Hell, all of that is way more tomentose than I'd usually go for. But, if we're talking strictly bacterial... top, second from left. Aaand.. middle, far left.
Those are my guesses. I don't like the way most of that mycelium is trying to fill in the empty space in the jars though.
Yeah very thick and tomentose, I put them in very early on with lots of genetics, also a few of those are pans, the ones on the ground are for sure pans. Forgot to say lol. The 4 I threw out was top right, top left, one right of the top left, and the left one on the ground
Yeah I'm pretty sure the middle pic is actually in order, oyster, cyans, and PEU
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Cultivation General Discussion [Re: Mad Season]
#23647820 - 09/15/16 03:16 PM (7 years, 4 months ago) |
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Clean spawn
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Cultivation General Discussion [Re: Mad Season]
#23647830 - 09/15/16 03:18 PM (7 years, 4 months ago) |
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:08 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: Mad Season]
#23647831 - 09/15/16 03:19 PM (7 years, 4 months ago) |
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Quote:
Mad Season said:
Quote:
Inocuole said: I see that top right one. Bottom left looks a little iffy too. Hell, all of that is way more tomentose than I'd usually go for. But, if we're talking strictly bacterial... top, second from left. Aaand.. middle, far left.
Those are my guesses. I don't like the way most of that mycelium is trying to fill in the empty space in the jars though.
Yeah very thick and tomentose, I put them in very early on with lots of genetics, also a few of those are pans, the ones on the ground are for sure pans. Forgot to say lol. The 4 I threw out was top right, top left, one right of the top left, and the left one on the ground
Yeah I'm pretty sure the middle pic is actually in order, oyster, cyans, and PEU
Oh.. you bastard, you didn't say they were different species, I was like "the fuckkk..."
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Cultivation General Discussion [Re: Inocuole]
#23647833 - 09/15/16 03:19 PM (7 years, 4 months ago) |
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What do you use pasty for grain?
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SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 3 years, 7 months
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Re: Cultivation General Discussion [Re: Pastywhyte]
#23647922 - 09/15/16 03:48 PM (7 years, 4 months ago) |
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Looks like those are milletQuote:
Pastywhyte said: Clean spawn

-------------------- AMU Q&A
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Cultivation General Discussion [Re: SloppyJoseph]
#23648658 - 09/15/16 07:58 PM (7 years, 4 months ago) |
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Quote:
SloppyJoseph said: Looks like those are milletQuote:
Pastywhyte said: Clean spawn

The master was millet. The receiving jars were wheat.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Cultivation General Discussion [Re: Pastywhyte]
#23648684 - 09/15/16 08:05 PM (7 years, 4 months ago) |
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I heard millet colonizes pretty fast
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herrenvolk


Registered: 05/14/16
Posts: 222
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Re: Cultivation General Discussion [Re: Boogieman47]
#23648848 - 09/15/16 09:26 PM (7 years, 4 months ago) |
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I have a question about agar transfers. When doing agar transfers, why do we need to cut out a whole chunk of agar and transfer it? Why not just scrape some myc off the surface and transfer that? I tried it once a while back and it seems to work OK and is easier.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: herrenvolk]
#23648863 - 09/15/16 09:37 PM (7 years, 4 months ago) |
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You can do that but it works a lot better if you transfer a wedge because the mycelium is grown through the wedge, it's taking energy from it, and it uses that energy to leap into the medium you just transferred it to. Sometimes without the wedge for leap-off, it takes a while longer to recover.
Some of the times I just scraped mycelium off the top though, it just dried up and died on the new agar before it could recover. I'm not positive that's what caused it but I stopped doing that and that stopped happening so..
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 12 hours, 58 minutes
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Re: Cultivation General Discussion [Re: Inocuole]
#23649475 - 09/16/16 03:34 AM (7 years, 4 months ago) |
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Quote:
Pastywhyte said:
Quote:
SloppyJoseph said: Looks like those are milletQuote:
Pastywhyte said: Clean spawn

The master was millet. The receiving jars were wheat.
nice clean spawn.
how do you prep your millet? Is it the same as foomans wbs prep?
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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herrenvolk


Registered: 05/14/16
Posts: 222
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Re: Cultivation General Discussion [Re: Inocuole]
#23649533 - 09/16/16 04:18 AM (7 years, 4 months ago) |
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Quote:
Inocuole said: You can do that but it works a lot better if you transfer a wedge because the mycelium is grown through the wedge, it's taking energy from it, and it uses that energy to leap into the medium you just transferred it to. Sometimes without the wedge for leap-off, it takes a while longer to recover.
Some of the times I just scraped mycelium off the top though, it just dried up and died on the new agar before it could recover. I'm not positive that's what caused it but I stopped doing that and that stopped happening so.. 
Oh that makes sense, thanks.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Cultivation General Discussion [Re: herrenvolk]
#23649578 - 09/16/16 05:20 AM (7 years, 4 months ago) |
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Quote:
herrenvolk said: I have a question about agar transfers. When doing agar transfers, why do we need to cut out a whole chunk of agar and transfer it? Why not just scrape some myc off the surface and transfer that? I tried it once a while back and it seems to work OK and is easier.
That technique is actually the best one to use when cleaning up bacterial cultures using an agar sandwich as digging into it can recontaminate the culture. But as for regular transfers, inocuole is right, you get much better leap off times if you take the wedge along with the mycelium.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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So i didnt read a tek i just sterilized foil and made prints then folded them up then saw i should have let them dry how fucked am i haha ??
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